Article
Multidisciplinary Sciences
Tomaz Zagar, Miha Pavsic, Aljaz Gaber
Summary: EpCANI is a cell-surface protein utilized as a carcinoma marker, involved in nuclear signaling via RIP. Research has shown that the oligomeric state of EpCAM is a determinant in RIP, with the disulfide-stabilized dimer being more resistant to cleavage than the monomeric form.
Article
Medicine, Research & Experimental
Klara Scupakova, Oluwatobi T. Adelaja, Benjamin Balluff, Vinay Ayyappan, Caitlin M. Tressler, Nicole M. Jenkinson, Britt S. R. Claes, Andrew P. Bowman, Ashley M. Cimino-Mathews, Marissa J. White, Pedram Argani, Ron M. A. Heeren, Kristine Glunde
Summary: The study reveals that N-glycosylation of breast cancer cells changes in a site-specific manner during metastasis, highlighting the clinical significance of high-mannose, fucosylated, and complex N-glycans as potential diagnostic markers and therapeutic targets in metastatic breast cancer.
Article
Cell Biology
Liu Yang, Qijun Wang, Qian Zhao, Fan Yang, Tingjiao Liu, Xiaohua Huang, Qiu Yan, Xuesong Yang
Summary: This study demonstrates that deglycosylated EpCAM promotes autophagy, inhibits proliferation, and induces apoptosis in breast cancer cells by activating the Akt/mTOR signaling pathway. These findings reveal a novel mechanism of deglycosylated EpCAM in breast cancer and suggest that a combination of autophagy modulation and EpCAM targeted therapy could be a promising therapeutic strategy.
Article
Chemistry, Multidisciplinary
Caleb A. H. Jones, Nathan D. Schley
Summary: The cleavage of alkyl ethers by hydrosilylation is demonstrated with stability of O-aryl glycosides under iridium- and borane-catalyzed conditions. A cationic bis(phosphine)iridium complex catalyzes the selective 3-demethylation of various 2,3,4-tri-O-methyl pyranoses.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Edgar Gonzalez-Rodriguez, Mia Zol-Hanlon, Ganka Bineva-Todd, Andrea Marchesi, Mark Skehel, Keira E. Mahoney, Chloe Roustan, Annabel Borg, Lucia Di Vagno, Svend Kjaer, Antoni G. Wrobel, Donald J. Benton, Philipp Nawrath, Sabine L. Flitsch, Dhira Joshi, Andres Manuel Gonzalez-Ramirez, Katalin A. Wilkinson, Robert J. Wilkinson, Emma C. Wall, Ramon Hurtado-Guerrero, Stacy A. Malaker, Benjamin Schumann
Summary: The polybasic cleavage motif for the protease furin is a major factor in human viral transmission of SARS-CoV-2 spike. Variants of concern (VOCs) have extensive mutations in the region preceding this motif. Besides furin, spikes from these variants rely on other proteases such as TMPRSS2 for maturation. Glycans near the cleavage site have implications for proteolytic processing and the effects of variant-borne mutations.
ACS CENTRAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Michelle N. Vu, Kumari G. Lokugamage, Jessica A. Plante, Dionna Scharton, Aaron O. Bailey, Stephanea Sotcheff, Daniele M. Swetnam, Bryan A. Johnson, Craig Schindewolf, R. Elias Alvarado, Patricia A. Crocquet-Valdes, Kari Debbink, Scott C. Weaver, David H. Walker, William K. Russell, Andrew L. Routh, Kenneth S. Plante, Vineet D. Menachery
Summary: The furin cleavage site (FCS) and the QTQTN motif are key factors for SARS-CoV-2 infection and pathogenesis. Deletion of the QTQTN motif reduces viral replication and pathogenicity by creating a shorter and more rigid peptide loop that is less accessible to host proteases. Disruption of the glycosylation in the QTQTN motif also attenuates virus replication. Therefore, the FCS, loop length, and glycosylation at the S1/S2 cleavage site are crucial for efficient SARS-CoV-2 replication and pathogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Inorganic & Nuclear
Lingzhi Qiu, Qiaoyu Zhang, Donghui Wei, Rongqiang Tian, Zheng Duan
Summary: Density functional theory calculations were used to investigate the fragmentation mechanism of 1-phenylselenyl-phosphirane complex. The results reveal that the reaction proceeds via an asynchronous concerted pathway, with the lone pair of the adjacent phosphorus atom playing a crucial role in the process.
