Article
Biology
Zhongying Wang, Tatyana Gurlo, Aleksey Matveyenko, David Elashoff, Peiyu Wang, Madeline Rosenberger, Jason A. Junge, Raymond C. Stevens, Kate L. White, Scott E. Fraser, Peter C. Butler
Summary: In Type 2 diabetes, there are differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation, as revealed by phasor-FLIM. This study suggests the potential applicability of FLIM as a sensitive tool for drug discovery in diabetes by reporting perturbations in glucose metabolism in living islet cells.
COMMUNICATIONS BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Lauren K. Vasilakos, Baiba Steinbrekera, Donna A. Santillan, Mark K. Santillan, Debra S. Brandt, Daniel Dagle, Robert D. Roghair
Summary: Diabetes during pregnancy and chorioamnionitis are associated with increased levels of maternal insulin, leptin, and IL-6. While both leptin and IL-6 play roles in inflammation, they have contrasting effects on neurodevelopment. In a study of cord blood samples from 104 term infants, it was found that maternal diabetes increased levels of leptin and insulin, while chorioamnionitis was associated with elevated IL-6 levels.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Julia K. Panzer, Alejandro Caicedo
Summary: The article explores the life-threatening hypoglycemia issue in the management of type 1 diabetes and the underlying mechanisms. The authors suggest that targeting alpha-cells pharmacologically could help manage type 1 diabetes more effectively.
Article
Multidisciplinary Sciences
Shuaifei Lu, Caiyin Li, Xiaohui Jin, Leilei Zhu, Jiduo Shen, Ming Bai, Yucheng Li, Erping Xu
Summary: This study aimed to investigate the antidepressant effects of baicalin in a mouse model of depression induced by chronic unpredictable mild stress. The results showed that baicalin could alleviate depression-like behaviors by upregulating the expression of genes and improving mitochondrial function to enhance ATP levels in the brain.
Article
Multidisciplinary Sciences
Ting Lu, Rui Ma, Wenjuan Dong, Kun-Yu Teng, Daniel S. Kollath, Zhiyao Li, Jinhee Yi, Christian Bustillos, Shoubao Ma, Lei Tian, Anthony G. Mansour, Zhenlong Li, Erik W. Settles, Jianying Zhang, Paul S. Keim, Bridget M. Barker, Michael A. Caligiuri, Jianhua Yu
Summary: Engineered NK cells expressing soluble interleukin-15 and a chimeric antigen receptor targeting the spike protein of SARS-CoV-2 show promising potential as an off-the-shelf therapy for COVID-19 patients, with enhanced antiviral activity and cytotoxicity against spike protein-expressing cells.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Khalid Omer Alfarouk, Sari T. S. Alhoufie, Abdelhameed Hifny, Laurent Schwartz, Ali S. Alqahtani, Samrein B. M. Ahmed, Ali M. Alqahtani, Saad S. Alqahtani, Abdel Khalig Muddathir, Heyam Ali, Adil H. H. Bashir, Muntaser E. Ibrahim, Maria Raffaella Greco, Rosa A. Cardone, Salvador Harguindey, Stephan Joel Reshkin
Summary: COVID-19, caused by viral infection, is a pandemic disease with high mortality rate, with most symptoms related to mitochondrial dysfunction. Targeting mitochondrion may offer a more rational treatment approach, and understanding the underlying causes could enhance the opportunity to treat COVID-19 effectively.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Nicoleta Anghel, Joachim Mueller, Mauro Serricchio, Jennifer Jelk, Peter Buetikofer, Ghalia Boubaker, Dennis Imhof, Jessica Ramseier, Oksana Desiatkina, Emilia Paunescu, Sophie Braga-Lagache, Manfred Heller, Julien Furrer, Andrew Hemphill
Summary: The study investigated a novel ruthenium complex conjugated to 9-(2-hydroxyethyl)-adenine as a potential treatment against Toxoplasma gondii and Trypanosoma brucei. Proteins binding to the complex were identified using differential affinity chromatography followed by shotgun-mass spectrometry. The complex induced distinct ultrastructural modifications in the mitochondrion of the parasites, showing promising potential as a new treatment option.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Van Anh Do-Thi, Hayyoung Lee, Hye Jin Jeong, Jie-Oh Lee, Young Sang Kim
Summary: The study utilized the BacMam system to deliver IL15 and IL-15R alpha genes into murine cancer cells, resulting in the delayed growth of tumors. The cancer vaccine induced strong immune responses, specifically targeting CD8(+) effector cells, and significantly reduced Treg cells.
