Article
Biochemistry & Molecular Biology
Lian Yang, Qing Zhang, Yili Yang, Qun Wang
Summary: The study investigated the therapeutic effects of PYR-41 on atopic dermatitis. The results showed that PYR-41 can activate the NF-?B signaling pathway by reducing the ubiquitination levels of specific molecules and inhibiting the degradation of I?Ba. In addition, in a mouse model, PYR-41 treatment significantly improved symptoms and reduced the expression of inflammatory markers.
Article
Biochemistry & Molecular Biology
Hitoshi Sawada, Shukumi Inoue, Takako Saito, Kei Otsuka, Maki Shirae-Kurabayashi
Summary: The extracellular ubiquitin-proteasome system plays a role in sperm binding to and penetration of the vitelline coat during fertilization in ascidian Halocynthia roretzi. The inhibitor of UBA strongly inhibits the fertilization process in H. roretzi. The UBA1 and UBA6 genes are found to be expressed in the ovary and testis and may be involved in the ubiquitination of HrVC70.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Guanzheng Liu, Jiefeng Yu, Runqiu Wu, Lin Shi, Xu Zhang, Wanhong Zhang, Xiaomin Zhong, Yifeng Wang, Huan Li, Yang Shen, Changyong Wu, Rutong Yu, Mingshan Niu, Xuejiao Liu
Summary: The study identified a potential therapeutic strategy by inducing UPR in GBM cells through UBA1 inhibition. This approach showed significant anti-tumor effects in animal models, with a greater impact on GBM stem cells, and highlighted GRP78 as a potential molecular marker for personalized treatment targeting UBA1.
CELL DEATH & DISEASE
(2021)
Article
Medicine, Research & Experimental
Mengyang Wang, Xue Han, Tianxiang Yu, Minxiu Wang, Wu Luo, Chunpeng Zou, Xiuyun Li, Gao Li, Gaojun Wu, Yi Wang, Guang Liang
Summary: Understanding the molecular mechanisms of deleterious cardiac remodeling is important for the development of treatments for heart failure. Recent studies have highlighted a role of deubiquitinating enzymes in cardiac pathophysiology. In this study, we screened for alteration of deubiquitinating enzymes in experimental models of cardiac remodeling and found a potential role of OTUD1.
Article
Chemistry, Multidisciplinary
Gaku Akimoto, Arianna P. Fernandes, Jeffrey W. Bode
Summary: Ubiquitylation is a process that attaches ubiquitin to proteins in eukaryotic cells and involves multiple enzymes. We have developed a method to transfer ubiquitin specifically to target proteins, which is important for studying protein function.
ACS CENTRAL SCIENCE
(2022)
Article
Medicine, Research & Experimental
Yang Gong, Bin Kong, Wei Shuai, Tao Chen, Jing Jing Zhang, He Huang
Summary: The study shows that USP38 plays an important role in ventricular remodeling and arrhythmias after myocardial infarction. Cardiac-specific USP38 knockout reduces the inflammatory response, improves cardiac remodeling, and reduces susceptibility to arrhythmias, while USP38 overexpression has the opposite effect. These findings suggest that USP38 may be an important target for treating cardiac remodeling and arrhythmias after myocardial infarction.
Article
Biochemistry & Molecular Biology
Mireia Perera-Gonzalez, Attila Kiss, Philipp Kaiser, Michael Holzweber, Felix Nagel, Simon Watzinger, Eylem Acar, Petra Lujza Szabo, Ines Fonseca Goncalves, Lukas Weber, Patrick Michael Pilz, Lubos Budinsky, Thomas Helbich, Bruno Karl Podesser
Summary: Pressure overload leads to decreased cardiac function and LV dilation, with overexpression of TN-C playing a key role in this process. Reverse remodeling partially improves cardiac function and reduces fibrosis; however, TN-C protein expression remains unchanged.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hannah E. Ledvina, Qiaozhen Ye, Yajie Gu, Ashley E. Sullivan, Yun Quan, Rebecca K. Lau, Huilin Zhou, Kevin D. Corbett, Aaron T. Whiteley
Summary: In humans, the immune receptor cGAS detects viral infection and activates antiviral signalling. Bacteria have a similar system called CD-NTases, which detect bacteriophage infection. The protein Cap2 primes bacterial CD-NTases for activation through a ubiquitin transferase-like mechanism, leading to increased production of cGAMP.
