Review
Pharmacology & Pharmacy
Jianguo Feng, Jianlong Zhou, Yunxiao Lin, Wenhua Huang
Summary: This article highlights the importance of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) in regulating RNA metabolism and its dysregulation in various diseases. The selective binding sites of hnRNP A1 to RNA and DNA are summarized, along with the co-regulatory factors that interact with hnRNP A1. The potential therapeutic implications of targeting hnRNP A1 are discussed, including small-molecule drugs and biomedicines.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yun-Sang Tang, Wai-Kin So, Ka-Leung Andy Ng, Ka-Pun Chris Mok, Pang-Chui Shaw
Summary: This study identified host heterogeneous nuclear ribonucleoprotein C as an interacting partner of influenza A virus nucleoprotein and confirmed its negative regulatory role in viral growth. The findings not only enhance our understanding of the influenza virus transmission mechanism but also provide a new research direction for the development of anti-influenza drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Yixin Zou, Hanning Tang, Yi Miao, Huayuan Zhu, Li Wang, Lei Fan, Jianxin Fu, Wei Xu, Jianyong Li, Yi Xia
Summary: NOTCH1 mutation is a significant molecular abnormality in chronic lymphocytic leukemia (CLL), which leads to an unfavorable prognosis and poor response to chemoimmunotherapy. This study investigates the mechanism of adverse prognosis caused by NOTCH1 mutation from the perspective of heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1), a splicing factor. The results demonstrate that RNA splicing plays a vital role in NOTCH1-mutated CLL cells, and the overexpression of c-Myc-dependent hnRNPA1 promotes cell proliferation and inhibits apoptosis, contributing to the poor prognosis of patients with NOTCH1 mutation.
CHINESE MEDICAL JOURNAL
(2022)
Review
Cell Biology
Ulrich Kueck, Olga Schmitt
Summary: This article reviews RNA trans-splicing process in eukaryotes, focusing on trans-splicing of discontinuous group II introns in lower eukaryotes and plants' chloroplasts and mitochondria. It also discusses the origin of intronic sequences and the evolutionary relationship between chloroplast ribonucleoprotein complexes and the nuclear spliceosome.
Article
Immunology
Jiangwei Song, Dan Wang, Rong Quan, Jue Liu
Summary: This study investigated the relationship between Seneca Valley virus (SVV) infection and cellular hnRNP A1, demonstrating that SVV infection can induce the degradation and translocation of hnRNP A1, facilitating viral replication. This highlights the potential for controlling SVV infection by targeting hnRNP A1.
Article
Biochemistry & Molecular Biology
Jiaying Qiu, Ruobing Qu, Mengsi Lin, Jian Xu, Qingwen Zhu, Zhenyu Zhang, Junjie Sun
Summary: The study reveals a novel mechanism of hnRNP A1/2 in splicing regulation of SMN1/2 gene, where the binding position has an impact on splicing outcome.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2022)
Article
Chemistry, Multidisciplinary
Bin Zhang, Shao-han Xie, Jun-yi Hu, Si-jia Lei, Liang-hua Shen, Hong-tao Liu, Qing Zheng, Zhi-ming Zhang, Chun-lian Wu, Qiang Li, Feng Wang
Summary: This study found that SCRIB-S isoform was overexpressed in metastatic breast cancer cells and promoted cancer metastasis through the activation of the ERK pathway. hnRNP A1 promoted the skipping of SCRIB exon 16 by binding to the specific sequence on intron 15 of SCRIB. Transfection of an antisense oligodeoxynucleotide targeting this binding sequence effectively inhibited the production of SCRIB-S and suppressed breast cancer metastasis by reversing hnRNP A1-mediated ERK pathway activation.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Biochemistry & Molecular Biology
Tetsuyuki Takahashi, Yuri Ando, Hirona Ichikawa, Koichi Tsuneyama, Takao Hijikata
Summary: Our investigation revealed that undernutrition in cancer cells leads to a significant decrease in the protein level of hnRNP A1. This loss is reversible, specific to serum/glucose starvation, and occurs across different cell types and species. The stability of hnRNP A1 mRNA and CCND1 mRNA was not affected by this condition. The deletion of RRM1 in hnRNP A1 caused growth suppression, apoptosis induction, and autophagy, while the restoration of CCND1 rescued these effects.
Review
Biochemistry & Molecular Biology
Yang Hong-Guang, Tong Chun-Mei, Deng Hui-Min
Summary: hnRNP A1, as a crucial member of heterogeneous nuclear ribonucleoproteins, plays a significant role in regulating alternative splicing of genes related to cancer and neurological diseases. Additionally, it is involved in viral infection, cellular aging, and stress recovery, demonstrating its multifunctionality in various biological processes.
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Maxime Mazille, Katarzyna Buczak, Peter Scheiffele, Oriane Mauger
Summary: The nuclear envelope not only acts as a physical barrier between the nucleus and cytosol, but also plays a role in regulating gene expression. This study reveals that a majority of stable intron-retaining mRNAs are retained in the nucleus in mature mouse cortical neurons. Upon neuronal stimulation, some transcripts undergo degradation while others complete splicing and are exported to the cytosol, leading to rapid increases in protein levels. Different forms of stimulation mobilize distinct groups of intron-retaining transcripts, with selectivity arising from specific signaling pathways.
