Article
Multidisciplinary Sciences
Ryuki Shimada, Hiroko Koike, Takamasa Hirano, Yuzuru Kato, Yumiko Saga
Summary: During murine germ cell development, NANOS2 plays a key role in suppressing the cell cycle and initiating sexually dimorphic differentiation. Using single-cell RNA sequencing, it was revealed that NANOS2 inhibits mTORC1 activity to suppress the cell cycle, potentially through target proteins like Ptma.
Article
Biology
Meysam Hassani Moghaddam, Neda Eskandari, Hossein Nikzad, Mohammad Miryounesi, Mohammad Karimian, Javad Amini Mahabadi, Mohammad Ali Atlasi
Summary: This study investigated the expression of genes associated with primordial germ cell development in differentiating mouse embryonic stem cells under the influence of progesterone and retinoic acid. It was found that RA treatment increased the expression of certain genes while decreasing others compared to the control group, and the combination of RA and progesterone (RA+P) further enhanced the differentiation process. Immunofluorescence studies showed higher expression levels in cells treated with RA+P, indicating a potential role for these factors in promoting germ cell differentiation.
JOURNAL OF BIOSCIENCES
(2021)
Article
Cell Biology
Michaela Mrugala Rothova, Alexander Valentin Nielsen, Martin Proks, Yan Fung Wong, Alba Redo Riveiro, Madeleine Linneberg-Agerholm, Eyal David, Ido Amit, Ala Trusina, Joshua Mark Brickman
Summary: Using single-cell transcriptomics, researchers have studied the different pathways of cell differentiation and identified an intermediate state in which cells transition from an extra-embryonic identity to an embryonic one. This finding has important implications for understanding organ differentiation in vitro.
NATURE CELL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Swati Mishra, Jasin Taelman, Mina Popovic, Laurentijn Tilleman, Evi Duthoo, Margot van der Jeught, Dieter Deforce, Filip van Nieuwerburgh, Bjoern Menten, Petra de Sutter, Annekatrien Boel, Susana M. Chuva De Sousa Lopes, Bjorn Heindryckx
Summary: This study found that continuous supplementation of ActA during differentiation of hESCs can promote differentiation into PGCLCs, and conversion to the 4i-state is beneficial for this process. The obtained PGCLCs may further differentiate into mature germ cells.
Review
Cell Biology
Geoffrey Brown
Summary: The activation of ATRA and RARs is crucial for proper development and decision-making of embryonic stem cells. RARa and RAR? have different roles in regulating hematopoiesis and stem cell maintenance, and their activation is influenced by the dose of ATRA and crosstalk with signaling events.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Zhuangzhuang Yang, Miao Yu, Xuechun Li, Yuanyuan Tu, Chunyan Wang, Wei Lei, Min Song, Yong Wang, Ying Huang, Fengyue Ding, Kaili Hao, Xinglong Han, Xuan Ni, Lina Qu, Zhenya Shen, Shijun Hu
Summary: This study demonstrates that retinoic acid inhibits angiogenesis and suppresses glycolysis in human embryonic stem cell-derived endothelial cells. The inhibitory effect is mediated by upregulation of fructose-1,6-bisphosphatase 1 (FBP1), a key enzyme in gluconeogenesis, and is dependent on the retinoic acid receptor (RAR). These findings suggest that FBP1 may serve as a novel therapeutic target for pathological angiogenesis.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Agriculture, Dairy & Animal Science
Ramon Cesar Botigelli, Carly Guiltinan, Rachel Braz Arcanjo, Anna Carolina Denicol
Summary: Pluripotent stem cells (PSC) can be obtained from embryos or adult somatic cells, and significant progress has been made in the livestock PSC field, including long-term culture and understanding cellular pluripotency. The germline is particularly important as it plays a crucial role in reproduction, and in vitro gametogenesis (IVG) could have significant implications for agriculture and reproduction. Many studies have been done on IVG in rodents, and this review discusses the current progress and challenges in livestock IVG.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Veterinary Sciences
