Article
Genetics & Heredity
Rod Carlo Agram Columbres, Yue Chin, Sanjana Pratti, Colin Quinn, Luis F. Gonzalez-Cuyar, Michael Weiss, Fabiola Quintero-Rivera, Virginia Kimonis
Summary: This study reports the clinical and genetic analysis findings in five patients with VCP gene mutations, including novel variants associated with MSP1 manifestations. The study highlights the heterogeneity in clinical symptoms and emphasizes the need for increased awareness and early consideration of MSP1 in the differential diagnosis of myopathies and/or PDB, dementia, or ALS to improve diagnosis and management.
Article
Genetics & Heredity
Yalan Wan, Qi Wang, Yiming Zheng, Meng Yu, Zhiying Xie, Chen Ling, Lingchao Meng, Jiaxi Yu, Yilei Zheng, Yikang Wang, Wenhao Zhang, Chang Liu, Yawen Zhao, Yun Yuan, Jianwen Deng, Qiang Gang, Zhaoxia Wang
Summary: The study aimed to investigate the clinical features, genetic characteristics, muscle imaging, and muscle pathological changes in Chinese patients with VCP gene mutations. Variants were identified using next-generation sequencing and confirmed with Sanger sequencing. Muscle MRIs showed the presence of isolated island sign or contra-isolated island sign in four out of five patients. Muscle biopsies revealed neuropathic and myopathic changes in seven patients and muscle dystrophic changes in two patients. Rimmed vacuoles and protein aggregates were observed in all patients. The findings expand the mutational spectrum of the VCP gene and provide valuable clues for genetic diagnosis.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2023)
Article
Genetics & Heredity
Alyaa Shmara, Mari Perez-Rosendahl, Kady Murphy, Ashley Kwon, Charles Smith, Virginia Kimonis
Summary: This study expands the phenotype of VCP disease to potentially include rare cancers and highlights the importance of further investigation of the role of VCP in cancer development. The results suggest that VCP disease patients may have an increased risk for rare tumors. A larger study is needed to determine if patients with VCP disease develop cancer at a higher rate than the general population. If that is the case, they should be followed up more frequently and screened for recurrence and metastasis of their cancer.
ORPHANET JOURNAL OF RARE DISEASES
(2022)
Article
Clinical Neurology
Anna Luzzi, Feng Wang, Shan Li, Michelina Iacovino, Tsui-Fen Chou
Summary: In this study, iPSCs were generated from fibroblasts of patients with the R155H/+ mutation and differentiated into myotubes. Proteomic analysis revealed dysregulated expression of proteins involved in various cellular functions, including skeletal muscle function, cell signaling, and cell adhesion. These findings provide molecular evidence and potential therapeutic targets for treating IBMPFD/ALS.
FRONTIERS IN NEUROLOGY
(2023)
Article
Cell Biology
Jordan M. Wall, Ankita Basu, Elizabeth R. M. Zunica, Olga S. Dubuisson, Kathryn Pergola, Joshua P. Broussard, John P. Kirwan, Christopher L. Axelrod, Alyssa E. Johnson
Summary: By using CRISPR/Cas9 technology, Drosophila knock-in mutants with nine hereditary VCP disease mutations have been generated, displaying characteristics of VCP-mediated degeneration and some novel findings, providing valuable insights for studying the etiology of VCP diseases and testing potential therapies.
DISEASE MODELS & MECHANISMS
(2021)
Review
Clinical Neurology
Manisha K. Korb, Virginia E. Kimonis, Tahseen Mozaffar
Summary: Multisystem proteinopathy (MSP) is a group of inherited disorders that cause neurodegeneration, myopathy, and bone disease, sharing common pathophysiology. In addition to mutations in the VCP gene, several other genes are responsible for similar clinical and pathological phenotypes affecting muscle, brain, nerve, and bone.