Article
Biochemistry & Molecular Biology
Maria Gabriela Pereira Dos Santos, Guilherme Henrique Gatti da Silva, Helder Yudi Nagasse, Cesar Seigi Fuziwara, Kimura Edna Teruko, Patricia Pereira Coltri
Summary: Heterogeneous nuclear ribonucleoproteins (hnRNPs) play important roles in gene expression regulation, particularly in pre-mRNA splicing. Overexpression of hnRNPs has been associated with various types of cancer. This study shows that overexpression of hnRNP A1 and hnRNP C in thyroid cancer cells directly affects the expression of miR-17-92 miRNAs, leading to increased cell proliferation, migration, and invasion.
Article
Neurosciences
Qingyang Zhang, Juan Zhang, Jin Ye, Xiaohui Li, Hongda Liu, Xiaolin Ma, Chao Wang, Keqiang He, Wei Zhang, Ji Yuan, Yingjun Zhao, Huaxi Xu, Qiang Liu
Summary: The study reveals that hnRNP L-DL is involved in regulating RNA splicing and its expression levels are decreased in aged mice and AD mice. Downregulation of L-DL impairs cognitive function, while overexpression improves cognition. This finding provides a new molecular mechanism for understanding and elucidating neurodegenerative diseases associated with AD.
MOLECULAR NEURODEGENERATION
(2021)
Article
Biochemistry & Molecular Biology
Courtney J. J. McCann, Nesrin M. M. Hasan, Teresita Padilla-Benavides, Shubhrajit Roy, Svetlana Lutsenko
Summary: Copper is an essential micronutrient that plays a critical role in mammalian growth and development. The study reveals that hnRNPA2/B1 acts as a negative regulator of ATP7A abundance by modulating its mRNA and protein levels, thus maintaining copper homeostasis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Medicine, General & Internal
Lianhua Liu, Wenyi Pang, Jixiang Liu, Shiqing Xu, Zhu Zhang, Risheng Hao, Jun Wan, Wanmu Xie, Xincao Tao, Peiran Yang, Lan Zhao, Zhenguo Zhai, Chen Wang
Summary: This study investigated the mechanisms of abnormal glucose metabolism in chronic thromboembolic pulmonary hypertension (CTEPH) and found that PKM2 and ROS play important roles in CTEPH. By suppressing the levels of PKM2 and ROS, the abnormal glucose metabolism, cell proliferation, and migration can be alleviated. The regulation of the hnRNPA1/PKM2 axis may be a potential therapeutic target for the treatment of CTEPH.
JOURNAL OF TRANSLATIONAL INTERNAL MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Irina Ritsch, Elisabeth Lehmann, Leonidas Emmanouilidis, Maxim Yulikov, Frederic Allain, Gunnar Jeschke
Summary: The interaction between hnRNP A1 and specific RNA induces liquid-droplet formation, which depends on RNA concentration and sequence. Additionally, point mutations in RNA affect local protein dynamics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Simon D. W. Frost, Brittany Rife Magalis, Sergei L. Kosakovsky Pond
MOLECULAR BIOLOGY AND EVOLUTION
(2018)
Article
Gastroenterology & Hepatology
Serena Y. C. Lin, Brittany Rife Magalis, Marco Salemi, Hsin-Fu Liu
JOURNAL OF VIRAL HEPATITIS
(2019)
Article
Immunology
Soo-Yon Rhee, Brittany R. Magalis, Leo Hurley, Michael J. Silverberg, Julia L. Marcus, Sally Slome, Sergei L. Kosakovsky Pond, Robert W. Shafer
OPEN FORUM INFECTIOUS DISEASES
(2019)
Article
Biochemistry & Molecular Biology
Sergei L. Kosakovsky Pond, Art F. Y. Poon, Ryan Velazquez, Steven Weaver, N. Lance Hepler, Ben Murrell, Stephen D. Shank, Brittany Rife Magalis, Dave Bouvier, Anton Nekrutenko, Sadie Wisotsky, Stephanie J. Spielman, Simon D. W. Frost, Spencer Muse
MOLECULAR BIOLOGY AND EVOLUTION
(2020)
Letter
Multidisciplinary Sciences
Carla Mavian, Sergei Kosakovsky Pond, Simone Marini, Brittany Rife Magalis, Anne-Mieke Vandamme, Simon Dellicour, Samuel V. Scarpino, Charlotte Houldcroft, Julian Villabona-Arenas, Taylor K. Paisie, Nidia S. Trovao, Christina Boucher, Yun Zhang, Richard H. Scheuermann, Olivier Gascuel, Tommy Tsan-Yuk Lam, Marc A. Suchard, Ana Abecasis, Eduan Wilkinson, Tulio de Oliveira, Ana I. Bento, Heiko A. Schmidt, Darren Martin, James Hadfield, Nuno Faria, Nathan D. Grubaugh, Richard A. Neher, Guy Baele, Philippe Lemey, Tanja Stadler, Jan Albert, Keith A. Crandall, Thomas Leitner, Alexandros Stamatakis, Mattia Prosperi, Marco Salemi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Sergei L. Kosakovsky Pond, Sadie R. Wisotsky, Ananias Escalante, Brittany Rife Magalis, Steven Weaver
Summary: Researchers have developed a simple method, Contrast-FEL, for identifying specific sites within genes evolving differently in a phylogenetic tree. Through extensive simulations, they have shown that this method has high power and can effectively control false positive rates when the model is correctly specified.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Virology
