Article
Cell Biology
Ruiyuan Xu, Jianlu Song, Rexiati Ruze, Yuan Chen, Xinpeng Yin, Chengcheng Wang, Yupei Zhao
Summary: This study reveals squalene epoxidase (SQLE) as a novel oncogene that promotes pancreatic cancer (PC) growth by mitigating endoplasmic reticulum stress and activating lipid raft-regulated Src/PI3K/Akt signaling pathway. SQLE facilitates cell proliferation, induces cell cycle progression, and inhibits apoptosis in vitro, while promoting tumor growth in vivo. SQLE inhibitors effectively suppress PC cell proliferation and xenograft tumor growth, highlighting the potential of SQLE as a therapeutic target for PC.
CELL DEATH & DISEASE
(2023)
Article
Plant Sciences
Yuling Liu, Luying Zhao, Xinyunxi He, Yi Shen, Na Wang, Sijing Hu, Jinlong Xu, Qiming Zhao, Quanlong Zhang, Luping Qin, Qiaoyan Zhang
Summary: Tiger bone, a traditional Chinese medicine, has been used to treat bone diseases. Artificial tiger bone JTG, approved for osteoporosis relief, has similar chemical profile and has been shown to protect bone loss. This study found that JTG proteins can promote osteogenesis and enhance autophagy through the PI3K/AKT and ER stress pathways.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Oncology
Xin Nie, Dawo Liu, Mingjun Zheng, Xiao Li, Ouxuan Liu, Qian Guo, Liancheng Zhu, Bei Lin
Summary: HERPUD1 is highly expressed in ovarian cancer and positively correlated with the expression of Lewis y antigen. HERPUD1 promotes ovarian cancer by promoting cell proliferation, inhibiting apoptosis, affecting the cell cycle, promoting autophagy, and inhibiting multiple signaling pathways.
Article
Oncology
Pan Xu, Aoran Luo, Chuan Xiong, Hong Ren, Liang Yan, Qiang Luo
Summary: This study verified the role of SCUBE3 in hepatocellular carcinoma (HCC) progression and demonstrated that SCUBE3 promotes HCC cell proliferation by regulating CCNE1. The study also identified alterations in signaling pathways associated with SCUBE3, including the TGF beta signaling pathway and the PI3K/AKT signaling pathway.
CANCER CELL INTERNATIONAL
(2022)
Article
Medicine, Research & Experimental
Xiaofeng Yu, Ying Li, Guodong Jiang, Jian Fang, Zhaolei You, Guangyuan Shao, Zheng Zhang, Aihong Jiao, Xiaonu Peng
Summary: This study revealed that FGF21 is upregulated in lung cancer and can promote cell growth and migration by activating the SIRT1/PI3K/AKT signaling pathway, indicating FGF21 as a potential therapeutic target for lung cancer treatment.
Article
Biochemistry & Molecular Biology
Jina Lee, Jinju Kim, Jae-Hyung Lee, Yong Min Choi, Hyeonil Choi, Hwan-Doo Cho, Guang-Ho Cha, Young-Ha Lee, Eun-Kyeong Jo, Byung-Hyun Park, Jae-Min Yuk
Summary: SIRT1 contributes to autophagy activation via AMPK and PI3K/AKT pathways, promoting anti-Toxoplasma responses. In Toxoplasma gondii infection, SIRT1 regulates the FoxO-autophagy axis and exhibits important anti-disease activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Tao Liu, Xiangsen Liang, Yu Sun, Shengzhuang Yang
Summary: The study revealed that SIRT1 expression was upregulated in EC, and rapamycin suppressed cell viability, migration, invasion, and the PI3K/AKT/mTOR signaling pathway in EC by negatively regulating SIRT1.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Cell Biology
Tomoyuki Fukuda, Tetsu Saigusa, Kentaro Furukawa, Keiichi Inoue, Shun-ichi Yamashita, Tomotake Kanki
Summary: The endoplasmic reticulum (ER) undergoes selective autophagy called reticulophagy or ER-phagy. Reticulon- and receptor expression enhancing protein (REEP)-like ER-shaping proteins, such as budding yeast Atg40, act as reticulophagy receptors to stabilize the phagophore on the ER by interacting with phagophore-conjugated Atg8. Hva22, a REEP family protein in fission yeast, promotes reticulophagy without Atg8-binding capacity and its role can be replaced by expressing Atg40 independently of its Atg8-binding ability.
