Article
Biochemistry & Molecular Biology
Sihan Huang, Yanyun Zhao, Jun Liu
Summary: This study found that hypoxia induces the upregulation of HIF-1 alpha in the cochlea, leading to autophagy and protection against apoptosis in marginal cells. The HIF-1 alpha-BNIP3 pathway was identified as the mechanism responsible for these effects.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Isis Salviano Soares de Amorim, Juliana Alves Rodrigues, Pedro Nicolau, Sandra Konig, Carolina Panis, Adenilson de Souza da Fonseca, Andre Luiz Mencalha
Summary: This study investigated the molecular effects of DNA methyltransferase inhibitor in response to treatment in normoxia and hypoxia in breast cancer model. It was found that the inhibitor upregulated proinflammatory pathways, with a greater effect observed in normoxia than hypoxia. Specifically, NF-kappa B expression increased only in normoxia after treatment with the inhibitor, suggesting a potential context-dependent impact on inflammatory response in the tumor microenvironment.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Medicine, Research & Experimental
Fangfang Zhu, Jie Gao, Fuling Zeng, Yuling Lai, Xiaofeng Ruan, Gaopi Deng
Summary: This study investigated the protective effects and mechanisms of hyperoside against cyclophosphamide-induced ovarian damage and reduced fertility. The results showed that hyperoside can diminish follicular depletion, prevent excessive hypoxia and autophagy activation, and increase follicular reserve, thereby improving fertility in mice.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Cheng-Ning Ma, Lu-Lu Wo, Di-Fei Wang, Ci-Xiang Zhou, Jing-Chi Li, Xin Zhang, Xiu-Feng Gong, Chen-Long Wang, Ming He, Qian Zhao
Summary: This study screened and validated new hypoxia-related lncRNAs in hepatocarcinoma cells, identifying a hypoxia-activated lncRNA named HABON which plays a crucial role in promoting growth and proliferation of tumour cells in hypoxic conditions. Furthermore, HABON was found to be transcriptionally activated by HIF-1 alpha and could interact with HIF-1 alpha to affect transcription of its target genes, shedding light on the adaptive survival mechanism of tumour cells under hypoxia.
Article
Agronomy
Shuo Liu, Yu Bao, Hui Deng, Guanqing Liu, Yangshuo Han, Yuechao Wu, Tao Zhang, Chen Chen
Summary: DNA methylation is a crucial epigenetic modification that regulates gene expression and genome stability. The methylation inhibitor, AzaD, can effectively reduce genome-wide hypomethylation and cause a series of epigenetic variations.
Review
Pharmacology & Pharmacy
William B. Parker, Jaideep V. Thottassery
Summary: DNMT1 depletion is a rational goal in DNA methylation-dependent disorders, and current agents like decitabine and azacitidine have off-target activities limiting their use. Aza-T-dCyd is a promising candidate with less toxicity and higher therapeutic index for selectively depleting DNMT1. Further research is needed to understand its mechanism of action and potential clinical applications.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Karolina Luczkowska, Piotr Kulig, Klaudia Rusinska, Bartlomiej Baumert, Boguslaw Machalinski
Summary: This study investigates the effects of a methylation inhibitor on the proliferation of multiple myeloma cells and the development of resistance to treatment, finding that alterations in DNA methylation are associated with drug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Xiaoli Zhang, Gang Wang, Hui Li, Xiangming Jiang, Xiaoyong Zhao
Summary: The study demonstrated that 5-AZA-DC can alleviate the symptoms of preeclampsia by modulating the expression and interaction of STAT3, PTEN, and TSC2. It also promotes cell proliferation, inhibits apoptosis, and relieves the physiological abnormalities induced by PE.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Article
Genetics & Heredity
Yanlong Zhao, Xiaorong Zeng, Xinli Xu, Wenjing Wang, Lei Xu, Yiying Wu, Hang Li
Summary: The aim of this study was to investigate the effect of the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (Aza) on early renal injury. The results showed that low-dose Aza attenuated apoptosis and inflammation, and improved renal function. Furthermore, low-dose Aza upregulated the expression of Klotho protein, and the inhibition of Klotho protein reversed the protective effects of low-dose Aza on apoptosis and inflammation.
