Journal
BIOCHEMICAL GENETICS
Volume 47, Issue 11-12, Pages 775-788Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10528-009-9276-9
Keywords
5S rDNA; Nontranscribed spacer variations; Cluster organization; Razor clam; Ensis macha
Funding
- PFShare-90
- European Community
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For a study of 5S ribosomal genes (rDNA) in the razor clam Ensis macha, the 5S rDNA region was amplified and sequenced. Two variants, so-called type I or short repeat (similar to 430 bp) and type II or long repeat (similar to 735 bp), appeared to be the main components of the 5S rDNA of this species. Their spacers differed markedly, both in length and nucleotide composition. The organization of the two variants was investigated by amplifying the genomic DNA with primers based on the sequence of the type I and type II spacers. PCR amplification products with primers EMLbF and EMSbR showed that the long and short repeats are associated within the same tandem array, suggesting an intermixed arrangement of both spacers. Nevertheless, amplifications carried out with inverse primers EMSinvF/R and EMLinvF/R revealed that some short and long repeats are contiguous in the same tandem array. This is the first report of the coexistence of two variable spacers in the same tandem array in bivalve mollusks.
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