Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 443, Issue 4, Pages 1245-1250Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2013.12.123
Keywords
3T3-L1 cells; Adipogenesis; Real-time analysis; Impedance
Categories
Funding
- National Research Foundation (NRF), Department of Science and Technology (DST)
- Medical Research Council (MRC)
- Kresge Foundation and Rhodes University
- NRF Scarce Skills Scholarship
- MRC Scholarship
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Real-time analysis offers multiple benefits over traditional end point assays. Here, we present a method of monitoring the optimisation of the growth and differentiation of murine 3T3-L1 preadipocytes to adipocytes using the commercially available ACEA xCELLigence Real-Time Cell Analyser Single Plate (RTCA SP) system. Our findings indicate that the ACEA xCELLigence RTCA SP can reproducibly monitor the primary morphological changes in pre- and post-confluent 3T3-L1 fibroblasts induced to differentiate using insulin, dexamethasone, 3-isobutyl-1-methylxanthine and rosiglitazone; and may be a viable primary method of screening compounds for adipogenic factors. (C) 2013 Elsevier Inc. All rights reserved.
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