Review
Genetics & Heredity
Gerald Pfeffer, Grace Lee, Carly S. Pontifex, Roberto D. Fanganiello, Allison Peck, Conrad C. Weihl, Virginia Kimonis
Summary: This work reviews the clinical features and genetic diagnosis of diseases caused by mutations in the VCP gene. VCP is crucial for cellular functions and its mutations can lead to various neurodegenerative diseases and non-neurological symptoms.
Article
Cell Biology
Dragomir B. Krastev, Shudong Li, Yilun Sun, Andrew Wicks, Gwendoline Hoslett, Daniel Weekes, Luned M. Badder, Eleanor G. Knight, Rebecca Marlow, Mercedes Pardo Calvo, Lu Yu, Tanaji T. Talele, Jiri Bartek, Jyoti Choudhary, Yves Pommier, Stephen J. Pettitt, Andrew Tutt, Kristijan Ramadan, Christopher J. Lord
Summary: The study reveals the regulatory mechanism of trapped PARP1, which is undergoes SUMOylation and ubiquitylation, leading to the recruitment of p97 ATPase for the removal of trapped PARP1 from chromatin and prevention of PARP inhibitor-induced cytotoxicity. Inhibitors targeting p97-complex prolong the trapping of PARP1, enhancing the cytotoxicity of PARP inhibitors in certain types of tumor cells.
NATURE CELL BIOLOGY
(2022)
Editorial Material
Cell Biology
Lidia Wrobel, Sandra M. Hill, Avraham Ashkenazi, David C. Rubinsztein
Summary: VCP protein plays a role in promoting autophagosome biogenesis by regulating the levels of PtdIns3P, stabilizing autophagy-related proteins through interactions with ATXN3 and the PtdIns3K complex.
Article
Cell Biology
Chuang Li, Yongsheng Huang, Qianqian Fan, Hongyang Quan, Yeqing Dong, Meng Nie, Jiaqi Wang, Fucun Xie, Jiang Ji, Lan Zhou, Zhi Zheng, Lin Wang
Summary: p97, an ATPase commonly overexpressed in many cancers, is positively correlated with histological grade, tumor size, and lymph node metastasis in breast cancers. High levels of p97 are found in stem-like cancer cells and cancer stem cell populations, with p97 expression positively correlating with SOX2. Depletion or inhibition of p97 leads to reduced cancer growth and CSC population, primarily affecting ALDH(+) CSCs and CSC-enriched mammospheres.
CELL DEATH & DISEASE
(2021)
Article
Microbiology
Poulami Das, Wendy Kaichun Xu, Amit Kumar Singh Gautam, Mary M. Lozano, Jaquelin P. Dudley
Summary: The study of viral replication has provided important insights into host processes, and in this paper, the researchers focused on the mouse mammary tumor virus (MMTV). They discovered the critical role of a signal peptide (SP) in the virus's interaction with host cells and its nuclear trafficking, and identified specific amino acids that are important for these processes.
Article
Medicine, Research & Experimental
Cheng Cheng, Lan Weiss, Henri Leinonen, Alyaa Shmara, Hong Z. Yin, Timothy Ton, Annie Do, Jonathan Lee, Lac Ta, Eshanee Mohanty, Jesse Vargas, John Weiss, Krzysztof Palczewski, Virginia Kimonis
Summary: This study evaluated the safety and efficacy of a novel VCP inhibitor, CB-5083, in a mouse model of VCP disease. The results showed that CB-5083 can improve VCP disease-associated muscle pathology and is well tolerated. These findings suggest that VCP inhibitors could be beneficial in the treatment of VCP disease.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Qiong Yuan, Yunwei Sun, Fan Yang, Dan Yan, Meihua Shen, Zhigang Jin, Lin Zhan, Guangqi Liu, Ling Yang, Qianyi Zhou, Zhijun Yu, Xiangyu Zhou, Yang Yu, Yong Xu, Qingming Wu, Jianfang Luo, Xiamin Hu, Chunxiang Zhang
