Article
Chemistry, Applied
Bo Chen, Xiaoyan Liu, Kai Zhou, Yong Xie, Ying Wang, Hui Zhou, Yanhong Bai, Baocai Xu
Summary: The study investigated the effects of modulating hydrophobic interaction and disulfide bond on the properties of myofibrillar protein emulsion gels at high temperature. It also differentiated the contribution of non-covalent (hydrophobic interaction) and covalent intermolecular interactions (disulfide bond) to interfacial protein films. The results showed that modulating hydrophobic interaction or disulfide bonds improved the properties of the myofibrillar protein emulsion gels at 95 degrees C, with changes in interfacial adsorption patterns and emphasis on gel matrix improvement and stiffness increase of interfacial protein films.
Article
Chemistry, Multidisciplinary
Chaemyeong Lee, Yeil Kim, Minji Kim, Hyeri Yoo, Eunji Sim, Sang-Yup Lee
Summary: In this study, we investigate the substantial alteration of molecular self-assembly suprastructure by the covalent bonds linking individual building block molecules. The role of covalent bonding within self-assembled structures of cysteinyl bolaamphiphile is focused on, and the contribution of disulfide bonds in facilitating the bending of nanobelt assembly and the formation of longer assembled structures is revealed. Molecular dynamics simulation confirms the anisotropic distribution of disulfide bonds causing the bending of nanobelt assembly.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Rui Fu, Haoqiang Song, Xingjiang Liu, Yongqiang Zhang, Guanjun Xiao, Bo Zou, Geoffrey I. N. Waterhouse, Siyu Lu
Summary: A microwave-assisted strategy was reported for the preparation of solid-state fluorescent multi-color carbon dots (SFM-CDs) with blue, yellow, and red emissions from the same precursors within 5 minutes. The SFM-CDs showed excellent solid-state fluorescence performance and were used as the fluorescent converter layer in multi-color LEDs and white LEDs with a high color rendering index.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Food Science & Technology
Xia Gao, Jirawat Yongsawatdigul, Runlin Wu, Juan You, Shanbai Xiong, Hongying Du, Ru Liu
Summary: The study demonstrated the varying effects of high intensity ultrasound pre-treatment modes on the gelation properties of silver carp surimi. Pre-treatment before salt-chopping resulted in improved water-holding capacity and puncture properties, while treatments after salt-chopping or casing stuffing had minimal impact on gelation properties.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biophysics
Veronika Dzupponova, Gabriel Zoldak
Summary: In this study, we investigated the aggregation mechanism and structures of pathological human multiple myeloma light chain aggregates (hLC) after disrupting stabilizing disulfide bonds using various reducing agents. We found that the aggregation kinetics can be described by two macroscopic rate constants related to nucleation and growth. Surprisingly, the growth rate constants decreased at higher protein concentrations due to the involvement of an aggregation active monomer particle that is successively depleted. Seeding experiments demonstrated the specificity of the aggregates and three-dimensional visualization showed variable, reducer-specific branched morphologies.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Materials Science, Multidisciplinary
Rongsheng Gao, Lingxiao Li, Xueyan Liu, Chengyou Kan
Summary: A novel fluorescent molecule DADENA with disulfide bond and amino was synthesized and its spectral properties were studied. The PU-DADENA nanoparticles showed good transparency, uniformity, and high stability, with almost 100% reduction conversion of disulfide bonds. DADENA and PU-DADENA nanoparticles have potential applications in fluorescent cell marking, cancer detection, and drug delivery.
Article
Chemistry, Analytical
Jasvir Kaur, Prabhat K. Singh
Summary: In this study, a simple, convenient, rapid and selective method using a molecular rotor probe Thioflavin-T (ThT) has been developed to detect sodium hexa-meta-phosphate (HMP) in food. The sensing method is based on the aggregation induced emission of ThT by HMP, resulting in the formation of a fluorescent ThT-HMP aggregate complex. The method showed high selectivity and sensitivity towards HMP, and was successfully applied to detect HMP in real water samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Food Science & Technology
Xin Kong, You Li, Xinqi Liu
Summary: Soybean trypsin inhibitor (STI) was recovered from soybean whey using ammonium sulfate salting and purified to a 5.46-fold increase. Further purification using a Superdex 75 increase gel filtration column resulted in a 91.5% electrophoretically pure soybean Kunitz trypsin inhibitor (SKTI) with a purification fold of 33.32 and a specific activity of 1072.9 U/mg. SKTI exhibited good stability, maintaining 85.65% inhibitory activity after heating at 80 degrees C for 30 min and showing no significant change in activity over a pH range of 3-10.