DALTON TRANSACTIONS
(2022)
Article
Chemistry, Inorganic & Nuclear
Xue Chen, Hongfei Yin, Xiaoyong Yang, Weining Zhang, Dongdong Xiao, Zhen Lu, Yongzheng Zhang, Ping Zhang
Summary: Compared with the Haber Bosch process, the electrochemical nitrogen reduction reaction (NRR) offers a more environmentally friendly and sustainable route for ammonia synthesis. This study developed Co-doped Fe3S4 nanoflowers as a catalyst for NRR, achieving high Faradaic efficiency and NH3 yield rate. The introduction of Co atoms promoted N-2 absorption and accelerated the catalytic process, providing a pathway for enhancing ammonia synthesis using morphology and doping engineering for Fe-based electrocatalysts.
INORGANIC CHEMISTRY
(2022)
Article
Chemistry, Organic
Yuan Meng, Shunan Tao, Xiao-Yu Wu, Sha-Hua Huang, Ran Hong
Summary: Amino deoxysugars are widely found in nature and have significant biological functions, leading to numerous attempts to synthesize their unique structures. This study presents a novel approach to constructing amino deoxysugars embedded in natural products from a readily available lactic acid derivative, through a nitroso-ene-type cyclization to introduce a nitrogen atom into the carbon framework. This efficient synthesis provides an unprecedented synthetic route for exploring the nitroso-ene cyclization and accumulating intriguing amino deoxysugars.
Article
Multidisciplinary Sciences
Gowtham Thambra Rajan Premageetha, KanagaVijayan Dhanabalan, Sucharita Bose, Lavanyaa Manjunath, Deepthi Joseph, Aviv M. Paz, Samuel Grandfield, Vinod Nayak, Luis Bredeston, Jeff Abramson, Subramanian Ramaswamy
Summary: Nucleotide Sugar Transporters (NSTs) are important membrane transport proteins that play a crucial role in glycosylation by accumulating nucleotide sugars for polysaccharide biosynthesis. Dysfunction of NSTs affects glycosylation and can lead to developmental disorders, immune disorders, and increased susceptibility to infection. This study identified and cloned 18 members of the SLC35 family, and found that CtVrg4 from Chaetomium thermophilum functions as a GDP-mannose transporter with a high melting point temperature and affinity to bind to phosphatidylinositol lipids.
Article
Chemistry, Inorganic & Nuclear
Yufang Xie, Qiyi Miao, Wenhao Deng, Yilei Lu, Yinuo Yang, Xiaohui Chen, Rong-Zhen Liao, Shengfa Ye, Chen-Ho Tung, Wenguang Wang
Summary: A diamido-bridged dicobalt complex supported by a diamidonaphthalene ligand, Cp*Co2(2)(mu-1,8-C10H8(NH)(2)) (1), was synthesized and its reactivity towards redox transformations of the Co2N2 core was investigated. It was found that the Co(II)-Co(II) bond can be protonated, resulting in the formation of a bridging hydride [1H](+). The diamidonaphthalene ligand stabilizes the Co(II)Co(III) mixed-valent state (1(+)), which can bind CO to form [1-CO](+). Surprisingly, the mixed-valent complex also activates H2O to give a Co(III)Co(III) hydroxy complex [1-OH](+), accompanied by the release of H2.
INORGANIC CHEMISTRY
(2022)
Article
Immunology
Athanasios Papakyriakou, Anastasia Mpakali, Efstratios Stratikos
Summary: This study investigates the heterodimerization topology between endoplasmic reticulum aminopeptidases 1 and 2 (ERAP1 and ERAP2) using computational molecular dynamics calculations. The proposed model involves the exon 10 loop, suggesting a potential role and significance of ERAP1/ERAP2 molecular interactions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Min Pan, Vera Kohlbauer, Alexandra Blancke Soares, Henrik Schinke, Yuanchi Huang, Gisela Kranz, Tanja Quadt, Matthias Hachmeister, Olivier Gires
Summary: EpCAM is expressed in various tissues and cells, with interactions identified with regulators of endosomal trafficking and membrane recycling. Endocytosis and membrane recycling play a role in regulating EpCAM levels during differentiation and EMT induction in ESCs and carcinoma cells.
Article
Chemistry, Inorganic & Nuclear
Joseph A. Zurakowski, Brady J. H. Austen, Maeve C. Dufour, Moulika Bhattacharyya, Denis M. Spasyuk, Marcus W. Drover
Summary: This study reported the preparation of a novel cobalt (III) hydride and the influence of ligand design on reactivity, combining synchrotron-radiation crystallography and electrochemical studies for detailed investigation.
