Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 447, Issue 1, Pages 51-56Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.03.104
Keywords
SREBP-1; SGBS cells; Proliferation; Senescence
Categories
Funding
- Spanish Ministry of Education and Science [SAF2006-06760, SAF2012-39732]
- Consejo Superior de Investigaciones Cientificas (JAE-predoc)
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Sterol regulatory element binding proteins (SREBP), encoded by the Srebf1 and Srebf2 genes, are important regulators of genes involved in cholesterol and fatty acid metabolism. Whereas SREBP-2 controls the cholesterol synthesis, SREBP-1 proteins (-1a and -1c)function as the central hubs in lipid metabolism. Despite the key function of these transcription factors to promote adipocyte differentiation, the roles of SREBP-1 proteins during the preadipocyte state remain unknown. Here, we evaluate the role of SREBP-1 in. preadipocyte proliferation using RNA interference technology. Knockdown of the SREBP-1 a gene decreased the proliferation rate in human SGBS preadipocyte cell strain without inducing senescence. Furthermore, our data identified retinoblastoma binding protein 8 and cyclin-dependent kinase inhibitor 3 genes as new potential SREBP-1 targets, in addition to cyclin-dependent kinase inhibitor 1A which had already been described as a gene regulated by SREBP-la. These data suggested a new role of SREBP-1 in adipogenesis via regulation of preadipocyte proliferation. (C) 2014 Elsevier Inc. All rights reserved.
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