Article
Multidisciplinary Sciences
Gunes Parlakgul, Ana Paula Arruda, Song Pang, Erika Cagampan, Nina Min, Ekin Guney, Grace Yankun Lee, Karen Inouye, Harald F. Hess, C. Shan Xu, Gokhan S. Hotamisligil
Summary: This study reveals the complex structural organization of organelles in liver tissue and highlights the significant alterations in obese mice. The functional importance of these structural changes on cellular and systemic metabolism is also demonstrated.
Article
Oncology
Wei Xu, Meiyi Lu, Siqi Xie, Dangui Zhou, Mei Zhu, Chaozhao Liang
Summary: Mounting evidence suggests that endoplasmic reticulum stress (ERS) plays a crucial role in modulating tumor-associated macrophages (TAMs) and shaping the immunosuppressive microenvironment. The communication between ER-stressed tumor cells and TAMs remains unclear, but exosomes have been identified as important mediators in intercellular communication. Therefore, this study aimed to investigate the impact of ER stress-related exosomes on prostate cancer cells in promoting macrophage infiltration and polarization. The findings demonstrated that TG-treated exosomes downregulated PD-L1 expression on macrophages and altered the expression of inflammatory cytokines and the PI3K/AKT pathway.
Article
Oncology
Jianbo Kuai, Na Zhang
Summary: In summary, Evo alleviates LPS-induced NPCs apoptosis and caspase-3 activation, reverses the ECM degradation, and reduces inflammation by upregulating SIRT1 and activating the PI3K/Akt pathway.
MOLECULAR MEDICINE REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Mingli He, Di Li, Caihong Fang, Qiong Xu
Summary: This study reveals that YTHDF1 is upregulated during inflammatory osteoclast differentiation and functions by inhibiting ER stress signaling pathways and Tnfrsf11a mRNA stability, therefore inhibiting inflammatory osteoclast differentiation and function.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Medicine, Research & Experimental
Yanqing Ye, Maosheng Liu, Fengfei Wu, Shiyu Ou, Weidong Wang, Jieying Fei, Fang Xie, Lan Bai
Summary: TRMT6 is overexpressed in HCC tissues and associated with TNM stage, T classification, and N classification. Overexpression of TRMT6 promotes HCC cell proliferation through the PI3K/AKT/mTOR axis in vivo and in vitro. Inhibition of TRMT6 reduces tumor growth and is related to the PI3K/AKT pathway.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Valerie Perea, Christian Cole, Justine Lebeau, Vivian Dolina, Kelsey R. Baron, Aparajita Madhavan, Jeffery W. Kelly, Danielle A. Grotjahn, R. Luke Wiseman
Summary: Endoplasmic reticulum (ER) stress and mitochondrial dysfunction are interconnected in the development of various diseases. The PERK signaling pathway, an arm of the unfolded protein response (UPR), plays a significant role in regulating mitochondrial biology during ER stress. This study reveals that PERK activity promotes adaptive remodeling of mitochondrial membrane phosphatidic acid (PA), leading to protective mitochondrial elongation in response to acute ER stress. These findings highlight a novel role for PERK in modulating mitochondrial phospholipids and demonstrate its involvement in adapting organellar shape during ER stress.
Article
Agriculture, Dairy & Animal Science
Xiaoya Li, Huali Chen, Zelin Zhang, Jiaxin Duan, Rongmao Hua, Xiaodi Li, Li Yang, Jianyong Cheng, Qingwang Li
Summary: This study investigated the therapeutic effect of isorhamnetin on ZEA-induced damage in porcine ovarian granulosa cells and elucidated its molecular mechanism. The results showed that isorhamnetin protected ovarian granulosa cells from ZEA-induced damage, promoting proliferation and alleviating apoptosis, endoplasmic reticulum stress, oxidative stress, and steroidogenesis disorder.