Article
Agriculture, Multidisciplinary
Ying Zhang, Min Song, Jiyuan Xu, Xin Li, Anshu Yang, Ping Tong, Zhihua Wu, Hongbing Chen
Summary: This study focused on allergen aggregations (AAs) in roasted peanuts and investigated their structural changes and allergenic potential. Results showed that AAs were strongly recognized by IgE and cross-linked mainly by disulfide bonds (DBs). Different types of DB rearrangement in AAs were identified, including interprotein, intraprotein, and loop-linked DBs. Ara h 2 and Ara h 6 were found to have the most cysteine residues for cross-linking. Chemical modification was predominantly observed in Ara h 1 and Ara h 3, and it altered the local structures of proteins, potentially affecting their allergenicity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Applied
Yaoyao Lian, Ya Li, Ruyan Lv, Lifeng Wang, Wenfei Xiong
Summary: This study found that alkali concentration has a significant impact on the gel formation of rice protein, with higher concentration leading to faster gel formation. Heating can accelerate the gel formation rate, but prolonged heat treatment causes gel liquefaction. Alkali can cause depolymerization of glutelin and form a gel network, while higher alkali concentration contributes to the gel network mainly through the formation of disulfide bonds.
FOOD HYDROCOLLOIDS
(2024)
Article
Biochemistry & Molecular Biology
Kosuke Oyama, Makoto Nakakido, Takatoshi Ohkuri, Hitomi Nakamura, Kouhei Tsumoto, Tadashi Ueda
Summary: Researchers successfully created a new mutant, mut10, of the CH2 domain by introducing a disulfide bond, followed by a more advanced mutant, mut25, by combining mut10 and mut20. Mut25 exhibited enhanced thermal stability, increased resistance to enzymatic digestion, and reduced aggregation compared to the original CH2 domain and mut20. It is the most stable variant of the humanized whole CH2 domains reported so far, and has the potential to serve as a new platform for antibody therapeutics due to its ability to reduce immunogenicity by decreasing aggregation.
Article
Biochemical Research Methods
Vanessa Wong, Laura Pascual Fernandez, Praveen Kallamvalli Illam Sankaran, Alexander Holt, Rahul Mishra, Vaishali Sinha, Viv Lindo
Summary: This study presents a three-step approach to detect, confirm, and characterize partially reduced fragment species in an IgG1 monoclonal antibody. Microchip capillary electrophoresis and high-performance size exclusion chromatography were used for fast screening, while capillary electrophoresis-sodium dodecyl sulphate and reverse phase high-performance liquid chromatography coupled with mass spectrometry were employed for confirmation and characterization. The study provides a systematic strategy for process development and investigation of anomalous events in manufacturing, and compares modern and conventional analytical techniques for fragment analysis.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Patricia Haremski, Lars Epple, Matthias Wieler, Piero Lupetin, Leonid Klinger, Eugen Rabkin, Michael J. Hoffmann
Summary: This study investigates surface self-diffusion in nickel bicrystals through experiments and numerical simulations. The results indicate that surface diffusion is the dominant mechanism of grain boundary grooving and the anisotropy of surface diffusion coefficients is determined accurately.
Article
Chemistry, Applied
Nerea Munoz-Almagro, Mar Villamiel, Peter J. Wilde, A. Patrick Gunning, Antonia Montilla
Summary: This study investigated the rheological properties of gels formed with sunflower pectins under different concentrations of Ca2+, sucrose or sweeteners, and pH levels. The results showed that MF-pectin formed the firmest gel under various conditions, while UF-pectin combined with sweeteners at pH 3.0 produced the strongest gels. The findings suggest the potential of using stevia and saccharin in the development of low-calorie and low-glycemic index gels from sunflower pectin for a wide range of food applications.
FOOD HYDROCOLLOIDS
(2021)
Article
Chemistry, Multidisciplinary
Chen Li, Ting Wang, Mengting Fan, Ning Wang, Xiaofeng Lin, Yan Sun, Xiaoyan Cui
Summary: Silicon-substituted coumarin (SiC) is an important family of fluorescent probes, both intramolecularly and intermolecularly enhanced by hydrogen bonds (H-bond), for sensitively tracking proteins in vivo through the assembly and disassembly of nanoaggregates. SiC with intramolecular H-bond exhibits significant aggregation, solvatochromism, and strong fluorescence with a red-shifted spectra in polar, protic environments. Without additional organic linkers, the compact skeleton of SiC with H-bond ensures sensitive and selective sensing of targeted proteins through efficient disassembly of the aggregates. In the presence of strong intermolecular H-bonds with the target protein pocket, SiC shows fluorescence enhancement as high as >250-fold. Selectively tracking proteins in complex biological systems has confirmed SiC as a versatile skeleton for sensitive monitoring of proteins.
