Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 56, Issue 1-2, Pages 94-106Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2011.05.012
Keywords
Enterococcus faecium MMRA; Enterocin A; Realkalized batch; Fed-batch; Fermentation; Modelling; Temperature; pH
Funding
- Spanish Agency for International Cooperation (AECI), Spain [A/5664/06]
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The growth, nutrient (total sugars, phosphorous, nitrogen and proteins) consumption and product formation by Enterococcus faecium MMRA in de Man. Rogosa and Sharpe (MRS) broth was followed in non-realkalized batch. realkalized batch and realkalized fed-batch cultures. In the latter fermentation, the growing culture was fed with a mixture of MRS medium (20 g of glucose/L) and a 400 g/L concentrated glucose. In the three cultures, a typically homolactic fermentation (only lactic acid was produced) was observed. The realkalized fed-batch culture was mainly characterized with higher biomass (2.4 g/L), lactic acid (32.5 g/L) and enterocin A (35.9 AU/mL) concentrations compared with the two batch processes. Mathematical models were developed to describe the productions of biomass, enterocin A and lactic acid in the two realkalized cultures. The growth and lactic acid production were successfully modelled with the Monod and the Luedeking and Piret models, respectively. Enterocin A was modelled by using a modified form of the Luedeking and Piret model, which includes a term for the effect of the pH drop rate on bacteriocin synthesis. Furthermore, the maximum enterocin A stability was obtained at temperatures below 100 degrees C, at acidic pH values and short incubation times. (C) 2011 Elsevier B.V. All rights reserved.
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