Article
Cell Biology
Joana Esteves de Lima, Reem Bou Akar, Myriam Mansour, Didier Rocancourt, Margaret Buckingham, Frederic Relaix
Summary: PAX3, a transcription factor in the paired-homeobox family, plays a crucial role in regulating muscle progenitor cells during embryonic development. Mutations in Pax3 can impair myotome formation, especially when combined with Pax3 null and Pax3 dominant-negative alleles. The adhesion molecule M-Cadherin is identified as a PAX3 target gene linked to myotome formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vivek Nilesh Shah, Julia Neumeier, Miguel Quevillon Huberdeau, Daniela M. Zeitler, Astrid Bruckmann, Gunter Meister, Martin J. Simard
Summary: The conserved serine/threonine kinases CK1A1 and CK2 regulate a phosphorylation cluster on the miRNA-specific AGO protein ALG-1, which is essential for miRISC-target RNA binding and silencing in Caenorhabditis elegans and humans.
Article
Cell Biology
Mira Merholz, Yongzhi Jian, Johannes Wimberg, Lea Gessler, Said Hashemolhosseini
Summary: Knockout of Csnk2a1 or Csnk2b results in diverse impairments of skeletal muscle biology, including muscle weakness, impaired neuromuscular transmission, enhanced regeneration, impaired oxidative metabolism, stimulated autophagic processes, and fragmented neuromuscular junctions.
Article
Biochemistry & Molecular Biology
Reena Ghildyal, Michael N. Teng, Kim C. Tran, John Mills, Marco G. Casarotto, Philip G. Bardin, David A. Jans
Summary: Respiratory syncytial virus (RSV) is a major cause of respiratory infections in infants and the elderly. The study identified protein kinase CK2 as a regulator of RSV matrix (M) protein's distribution between the nucleus and cytoplasm. Serine (S) 95 and threonine (T) 205 were found to be key CK2 sites. These findings provide insights for developing antiviral strategies targeting CK2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Zhuang Liu, Shuai Huang, Man Xu, Wenxue Zhang, Tuchen Guan, Qinghua Wang, Mei Liu, Jian Yao, Yan Liu
Summary: The study focused on the tail regeneration process in Gekko japonicus, finding that angiogenesis and axon regeneration occurred nearly simultaneously, followed by muscle tissue regeneration. PAX3 and PAX7 played key roles in myogenesis, while PCNA and PAX3 double-positive satellite cells were involved in tail regeneration.
JOURNAL OF MOLECULAR HISTOLOGY
(2021)
Review
Oncology
Scott W. Strum, Laszlo Gyenis, David W. Litchfield
Summary: CSNK2 is frequently dysregulated in cancer, influencing apoptosis evasion, proliferation, cell invasion/metastasis, and cell cycle control. It holds prognostic significance in various cancers, and inhibition in xenograft experiments shows positive treatment response.
BRITISH JOURNAL OF CANCER
(2022)
Review
Biochemistry & Molecular Biology
Andrea Baier, Ryszard Szyszka
Summary: Casein kinases are important factors in signaling pathways, inflammation, cancer, and neurological diseases, making them potential therapeutic targets. Recent studies have shown that the casein kinase 1 superfamily and CK2 play significant roles in the development of neurodegenerative pathologies. Overexpression of these kinases in the brain is closely related to neuronal and synaptic network functions. Therefore, the development of new substances for the treatment of these diseases is crucial.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Lucas M. Jozwick, Ashok P. Bidwai
Summary: The effects of Notch signaling in metazoan animals are mediated by the Enhancer of Split (E(spl)/HES) basic Helix-Loop-Helix-Orange (bHLH-O) repressors. This study explores the phosphorylation of these repressors and its impact on their function. It identifies E(spl)-M gamma as a new target of CK2 and reveals that the phosphorylation site is juxtaposed to the WRPW motif. The distinct positioning of CK2 and MAPK sites suggests that phosphorylation may contribute to the functional diversity of bHLH-O proteins.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Cell Biology
Peng Wang, Xupeng Liu, Ziyue Yao, Yu Chen, Lanfang Luo, Kun Liang, Jun-Hao Elwin Tan, Min-Wen Jason Chua, Yan-Jiang Benjamin Chua, Shilin Ma, Liping Zhang, Wenwu Ma, Shuqing Liu, Wenhua Cao, Luyao Guo, Lu Guang, Yuefan Wang, He Zhao, Na Ai, Yun Li, Chunwei Li, Ruiqi Rachel Wang, Bin Tean Teh, Lan Jiang, Kang Yu, Ng Shyh-Chang
Summary: During homeostasis and after injury, adult muscle stem cells (MuSCs) can activate and mediate muscle regeneration. Lin28a(+) MuSCs, which resemble embryonic muscle progenitors, have enhanced myogenic potency and self-renewal abilities compared to adult Pax7(+) MuSCs. These Lin28a(+) MuSCs can replenish the Pax7(+) MuSC pool and drive efficient muscle regeneration in adult mice.
