Article
Biochemistry & Molecular Biology
Lauren A. Griggs, Christopher A. Lemmon
Summary: Epithelial to Mesenchymal Transition (EMT) is a process in which epithelial cells transition to a motile and invasive mesenchymal phenotype. The EMT process is associated with an increase in fibronectin (FN) assembly and the localization of Transforming Growth Factor-beta 1 (TGF-beta 1) to FN fibrils. This study investigated the spatial gradients of EMT and FN fibrillogenesis in mammary epithelial cells cultured on square micropatterns in response to TGF-beta 1. The results showed that increasing TGF-beta 1 concentration induced a spatial gradient of both E-Cadherin and FN fibrils, and inhibition of FN fibril assembly globally reduced both E-Cadherin-containing adherens junctions and FN fibrillogenesis, but did not eliminate the spatial gradients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Katsuhiko Warita, Takuro Ishikawa, Akihiro Sugiura, Jiro Tashiro, Hiroaki Shimakura, Yoshinao Z. Hosaka, Ken-ichi Ohta, Tomoko Warita, Zoltan N. Oltvai
Summary: Statins, such as atorvastatin, can delay metastasis formation in vivo and attenuate the growth and proliferation of tumor cells, especially in those with a mesenchymal-like phenotype. In epithelial cancer cells treated with TGF-beta, atorvastatin-induced growth inhibition is stronger, and pre-treatment with atorvastatin can enhance this effect further by reducing HMGCR expression. Targeting HMGCR pharmacologically can strongly inhibit the growth of epithelial cancer cells treated with TGF-beta and potentially improve statin therapy-mediated attenuation of metastasis formation in vivo.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Yan Sun, Lang Xiong, Xiaoran Wang, Liping Wang, Baoxin Chen, Jingqi Huang, Mi Huang, Jieping Chen, Jing Wu, Shan Huang, Yizhi Liu
Summary: This study demonstrated that TGF-beta 2 induces autophagy flux during EMT progression of LECs, with autophagy activation enhancing fibrogenic responses and cell migration, while autophagy inhibition alleviates EMT markers and cell migration. Additionally, modulation of Smad signaling by autophagy was observed, and ATG7-silenced LECs showed antifibrosis effect induced by TGF-beta 2. Overall, intervention/inhibition of autophagy could attenuate TGF-beta 2-induced EMT in LECs, providing insights for preventing and treating fibrotic cataracts and other fibrotic diseases.
Article
Biochemistry & Molecular Biology
Li Han, Huaiqing Luo, Wenjie Huang, Jiang Zhang, Di Wu, Jinmei Wang, Jiao Pi, Chi Liu, Xiangping Qu, Huijun Liu, Xiaoqun Qin, Yang Xiang
Summary: Persistent injury and improper repair in bronchial epithelial cells contribute to airway inflammation and remodeling in asthma. E-cadherin plays a role in regulating the balance between EMT and MET during injury repair by inhibiting EMT features, enhancing MET features, and decreasing TGF-beta 1 secretion and Snail expression, ultimately facilitating epithelial repair.
Article
Biochemistry & Molecular Biology
Young Yun Jung, Chakrabhavi Dhananjaya Mohan, Huiyan Eng, Acharan S. Narula, Ojas A. Namjoshi, Bruce E. Blough, Kanchugarakoppal S. Rangappa, Gautam Sethi, Alan Prem Kumar, Kwang Seok Ahn
Summary: This study demonstrates that 2,3,5,6-Tetramethylpyrazine (TMP) can suppress the EMT program in colon cancer cells by regulating MnSOD and related proteins, and inhibit the proliferation, migration, and invasion of tumor cells.
Article
Biology
Ashish Kumar-Singh, Malgorzata Maria Parniewska, Nikolina Giotopoulou, Joman Javadi, Wenwen Sun, Tunde Szatmari, Katalin Dobra, Anders Hjerpe, Jonas Fuxe
Summary: Metastasis of tumor cells to vital organs is a major cause of cancer death, with inflammatory signals influencing an identity switch in tumor cells. Syndecan-1 plays a crucial role in regulating this switch, with nuclear localization of SDC1 associated with the regulation of EMT.
