Article
Cell Biology
Maniraj Bhagawati, Tasnim Arroum, Niklas Webeling, Ayelen Gonzalez Montoro, Henning D. Mootz, Karin B. Busch
Summary: The study reveals that the receptor subunit Tom20 interacts dynamically with the TOM core complex, with Tom20 having higher lateral mobility in the membrane than Tom7/TOM. When substrate is available, Tom20 associates with the TOM complex, leading to reduced mobility.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Review
Biochemistry & Molecular Biology
Ulfat Mohd Hanif Sayyed, Radhakrishnan Mahalakshmi
Summary: The article discusses the unique properties of the human mitochondrial outer membrane and the importance of the TOM complex. It highlights the recent research progress and the potential impact on alleviating human diseases.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Fan Jiang, Guofeng Chang, Zhenzhen Li, Mostafa Abouzaid, Xiaoyong Du, J. Joe Hull, Weihua Ma, Yongjun Lin
Summary: This study used bioinformatics approaches to evaluate the transcript levels of heat shock protein network in Chilo suppressalis under cold-induced stress. It identified highly cold-inducible proteins in C. suppressalis and found differences in transcriptional regulation between cold-acclimation and short-term cold-shock. The activation of HSP/co-chaperone network in overwintering larvae depends on their diapause state, and cold acclimation can enhance their cold tolerance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Pharmacology & Pharmacy
Evren Onay Ucar, Aslihan Sengelen, Elif Mertoglu Kamali
Summary: The study reveals that heat shock response (HSR) is closely related to therapeutic resistance of gliomas. Resveratrol (RSV) shows potential as an experimental agent for glioblastoma (GB) therapy, but the role of heat shock proteins (Hsps) in RSV efficacy remains unclear. The findings suggest that silencing Hsp27, Hsp60, Hsp70, and Hsp90 makes glioma cells more sensitive to RSV treatment, making these Hsps potential therapeutic targets for GB treatment.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Microbiology
Wei He, Gangjin Yu, Tianpeng Li, Ling Bai, Yuanyuan Yang, Zixiao Xue, Yonghao Pang, Dana Reichmann, Sebastian Hiller, Lichun He, Maili Liu, Shu Quan
Summary: Our research demonstrates the role of the periplasmic chaperone Spy in maintaining the homeostasis of certain outer membrane proteins (OMPs). Spy utilizes a unique chaperone mechanism to bind OmpX, allowing it to form a partially folded beta-strand secondary structure in a dynamic exchange of conformations. This mechanism differs from that of other E. coli periplasmic chaperones such as Skp and SurA, highlighting the differences in the mechanisms of ATP-independent chaperones.
Article
Biology
Shuo Wang, Lukas Findeisen, Sebastian Leptihn, Mark I. Wallace, Marcel Hoerning, Stephan Nussberger
Summary: Wang et al. used single-molecule measurements to uncover a potentially new mechanosensitive functionality of mitochondrial TOM core complexes. They found that TOM-CC switches reversibly between different ion permeability states associated with protein diffusion. This behavior is explained by the mechanical binding of TOM-CC to a hydrogel and the opening and closing of beta-barrel pores.
COMMUNICATIONS BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yuhei Araiso, Kenichiro Imai, Toshiya Endo
Summary: Mitochondria play a crucial role in energy production, metabolism, signaling, and apoptosis. The cell relies on translocators in the mitochondrial membranes to import mitochondrial proteins from the cytosol. Recent high-resolution structures have provided insights into the pathways and exits for the translocation of mitochondrial precursor proteins.