Article
Pharmacology & Pharmacy
Haruka Fujikawa, Taise Kawakami, Ryunosuke Nakashima, Aoi Nasu, Shunsuke Kamei, Hirofumi Nohara, Yuka Eto, Keiko Ueno-Shuto, Toru Takeo, Naomi Nakagata, Mary Ann Suico, Hirofumi Kai, Tsuyoshi Shuto
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2020)
Article
Multidisciplinary Sciences
Kentaro Oniki, Taisei Kawakami, Azusa Nakashima, Keishi Miyata, Takehisa Watanabe, Haruka Fujikawa, Ryunosuke Nakashima, Aoi Nasu, Yuka Eto, Noriki Takahashi, Hirofumi Nohara, Mary Ann Suico, Shunsuke Kotani, Yui Obata, Yuki Sakamoto, Yuri Seguchi, Junji Saruwatari, Tadashi Imafuku, Hiroshi Watanabe, Toru Maruyama, Hirofumi Kai, Tsuyoshi Shuto
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Kentaro Oniki, Hirofumi Nohara, Ryunosuke Nakashima, Yui Obata, Narumi Muto, Yuki Sakamoto, Keiko Ueno-Shuto, Tadashi Imafuku, Yu Ishima, Hiroshi Watanabe, Toru Maruyama, Koji Otake, Yasuhiro Ogata, Mary Ann Suico, Hirofumi Kai, Tsuyoshi Shuto, Junji Saruwatari
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Haruka Fujikawa, Yuki Sakamoto, Natsuki Masuda, Kentaro Oniki, Shunsuke Kamei, Hirofumi Nohara, Ryunosuke Nakashima, Kasumi Maruta, Taisei Kawakami, Yuka Eto, Noriki Takahashi, Toru Takeo, Naomi Nakagata, Hiroshi Watanabe, Koji Otake, Yasuhiro Ogata, Naoko H. Tomioka, Makoto Hosoyamada, Tappei Takada, Keiko Ueno-Shuto, Mary Ann Suico, Hirofumi Kai, Junji Saruwatari, Tsuyoshi Shuto
Article
Multidisciplinary Sciences
Mariam Piruzyan, Ihori Shitanda, Yuichiro Shimauchi, Go Okita, Yu Tsurekawa, Masataka Moriuchi, Yoshio Nakano, Keisuke Teramoto, Mary Ann Suico, Tsuyoshi Shuto, Hirofumi Kai
Article
Urology & Nephrology
Misato Kamura, Tomohiko Yamamura, Kohei Omachi, Mary Ann Suico, Kandai Nozu, Shota Kaseda, Jun Kuwazuru, Tsuyoshi Shuto, Kazumoto Iijima, Hirofumi Kai
KIDNEY INTERNATIONAL REPORTS
(2020)
Article
Biology
Hiromitsu Iwata, Tsuyoshi Shuto, Shunsuke Kamei, Kohei Omachi, Masataka Moriuchi, Chihiro Omachi, Toshiyuki Toshito, Shingo Hashimoto, Koichiro Nakajima, Chikao Sugie, Hiroyuki Ogino, Hirofumi Kai, Yuta Shibamoto
JOURNAL OF RADIATION RESEARCH
(2020)
Article
Pharmacology & Pharmacy
Yoshihiro Kobashigawa, Mana Namikawa, Mitsuhiro Sekiguchi, Yuki Inada, Soichiro Yamauchi, Yuu Kimoto, Kyo Okazaki, Yuya Toyota, Takashi Sato, Hiroshi Morioka
Summary: The constitutive active/androstane receptor (CAR) is a nuclear receptor involved in drug metabolism regulation. A novel system was developed for rapid evaluation of drug interactions with CAR, using differential scanning fluorometry (DSF) to confirm direct interactions. This method is easy to set up and beneficial for drug discovery purposes.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Pharmacology & Pharmacy
Yoshio Nakano, Masataka Moriuchi, Yutaro Fukushima, Kyotaro Hayashi, Mary Ann Suico, Hirofumi Kai, Go Koutaki, Tsuyoshi Shuto
Summary: Caenorhabditis elegans is widely used for longevity studies, and recent advances have allowed for automated monitoring of its behavior. This study utilized a live-cell analysis system to determine parameters of nematode lifespans and identified distinct longevity patterns and clusters in wild-type C. elegans. The research also highlighted the important role of AMP-activated protein kinase in regulating healthspan in C. elegans.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Yusei Yamada, Yoichi Ishitsuka, Yuki Kondo, Shuichi Nakahara, Asami Nishiyama, Toru Takeo, Naomi Nakagata, Keiichi Motoyama, Taishi Higashi, Hidetoshi Arima, Shunsuke Kamei, Tsuyoshi Shuto, Hirofumi Kai, Yuji Hayashino, Masatake Sugita, Takeshi Kikuchi, Fumio Hirata, Toru Miwa, Hiroki Takeda, Yorihisa Orita, Takahiro Seki, Tomoko Ohta, Yuki Kurauchi, Hiroshi Katsuki, Muneaki Matsuo, Katsumi Higaki, Kousaku Ohno, Shirou Matsumoto, Takumi Era, Tetsumi Irie
