Article
Neurosciences
Jingmin Liu, Xinrui Ma, Xiaofang Hu, Jinkun Wen, Haowen Zhang, Jiawei Xu, Ye He, Xianghai Wang, Jiasong Guo
Summary: Our study found that RhoA knockdown or inhibition can alleviate the proliferation, migration, and differentiation of Schwann cells. In this study, we developed two lines of conditional RhoA knockout (cKO) mice to investigate the role of RhoA in Schwann cells during nerve injury and repair. Our results indicate that RhoA cKO in Schwann cells can accelerate axonal regrowth and remyelination after sciatic nerve injury, promoting the recovery of nerve conduction and hindlimb gait. Mechanistic studies revealed that RhoA cKO facilitates Schwann cell dedifferentiation via the JNK pathway, subsequently promoting Wallerian degeneration and stimulating the production of neurotrophins.
Article
Cell Biology
Ying Zou, Shu Wu, Fei Wen, Yuanlong Ge, Shengkang Luo
Summary: PGC-1a negatively regulates Schwann cell dedifferentiation and nerve degeneration after peripheral nerve injury, and its activation reduces peripheral nerve degeneration by inhibiting the expression of PON1.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Neurosciences
Ying Zou, Jiaqi Zhang, Jingmin Liu, Jiawei Xu, Lanya Fu, Xinrui Ma, Yizhou Xu, Shuyi Xu, Xianghai Wang, Jiasong Guo
Summary: SIRT6 acts as a negative regulator for Schwann cell dedifferentiation during Wallerian degeneration in injured peripheral nerve, with c-Jun identified as a direct downstream partner of SIRT6 in this process.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elisabetta Babetto, Bogdan Beirowski
Summary: In this article, recent research on pathological axon degeneration (pAxD) is reviewed, demonstrating that pAxD is regulated by an auto-destruction program linked to axonal bioenergetics. The article also highlights the emerging concept of intertwined axonal and glial energy metabolism. These findings are expected to encourage the development of new treatment strategies for neurodegeneration.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Biochemistry & Molecular Biology
Eun-Jung Sohn, Yun-Kyeong Nam, Hwan-Tae Park
Summary: The study revealed differential expression of miRNAs in cAMP-treated Schwann cells, with upregulation of miRNA363-5p targeting P2X purinoceptor 4 (P2RX4). The study suggests a double-negative feedback loop between miRNA363-5p and P2RX4 contributes to Schwann cell dedifferentiation and migration after nerve injury. Additionally, a P2RX4 antagonist was found to counteract myelin degradation after nerve injury, further increasing the levels of miRNA363-5p.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Marta Pellegatta, Paolo Canevazzi, Maria Grazia Forese, Paola Podini, Serena Valenzano, Ubaldo Del Carro, Angelo Quattrini, Carla Taveggia
Summary: ADAM17 in glial cells plays a crucial role in nerve regeneration by promoting remyelination through regulating p75(NTR). Its absence hampers effective nerve repair.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Neurosciences
Xiangling Li, Tieyuan Zhang, Chaochao Li, Wenjing Xu, Yanjun Guan, Xiaoya Li, Haofeng Cheng, Shengfeng Chen, Boyao Yang, Yuli Liu, Zhiqi Ren, Xiangyu Song, Zhibo Jia, Yu Wang, Jinshu Tang
Summary: Low-frequency electrical stimulation (ES) promotes nerve regeneration by accelerating Wallerian degeneration (WD) and upregulating the expression of neurotrophic factors, leading to accelerated early axonal and vascular regeneration while delaying muscle atrophy.
Article
Cell Biology
Clara Mutschler, Shaline V. Fazal, Nathalie Schumacher, Andrea Loreto, Michael P. Coleman, Peter Arthur-Farraj
Summary: Coculturing dissociated mouse Schwann cells (SCs) and dorsal root ganglion (DRG) neurons in microfluidic chambers can induce robust myelination and is a valuable technique for studying peripheral nervous system (PNS) myelination, injury, and regeneration. This model allows separate genetic manipulation of mouse SCs or neurons and can be used to complement in vivo mouse experiments. Live imaging of cocultures reveals that SCs are axo-protective and can clear axonal debris after traumatic axotomy.