Summary: We found that DICAR, a conserved circRNA, was downregulated in diabetic mouse hearts. DICAR had an inhibitory effect on diabetic cardiomyopathy (DCM), and its deficiency led to cardiac dysfunction, hypertrophy, and fibrosis in mice. On the molecular level, DICAR-VCP-Med12 degradation could be the underlying mechanism for DICAR-mediated effects. The synthesized DICAR-JP showed similar effects, and the expression of DICAR in diabetic patients was lower than in healthy controls, suggesting that DICAR and DICAR-JP could be potential drug candidates for DCM.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Purbasha Nandi, Shan Li, Rod Carlo A. Columbres, Feng Wang, Dewight R. Williams, Yu-Ping Poh, Tsui-Fen Chou, Po-Lin Chiu
Summary: The study visualized the structures of p97(R155H) and p47 cofactor using single-particle cryo-EM, revealing that more than one-third of the population is in the dodecameric form. Presence of nucleotides dissociates the dodecamer into two hexamers, enhancing its function significantly. The N-domains of the p97(R155H) mutant display consistent configurations in ADP- or ATP gamma S-bound states.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Youngdae Gwon, Brian A. Maxwell, Regina-Maria Kolaitis, Peipei Zhang, Hong Joo Kim, J. Paul Taylor
Summary: Stress granules are dynamic condensates composed of RNA and protein that disassemble in a context-dependent manner, with ubiquitination of G3BP1 being required for disassembly in the setting of heat shock.
Article
Biochemistry & Molecular Biology
Grace Png, Raffaele Gerlini, Konstantinos Hatzikotoulas, Andrei Barysenka, N. William Rayner, Lucija Klaric, Birgit Rathkolb, Juan A. Aguilar-Pimentel, Jan Rozman, Helmut Fuchs, Valerie Gailus-Durner, Emmanouil Tsafantakis, Maria Karaleftheri, George Dedoussis, Claus Pietrzik, James F. Wilson, Martin Hrabe Angelis, Christoph Becker-Pauly, Arthur Gilly, Eleftheria Zeggini
Summary: In this study, a protein quantitative trait locus (pQTL) analysis was performed on two Greek cohorts, identifying 301 independently associated pQTL variants for 170 proteins related to cardiometabolic processes. The study also discovered rare variants and observed changes in frequency of certain pQTL variants in the isolated populations. Additionally, the study identified proteins causally associated with cardiometabolic traits and described a knock-out Mep1b mouse model.
HUMAN MOLECULAR GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Lillian Garrett, Dietrich Truembach, Nadine Spielmann, Wolfgang Wurst, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabe De Angelis, Sabine M. Hoelter
Summary: Neuropsychiatric diseases represent a significant global disease burden and require innovative approaches for pathogenic understanding, biomarker identification, and therapeutic strategies. The malfunction of the heart/brain axis, particularly through the autonomic nervous system and brain central autonomic network interaction, plays a crucial role in the etiology of these diseases. This inter-relationship offers potential avenues for novel diagnosis and treatment approaches.
Article
Biochemistry & Molecular Biology
Nadine Spielmann, Christina Schenkl, Timea Komlodi, Patricia da Silva-Buttkus, Estelle Heyne, Jana Rohde, Oana Amarie, Birgit Rathkolb, Erich Gnaiger, Torsten Doenst, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabe de Angelis, Marten Szibor
Summary: Deletion of the Uqcrh gene causes abnormalities in cardiac morphology and contractility in mice, but does not increase the production of mitochondrial reactive oxygen species. This study provides important information for understanding rare mitochondrial disorders.
