Review
Pharmacology & Pharmacy
Toshio Kanno, Takahiro Nakajima, Keisuke Miyako, Yusuke Endo
Summary: Th17 cells play a crucial role in the pathogenesis of autoimmune disorders, and their differentiation is regulated by lipid metabolism and the activation of ROR?t. Inhibition of lipid biosynthesis suppresses Th17 cell differentiation. Manipulating cellular lipid metabolism provides a novel therapeutic approach for treating autoimmune disorders.
PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Cell Biology
Riping Xiao, Kawai Lei, Hioha Kuok, Wende Deng, Yuxin Zhuang, Yanqing Tang, Zhengyang Guo, Hongyan Qin, Li-Ping Bai, Ting Li
Summary: Primary bile acids (BAs), byproducts of cholesterol metabolism, are closely associated with the pathogenesis of cholestatic liver diseases. In this study, a synthesized sulfated product of lithocholic acid (LCA) was found to selectively inhibit the differentiation of Th17 cells, without affecting other types of T cells. This finding provides clues for further exploration of the relationship between bile acid metabolites and T lymphocytes.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Manman Wang, Tiantian Su, Hanfei Sun, Huijuan Cheng, Chunru Jiang, Paipai Guo, Zhenduo Zhu, Ruhong Fang, Feng He, Mingli Ge, Qiuyun Guan, Wei Wei, Qingtong Wang
Summary: This study investigated the therapeutic effect and primary pharmacological mechanism of Ziyuglycoside I on collagen-induced arthritis mice. The results showed that Ziyuglycoside I effectively improved arthritis symptoms by inhibiting mTOR activation and regulating Th17/Treg balance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xuyan Tian, Lipeng Tang, Fengjiao Wei, Huanpeng Chen, Longxiang Sheng, Yuying Yang, Xiaoqing Zhou, Yongchao Li, Xiaoting Xu, Boyang Zhang, Zhonghua Liu, Yu Lei, Bolan Yu, Chuan Bai, Xixin He, Zhaofeng Huang
Summary: The study demonstrates the potent therapeutic effects of three pentacyclic triterpene compounds isolated from loquat leaves on psoriasis, alleviating skin inflammation, abnormal keratinocyte proliferation, and immune cell infiltration. These compounds show promising potential for drug development in treating psoriasis with better performance than steroids.
MOLECULAR IMMUNOLOGY
(2021)
Article
Integrative & Complementary Medicine
Di Hua, Jie Yang, Qinghai Meng, Yuanyuan Ling, Qin Wei, Zhigang Wang, Qingyun Wei, Jiao Chen, Juan Ye, Xuan Han, Kelei Su, Weikang Kong, Chao Xu, Peng Cao, Chunping Hu
Summary: This study demonstrates that the Soufeng sanjie formula (SF) has anti-arthritic effects by reducing arthritis symptoms, inflammation levels, and restoring Th17/Treg cell balance in collagen-induced arthritis (CIA) mice.
Article
Biology
Tsan-Tzu Yang, Ming-Feng Chiang, Che-Chang Chang, Shii-Yi Yang, Shih-Wen Huang, Nan-Shih Liao, Hsiu-Ming Shih, Wei Hsu, Kuo- Lin
Summary: SENP2 is involved in regulating post-translational modification of Smad4 in Th17 cells and intestinal bowel diseases. Lack of Senp2 exacerbates the severity of experimental colitis and leads to elevated levels of pathogenic Th17 cells and dysbiosis of the intestinal microbiome. SENP2 enzymatic activity is important for deSUMOylation of Smad4 and regulates the pathogenicity of Th17 cells.