Paloma Cordero, Alejandra Guerrero-Moncayo, Monica De los Reyes, Manuel Varas-Godoy, Jahaira Cortez, Cristian G. Torres, Victor H. Parraguez, Oscar A. Peralta
Summary: The in vitro derivation of gametes from stem cells has potential applications in animal reproduction for disseminating elite genetics, producing transgenic animals, and conserving endangered species. Mesenchymal stem cells (MSCs) are considered suitable for this purpose due to their differentiative capacity and potential for cell therapy. Retinoic acid (RA) and bone morphogenetic protein (BMP) 4 have been shown to upregulate specific markers associated with early germ cell differentiation in bovine fetal MSCs, suggesting their importance in gametogenesis. Furthermore, overexpression of DAZL, STRA8, and BOULE induced the upregulation of pluripotent markers and PIWIL2 in transfected bovine fetal AT-MSCs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Developmental Biology
Chun-Wei Feng, Guillaume Burnet, Cassy M. Spiller, Fiona Ka Man Cheung, Kallayanee Chawengsaksophak, Peter Koopman, Josephine Bowles
Summary: This study demonstrates the crucial role of retinoic acid responsive elements (RAREs) in initiating Stra8 expression in the fetal mouse ovary, along with the enhancement of Stra8 expression by the transcription factor DMRT1 in the presence of retinoic acid. The specific anterior-to-posterior pattern of Stra8 initiation in germ cells is faithfully recapitulated by the 2.9 kb Stra8 promoter, highlighting the importance of RAREs in regulating germ cell meiosis.
Article
Cell & Tissue Engineering
Kyung-Joo Seong, Seungho Choi, Hyun-Gwan Lee, Joon Haeng Rhee, Jin Ho Lee, Jeong-Tae Koh, Sun-Hun Kim, Won-Seok Choi, Ji-Yeon Jung, Won-Jae Kim
Summary: Toll-like receptor 5 plays a crucial role in regulating neurogenesis from mouse embryonic stem cells and adult hippocampal neural stem cells, promoting neural differentiation and inhibiting cell proliferation. TLR5 deficiency impairs fear memory performance in mice.
Article
Cell Biology
Chaoqing Huang, Yuan Wang
Summary: This study found that METTL14 plays an important role in osteoporosis by modulating the m6A modification of SMAD1. Knockdown of METTL14 suppressed osteogenesis and reduced bone mass. These findings provide evidence for METTL14 as a potential novel approach for improving osteoporosis.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Swati Mishra, Jasin Taelman, Yolanda W. Chang, Annekatrien Boel, Petra De Sutter, Bjorn Heindryckx, Susana M. Chuva De Sousa Lopes
Summary: The study found that a combination of TNAP and PDPN was effective in isolating populations of early FGCs and hPGCLCs in both sexes, with female hPGCLCs retaining stronger colony-forming ability in culture medium.
Article
Biochemistry & Molecular Biology
Magda Zachara, Pernille Y. Rainer, Horia Hashimi, Julie M. Russeil, Daniel Alpern, Radiana Ferrero, Maria Litovchenko, Bart Deplancke
Summary: This study characterizes the different subpopulations of adipose stem and precursor cells (ASPCs) and focuses on the CD142(+) ASPCs, which demonstrate non-adipogenic and anti-adipogenic properties. These characteristics are age-dependent and regulated by secretory factors and the retinoic acid signaling pathway.
Article
Cell Biology
Yanqiu Wang, Qin Na, Xihe Li, Wee-Wei Tee, Baojiang Wu, Siqin Bao
Summary: Retinoic acid induced a NELFA-mediated 2C-like state in mouse ESCs, characterized by 2C-specific transcriptional networks and the ability to contribute trophectoderm (TE) when injected into developing embryos. RA treatment also triggers DNA hypomethylation, active histone modification, suppressed glycolysis metabolism, and reduced protein synthesis activity of ESCs, indicating a broader role in 2C-like cells state.
CELL PROLIFERATION
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)