Article
Engineering, Biomedical
Miao Zhang, Sen Yan, Xueqin Xu, Tingting Yu, Zhaobin Guo, Ming Ma, Yi Zhang, Zhuxiao Gu, Yiwei Feng, Chunyue Du, Mengqi Wan, Ke Hu, Xiao Han, Ning Gu
Summary: A novel three-dimensional cell culture system based on hydrogels was proposed for inducing multicellular spheroid formation, which greatly enhanced glucose sensitivity and insulin content in pancreatic beta-cells compared to conventional two-dimensional conditions. The system allowed for tightly clustered monolayers or multicellular spheroids to form, promoting the expression of key factors in MIN6 cells.
Article
Biochemistry & Molecular Biology
Sevda Urcar Gelen, Seckin Ozkanlar, Semin Gedikli, Mustafa Atasever
Summary: The long-term consumption of Monosodium glutamate (MSG) can lead to metabolic syndrome and obesity, as well as increased risk of developing diabetes mellitus. In a rat study, MSG administration significantly increased the production of inflammatory factors, oxidative stress markers, and caused damage to the pancreatic cells. It was concluded that the use of MSG should be limited and controlled to prevent these adverse effects.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Tiina Ohman, Jaakko Teppo, Neeta Datta, Selina Makinen, Markku Varjosalo, Heikki A. Koistinen
Summary: The study found that there were downregulations of proteins related to mitochondrial electron transport or respiratory chain complex assembly in skeletal muscle of patients with type 2 diabetes (T2D), as well as a similar decreasing trend in muscles of impaired fasting glucose (IFG) and impaired glucose tolerance (IGT) individuals. Additionally, phosphoproteomic analysis revealed altered phosphorylation in several signaling pathways in muscles of IFG, IGT, and T2D patients.
Article
Biochemistry & Molecular Biology
Jia Wang, Ting Li, Mengling Li, Zhendong Fu, Likai Chen, Dongxing Shi, Fubin Qiu, Xintong Tan
Summary: Lycopene has been found to have beneficial effects on hepatic insulin signal transduction by alleviating mitochondrial dysfunction and up-regulating FGF21 expression under oxidative stress conditions.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Review
Medicine, Research & Experimental
Haichen Zhang, Kevin Colclough, Anna L. Gloyn, Toni I. Pollin
Summary: Monogenic diabetes, caused by mutations in a single gene, accounts for 1%-5% of diabetes cases. Correct diagnosis is crucial, but overlapping clinical features with type 1 and type 2 diabetes often lead to misdiagnosis. Improvements in sequencing technology offer opportunities for diagnosis, but challenges still remain.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Review
Cell Biology
Sarah Weksler-Zangen
Summary: This article discusses the proper diagnosis and treatment of diabetes in patients with inherited mitochondrial diseases, as well as screening for the condition in family members. The severity of mitochondrial-related diabetes depends on the location of the mutation and the number of affected mitochondria copies. The preferred treatment for diabetes in patients with mitochondrial disorders is SGLT-2i and mitochondrial GLP-1-related substances.
