Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 427, Issue 1, Pages 171-177Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2012.09.036
Keywords
Cancer stem cell; Colorectal cancer; CD133; Two dimensional gel electrophoresis; Proteomics
Categories
Funding
- BK21 (Brain Korea 21)
Ask authors/readers for more resources
Recent reports have suggested that tumors are organized in heterogeneous populations. Within these populations, a small subpopulation of cells is more capable of initiating malignancy; these are called cancer stem cells. In this study, HT-29 cells were sorted according to the presence or absence of the cancer stem cell marker CD133. We confirmed that CD133+ cells possessed higher clonogenicity compared to CD133- cells. Furthermore, proteomic analysis identified 10 proteins, including actin-related protein 2/3 complex subunit 5-like and profilin 2. In conclusion, our data demonstrated that the expression of specific proteins associated with metastasis and invasion in CD133+ cells contributed to the sternness and tumorigenic properties of these cells. (C) 2012 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available