ANNUAL REVIEW OF BIOCHEMISTRY
(2022)
Review
Oncology
Christian Tibor Josef Magyar, Yogesh K. Vashist, Deborah Stroka, Corina Kim-Fuchs, Martin D. Berger, Vanessa M. Banz
Summary: HSP90 inhibitors may have potential efficacy in the treatment of gastrointestinal cancers, but more research is needed to determine which patient subgroups and at what time point these inhibitors may be beneficial.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
Qiang Wang, Zeyuan Guan, Liangbo Qi, Jinjin Zhuang, Chen Wang, Sixing Hong, Ling Yan, Yan Wu, Xiaoqian Cao, Jianbo Cao, Junjie Yan, Tingting Zou, Zhu Liu, Delin Zhang, Chuangye Yan, Ping Yin
Summary: Sam37 stabilizes mature Tom40 mainly through electrostatic interactions, facilitating subsequent TOM assembly. These results support the b barrel switching model and offer structural insights into the assembly and release of b barrel complexes.
Article
Veterinary Sciences
Samuel A. Atkin, Abu Saleh Md Moin, Stephen L. Atkin, Alexandra E. Butler
Summary: This study investigated the effects of hypoglycemia on the heat shock-related protein pathway. The results showed that hypoglycemia significantly decreased the expression of HSP90, 70, 27, and ubiquitin proteins, which are important for the protective response against heat stress.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Johannes M. Herrmann, Yury S. Bykov
Summary: Mitochondrial proteins are synthesized in the cytosol and targeted to different compartments within the mitochondria through specific targeting signals in their sequence. The mitochondrial import system is highly similar in yeast, mammalian cells, and even less closely related eukaryotes such as plants and trypanosomes.
Article
Biochemistry & Molecular Biology
Balint Csoboz, Imre Gombos, Zoltan Kota, Barbara Dukic, Eva Klement, Vanda Varga-Zsiros, Zoltan Lipinszki, Tibor Pali, Laszlo Vigh, Zsolt Torok
Summary: In this study, the interaction between HSPB1 and phospholipids was characterized, revealing that HSPB1 associates with membrane-forming lipids and has a strong affinity towards highly fluid membranes. HSPB1 modulates the physical properties of interacting membranes by altering lipid mobility and greatly affects the phase behavior of the plasma membrane under membrane fluidizing stress conditions. These findings suggest a new function for HSPB1 as a membrane chaperone.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Anastasia V. Poznyak, Varvara A. Orekhova, Vasily N. Sukhorukov, Victoria A. Khotina, Mikhail A. Popov, Alexander N. Orekhov
Summary: Atherosclerosis is a global health problem, leading to increased morbidity and mortality from cardiovascular diseases. Effective measures for prevention and treatment have not been developed yet. Understanding molecular interactions and the role of heat shock proteins in the progression of atherosclerosis is crucial. Some heat shock proteins have antiatherosclerotic effects, while others' roles are unclear. This review focuses on the antiatherosclerotic activity of specific heat shock proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yunxiao Dou, Yan Tan
Summary: Amyloid-beta (A beta) peptide is accumulated in mitochondria and plays a central role in Alzheimer's disease (AD) development. Exposure to aggregated A beta can damage mitochondria and dysregulate mitophagy. This study directly changed the A beta content in mitochondria and found that increased mitochondrial A beta enhances mitophagy levels. Overexpression of PreP can reverse mitochondrial A beta-induced mitophagy by regulating reactive oxygen species (ROS) and the mitochondrial membrane potential. These findings provide novel insight into the role of mitochondria-specific A beta in AD pathophysiology.
Article
Horticulture
Chao Gong, Qiangqiang Pang, Zhiliang Li, Zhenxing Li, Riyuan Chen, Guangwen Sun, Baojuan Sun
Summary: Under high temperature stress, Hsf and Hsp gene families are activated in eggplant cells, enhancing thermotolerance by removing denatured proteins. Specifically, upon exposure to heat stress, the majority of Hsf and Hsp genes in eggplant show increased expression levels.