Summary: The study showed that HP-gamma-CD forms a stable 1:1 inclusion complex with cholesterol, and has almost equivalent therapeutic effects in treating NPC compared to HP-beta-CD. HP-gamma-CD has lower toxicity than HP-beta-CD, and demonstrates the same efficacy in treating NPC models.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Kohei Omachi, Shota Kaseda, Tsubasa Yokota, Misato Kamura, Keisuke Teramoto, Jun Kuwazuru, Haruka Kojima, Hirofumi Nohara, Kosuke Koyama, Sumio Ohtsuki, Shogo Misumi, Toru Takeo, Naomi Nakagata, Jian-Dong Li, Tsuyoshi Shuto, Mary Ann Suico, Jeffrey H. Miner, Hirofumi Kai
Summary: Metformin is commonly used for treating type 2 diabetes, and recent studies have suggested its potential in ameliorating tumor progression, inflammatory diseases, and fibrosis. This study found that metformin not only improved non-diabetic glomerular diseases and chronic kidney disease phenotypes, but also influenced metabolic and inflammatory pathways in the transcriptome analysis.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yoshihiro Kobashigawa, Toshiya Ohara, Kosuke Morita, Yuya Toyota, Teruya Nakamura, Shunsuke Kotani, Takao Arimori, Soichiro Yamauchi, Chenjiang Liu, Masaya Kitazaki, Yukari Wakeyama-Miyazaki, Yoshiaki Suwa, Makiyo Uchida-Kamekura, Natsuki Fukuda, Takashi Sato, Makoto Nakajima, Junichi Takagi, Yuriko Yamagata, Hiroshi Morioka
Summary: The research team developed a single-chain Fv (scFv) antibody against cytotoxic GA-pyridine and examined its specificity and antigen recognition using various techniques. The study demonstrated that GA-pyridine specifically bound to 73MuL9-scFv and the binding was an exothermic and enthalpy driven reaction, involving multiple specific interactions. Crystallographic analysis revealed CH-pi and hydrogen bond interactions between the Fv fragment of 73MuL9-scFv and GA-pyridine. Further studies are needed to clarify the relevance of GA-pyridine to diabetic complications.
JOURNAL OF BIOCHEMISTRY
(2021)
Article
Urology & Nephrology
Jun Kuwazuru, Mary Ann Suico, Kohei Omachi, Haruka Kojima, Misato Kamura, Shota Kaseda, Teppei Kawahara, Yuki Hitora, Hikaru Kato, Sachiko Tsukamoto, Mikiyo Wada, Toshifumi Asano, Shunsuke Kotani, Makoto Nakajima, Shogo Misumi, Yuya Sannomiya, Jun Horizono, Yuimi Koyama, Aimi Owaki, Tsuyoshi Shuto, Hirofumi Kai
Summary: This study provides a novel therapeutic candidate, alisporivir, for Alport syndrome (AS) by enhancing the secretion of mutant 345(IV) and reveals the importance of cyclophilin D in this process. It suggests that alisporivir could be a safer option than cyclosporin A for AS treatment.
Article
Urology & Nephrology
Shota Kaseda, Yuya Sannomiya, Jun Horizono, Jun Kuwazuru, Mary Ann Suico, Sayaka Ogi, Ryoko Sasaki, Hidetoshi Sunamoto, Hirohiko Fukiya, Hayato Nishiyama, Misato Kamura, Saki Niinou, Yuimi Koyama, Futoshi Nara, Tsuyoshi Shuto, Kazuhiro Onuma, Hirofumi Kai
Summary: UBE-1099 significantly ameliorates the progressive phenotype in Alport mice. Our results revealed the efficacy of Keap1-Nrf2 PPI inhibitor for glomerulosclerosis and present a potential therapeutic drug for CKD.
Article
Urology & Nephrology
Yuya Sannomiya, Shota Kaseda, Misato Kamura, Hiroshi Yamamoto, Hiroyuki Yamada, Masataka Inamoto, Jun Kuwazuru, Saki Niino, Tsuyoshi Shuto, Mary Ann Suico, Hirofumi Kai
Summary: This study investigated the involvement of DDR2 in Alport syndrome, finding that reducing Ddr2 expression with DDR2 ASO did not significantly improve proteinuria or BUN levels, nor did it ameliorate renal injury, inflammation and fibrosis in AS mice. This suggests that DDR2 may not play a critical role in the progression of Alport syndrome.