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Endocrinology & Metabolism
Viviane S. da Silva, Jussara J. Simao, Victor Plata, Andressa Franca de Sousa, Roberta D. C. da Cunha de Sa, Cleiton Fagundes Machado, Taiza Stumpp, Maria Isabel C. Alonso-Vale, Lucia Armelin-Correa
Summary: This study analyzed the effects of a high-fat diet on the posttranscriptional modifications of H3K27 and the expression of histone-modifying enzymes in adipose-derived stromal cells (ASCs) from white adipose tissue. The results showed that an HFD significantly reduces the acetylation of H3K27 in ASCs and the expression of ATP-citrate lyase in subcutaneous ASCs.
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Oncology
Li-Xia Peng, Ming-Dian Wang, Ping Xie, Jun-Ping Yang, Rui Sun, Li-Sheng Zheng, Yan Mei, Dong-Fang Meng, Xing-Si Peng, Yan-Hong Lang, Yuan-Yuan Qiang, Chang-Zhi Li, Liang Xu, Zhi-Jie Liu, Ling-Ling Guo, De-Huan Xie, Di-Tian Shu, Si-Ting Lin, Fei-Fei Luo, Bi-Jun Huang, Chao-Nan Qian
Summary: Nasopharyngeal carcinoma (NPC) originates in the nasopharyngeal epithelium and is known for its high metastatic rate. The study found elevated expression of LACTB in tumor tissues of NPC, which correlated with poorer patient survival. The role of LACTB in promoting NPC metastasis is dependent on the activation of ERBB3/EGFR-ERK signaling pathway.
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Biochemistry & Molecular Biology
Zhiming Li, Xinzong Zhang, Shiming Xie, Xingping Liu, Caifeng Fei, Xunbin Huang, Yunge Tang, Li-quan Zhou
Summary: This study reveals the important role of Nuclear receptor binding SET domain protein 2 (Nsd2) in spermatogenesis. The loss of Nsd2 leads to apoptosis and abnormal development of spermatogenic cells, as well as dysregulation of genes and histone modifications. Furthermore, Nsd2 deficiency results in the retention of histones and increased protamine proteins in spermatozoa. These findings uncover the involvement of Nsd2 in chromatin remodeling during spermatogenesis and provide insights into the molecular mechanisms of epigenetic abnormalities affecting male reproductive health.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Peng Liu, Si-Jia Sun, Ying-Jie Ai, Xu Feng, Yi-Min Zheng, Yun Gao, Jin-Ye Zhang, Lei Zhang, Yi-Ping Sun, Yue Xiong, Miao Lin, Hai-Xin Yuan
Summary: This study comprehensively analyzed glycolysis and gluconeogenesis genes and identified triosephosphate isomerase 1 (TPI1) as significantly correlated with poor prognosis in lung adenocarcinoma (LUAD). Further experiments revealed that the oncogenic function of TPI1 depends on its translocation to cell nucleus.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Fang Zhang, Mert Icyuz, Andrzej Bartke, Liou Y. Sun
Summary: The study showed that GH intervention during early life significantly altered histone H3 markers in Ames dwarf mice, resulting in tissue-specific changes in H3 lysine methylation and acetylation. These findings indicate an important epigenetic mechanism by which hormonal signals during early life can influence aging and longevity in mammals.
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Biochemistry & Molecular Biology
Yao Wang, Sabine Groeger, Jiawen Yong, Sabine Ruf
Summary: Orthodontic tooth movement is a complex process involving inflammation and immune responses. Macrophages, as mechanically sensitive immune cells, may be activated by orthodontic force and play a role in orthodontic root resorption. The activation of macrophages is associated with H3 histone acetylation and M2 polarization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nofar Harpaz, Tamir Mittelman, Olga Beresh, Ofir Griess, Noa Furth, Tomer-Meir Salame, Roni Oren, Liat Fellus-Alyagor, Alon Harmelin, Sanda Alexandrescu, Joana Graca Marques, Mariella G. Filbin, Guy Ron, Efrat Shema
Summary: This study adapted CyTOF to analyze histone modifications in DIPG tumor cells and identified two epigenetically distinct subpopulations. These subpopulations were consistent across different tumor lines, and showed differences in proliferation capacity and expression of stem cell and differentiation markers. The study also demonstrated potential interactions between histone modifications and epigenetic alterations during the cell cycle.