Article
Cell & Tissue Engineering
Le Phuong Hoang Anh, Ken Nishimura, Akihiro Kuno, Nguyen Thuy Linh, Tetsuo Kato, Manami Ohtaka, Mahito Nakanishi, Eiji Sugihara, Taka-Aki Sato, Yohei Hayashi, Aya Fukuda, Koji Hisatake
Summary: Somatic cell reprogramming proceeds through a series of events to generate induced pluripotent stem cells (iPSCs). In this study, the researchers found that the downregulation of Osr2 is crucial for efficient reprogramming by using a cell-based model. Osr2 acts as a novel EMT regulator and its downregulation diminishes TGF-beta signaling and activates Wnt signaling, promoting MET and acquiring pluripotency.
Article
Oncology
Hyun-Wook Lee, Cynthia C. Jose, Suresh Cuddapah
Summary: Nickel compounds, prevalent in the atmosphere due to industrial processes and fossil fuel burning, are associated with chronic inflammatory lung diseases, including asthma and lung cancer, primarily through the mechanism of epithelial-mesenchymal transition (EMT).
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Long Xu, Jian Wang, Buhan Liu, Jiaying Fu, Yuanxin Zhao, Sihang Yu, Luyan Shen, Xiaoyu Yan, Jing Su
Summary: Epithelial ovarian cancer has the highest mortality rate among gynecological malignant tumors, largely due to metastasis. This study found that HDAC9 plays different roles in different histological subtypes of ovarian cancer and may be a potential target for personalized treatment.
Article
Medicine, Research & Experimental
Ning Zhao, Minwei He, Wei Chen, Peng Jin, Lulu Cao, Jinhai Deng, Xu Cheng, Lu Wang
Summary: Our study identified FAM96A as a crucial regulator of tumor metastasis and epithelial-mesenchymal transition (EMT) by inhibiting the TGF-beta signaling pathway.
Article
Multidisciplinary Sciences
Shunhao Wang, Jingchao Li, Mei Chen, Liting Ren, Wenya Feng, Lining Xu, Xiaolan Chen, Tian Xia, Nanfeng Zheng, Sijin Liu
Summary: Palladium nanoplates inhibit lung metastasis by suppressing EMT signaling in breast cancer cells and interacting with TGF-beta proteins, leading to reduced downstream signaling activation. NIR irradiation enhances the inhibitory effects of PdPL on both primary tumors and metastasis.
NATIONAL SCIENCE REVIEW
(2021)
Article
Oncology
Xuke Qin, Lin Liu, Yanze Li, Hongbo Luo, Hui Chen, Xiaodong Weng
Summary: GOLM1 is upregulated in prostate cancer and promotes migration and invasion abilities by activating the TGF-beta 1/Smad2 signaling pathway to drive epithelial-mesenchymal transition (EMT). Therefore, GOLM1 has the potential to serve as a biomarker for prostate cancer diagnosis and prognosis prediction.
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
(2023)
Article
Pharmacology & Pharmacy
Maha-Hamadien Abdulla, Aminah Ahmad Alzailai, Mansoor-Ali Vaali-Mohammed, Rehan Ahmad, Sabiha Fatima, Ahmed Zubaidi, Thamer bin Traiki, Amer Mahmood, Reem Hamoud Alrashoudi, Zahid Khan
Summary: This study investigated the impact of a novel platinum complex on the invasion and migration of colorectal cancer cell lines. The results showed that the complex reduced cell motility and the expression of EMT markers involved in cell invasion and migration. This complex can potentially be used as an effective treatment for reducing metastasis in colorectal cancer.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Jingjing Qiu, Yanmei Wang, Wentao Guo, Ling Xu, Yakui Mou, Limei Cui, Fengchan Han, Yan Sun
Summary: The study investigated the role of TGF-beta 1-mediated epithelial-mesenchymal transition (EMT) in the pathogenesis of tympanosclerosis, finding that it plays an important role by activating the EMT pathway and causing a transformation of middle ear mucosal epithelial cells. Rats with tympanosclerosis models exhibited inflammation, fibrosis, and cell infiltration, along with significant changes in the expression levels of EMT-associated proteins.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Rohan Reddy Nagavally, Siddharth Sunilkumar, Mumtaz Akhtar, Louis D. Trombetta, Sue M. Ford
Summary: Chrysin demonstrates nephroprotective effects against CsA-induced renal dysfunction and fibrosis by inhibiting TGF-beta(1)-dependent EMT in proximal tubule cells through modulation of Smad-dependent and independent signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)