Article
Biochemistry & Molecular Biology
Renan Yuji Miyamoto, Amanda Silva de Sousa, Plinio Salmazo Vieira, Ricardo Rodrigues de Melo, Josiane Aniele Scarpassa, Carlos Henrique Inacio Ramos, Mario Tyago Murakami, Roberto Ruller, Leticia Maria Zanphorlin
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2020)
Article
Biochemistry & Molecular Biology
Nardin Nano, Francisca Ugwu, Thiago Seraphim, Tangzhi Li, Gina Azer, Methvin Isaac, Michael Prakesch, Leandro R. S. Barbosa, Carlos H. Ramos, Alessandro Datti, Walid A. Houry
Article
Biochemistry & Molecular Biology
Natalia G. Quel, Glaucia M. S. Pinheiro, Luiz Fernando de C. Rodrigues, Leandro R. S. Barbosa, Walid A. Houry, Carlos H. Ramos
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Biochemistry & Molecular Biology
Sara A. Araujo, Gustavo H. Martins, Natalia G. Quel, Annelize Z. B. Aragao, Edna G. O. Morea, Julio C. Borges, Walid A. Houry, Maria I. N. Cano, Carlos H. Ramos
Summary: Heat shock proteins play important roles in maintaining cell proteostasis, with Hsp90 and Hsp70 interacting with a large number of proteins to assist in folding. A novel TPR-domain co-chaperone of Hsp90 was identified in Leishmania major, expressed in all developmental stages, providing a potential therapeutic target for future studies.
Article
Biochemical Research Methods
Louise U. Kurt, Milan A. Clasen, Marlon D. M. Santos, Eduardo S. B. Lyra, Luana O. Santos, Carlos H. Ramos, Diogo B. Lima, Fabio C. Gozzo, Paulo C. Carvalho
Summary: QUIN-XL is a software that utilizes unsupervised clustering to group cross-link identifications based on their quantitative profiles across multiple samples, highlighting regions of protein or system conformational changes when comparing different biological conditions. The software's clusters directly correlate with known protein 3D structures of different conformers, validating its utility in analyzing protein structures and interactions.
Article
Biochemistry & Molecular Biology
Josielle Abrahao, Barbara T. Amaro, Barbara R. Peres, Natalia G. Quel, Annelize Z. B. Araga, Edna G. O. Morea, Maria Isabel N. Cano, Walid A. Houry, Carlos H. I. Ramos
Summary: ATPases from the AAA+ superfamily are crucial for diverse cellular activities, with Rvbs identified as important components in protein complex assembly. This study focused on characterizing Rvbs from Leishmania major, revealing their ATPase activity and expression throughout different life stages. By obtaining specific antibodies against LmRUVBLs, the study provides valuable insights for potential intervention strategies against Leishmaniasis.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Natalia G. Quel, Luiz Fernando de C. Rodrigues, Annelize Z. B. Aragao, Glaucia M. S. Pinheiro, Rafael P. Camacho, Denio E. P. Souto, Lauro T. Kubota, Leandro R. S. Barbosa, Carlos H. I. Ramos
Summary: SGTs proteins are co-chaperones that play crucial roles in the cellular PQC system, with differences between them to be better understood through studying other orthologous proteins; AaSGT protein aids in understanding the PQC system in Aedes aegypti, which is significant for developing strategies to inhibit the vector of neglected diseases.
