Review
Biochemistry & Molecular Biology
Kaiyi Yang, Shenghui Feng, Zhijun Luo
Summary: Prostate cancer is a common and deadly cancer in males. Androgen-deprivation therapy is effective in early stage tumors, but many cases progress to metastatic castration-resistant prostate cancer. Existing clinical treatments for advanced prostate cancer have limitations, but gene therapy and viral therapy show promise in controlling the disease.
Review
Biotechnology & Applied Microbiology
Zongmin Zhao, Aaron C. Anselmo, Samir Mitragotri
Summary: Gene therapies using viral vectors have shown promising outcomes in treating various diseases that were once considered incurable. The use of viral vectors allows for efficient delivery of gene therapeutics into target cells, leading to the approval of multiple products and ongoing clinical trials exploring their therapeutic potential.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Klaus Mantwill, Florian Gerhard Klein, Dongbiao Wang, Sruthi Vasantamadhava Hindupur, Maximilian Ehrenfeld, Per Sonne Holm, Roman Nawroth
Summary: Oncolytic adenovirus therapy is an important novel treatment option for combating various cancers by exploiting the virus's replication and induction of cell death. Modifications in the adenoviral vectors contribute to restricting the virus's specificity and enhancing its therapeutic effects, leading to advancements in clinical trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Erwan Sallard, Lukas Schulte, Alexander van den Boom, Alexander Klimovitskii, Julius Knierer, Claudia Hagedorn, Maximilian Knocks, Wenli Zhang, Florian Kreppel, Anja Ehrhardt, Eric Ehrke-Schulz
Summary: Adenoviral vector Ad4 shows potential for gene therapy and cancer treatment. It demonstrates efficient transduction and long-lasting gene expression in cardiomyocytes and HPV-positive tumor organoids. By deleting transcription units, Ad4-based gene therapy vectors can be generated. This platform contributes to the development of improved adenoviral vectors based on non-canonical serotypes.
JOURNAL OF GENE MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Darya A. Savenkova, Aelita-Luiza A. Makarova, Igor K. Shalik, Dmitry V. Yudkin
Summary: Gene therapy is commonly used for treating incurable disorders, often using viral vectors to deliver genetic material. However, viral vectors have disadvantages such as immune response and disruption of cell metabolism, which require evaluation of side effects and safety. Many gene therapy techniques use non-coding RNA to regulate gene expression, but this can affect cellular miRNA pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Wataru Matsunaga, Akinobu Gotoh
Summary: Adenoviral vectors, although considered relatively obsolete, still possess advantages such as high cargo limit and transduction efficiency over newer viral vectors. This review discusses the use of modified fiber knob region and cancer-cell-specific promoters to enhance the affinity of adenoviral vectors for cancer cells and suppress expression of unwanted transgenes in non-malignant tissues.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Review
Virology
Elaine Y. L. Leung, Iain A. McNeish
Summary: Oncolytic viruses are emerging as a promising anti-cancer therapy that selectively replicate within malignant cells and induce immune responses. NK cells play a crucial role in oncolytic viral therapy, with their impact on OV efficacy being context-dependent. Advancements in understanding cancer and OV biology have led to the development of strategies to modulate NK activities for improving therapeutic benefits.
Review
Biochemistry & Molecular Biology
Shyambabu Chaurasiya, Yuman Fong, Susanne G. Warner
Summary: Oncolytic viruses are a new class of therapeutics that have shown promise in clinical trials, although their anti-tumor efficacy as monotherapy is modest. Most oncolytic viruses are well tolerated, even in immune-suppressed patients, at the currently defined highest feasible doses. Enhancing the potency of oncolytic viruses is necessary for more viruses to be used effectively in clinical settings, thus they are being studied in combination with other therapies for higher therapeutic benefits.
Review
Oncology
Marti Farrera-Sal, Laura Moya-Borrego, Miriam Bazan-Peregrino, Ramon Alemany
Summary: Cancer immunotherapy utilizing immune checkpoint inhibitors has shown effectiveness in various human cancers, but cold tumors often lack immune cells and are typically unresponsive. Oncolytic viruses, with their lytic and immunogenic mechanisms, have attracted interest as potential therapeutic approaches to heat or promote lymphocyte infiltration of cold tumors. This article reviews the use of oncolytic adenoviruses in cancer immunotherapy, focusing on immune responses triggered by these viruses against tumor antigens in preclinical and clinical settings, as well as considerations for clinical trial design and combination therapies with conventional treatments or other immunotherapies.
