Article
Plant Sciences
Xun Liao, Xiao-Jie Li, Guan-Tao Zheng, Feng-Rui Chang, Lin Fang, Hang Yu, Jun Huang, Ya-Feng Zhang
Summary: Mitochondrion-encoded circRNAs are widespread and translatable in plants, and a newly developed computational pipeline efficiently identifies them, demonstrating their prevalence and translation in plants.
Article
Plant Sciences
Lisa Jung, Sarah Schleicher, Fatema Alsaied Taha, Mizuki Takenaka, Stefan Binder
Summary: The Arabidopsis thaliana genome contains over 450 nuclear genes encoding PPR proteins, many of which have unknown functions. Knockout of the At4g19440 gene, which codes for a P-type PPR protein, affects embryo development and seed maturation in Arabidopsis. Knockout of At4g19440 also leads to changes in the abundance of several mitochondria-encoded transcripts.
Article
Biochemistry & Molecular Biology
Sarah Schleicher, Stefan Binder
Summary: The study reveals the important roles of RNA processing factors 1 and 8 in Arabidopsis mitochondria, as well as the contribution of other factors to the formation of dicistronic transcripts.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Li Zhang, Jingli Chen, Liqun Zhang, Ying Wei, Yajuan Li, Xinyun Xu, Hui Wu, Zhong-Nan Yang, Jirong Huang, Fenhong Hu, Weihua Huang, Yong-Lan Cui
Summary: The plastid-localized PPR protein EMB1270 is essential for chloroplast biogenesis in Arabidopsis thaliana, playing a crucial role in the accumulation of photosynthetic proteins and splicing efficiency of specific plastid introns. EMB1270 interacts with CFM2 to facilitate the splicing of group II introns, highlighting its importance in coordinating chloroplast and nuclear gene expression.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yang Yang, Yan Zhao, Yingqi Zhang, Lihua Niu, Wanyue Li, Wenqin Lu, Jinfang Li, Patrick Schaefer, Yuling Meng, Weixing Shan
Summary: This study identifies the immune function of the AtRTP7 gene in Arabidopsis and reveals its role in RNA splicing of mitochondrial respiratory chain Complex I subunits. The study also finds that rtp7 plants exhibit enhanced resistance to Phytophthora parasitica, which might be due to elevated mitochondrial reactive oxygen species burst.
Article
Biochemical Research Methods
Tshegofatso Dikobe, Katlego Sehlabane, Enetia Bobo, Angela Sibanda-Makuvise, Patience Chatukuta, David Kawadza, Oziniel Ruzvidzo
Summary: Downstream signalling in plants is important for cell division, growth, and stress response. A new molecule, AtPPR, was identified with both adenylyl cyclase (AC) and kinase activities, suggesting cross-talking interactions between these two domains. This discovery has implications for improving plants, particularly agricultural crops.
PLANT MOLECULAR BIOLOGY REPORTER
(2023)
Article
Biology
Kurataka Otsuka, Akihito Mamiya, Mineko Konishi, Mamoru Nozaki, Atsuko Kinoshita, Hiroaki Tamaki, Masaki Arita, Masato Saito, Kayoko Yamamoto, Takushi Hachiya, Ko Noguchi, Takashi Ueda, Yusuke Yagi, Takehito Kobayashi, Takahiro Nakamura, Yasushi Sato, Takashi Hirayama, Munetaka Sugiyama
Summary: The study identified the importance of mitochondrial RNA processing in regulating cell division during organogenesis in plants. Mutants with defects in mitochondrial RNA processing formed abnormally broadened lateral roots under high-temperature conditions due to extra cell division. The findings suggest that reactive oxygen species generated by defective mitochondrial respiration trigger the fasciation phenotype in these mutants.
Article
Plant Sciences
Hiroshi Yamamoto, Nozomi Sato, Toshiharu Shikanai
Summary: The chloroplast NADH dehydrogenase-like (NDH) complex plays a crucial role in the electron transport chain in plants. The assembly and stability of subcomplex A (SubA) is dependent on the biosynthesis of the NdhA protein. Knockout mutants of ndhA in tobacco plants revealed the essential role of NdhA in stabilizing specific subunits of the NDH complex.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ruchika, Toshifumi Tsukahara
Summary: This study investigated U-to-C RNA editing in Arabidopsis tissues and its effects on mRNA stability, focusing on PPR proteins. The research showed that U-to-C RNA editing affects the stability of edited genes' mRNA and identified such editing in the nuclear PPR gene of 12-day-old seedlings. Additionally, the study explored the impact of U-to-C RNA editing on mRNA structure and stability using a transcription inhibitor.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Noam Shahar, Tamar Elman, Rosalind Williams-Carrier, Oren Ben-Zvi, Iftach Yacoby, Alice Barkan
Summary: The genomes of microalgal chloroplasts have advantages for expressing foreign proteins, but efficient systems are needed to regulate their expression. Studies show that nucleus-encoded chloroplast gene activators from land plants can activate chloroplast transgenes in microalgae. However, limited activation may be due to suboptimal expression of the nuclear transgenes.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Cell Biology
Yusuke Yagi, Takamasa Teramoto, Shuji Kaieda, Takayoshi Imai, Tadamasa Sasaki, Maiko Yagi, Nana Maekawa, Takahiro Nakamura
Summary: This article introduces a protein-based RNA manipulation technology using proteins containing PPR motifs. By developing a rapid construction and evaluation method, researchers successfully constructed dozens of functional designer PPR proteins that can target specific 18 nt-long RNA molecules and control cellular functionality.
Article
Biochemistry & Molecular Biology
Elena Lesch, Maximilian T. Schilling, Sarah Brenner, Yingying Yang, Oliver J. Gruss, Volker Knoop, Mareike Schallenberg-Ruedinger
Summary: RNA editing processes differ significantly between animals and plants, with plant PPR proteins playing a key role in plant organelle cytidine-to-uridine editing. It has been discovered that plant PPR editing factors can operate in the nucleo-cytosolic environment of humans and be re-engineered towards new targets.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biology
Santana Royan, Bernard Gutmann, Catherine Colas des Francs-Small, Suvi Honkanen, Jason Schmidberger, Ashley Soet, Yueming Kelly Sun, Lilian Vincis Pereira Sanglard, Charles S. Bond, Ian Small
Summary: RNA editing in plants is carried out by PPR proteins, and a synthetic editing factor using PPR motifs has been successfully designed and demonstrated high specificity. This study paves the way for the design and application of programmable RNA editing factors based on plant PPR proteins.
COMMUNICATIONS BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Guo-Zhang Wu, Ralph Bock
Summary: Interactions between cellular compartments are critical for plant cell development and environmental adaptation. Plastids play a key role in energy production and biosynthesis of various metabolites, and signals originating from plastids regulate nuclear gene expression. Research on retrograde signaling has provided insight into cellular communication mechanisms within plant cells.
Article
Biochemistry & Molecular Biology
Xiang Wei, Yifang Zhu, Wenxiang Xie, Weiwei Ren, Yang Zhang, Hui Zhang, Shaojun Dai, Chao-Feng Huang
Summary: H2O2 plays a crucial regulatory role in aluminum resistance by affecting the stability of the key protein STOP1, which is involved in promoting aluminum resistance in plants.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)