COMMUNICATIONS BIOLOGY
(2023)
Article
Immunology
Tao Wang, Wenjing Feng, Mengwei Ju, Huiyan Yu, Zhiting Guo, Xuejing Sun, Kexin Yang, Miao Liu, Rong Xiao
Summary: Our study found that there was an enhanced level of 27-OHC in the serum of individuals with mild cognitive impairment (MCI). The balance of Th17-related cytokines was altered, with increased levels of IL-17A, IL-12p70, IL-23, GM-CSF, MIP-3 alpha, and TNF-alpha, but decreased levels of IL-13, IL-28A, and TGF-beta 1. Further experiments showed that 27-OHC induced higher immunogenicity, leading to an increase in Th17 cells and a decrease in Treg cells. The amyloidosis was aggravated, cognitive deficits occurred, and the learning and memory performance were worsened in mice treated with 27-OHC. However, by inhibiting ROR gamma t in 27-OHC-loaded mice, the balance of Th17/Treg cells was restored, the amyloidosis was decreased, and the learning and memory decline was improved.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Biochemistry & Molecular Biology
Kai-Long Ji, Yan-Qing Tang, Zheng-Yang Guo, Pian-Chou GongPan, Peng Sun, Jian-Mei Lu, Meng-Yuan Dai, Ting Li
Summary: Chemical investigation of Glycosmis lucida Wall. ex C. C. Huang leaves led to the isolation of ten compounds, including two previously unreported geranylated sulfur-containing amides (1 and 2) and eight known compounds (3-10). Structural characterization was conducted using NMR, MS, and CD spectroscopic methods. Evaluation of the inhibitory effects of the isolates on Th17 differentiation revealed that compounds 1 and 6 significantly inhibited Th17 differentiation, with IC50 values of 0.36 and 1.30 μM, respectively. However, both compounds 1 and 6 failed to bind to retinoic acid-related orphan receptor gamma t (RORγt), indicating that their inhibition of Th17 differentiation is independent of RORγt.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Immunology
Jing Zhang, Ronald J. Bouch, Maxim G. Blekhman, Zhiheng He
Summary: Th17 cells play an important role in autoimmune pathology and exhibit plasticity in their pathogenic potential. Research has identified that ROR gamma t and the ubiquitination pathway play key roles in the pathogenesis of Th17 cells. USP19 suppresses the pathogenicity of Th17 cells by regulating the ubiquitin chain of ROR gamma t.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Chemistry, Medicinal
Ahmed Fouda, Sarita Negi, Oleg Zaremba, Rita S. Gaidar, Yurii S. Moroz, Eduard Rusanov, Steven Paraskevas, Jean Tchervenkov
Summary: This study discovered 2,3 derivatives of 4,5,6,7-tetrahydro-benzothiophene as potential modulators of RORγt and evaluated their interactions with the protein. The approach used in this study can be applied to discover modulators of nuclear receptors and other globular protein targets.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Immunology
Wen-Hui Qi, Yan-Yan Zhang, Kun Xing, Dong-Xia Hao, Fei Zhang, Rui-Ning Wang, Ming-Yue Bao, Meng-Yuan Tian, Ya-Na Yang, Xing Li, Yuan Zhang
Summary: Using structure-based virtual screening, this study identified a compound called DDC, which is abundant in plants and food, as a potential immunomodulator. DDC inhibits the activity of ROR gamma t, a transcription factor involved in Th17 cell differentiation, thus regulating Th17/Treg balance and showing promise in the treatment of inflammatory diseases like multiple sclerosis, inflammatory bowel disease, and organ transplant rejection.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Immunology
Luis Eduardo Alves Damasceno, Thiago Mattar Cunha, Fernando Queiroz Cunha, Tim Sparwasser, Jose Carlos Alves-Filho
Summary: The STING signaling pathway is important for regulating immune responses and has potential clinical applications in the treatment of chronic inflammation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ankitha Shetty, Santosh D. Bhosale, Subhash Kumar Tripathi, Tanja Buchacher, Rahul Biradar, Omid Rasool, Robert Moulder, Sanjeev Galande, Riitta Lahesmaa
Summary: Dysregulated function of Th17 cells is associated with immunodeficiencies and autoimmune disorders. FOSL1 and FOSL2 modulate the effector functions of Th17 cells, with new mechanistic links discovered through the first interactomes study in human Th17 cells.
Article
Biochemistry & Molecular Biology
Timothy S. Strutzenberg, Yingmin Zhu, Scott J. Novick, Ruben D. Garcia-Ordonez, Christelle Doebelin, Yuanjun He, Mi Ra Chang, Theodore M. Kamenecka, Dean P. Edwards, Patrick R. Griffin
Summary: ROR gamma, a ligand-dependent transcription factor, plays key roles in metabolic activity, immune function, and cancer progression. Structural studies show that ROR gamma elongates upon DNA recognition, preventing interdomain crosstalk. Coregulatory protein may be able to sense the ligand- and DNA-bound status of ROR gamma, influencing coregulator recruitment. This research sheds light on the regulation of orphan nuclear receptor target genes through DNA-dependent conformational changes.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Immunology
Jingyi Li, Jingwei Zhao, Longmin Chen, Hongling Gao, Jing Zhang, Danlei Wang, Yuan Zou, Qixiong Qin, Yi Qu, Jiangting Li, Yongjie Xiong, Zhe Min, Manli Yan, Zhijuan Mao, Zheng Xue
Summary: Parkinson's disease (PD) is characterized by loss of dopaminergic neurons and accumulation of alpha-Synuclein (alpha-Syn). Th17 cells and dysfunctional regulatory T cells (Tregs) are associated with the loss of dopaminergic neurons in PD. The mechanism underlying the Th17/Treg imbalance remains unknown.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)