Article
Immunology
Yuting Tan, Wei Guo, Qi Zhu, Shihui Song, Yanni Xiang, Songjie Wu, Shi Zou, Yajun Yan, Ling Feng, Mingqi Luo, Ling Shen, Yong Feng, Ke Liang
Summary: This study investigated the function of peripheral cytokine-secreting cells in HIV/TB co-infection. The results showed that the absolute numbers of these cells were lower in patients with HIV/TB co-infection compared to TB or HIV infection alone. Additionally, the functional profiles of these cells were significantly different in HIV/TB co-infection. Furthermore, measuring the percentage of cytokine-secreting cells may be a sensitive marker for early evaluation of anti-TB treatment efficacy in HIV/TB co-infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jerzy Kotlinowski, Tomasz Hutsch, Izabela Czyzynska-Cichon, Marta Wadowska, Natalia Pydyn, Agnieszka Jasztal, Agnieszka Kij, Ewelina Dobosz, Maciej Lech, Katarzyna Miekus, Ewelina Pospiech, Mingui Fu, Jolanta Jura, Joanna Koziel, Stefan Chlopicki
Summary: The study found that Mcpip1(fl/fl)Alb(Cre) mice recapitulated most features of human PBC in terms of intrahepatic B cell issues, while showing milder symptoms in myeloid cell issues.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Letter
Biochemistry & Molecular Biology
Agata Lichawska-Cieslar, Piotr Konieczny, Weronika Szukala, Wim Declercq, Mingui Fu, Jolanta Jura
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Oliwia Kwapisz, Judyta Gorka, Agata Korlatowicz, Jerzy Kotlinowski, Agnieszka Waligorska, Paulina Marona, Natalia Pydyn, Jurek W. Dobrucki, Jolanta Jura, Katarzyna Miekus
Summary: Nonalcoholic fatty liver disease is characterized by excessive fat accumulation in the liver without excessive alcohol consumption. Chronic inflammation can lead to fibrosis, cirrhosis, and potentially hepatocellular carcinoma development. Transforming growth factor beta plays a role in inducing epithelial-mesenchymal transition and fibrosis. Studies have shown that exposure to fatty acids or a high-fat diet can lead to inflammation and changes associated with liver fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Ewelina Dobosz, Marta Wadowska, Marta Kaminska, Mateusz Wilamowski, Mohsen Honarpisheh, Danuta Bryzek, Jan Potempa, Jolanta Jura, Maciej Lech, Joanna Koziel
Summary: MCPIP-1 functions as a potent inhibitor of the inflammatory response by regulating apoptosis and retention of neutrophils, with its role in degrading antiapoptotic gene transcripts and interacting with specific miRNAs. This novel anti-inflammatory role of MCPIP-1 is crucial in balancing apoptosis and promoting the resolution of inflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Natalia Pydyn, Dariusz Zurawek, Joanna Koziel, Edyta Kus, Kamila Wojnar-Lason, Agnieszka Jasztal, Mingui Fu, Jolanta Jura, Jerzy Kotlinowski
Summary: The study demonstrates that MCPIP1 plays a crucial role in liver homeostasis. Depletion of Mcpip1 in myeloid leukocytes, leading to systemic inflammation, has a more pronounced effect on controlling liver metabolism and homeostasis than the depletion of Mcpip1 in hepatocytes.
Review
Biochemistry & Molecular Biology
Ewa Gurgul-Convey
Summary: S1P plays an important role in pancreatic insulin-secreting beta-cells and is associated with the development of diabetes. The inflammatory component of diabetes remains under-characterized, but environmental factors such as a westernized diet may participate in the enhanced inflammatory response. Disturbed lipid metabolism and polymorphisms in genes encoding S1P-generating enzymes are found to be associated with diabetes development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Katarzyna Trzos, Natalia Pydyn, Jolanta Jura, Jerzy Kotlinowski
Summary: This review discusses the etiology, risk factors, and murine models of autoimmune liver diseases, providing important references for studying their development mechanisms and testing new treatment strategies.