Article
Multidisciplinary Sciences
Rakesh Ganji, Joao A. Paulo, Yuecheng Xi, Ian Kline, Jiang Zhu, Christoph S. Clemen, Conrad C. Weihl, John G. Purdy, Steve P. Gygi, Malavika Raman
Summary: The association between the endoplasmic reticulum (ER) and mitochondrial membranes at ER-Mitochondria contact sites (ERMCS) is crucial for lipid synthesis. However, little is known about how these contacts are remodeled and the impact of altered contacts on lipid metabolism. This study reveals that the p97 AAA-ATPase and its adaptor UBXD8 play a role in regulating ERMCS. Loss of p97-UBXD8 leads to increased contacts and changes in lipid metabolism, including membrane lipid saturation. The SREBP1-SCD1 pathway is negatively affected, suggesting the sensitivity of contacts to alterations in membrane lipid composition and saturation.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Andreas Unger, Andreas Roos, Andrea Gangfuss, Andreas Hentschel, Dieter Glaeser, Karsten Krause, Kristina Doering, Ulrike Schara-Schmidt, Sabine Hoffjan, Matthias Vorgerd, Anne-Katrin Guettsches
Summary: This study presents new insights into the molecular characteristics of BICD2-associated disorders, especially in relation to muscle pathologies. Clinical and imaging features showed a typical pattern of distal lower extremity paresis. Histopathological and proteomic analysis revealed muscle cell vulnerability and abnormal levels of thrombospondin-4 and biglycan.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Dominik Sellung, Lorena Heil, Nassam Daya, Frank Jacobsen, Janine Mertens-Rill, Heidi Zhuge, Kristina Doering, Misagh Piran, Hendrik Milting, Andreas Unger, Wolfgang A. Linke, Rudi Kley, Corinna Preusse, Andreas Roos, Dieter O. Fuerst, Peter F. M. van der Ven, Matthias Vorgerd
Summary: Myofibrillar myopathies (MFM) are chronic muscle diseases characterized by protein aggregate accumulation and muscle fiber failure. Heterozygous mutations in the filamin C (FLNc) gene cause a subtype of MFM with muscle weakness, structural alterations, and protein accumulation. This study characterizes two novel truncating FLNc variants and their impact on FLNc stability, distribution, and protein quality system (PQS) pathways. The variants result in myopathy with intracellular protein aggregates and different PQS alterations. Further studies are needed to confirm these findings.
Article
Cell Biology
Marlena Rohm, Leon Volke, Lara Schlaffke, Robert Rehmann, Nicolina Suedkamp, Andreas Roos, Anne Schaenzer, Andreas Hentschel, Matthias Vorgerd
Summary: This study investigates early proteomic changes in a mouse model of Pompe disease and identifies potential therapeutic pathways. Proteomic analysis reveals 538 significantly upregulated and 16 significantly downregulated proteins in the quadriceps muscles of Pompe mice compared to wildtype mice. This study highlights the importance of early diagnosis and treatment of Pompe disease and suggests potential therapeutic strategies.
Article
Cell Biology
Lennart Norman Stegemann, Paula Maria Neufeld, Ines Hecking, Matthias Vorgerd, Veronika Matschke, Sarah Stahlke, Carsten Theiss
Summary: The enteric nervous system (ENS) is an intrinsic network of neuronal ganglia in the intestine with a large number of neurons. Understanding the neuroprotective effects of progesterone in the ENS is important because these neurons are affected in neurodegenerative diseases. The study showed the expression of different progesterone receptors in rats at different developmental stages and demonstrated the potential neuroprotective effects of progesterone in the ENS using cultured neurons.
Article
Cell Biology
Lilli Winter, Ilona Staszewska-Daca, Stefan Zittrich, Fatiha Elhamine, Michaela M. Zrelski, Katy Schmidt, Irmgard Fischer, Christian Juengst, Astrid Schauss, Wolfgang H. Goldmann, Robert Stehle, Gerhard Wiche
Summary: Plectin is an important protein for maintaining the integrity and function of myofibers. It is mainly expressed in skeletal muscle and the heart, and its loss can lead to muscle weakness and structural disorganization. Plectin is located between Z-disks and interacts with Desmin intermediate filaments. Its isoform P1d plays a role in tension-induced proteolysis during high loads of physical exercise and muscle contraction.