Article
Oncology
Huiying Cheng, Sijie Tang, Xueqi Lian, Hong Meng, Xiang Gu, Jiajia Jiang, Xiaohua Li
Summary: DNA methylation, mediated by DNA methyltransferase, affects gene expression and plays a role in malignant diseases. The classic inhibitor 5-Aza inhibits tumor cell proliferation and induces cell cycle arrest. It increases tumor suppressive proteins and transcription factors, while decreasing oncogene expression. These results suggest potential clinical applications in prostate cancer treatment.
Article
Immunology
Shramana M. Banerjee, Pilar Acedo, Soha El Sheikh, Rania Harati, Amelia Meecham, Norman R. Williams, Gareth Gerard, Mohammed R. S. Keshtgar, Alexander J. Macrobert, Rifat Hamoudi
Summary: Functional experiments showed that combining 5-ADC and PDT had a synergistic effect in vitro and increased cytotoxicity in TNBC 4T1 tumor cells. Treatment with 5-ADC/PDT showed histopathologically defined margins of necrosis and increased T cell expression, indicating an anti-tumor immune response. Bioinformatics analysis identified altered immune response biomarkers in TNBC compared to other breast cancer subtypes.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Environmental Sciences
Gerhard P. Aigner, Pamela Nenning, Birgit Fiechtner, Maja Srut, Martina Hoeckner
Summary: This study found that the demethylating agent Aza inhibits gene activity while Cd induces MT2 gene expression, but neither has an effect on global DNA methylation levels. Thus, Cd detoxification based on MT2 induction is not related to changes in DNA methylation.
Article
Oncology
Yang-Jie Zhang, Ran Xie, Jie Jiang, Li Zhai, Cong-Hui Yang, Jing Zhang, Xi Wang, Dong-Xue Chen, Hua-Tao Niu, Long Chen
Summary: The study demonstrates the methylation modification of GAS5 in melanoma and the reversible effect of 5-Aza-dC on this modification. Treatment with 5-Aza-dC inhibits cell proliferation, increases apoptosis, and reduces migration and invasion in melanoma cells. The antitumor effect of 5-Aza-dC in melanoma is related to the methylation modification of GAS5.
Article
Biochemistry & Molecular Biology
Alain Aguirre-Vazquez, Luis A. Salazar-Olivo, Xochitl Flores-Ponce, Ana L. Arriaga-Guerrero, Dariela Garza-Rodriguez, Maria E. Camacho-Moll, Ivan Velasco, Fabiola Castorena-Torres, Nidheesh Dadheech, Mario Bermudez de Leon
Summary: By utilizing epigenetic molecules and small molecules, the expression of pluripotency genes can be induced and methylation in the OCT4 promoter can be reduced in human somatic cells, enhancing reprogramming efficiency. Further studies are needed to elucidate the mechanisms by which these compounds exert their effects on reprogramming.
Article
Oncology
Kreon Koukoulas, Antonis Giakountis, Angeliki Karagiota, Martina Samiotaki, George Panayotou, George Simos, Ilias Mylonis
Summary: ERK-mediated phosphorylation of HIF-1 alpha enhances its interaction with NPM1, which influences the transcriptional activation of HIF-1 target genes. NPM1 and HIF-1 co-regulate genes enriched in different cancer types, and their expression correlates with hypoxic tumor status and patient prognosis.