Article
Chemistry, Organic
Heyuan Lu, Kishor L. Handore, Tabitha E. Wood, Grace K. Shimokura, Aaron D. Schimmer, Robert A. Batey
Summary: The first total synthesis of the E1 ubiquitin-activating enzyme inhibitor, himeic acid A, is described. Key steps include the use of a McCombie reaction and a dioxenone ring-opening/acyl ketene trapping reaction to form the core structure, as well as the installation of the (S)-2-methyl succinic acid fragment and the unsaturated side-chain.
Article
Multidisciplinary Sciences
Lingmin Yuan, Zongyang Lv, Melanie J. Adams, Shaun K. Olsen
Summary: By determining the structure of a covalently trapped E1-E2-ubiquitin thioester mimetic, researchers identified two distinct conformations of the E1-E2-Ub complex which suggest the presence of an affinity switch mechanism facilitating thioester transfer.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Maki Sakuma, Piers Blombery, Manja Meggendorfer, Claudia Haferlach, Markus Lindauer, Uwe M. Martens, Wolfgang Kern, Torsten Haferlach, Wencke Walter
Summary: The study identified three somatic mutations in the UBA1 gene that are associated with the development of VEXAS syndrome. Additionally, the analysis revealed several putative somatic variants in UBA1 that may contribute to myeloid malignancies, particularly in patients with vacuoles or immunodysregulatory symptoms.
Article
Biochemistry & Molecular Biology
Emilie De Hert, An Bracke, Isabel Pintelon, Eline Janssens, Anne-Marie Lambeir, Pieter Van der Veken, Ingrid De Meester
Summary: This study investigates the C-terminal cleavage of (pyr)-apelin-13 in human endothelial cells, with PRCP responsible for this cleavage in human umbilical vein endothelial cells while ACE2 also contributes to it in aortic endothelial cells. Inflammatory stimulation induces PRCP secretion into the extracellular environment of endothelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Shuwei Wang, Qi Han, Zhaolan Wei, Yunyi Wang, Lingfu Deng, Mingqing Chen
Summary: The study found that exposure to formaldehyde can cause an increase in blood pressure and damage to the heart, aortic vessels, and kidneys. By blocking the ACE/AT1R axis using an ACE inhibitor, the increase in blood pressure and pathological changes caused by formaldehyde exposure can be alleviated. This result improves our understanding of the impact of formaldehyde exposure on the development of hypertension.