Meeting Abstract
Biophysics
Sarthak Arora, Rajaram Swaminathan
BIOPHYSICAL JOURNAL
(2018)
Editorial Material
Chemistry, Physical
Amitava Chandra, Amitabha Chattopadhyay, Richard Cogdell, Ankona Datta, Arijit De, Shaina Dhamija, Murali Golla, Stefan Haacke, Mahesh Hariharan, John Helliwell, Roberto Improta, Ramapurath S. Jayasree, Mike Jones, Joshy Joseph, Tolga Karsili, Bern Kohler, Retheesh Krishnan, Imon Mandal, Dimitra Markovitsi, Himani Medhi, Padmaja P. Mishra, Priyadarshi Roy Chowdhury, Manas Sarangi, Ilme Schlichting, John Seddon, Amit Sharma, Atchimnaidu Siriki, Rajaram Swaminathan, Rienk van Grondelle, Reji Varghese, Ravindra Venkatramani, Anthony Watts
FARADAY DISCUSSIONS
(2018)
Editorial Material
Chemistry, Physical
Vinayak Bhat, Richard Cogdell, Carlos E. Crespo-Hernandez, Ankona Datta, Arijit De, Stefan Haacke, John Helliwell, Roberto Improta, Joshy Joseph, Tolga Karsili, Bern Kohler, Retheesh Krishnan, L. Mahil, Frederick Lewis, Imon Mandal, Dimitra Markovitsi, Padmaja P. Mishra, Sneha Paul, Ganga Periyasamy, P. I. Pradeepkumar, Priyadarshi Roy Chowdhury, Manas Sarangi, Devika Sasikumar, Igor Schapiro, Gebhard F. X. Schertler, Ilme Schlichting, Javier Segarra-Marti, Rajaram Swaminathan, V. Vishnu, Rienk Van Grondelle, Reji Varghese, Ravindra Venkatramani
FARADAY DISCUSSIONS
(2018)
Article
Chemistry, Physical
Mohd. Ziauddin Ansari, Amrendra Kumar, Dileep Ahari, Anurag Priyadarshi, Padmavathi Lolla, Rashna Bhandari, Rajaram Swaminathan
FARADAY DISCUSSIONS
(2018)
Editorial Material
Chemistry, Physical
Amitabha Chattopadhyay, Richard Cogdell, Carlos E. Crespo-Hernandez, Ankona Datta, Arijit De, Stefan Haacke, Mahesh Hariharan, John Helliwell, Ashley Hughes, Roberto Improta, Mike Jones, Joshy Joseph, Tolga Karsili, Bern Kohler, Retheesh Krishnan, Anvy Kuriakose, L. Mahil, Dimitra Markovitsi, Himani Medhi, Ganga Periyasamy, P. I. Pradeepkumar, Priyadarshi Roy Chowdhury, Manas Sarangi, Igor Schapiro, Gebhard F. X. Schertler, Ilme Schlichting, Javier Segarra-Marti, Rajaram Swaminathan, V. Vishnu, Rienk Van Grondelle, Ravi Kumar Venkatraman, Ravindra Venkatramani, Anthony Watts
FARADAY DISCUSSIONS
(2018)
Article
Biochemistry & Molecular Biology
Neelakant Varma, Inderbhan Singh, Mohinder Singh Dahiya, Vijay Kumar Ravi, Satish Kumar
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2018)
Article
Biochemistry & Molecular Biology
Neelakant Varma, Himanshi Kukrety, Vijay K. Ravi, Satish Kumar
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2020)
Article
Medicine, Legal
Vivek Sahajpal, Abhishek Singh, Mukesh Thakur, Arun Sharma, Kailash Chandra, Satish Kumar
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2020)
Article
Medicine, Legal
Vivek Sahajpal, Abhishek Singh, Mukesh Thakur, Arun Sharma, Deepika Bhandari, Lalit Kumar Sharma, Kailash Chandra, Satish Kumar
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Mohd Ziauddin Ansari, Rajaram Swaminathan
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2020)
Article
Chemistry, Physical
Amrendra Kumar, Dileep Ahari, Anurag Priyadarshi, Mohd Ziauddin Ansari, Rajaram Swaminathan
JOURNAL OF PHYSICAL CHEMISTRY B
(2020)
Article
Genetics & Heredity
Abhishek Singh, Vivek Sahajpal, Satish Kumar, Malay Shukla, Deepika Bhandari, Shivkant Sharma, Arun Sharma, Kailash Chandra, Lalit Kumar Sharma, Mukesh Thakur
Summary: In the Indian montane system, human populations exhibit a unique social organization with infrequent inter-caste marriages, affecting demography and population genetic structure; this study genotyped 594 unrelated individuals from diverse populations in Himachal Pradesh and found deep genetic admixture among different castes.
Article
Biophysics
Neelakant Varma, Abhishek Singh, Vijay K. Ravi, Mukesh Thakur, Satish Kumar
Summary: Deltamethrin interacts with Hen Egg White Lysozyme in vitro, inducing protein aggregation and altering its conformation, subsequently affecting the catalytic activity of the protein.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Medicine, Legal
Abhishek Singh, Vivek Sahajpal, Satish Kumar, Malay Shukla, Deepika Bhandari, Shivkant Sharma, Lalit Kumar Sharma, Mukesh Thakur
Summary: The study investigated the genetic and forensic attributes of the Uttarakhand population using 20 autosomal STR loci in the PowerPlex (R) 21 System. Results revealed Penta E locus as the most polymorphic and discriminative, with higher heterozygosity within the population. Phylogenetic analysis showed a higher genetic affinity of the Uttarakhand population with the neighboring Himachal Pradesh population.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Rajat Anand, Manish Agrawal, Venkata Satish Kumar Mattaparthi, Rajaram Swaminathan, Sitangshu Bikas Santra