PHARMACOLOGICAL REPORTS
(2022)
Article
Pharmacology & Pharmacy
Judyta Gorka, Paulina Marona, Oliwia Kwapisz, Janusz Rys, Jolanta Jura, Katarzyna Miekus
Summary: This study investigated the role of MCPIP1 in ccRCC cell migration and found that MCPIP1 inhibits ccRCC cell migration by reducing the levels of Rho GTPases and the phosphorylation of FAK and Src. The loss of MCPIP1 RNase activity leads to increased expression of IL-1 beta and changes in the expression of genes correlated with migration as tumor progression occurs in ccRCC patients.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shabnam Shaabani, Louis Gadina, Ewa Surmiak, Zefeng Wang, Bidong Zhang, Roberto Butera, Tryfon Zarganes-Tzitzikas, Ismael Rodriguez, Justyna Kocik-Krol, Katarzyna Magiera-Mularz, Lukasz Skalniak, Alexander Domling, Tad A. Holak
Summary: Novel biphenyl-based chimeric compounds containing pomalidomide were found to exhibit strong inhibitory activity against PD-1/PD-L1, with compound 3 showing the best performance. Compounds 4 and 5 were able to significantly restore suppressed immunity in an ex vivo PD-1/PD-L1 blockade cell line bioassay.
Article
Pharmacology & Pharmacy
Lukasz Kaniuk, Krzysztof Berniak, Agata Lichawska-Cies, Jolanta Jura, Joanna E. Karbowniczek, Urszula Stachewicz
Summary: The enormous potential of electrospun polymer fibers in the field of biomaterials for tissue engineering and wound healing has been explored in this study. Fibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffolds were created to support the anchoring and proliferation of keratinocytes. Hyaluronic acid (HA) was used as a coating on PHBV fibers to improve wound closure processes.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Cell Biology
Paulina Marona, Judyta Gorka, Oliwia Kwapisz, Jolanta Jura, Janusz Rys, Robert M. Hoffman, Katarzyna Miekus
Summary: The study reveals that resistance to sunitinib and sorafenib in renal cell carcinoma involves different mechanisms, resulting in increased malignancy. Sunitinib resistance enhances tumor angiogenesis, while sorafenib resistance disrupts endothelial cell integrity. Both resistance mechanisms involve MCPIP1 inhibition and ccRCC progression.
CELL DEATH & DISEASE
(2022)
Article
Spectroscopy
Anna Kolodziej, Aleksandra Weselucha-Birczynska, Elzbieta Dlugon, Malgorzata Swietek, Maciej Gubernat, Lukasz Skalniak, Marta Blazewicz
Summary: This research investigated the biological potential of two types of polymer nanocomposites and studied the interaction between cells and materials using techniques such as cell proliferation, fluorescence microscopy, Raman spectroscopy, and two-dimensional correlation spectroscopy. The results showed that these nanocomposites have good biological activity and can identify the complexity of cellular response.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Gastroenterology & Hepatology
Natalia Pydyn, Justyna Kadluczka, Piotr Major, Tomasz Hutsch, Kinga Belamri, Piotr Malczak, Dorota Radkowiak, Andrzej Budzynski, Katarzyna Miekus, Jolanta Jura, Jerzy Kotlinowski
Summary: This study found that the protein levels of MCPIP1 are reduced in NAFLD patients.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Olga Mucha, Bozena Skupien-Rabian, Alicja Slota, Katarzyna Trzos, Natalia Pydyn, Bartosz Podlewski, Jolanta Jura, Jerzy Kotlinowski
Summary: MCPIP1 plays a regulatory role in adipogenesis and lipid metabolism, as well as in hepatocyte metabolism. Our study reveals that Mcpip1 deficiency leads to increased blood glucose levels during fasting and alters the expression of proteins involved in cellular adhesion and metabolic processes.
ACTA BIOCHIMICA POLONICA
(2023)
Article
Multidisciplinary Sciences
Agata Lichawska-Cieslar, Weronika Szukala, Tomasz K. Prajsnar, Niedharsan Pooranachandran, Maria Kulecka, Michalina Dabrowska, Michal Mikula, Krzysztof Rakus, Magdalena Chadzinska, Jolanta Jura
Summary: This study investigated the role of Mcpip1 in embryonic development using a zebrafish model. The expression of the zc3h12a gene encoding Mcpip1 was found to undergo dynamic changes during embryogenesis. Overexpression of wild-type Mcpip1 resulted in embryonic lethality and extensive changes in gene expression related to development.
SCIENTIFIC REPORTS
(2023)