Article
Cell Biology
Daniel Garger, Martin Meinel, Tamina Dietl, Christina Hillig, Natalie Garzorz-Stark, Kilian Eyerich, Martin Hrabe de Angelis, Stefanie Eyerich, Michael P. P. Menden
Summary: Through studying the association between various phenotypic traits and lifespan, it was found that somatic mutation and resting heart rate are negatively correlated with lifespan, while other traits show strong associations. Resting heart rate enhances the prediction of lifespan, indicating its direct influence or representation of lower-level mechanisms associated with lifespan.
Article
Genetics & Heredity
Daniel G. Calame, Tianyu Guo, Chen Wang, Lillian Garrett, Angad Jolly, Moez Dawood, Alina Kurolap, Noa Zunz Henig, Jawid M. Fatih, Isabella Herman, Haowei Du, Tadahiro Mitani, Lore Becker, Birgit Rathkolb, Raffaele Gerlini, Claudia Seisenberger, Susan Marschall, Jill Hunter, Amanda Gerard, Alexis Heidlebaugh, Thomas Challman, Rebecca C. Spillmann, Shalini N. Jhangiani, Zeynep Coban-Akdemir, Seema Lalani, Lingxiao Liu, Anya Revah-Politi, Alejandro Iglesias, Edwin Guzman, Evan Baugh, Nathalie Boddaert, Sophie Rondeau, Clothide Ormieres, Giulia Barcia, Queenie K. G. Tan, Sophie Isabelle Thiffault, Tomi Pastinen, Kazim Sheikh, Suur Biliciler, Davide Mei, Federico Melani, Vandana Shashi, Yuval Yaron, Mary Steele, Emma Wakeling, Elsebet Ostergaard, Francisca Undiagnosed Dis Network, Francisca Millan, Teresa Santiago-Sim, Julien Thevenon, Ange-Line Bruel, Christel Thauvin-Robinet, Denny Popp, Konrad Platzer, Pawel Gawlinski, Wojciech Wiszniewski, Dana Marafi, Davut Pehlivan, Jennifer E. Posey, Richard A. Gibbs, Valerie Gailus-Durner, Renzo Guerrini, Helmut Fuchs, Martin Hrabe de Angelis, Sabine M. Hoelter, Hoi-Hung Cheung, Shen Gu, James R. Lupski
Summary: DExD/H-box RNA helicases (DDX/DHX) are part of a large gene family that encodes enzymes and variations in these genes can lead to neurodevelopmental disorders and cancer. By analyzing genetic data, researchers found rare variants in the DHX9 gene in individuals with different disorders ranging from neurodevelopmental disorders to a specific type of polyneuropathy. Further experiments confirmed that DHX9 is important for neurodevelopment and neuronal homeostasis.
AMERICAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Cell Biology
Jennifer Maurer, Xinjie Zhao, Martin Irmler, Anders Gudiksen, Nanna S. Pilmark, Qi Li, Thomas Goj, Johannes Beckers, Martin Hrabe de Angelis, Andreas L. Birkenfeld, Andreas Peter, Rainer Lehmann, Henriette Pilegaard, Kristian Karstoft, Guowang Xu, Cora Weigert
Summary: Metformin treatment leads to lactate production and secretion, as well as reduced glucose consumption. It inhibits respiratory chain complex I and alters cellular redox state, decreasing pyruvate oxidation. These findings suggest that metformin induces dose-dependent lactate production in skeletal muscle by shifting the equilibrium of lactate dehydrogenase reaction.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Correction
Cell Biology
Lilli Winter, Ilona Staszewska-Daca, Stefan Zittrich, Fatiha Elhamine, Michaela M. Zrelski, Katy Schmidt, Irmgard Fischer, Christian Juengst, Astrid Schauss, Wolfgang H. Goldmann, Robert Stehle, Gerhard Wiche
Meeting Abstract
Endocrinology & Metabolism
R. Jumpertz von Schwartzenberg, K. Bodis, J. Martin, M. Schon, M. Hrabe de Angelis, N. Perakakis, S. Kabisch, A. Pfeiffer, M. Blueher, J. Szendroedi, N. Stefan, R. Wagner, A. L. Birkenfeld, M. Roden, A. Fritsche
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)