DALTON TRANSACTIONS
(2021)
Article
Immunology
Zhenlin Yang, He Tian, Fenglong Bie, Jiachen Xu, Zheng Zhou, Junhui Yang, Renda Li, Yue Peng, Guangyu Bai, Yanhua Tian, Ying Chen, Lei Liu, Tao Fan, Chu Xiao, Yujia Zheng, Bo Zheng, Jie Wang, Chunxiang Li, Shugeng Gao, Jie He
Summary: Our study demonstrates for the first time that higher expression of ERAP2 is tightly associated with the immuno-supportive microenvironment and can predict a favorable prognosis in SqCLC. Meanwhile, ERAP2 may be a promising immunotherapeutic target for patients with SqCLC.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Fei Tan, Xiao-hui Mo, Jian Zhao, Hui Liang, Zhong-jian Chen, Xiu-li Wang
JOURNAL OF NANOPARTICLE RESEARCH
(2017)
Article
Biotechnology & Applied Microbiology
Juan Castro, Luis Sanchez, Maria Teresa Nunez, Ming Lu, Tomas Castro, Hamid R. Sharifi, Christer Ericsson
Article
Cell Biology
Tong Liu, Jian Zhao, Cristian Ibarra, Maxime U. Garcia, Per Uhlen, Monica Nister
EUROPEAN JOURNAL OF CELL BIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Jian Zhao, Urban Lendahl, Monica Nister
CELLULAR AND MOLECULAR LIFE SCIENCES
(2013)
Article
Biotechnology & Applied Microbiology
Sam Ladjevardi, Gert Auer, Juan Castro, Christer Ericsson, Anders Zetterberg, Michael Haggman, Hans Wiksell, Hakan Jorulf
Article
Oncology
Tong Liu, Rong Yu, Shao-Bo Jin, Liwei Han, Urban Lendahl, Jian Zhao, Monica Nister
EXPERIMENTAL CELL RESEARCH
(2013)
Article
Multidisciplinary Sciences
Rong Yu, Tong Liu, Shao-Bo Jin, Chenfei Ning, Urban Lendahl, Monica Nister, Jian Zhao
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Rong Yu, Shao-Bo Jin, Urban Lendahl, Monica Nister, Jian Zhao
Article
Biochemistry & Molecular Biology
Rong Yu, Tong Liu, Chenfei Ning, Fei Tan, Shao-Bo Jin, Urban Lendahl, Jian Zhao, Monica Nister
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Cell Biology
Dan Wu, Sailan Wang, Daniel V. Oliveira, Francesca Del Gaudio, Michael Vanlandewijck, Thibaud Lebouvier, Christer Betsholtz, Jian Zhao, ShaoBo Jin, Urban Lendahl, Helena Karlstrom
Summary: Infantile myofibromatosis (IMF) is a benign tumor characterized by nonmetastatic tumors, with some cases associated with NOTCH3 gene mutations. Specifically, the NOTCH3L1519P mutation leads to enhanced downstream signaling but absence from the cell surface, instead accumulating in the endoplasmic reticulum. Treatment with chloroquine reduces secreted mutated extracellular domain and decreases signaling, while NOTCH3L1519P also upregulates PDGFRB expression in fibroblasts.
DISEASE MODELS & MECHANISMS
(2021)
Article
Biology
Rong Yu, Tong Liu, Shao-Bo Jin, Maria Ankarcrona, Urban Lendahl, Monica Nister, Jian Zhao
Summary: Our study reveals that MIEFs play a key role in regulating both fission and fusion machineries by interacting with fusion proteins Mfn1 and Mfn2, providing a novel mechanism for balancing mitochondrial dynamics in mammals.
Article
Medicine, General & Internal
Inti Peredo-Harvey, Jiri Bartek, Christer Ericsson, Koon-Chu Yaiw, Monica Nister, Afsar Rahbar, Cecilia Soderberg-Naucler
Summary: The presence of higher levels of HCMV-specific IgG antibodies in the plasma of brain tumor patients, except for those with secondary GBM, was observed. Furthermore, higher levels of HCMV-specific IgG were associated with prolonged survival time in patients with brain metastases.
MEDICINA-LITHUANIA
(2023)
Article
Oncology
Saad Misfer Al-Qahtani, Salah Eldin Gadalla, Min Guo, Christer Ericsson, Daniel Hagerstrand, Monica Nister
Summary: This study found that ANXA2 selectively co-immunoprecipitated with EpCAM in ER alpha(+) breast cancer cells MCF-7 and ZR-75-1. ANXA2 functions as a co-receptor for tissue plasminogen activator (tPA), and the association between EpCAM and ANXA2 affects the activity of tPA.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)