Article
Biology
Emily Hsu, Nathan R. Zemke, Arnold J. Berk
Summary: The regulation of transcriptional elongation through promoter region histone H3 acetylation, especially by recruitment of the super-elongation complex dependent on CBP/p300, is an important mechanism that applies to about 7% of expressed protein-coding genes in human airway epithelial cells. The maintenance of promoter H3K18/27ac, but not enhancer H3K18/27ac, in response to inhibition of CBP/p300 acetyl transferase activity reveals a homeostatic mechanism that contributes to regulating gene expression. Additionally, the association of BRD4 at enhancers plays a role in controlling the release of paused Pol2 at nearby promoters.
Article
Multidisciplinary Sciences
Nazar Mashtalir, Hai T. Dao, Akshay Sankar, Hengyuan Liu, Aaron J. Corin, John D. Bagert, Eva J. Ge, Andrew R. D'Avino, Martin Filipovski, Brittany C. Michel, Geoffrey P. Dann, Tom W. Muir, Cigall Kadoch
Summary: This study identified the effects of chromatin features on mSWI/SNF activities and interactions, as well as the combinatorial contributions of complex module components, reader domains, and nucleosome engagement properties to the localization of complexes.
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Nutrition & Dietetics
Smita S. Ghare, Benjamin T. Charpentier, Dushan T. Ghooray, Jingwen Zhang, Manicka V. Vadhanam, Sreelatha Reddy, Swati Joshi-Barve, Craig J. McClain, Shirish S. Barve
Summary: This study investigated the role of protein acetylation in regulating hepatic CCL2 expression in alcohol-associated liver disease (ALD). The results showed that ethanol increased the acetylation of histone-H3, leading to increased CCL2 expression and subsequent inflammation and injury. Therapeutic modulation of the acetylation state by tributyrin prevented the acetylation changes and downregulated CCL2 expression, resulting in reduced inflammation and injury in the liver.
News Item
Biochemistry & Molecular Biology
Kanishk Jain, Brian D. Strahl
Summary: High-throughput biochemical and biological analyses of disease-associated histone mutations reveal key residues in globular cores that affect chromatin remodeling, nucleosome stability, and stem cell pluripotency.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Cell Biology
Fabien Moretto, N. Ezgi Wood, Minghao Chia, Cai Li, Nicholas M. Luscombe, Folkert J. van Werven
Summary: In this study, it is found that the transcription levels of the noncoding region IRT2 in S. cerevisiae can regulate opposing chromatin and transcription states, thus affecting the expression of the IME1 promoter and meiotic entry. The findings illustrate how intergenic transcription is crucial in controlling local chromatin state, gene expression, and cell fate outcomes.
Article
Biochemistry & Molecular Biology
Jie Gao, Wei Liu, Jiaqi Liu, Niping Hao, Jing Pei, Lifeng Zhang
Summary: This study used an aluminum-treated rat model and found that aluminum exposure impaired learning and memory abilities, and caused ultrastructural changes in hippocampal synapses and neurons. Aluminum treatment also affected the levels and activities of histone modifications and related enzymes.
NEUROCHEMICAL RESEARCH
(2023)
Review
Plant Sciences
Jun-Li Wang, Dong-Wei Di, Pan Luo, Li Zhang, Xiao-Feng Li, Guang-Qin Guo, Lei Wu
Summary: This review focuses on the molecular mechanisms through which epigenetic modifications regulate auxin biosynthesis, demonstrating that complex signaling pathways affect gene expression and subsequently protein production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yang Xu, Yameng Wang, Lulu Wang, Wenxing Liang, Qianqian Yang
Summary: Botrytis cinerea causes gray mold and leads to significant financial losses. Fungicide resistance of B. cinerea is a major concern in food safety and agricultural environmental protection. Sodium valproate (SV) has shown potential as a safe and effective antifungal agent by enhancing the acetylation of histone H3 and modifying gene expression to inhibit B. cinerea.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)