Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 79, Issue -, Pages 94-103Publisher
ELSEVIER
DOI: 10.1016/j.bej.2013.07.006
Keywords
Lignocellulosic ethanol; Kluyveromyces marxianus; Laccase detoxification; Steam explosion; Process configurations; Substrate loadings
Funding
- RESTOENE project (Consejeria de Educacion - Comunidad de Madrid, Spain)
- CPI contract (Consejeria de Educacion-Comunidad de Madrid, Spain)
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In our previous work, the ability of laccase enzymes to improve the fermentation performance of the thermotolerant yeast Kluyveromyces marxianus CECT 10875 on steam-exploded wheat straw slurry was demonstrated. As a continuation of this study, the present research evaluates different aspects, including pretreatment conditions, process configurations and substrate loadings, with the aim to proceed towards the use of K. marxianus and laccases for second generation ethanol production. For it, two wheat straw slurries resulting from different steam explosion pretreatment conditions (200 degrees C, 2.5 min and 220 degrees C, 2.5 min) were employed at various substrate loadings [5-14% (w/v)] under two process configurations: SSF (simultaneous saccharification and fermentation) and PSSF (presaccharification and simultaneous saccharification and fermentation). The better performance of K. marxianus was observed on the slurry produced at softer conditions. Its lower inhibitors content allowed to increase the total solids loading up to 10% (w/v) in both process configurations, reaching higher ethanol concentrations (12 g/L). Moreover, laccase detoxification improved these results, particularly in SSF processes, increasing the substrate loading up to 12% (w/v) and, consequently, obtaining the highest ethanol concentration (16.7 g/L). (C) 2013 Elsevier B.V. All rights reserved.
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