MOLECULAR ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aleksandra Kaczorowska, Weronika Lamperska, Kaja Fraczkowska, Jan Masajada, Slawomir Drobczynski, Marta Sobas, Tomasz Wrobel, Kinga Chybicka, Radoslaw Tarkowski, Sebastian Kraszewski, Halina Podbielska, Wojciech Kalas, Marta Kopaczynska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Justyna Kutkowska, Leon Strzadala, Andrzej Rapak
Summary: Betulinic acid and betulin have shown promising activity against cancer cells, inhibiting viability and proliferation of non-small cell lung cancer (NSCLC) cells under normoxic and hypoxic conditions. The treatment induces G1 cell cycle arrest and increases apoptosis in NSCLC cells, with downregulation of the Wnt/beta-catenin pathway and deregulation of HIF-1 alpha and p53 expression levels observed. Additionally, betulinic acid reduces clonogenic activity under hypoxia, suggesting new strategies for treating hypoxic cancer tumors.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Yuriy Gerasymchuk, Wojciech Kalas, Jacek Arkowski, Lukasz Marciniak, Dariusz Hreniak, Edyta Wysokinska, Leon Strzadala, Marta Obremska, Larysa Tomachynski, Viktor Chernii, Wieslaw Strek
Summary: A new conjugate of gallato zirconium phthalocyanine complexes has been developed using alkilamino-modified SiO2 nanocarriers, showing potential for photodynamic therapy of atherosclerosis. The conjugate induces a cytotoxic effect on macrophages without corresponding to reactive oxygen species production, possibly due to SiO2 helping the photosensitizer enter cells effectively. This novel conjugate may be useful in the photodynamic therapy of coronary artery disease.
Article
Chemistry, Physical
Przemyslaw Sarelo, Maciej Duda, Marlena Gasior-Glogowska, Edyta Wysokinska, Wojciech Kalas, Halina Podbielska, Magdalena Wawrzynska, Marta Kopaczynska
Article
Biochemistry & Molecular Biology
Aleksandra Kaczorowska, Malgorzata Malinga-Drozd, Wojciech Kalas, Marta Kopaczynska, Stanislaw Wolowiec, Katarzyna Borowska
Summary: In this study, Polyamidoamine PAMAM dendrimer generation 3 (G3) was modified with biotin and glucoheptoamidated to obtain a series of conjugates with different numbers of biotin residues, which were used as host molecules to encapsulate 5-aminolevulinic acid. The interaction between host and guest molecules was characterized by NMR, revealing an increase in solubility of 5-aminolevulinic acid in water. Moreover, the encapsulates showed potential for delivering 5-aminolevulinic acid to cells without cytotoxic effects, and aqueous encapsulates were found to be more effective in this regard.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Malgorzata Grudzien, Aleksandra Pawlak, Justyna Kutkowska, Ewa Ziolo, Edyta Wysokinska, Wojciech Hildebrand, Bolena Obminska-Mrukowicz, Leon Strzadala, Andrzej Rapak
Summary: The CNK-89 cell line derived from a dog with NK cell neoplasia shows specific immunophenotyping and molecular characteristics. Treatment with IL-12 and other cytokines may impact the function and cytotoxic activity of CNK-89 cells.
VETERINARY AND COMPARATIVE ONCOLOGY
(2021)
Article
Chemistry, Medicinal
Marcin Maczynski, Andrzej Regiec, Aleksandra Sochacka-Cwikla, Iwona Kochanowska, Maja Kocieba, Ewa Zaczynska, Jolanta Artym, Wojciech Kalas, Michal Zimecki
Summary: The research demonstrated that RM33 is an effective immune suppressor with strong anti-inflammatory properties, showing no cell toxicity and stimulating splenocyte proliferation while exhibiting suppressive effects on specific cell types and enhancing the production of specific proteins.
Article
Pharmacology & Pharmacy
Michal Zimecki, Jolanta Artym, Wojciech Kalas, Leon Strzadala, Katarzyna Kaleta-Kuratewicz, Jan Kuryszko, Andrzej Kaszuba, Krzysztof Kaczmarek, Janusz Zabrocki
Article
Cell Biology
Jolanta Artym, Maja Kocieba, Ewa Zaczynska, Michal Zimecki, Wojciech Kalas, Leon Strzadala, Alicja Pawlak, Malgorzata Jelen, Beata Morak-Mlodawska, Krystian Pluta, Katarzyna Kaleta-Kuratewicz, Jan P. Madej, Piotr Kuropka, Jan Kuryszko
HISTOLOGY AND HISTOPATHOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)