CLINICAL CANCER RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Jeong Heo, Ja-Der Liang, Chang Won Kim, Hyun Young Woo, I-Lun Shih, Tung-Hung Su, Zhong-Zhe Lin, So Young Yoo, Stanley Chang, Yasuo Urata, Pei-Jer Chen
Summary: OBP-301 is a modified oncolytic adenovirus that can replicate within cancer cells and cause their lysis. This phase I trial aimed to determine the safety and optimal dosage of OBP-301 in patients with advanced hepatocellular carcinoma. The study found that OBP-301 was well tolerated, with a maximum tolerated dose of 6 x 1012 viral particles. Injection of OBP-301 resulted in increased levels of CD8+ T cells and showed potential antitumor efficacy.
Article
Multidisciplinary Sciences
Alexander L. Ling, Isaac H. Solomon, Ana Montalvo Landivar, Hiroshi Nakashima, Jared K. Woods, Andres Santos, Nafisa Masud, Geoffrey Fell, Xiaokui Mo, Ayse S. Yilmaz, James Grant, Abigail Zhang, Joshua D. Bernstock, Erickson Torio, Hirotaka Ito, Junfeng Liu, Naoyuki Shono, Michal O. Nowicki, Daniel Triggs, Patrick Halloran, Raziye Piranlioglu, Himanshu Soni, Brittany Stopa, Wenya Linda Bi, Pierpaolo Peruzzi, Ethan Chen, Seth W. Malinowski, Michael C. Prabhu, Yu Zeng, Anne Carlisle, Scott J. Rodig, Patrick Y. Wen, Eudocia Quant Lee, Lakshmi Nayak, Ugonma Chukwueke, L. Nicolas Gonzalez Castro, Sydney D. Dumont, Tracy Batchelor, Kara Kittelberger, Ekaterina Tikhonova, Natalia Miheecheva, Dmitry Tabakov, Nara Shin, Alisa Gorbacheva, Artemy Shumskiy, Felix Frenkel, Estuardo Aguilar-Cordova, Laura K. Aguilar, David Krisky, James Wechuck, Andrea Manzanera, Chris Matheny, Paul P. Tak, Francesca Barone, Daniel Kovarsky, Itay Tirosh, Mario L. Suva, Kai W. Wucherpfennig, Keith Ligon, David A. Reardon, E. Antonio Chiocca
Summary: A phase I clinical trial in patients with rGBM showed that treatment with an oncolytic herpes virus (oHSV) enhanced anticancer immune responses, particularly in individuals with serology matching the virus.
Review
Immunology
Mingming Hu, Xuliang Liao, Yi Tao, Yaohui Chen
Summary: The treatment of recurrent glioma is challenging due to its molecular heterogeneity and resistance to commonly used treatments. Oncolytic viruses, which selectively replicate within tumor cells and stimulate the immune system, have emerged as a promising therapeutic option. Genetic modifications have been important in optimizing the efficacy of oncolytic herpes simplex virus and oncolytic adenovirus for recurrent gliomas. This review article summarizes these genetic modifications and aims to identify strategies to enhance the therapeutic benefits of oncolytic viruses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Selas T. F. Bots, Vera Kemp, Steve J. Cramer, Diana J. M. van den Wollenberg, Marten Hornsveld, Martine L. M. Lamfers, Gabri van der Pluijm, Rob C. Hoeben
Summary: The study aimed to develop oncolytic vectors with low prevalence of neutralizing immunity by isolating new nonhuman primate (nhp) Ads from great apes. Results showed that nhpAds of the HAdV-B species exhibited enhanced oncolytic potency compared to HAdV-C species and HAdV-C5 in vitro, making them potential alternatives for development of potent oncolytic Ad vectors with limited pre-existing neutralizing immunity in humans.
HUMAN GENE THERAPY
(2022)
Review
Oncology
Omeed Moaven, Christopher W. Mangieri, John A. Stauffer, Panos Z. Anastasiadis, Mitesh J. Borad
Summary: Studies on virus-based cancer treatment show limited success with monotherapy but genetic engineering offers potential in designing complementary therapies to enhance immune response. Recent research has highlighted the interactions between tumors and the immune system, pointing towards the potential of bioengineering virus platforms to improve immunotherapy effectiveness.
JCO PRECISION ONCOLOGY
(2021)
Article
Clinical Neurology
Victor M. Lu, Ashish H. Shah, Frederic A. Vallejo, Daniel G. Eichberg, Evan M. Luther, Sumedh S. Shah, Ricardo J. Komotar, Michael E. Ivan
Summary: A review of ClinicalTrials.gov database identified 29 oncolytic viral therapy trials for adult GBM before August 2020. Most trials were phase I, with a majority originating from the US and involving various malignant gliomas. Limited outcomes have been reported so far, but more data are expected as trials approach their recruitment completion time.
NEUROSURGICAL FOCUS
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)