JOURNAL OF CELL SCIENCE
(2023)
Article
Neurosciences
Deepak Prasad Gupta, Anup Bhusal, Md Habibur Rahman, Jae-Hong Kim, Youngshik Choe, Jaemyung Jang, Hyun Jin Jung, Un-Kyung Kim, Jin-Sung Park, Lee-so Maeng, Kyoungho Suk, Gyun Jee Song
Summary: This study reveals the essential role of EBP50 in the repair function of Schwann cells and regeneration following nerve injury. EBP50 deficiency leads to deficits in Schwann cell functions and delays nerve repair and functional recovery. Additionally, EBP50 enhances regeneration by binding and activating ErbB2.
PROGRESS IN NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yoon Kyung Shin, Young Rae Jo, Seoung Hoon Lee, Hwan Tae Park, Jung Eun Shin
Summary: After peripheral nerve injury, demyelinating Schwann cells release myelin debris and macrophages degrade myelin, leading to demyelination of degenerating axons, which is necessary for nerve regeneration. This study identifies ATP6V0D2, which is highly upregulated in injured mouse nerves, as a key player in this process. Knockout of ATP6V0D2 delays peripheral nerve demyelination and impairs myelin lipid digestion, while recruitment of macrophages and dedifferentiation of Schwann cells are unaffected. These findings highlight the specific role of ATP6V0D2 in macrophages during the demyelination process of Wallerian degeneration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Yangxue Yao, Yuting Wen, Yanjing Li, Jianwei Zhu, Taoran Tian, Qi Zhang, Dexuan Xiao, Yang Gao, Yunfeng Lin, Wei Wei, Xiaoxiao Cai
Summary: The study showed that tetrahedral framework nucleic acids (tFNAs) could regulate the neurorestorative pathway NGF/PI3 K/AKT, leading to activation of cell behaviors related to injured nerve repair. In vivo experiments demonstrated that tFNAs enhanced expression of axon and myelin marker proteins, improved histological recovery, and promoted efficient restoration of nerve conduction and muscle movement. tFNAs also exhibited excellent biocompatibility and endocytosis ability, suggesting promising potential for therapy of facial nerve injury and peripheral nerve injury.
Article
Neurosciences
Yi Wang, Nannan Gao, Yumei Feng, Min Cai, Yuting Li, Xi Xu, Huanhuan Zhang, Dengbing Yao
Summary: This study investigates the role of Prkcq in regulating nerve degeneration and regeneration, with findings showing that modulation of Prkcq expression can affect the function of Schwann cells and related signaling pathways. The results provide insights into the molecular mechanisms involved in Prkcq-mediated processes during peripheral nerve repair.
EXPERIMENTAL NEUROLOGY
(2021)
Review
Biochemistry & Molecular Biology
Anton Borger, Sarah Stadlmayr, Maximilian Haertinger, Lorenz Semmler, Paul Supper, Flavia Millesi, Christine Radtke
Summary: miRNAs play a crucial role in peripheral nerve injuries by regulating specific pathways and proteins, which is essential for understanding the molecular mechanisms of nerve regeneration and providing targets for precision medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Sulong Wang, Xilin Liu, Yueshu Wang
Summary: Peripheral nerve injury (PNI) is a common disease that leads to slow tissue regeneration and loss of nerve function. Platelet-rich plasma (PRP), a bioactive substance, can accelerate tissue regeneration by releasing growth factors. PRP has wide clinical applications and shows potential for peripheral nerve regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Neurosciences
Bo Jia, Wei Huang, Yu Wang, Peng Zhang, Zhiwei Wang, Ming Zheng, Tianbing Wang
Summary: The study revealed that Nogo protein promotes Schwann cell apoptosis and inhibits Schwann cell dedifferentiation following peripheral nerve injury, ultimately hindering peripheral nerve regeneration.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)