Article
Medicine, Research & Experimental
Shih-Yi Lee, Yueh-Hsiung Kuo, Chen-Xuan Du, Cheng-Wei Huang, Hui-Chun Ku
Summary: Differentiation of cardiac fibroblasts into myofibroblasts is a critical event in the progression of cardiac fibrosis. The study investigated the effect of caffeic acid ethanolamide (CAEA) on cardiac remodeling induced by Angiotensin (Ang) II. CAEA inhibited fibroblast differentiation, protected against cardiac fibrosis and dysfunction, and regulated the TGF-beta/SMAD/NOX4 signaling pathway. The study supports CAEA administration as a viable method to prevent Ang II-induced cardiac remodeling.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Pharmacology & Pharmacy
Yu-Kai Cui, Yi-Xiang Hong, Wei-Yin Wu, Wei-Min Han, Yao Wu, Chan Wu, Gui-Rong Li, Yan Wang
Summary: The study found that acacetin can reduce myocardial hypertrophy by activating the AMPK/PGC-1 alpha signaling pathway through Sirt1, which leads to a decrease in oxidation, inflammation, and apoptosis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Cell Biology
Meng-Qiu Dang, Xue-Chen Zhao, Song Lai, Xia Wang, Lei Wang, Yun-Long Zhang, Yang Liu, Xiao-Hong Yu, Ying Liu, Hui-Hua Li, Yun-Long Xia
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2015)
Article
Medicine, Research & Experimental
Wen-Jun Li, Ying Liu, Jing-Jing Wang, Yun-Long Zhang, Song Lai, Yun-Long Xia, Hong-Xia Wang, Hui-Hua Li
Article
Cardiac & Cardiovascular Systems
Hong-Xia Wang, Wen-Jun Li, Cui-Liu Hou, Song Lai, Yun-Long Zhang, Cui Tian, Hui Yang, Jie Du, Hui-Hua Li
CARDIOVASCULAR RESEARCH
(2019)
Article
Cell Biology
Shuang Liu, Li-Xin Liu, Yun-Long Zhang, Song Lai, Yun-Peng Xie, Nan-Nan Li, Hong-Xia Wang, Yun-Long Xia, Ying Liu, Hui-Hua Li
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Cell Biology
Yun-Long Zhang, Lian-Yun Zhi, Lei-Xin Zou, Chen Chen, Jie Bai, Qiu-Yue Lin, Song Lai, Lei Wang, Ying Liu, Hui-Hu Li
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Cell Biology
Jiming Yin, Yangjia Wang, Jing Chang, Bin Li, Jia Zhang, Yu Liu, Song Lai, Ying Jiang, Huihua Li, Xiangjun Zeng
CELL DEATH & DISEASE
(2018)
Article
Multidisciplinary Sciences
Xin Xie, Hai-Lian Bi, Song Lai, Yun-Long Zhang, Nan Li, Hua-Jun Cao, Ling Han, Hong-Xia Wang, Hui-Hua Li
Article
Medicine, Research & Experimental
Xin Huang, Yang Liu, Xiaolei Yang, Song Lai, Yunlong Zhang, Jie Gu, Huihua Li, Yunpeng Xie, Yunlong Xia
Article
Cell Biology
Xin Xie, Yi-Fan Tie, Song Lai, Yun-Long Zhang, Hui-Hua Li, Ying Liu
Summary: This study revealed the importance of CGI-58 in maintaining heart function, with CGI-58 knockout leading to cardiac hypertrophic remodeling and dysfunction, while CGI-58 injection alleviated these adverse effects. Proteomic analysis showed that ER stress plays a crucial role in cardiac hypertrophy and dysfunction.
CELL DEATH & DISEASE
(2021)
Correction
Medicine, Research & Experimental
Yun-Peng Xie, Song Lai, Qiu-Yue Lin, Xin Xie, Jia-Wei Liao, Hong-Xia Wang, Cui Tian, Hui-Hua Li
Correction
Cardiac & Cardiovascular Systems
Hong-Xia Wang, Wen-Jun Li, Cui-Liu Hou, Song Lai, Yun-Long Zhang, Cui Tian, Hui Yang, Jie Du, Hui-Hua Li
CARDIOVASCULAR RESEARCH
(2021)
Article
Surgery
Bo Sun, Fangda Li, Song Lai, Xu Zhang, Hongxia Wang, Yuan Li, Wei Wang, Yuexin Chen, Bao Liu, Yuehong Zheng
Summary: The study investigated the relationship between atherosclerotic abdominal aortic aneurysm (AAA) and CXC chemokine receptor type 2 (CXCR2) using a mouse model. Inhibition of CXCR2 was shown to reduce the size of AAA, inflammation, and phenotypic changes in blood vessels, suggesting that targeting CXCR2 may be a new treatment strategy for AAA.
ACTA CIRURGICA BRASILEIRA
(2021)
Article
Medicine, Research & Experimental
Yun-Peng Xie, Song Lai, Qiu-Yue Lin, Xin Xie, Jia-Wei Liao, Hong-Xia Wang, Cui Tian, Hui-Hua Li
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)