Review
Biochemistry & Molecular Biology
Carlos H. Ramos, Kehinde S. Ayinde
Summary: Drug repositioning is a promising strategy that utilizes approved drugs to reduce risks and costs. It may provide a new therapeutic approach for viral diseases.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Paulo Roberto Dores-Silva, Vanessa Thomaz Rodrigues Kiraly, Milene Nobrega de Oliveira Moritz, Vitor Hugo Balasco Serrao, Patricia Maria Siqueira dos Passos, Valentine Spagnol, Felipe Roberti Teixeira, Lisandra Marques Gava, David Mario Cauvi, Carlos Henrique Inacio Ramos, Antonio De Maio, Julio Cesar Borges
Summary: The study revealed that hHep1 can prevent the formation of HSPA SMAs under thermal stress and enhance HSPA ATPase activity in both monomeric and preformed SMA states, as well as remodel HSPA9 and HSPA1A SMAs into smaller forms. Additionally, hHep1 was found to interact with mitochondria, nucleus, and liposomes resembling mitochondrial membranes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Thiago Seraphim, Nardin Nano, Yiu Wing Sunny Cheung, Siripat Aluksanasuwan, Carolina Colleti, Yu-Qian Mao, Vaibhav Bhandari, Gavin Young, Larissa Holl, Sadhna Phanse, Yuliya Gordiyenko, Daniel R. Southworth, Carol Robinson, Visith Thongboonkerd, Lisandra M. Gava, Julio C. Borges, Mohan Babu, Leandro R. S. Barbosa, Carlos H. Ramos, Philipp Kukura, Walid A. Houry
Summary: The R2TP complex, formed by RUVBL1 and RUVBL2 ATPases, associates with PIH1D1 and RPAP3 proteins and plays a role in promoting the formation of macromolecular complexes. RPAP3 is identified as the central subunit of R2TP, linking PIH1D1 and RUVBL1/2. The study provides insights into the structure and function of the R2TP complex.
Article
Multidisciplinary Sciences
Conrado C. Goncalves, Itai Sharon, T. Martin Schmeing, Carlos H. Ramos, Jason C. Young
Summary: Under stress conditions, misfolded polypeptides in human cells can form potentially cytotoxic insoluble aggregates which can be eliminated by HSP70 chaperone machinery. Human HSPB1 plays a role in disaggregation, co-aggregating with unfolded protein substrates to promote efficient disaggregation and refolding led by HSP70.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Noeli Soares Melo Silva, Luiz Fernando de Camargo Rodrigues, Paulo Roberto Dores-Silva, Carlos Alberto Montanari, Carlos Henrique Inacio Ramos, Leandro Ramos Souza Barbosa, Julio Cesar Borges
Summary: The study reveals that rHSPA8 shares structural and functional similarities with other human Hsp70s, interacts with adenosine nucleotides, has lower ATPase activity compared to HSPA5/hGrp78/hBiP but higher than recombinant HSPA1A/hHsp70-1A, and shows capacity in preventing the aggregation of client proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Kehinde S. Ayinde, Glaucia M. S. Pinheiro, Carlos H. I. Ramos
Summary: This article describes the interaction between ORF9b, an important accessory protein of SARS-CoV-2, and TOM70, a member of the mitochondrial translocase of the outer membrane complex. The study explores the role of this interaction in immune evasion and inhibition of the interferon activation pathway.
Article
Biochemistry & Molecular Biology
Juliana C. Franco, Maria L. C. Nogueira, Gabriela M. Gandelini, Glaucia M. S. Pinheiro, Conrado C. Goncalves, Leandro R. S. Barbosa, Jason C. Young, Carlos H. I. Ramos
Summary: Perturbation of protein structure under cellular stress can lead to aggregate formation. Plants possess HSP100 for disaggregation, while animals utilize a system composed of HSP70/HSP40 and HSP110. This study cloned and investigated a putative HSP110 from Sorghum bicolor, showing similar structure and functional activities with human HsHSP110.
Article
Biochemistry & Molecular Biology
Jemmyson R. de Jesus, Leonardo A. Linhares, Annelize Z. B. Araga, Marco A. Z. Arruda, Carlos H. I. Ramos
Summary: The imbalance in metal homeostasis can lead to human diseases and exposure to higher metal concentrations can cause cellular stress and toxicity. Therefore, understanding the cytotoxic effect of metal imbalance is crucial for uncovering the biochemical mechanism of homeostasis and the role of protective proteins against metal toxicity. This study found evidence suggesting the involvement of cochaperones from the Hsp40/DNAJA family in metal homeostasis, potentially through modulating the activity of Hsp70. The research focused on the effect of zinc and copper on the human Hsp40 cochaperone DNAJA1, showing that zinc removal affected its stability and chaperone function, while the reintroduction of zinc restored its native properties and copper partially restored them.