Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 74, Issue -, Pages 115-120Publisher
ELSEVIER
DOI: 10.1016/j.bej.2013.02.014
Keywords
Delila; Rosea1; Phenylpropanoid metabolism; Salvianolic acid B; Rosmarinic acid; Salvia miltiorrhiza
Funding
- Fundamental Research Funds for the Central Universities [2010ZYGX028]
- National Natural Science Foundation of China [31270338]
- Ministry of Technique and Science, PR China [2006BAI06A12-04]
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Phenolic acids are universally distributed in plant species, where they participate in numerous bioactivities. However, their low abundance in Salvia miltiorrhiza is a perplexing problem. We examined the heterologous expression of two transcription factors, Delila (DEL) and Rosea1 (ROS1), from Antirrhinum majus in genetically modified S. miltiorrhiza. Productions of rosmarinic acid (RA) and salvianolic acid B (Sal B) were significantly elevated during normal growth stages. Contents of other phenylpropanoid metabolites were enhanced and antioxidant activity was markedly stronger. Both SmCHS and SmRAS were stimulated by co-expression of DEL and ROS1. Because levels of phenylpropanoid metabolites were increased while both were co-expressed in S. miltiorrhiza, we believe that this will be a useful strategy for improving the contents of phenolic acids (especially RA and Sal B) in this species. (C) 2013 Elsevier B.V. All rights reserved.
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