Shannan N. Rich, Veronica L. Richards, Carla N. Mavian, William M. Switzer, Brittany Rife Magalis, Karalee Poschman, Shana Geary, Steven E. Broadway, Spencer B. Bennett, Jason Blanton, Thomas Leitner, J. Lucas Boatwright, Nichole E. Stetten, Robert L. Cook, Emma C. Spencer, Marco Salemi, Mattia Prosperi
Article
Microbiology
Xingguang Li, Haizhou Liu, Brittany Rife Magalis, Sergei L. Kosakovsky Pond, Erik M. Volz
Summary: The unprecedented human norovirus GII.2 outbreak during the 2016-2017 winter season was analyzed using genetic sequencing from 7 countries dating back to 1976. The study revealed an increase in genetic diversity and at least eight distinct clusters, with fluctuating effective population size over the past 15 years. Viral evolutionary adaptation was not found to be responsible for the outbreak's scale and geographic distribution.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biology
Marta Giovanetti, Eleonora Cella, Francesca Benedetti, Brittany Rife Magalis, Vagner Fonseca, Silvia Fabris, Giovanni Campisi, Alessandra Ciccozzi, Silvia Angeletti, Alessandra Borsetti, Vittoradolfo Tambone, Caterina Sagnelli, Stefano Pascarella, Alberto Riva, Giancarlo Ceccarelli, Alessandro Marcello, Taj Azarian, Eduan Wilkinson, Tulio de Oliveira, Luiz Carlos Junior Alcantara, Roberto Cauda, Arnaldo Caruso, Natalie E. Dean, Cameron Browne, Jose Lourenco, Marco Salemi, Davide Zella, Massimo Ciccozzi
Summary: The study on SARS-CoV-2 transmission dynamics in Italy found that multiple lineages co-circulated, lockdown measures reduced infections but sporadic clusters continued to spread during summer.
COMMUNICATIONS BIOLOGY
(2021)
Article
Immunology
Massimiliano S. Tagliamonte, Carla Mavian, Kayvan Zainabadi, Melanie N. Cash, John A. Lednicky, Brittany Rife Magalis, Alberto Riva, Marie Marcelle Deschamps, Bernard Liautaud, Vanessa Rouzier, Daniel W. Fitzgerald, Jean William Pape, J. Glenn Morris, Marco Salemi
Summary: Haiti experienced an initial wave of COVID-19 in summer 2020, with an anti-SARS-CoV-2 IgG seropositivity rate of about 40%. The introduction of the P.1 variant in February 2021 led to exponential local dissemination within the unvaccinated population who had prior exposure to earlier SARS-CoV-2 lineages.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Biochemical Research Methods
Simone Marini, Carla Mavian, Alberto Riva, Mattia Prosperi, Marco Salemi, Brittany Rife Magalis
Summary: TARDiS is a novel phylogenetic tool that optimizes both genetic diversity and temporal distribution through a genetic algorithm.
Article
Immunology
Brittany Rife Magalis, Patrick Autissier, Kenneth C. Williams, Xinguang Chen, Cameron Browne, Marco Salemi
Summary: This study investigated the dynamic nature of the SIV population in the SIV/macaque model of AIDS, highlighting the adaptive immune response as the key factor explaining the behavior of the virus population. The relationship between the virus and immune cell populations revealed a predator-prey dynamic, with viral adaptation occurring in response to varying immune pressures over the course of infection progression.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Brittany R. Magalis, Carla Mavian, Massimiliano Tagliamonte, Shannan N. Rich, Melanie Cash, Alberto Riva, Julia C. Loeb, Michael Norris, David M. Amador, Yanping Zhang, Jerne Shapiro, Petr Starostik, Simone Marini, Paul Myers, David A. Ostrov, John A. Lednicky, J. Glenn Morris, Michael Lauzardo, Marco Salemi
Summary: The study investigates the effectiveness of vaccines in reducing symptoms and reveals breakthrough infections and the presence of new variants and low-frequency mutations. The findings suggest a relationship between the efficacy of vaccines and viral evolution.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Eleonora Cella, Sobur Ali, Sarah E. Schmedes, Brittany Rife Magalis, Simone Marini, Marco Salemi, Jason Blanton, Taj Azarian
Summary: This study explores the early emergence of the Delta variant of SARS-CoV-2 in Florida, USA through genomic analysis. Multiple independent introductions from different regions resulted in three distinct clades. The findings emphasize the importance of routine genomic surveillance and comprehensive epidemiological studies of emerging variants.
Article
Virology
Brittany Rife Magalis, Sergei L. Kosakovsky Pond, Michael F. Summers, Marco Salemi