Article
Biochemistry & Molecular Biology
John E. Chojnowski, Rongrong Li, Tiffany Tsang, Fatimah H. Alfaran, Alexej Dick, Simon Cocklin, Donita C. Brady, Todd I. Strochlic
Summary: Casein kinase 2 (CK2) is an evolutionarily conserved enzyme involved in cellular functions, and its dysregulation is linked to diseases like cancer. This study demonstrates that copper enhances CK2 activity by binding directly to the enzyme. Specific residues in the catalytic subunit are critical for copper-binding. Changes in cellular copper levels also affect CK2 activity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Chemistry, Medicinal
Christian Borgo, Luca Cesaro, Tsuyoshi Hirota, Keiko Kuwata, Claudio D'Amore, Thomas Ruppert, Renata Blatnik, Mauro Salvi, Lorenzo A. Pinna
Summary: CK2, a protein kinase with pleiotropic functions, is implicated in global human pathologies, particularly cancer. Selective inhibitors like CX4945 and GO289 show promise in controlling the CK2-dependent phosphoproteome.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Ondrej Mitrovsky, Denisa Myslivcova, Tereza Machackova-Lopotova, Adam Obr, Kamila Cermakova, Sarka Ransdorfova, Jana Brezinova, Hana Klamova, Marketa Zackova
Summary: Chronic myelogenous leukemia (CML) is a myeloproliferative disease characterized by the BCR-ABL oncogene. Despite the high performance of treatment with tyrosine kinase inhibitors (TKI), about 30% of patients develop resistance to the therapy. In this study, the researchers identified Casein Kinase 2 (CK2) as a potential target for CML therapy. They established new CML cell lines resistant to TKIs and found that CK2 activation was increased in these cells. Inhibition of CK2 using CX-4945 induced cell death in both parental and resistant CML cell lines, suggesting that CK2 represents a potential target for CML treatment.
Article
Biochemistry & Molecular Biology
Andrea Baier, Ryszard Szyszka, Monika Elzbieta Jach
Summary: Protein kinase CK2 is crucial for cell survival and the regulation of apoptosis. It is found that the yeast protein Asf1 inhibits CK2 activity through its poly-acidic sequence, which also influences cell growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Noa Barak-Broner, Dafna Singer-Lahat, Dodo Chikvashvili, Ilana Lotan
Summary: In this study, we found that the polybasic juxtamembrane region of syntaxin1A acts as a fusion clamp in PC12 cells and is required for depolarization-induced Ca2+-dependent opening of Syx. Phosphorylation of Syx at Ser-14 by casein kinase 2 was identified as a key molecular determinant for this process. The S14 phosphorylation confers the requirement for the close-to-open transition of Syx, which is crucial for both Ca2+-triggered release and spontaneous release.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Angel White, Anna McGlone, Rocio Gomez-Pastor
Summary: Huntington's Disease (HD) is a devastating neurodegenerative disorder with no current disease modifying therapies available. Recent research has focused on therapies to lower HTT expression, as well as the modulation of HTT post-translational modifications (PTMs). Phosphorylation of HTT has been proposed as a potential therapeutic option, with the protein kinase CK2 being described as one of the kinases involved. The role of CK2 in HD remains controversial, with pharmacological studies showing reduced phosphorylation and increased toxicity, while genetic approaches in mouse models have shown beneficial effects.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)