Review
Oncology
Sintayehu Tsegaye Tseha
Summary: Adenoviral vectors have great potential in cancer therapy by changing the tumor microenvironment and directly killing tumor cells. Some adenoviral vectors have been approved and shown to be effective in clinical practice, while others that enhance immune response through expressing immune-modulatory molecules and antigens also show promising outcomes. However, the use of adenoviral vectors in gene therapy is limited by certain factors, and innovative strategies need to be continuously developed.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Moonkyung Kang, Ayoung Song, Jiyoung Kim, Se Hun Kang, Sang-Jin Lee, Yeon-Soo Kim
Summary: In this study, a gibbon ape leukemia virus pseudotyped semi-replication-competent retrovirus vector system (spRRV) was established, which showed considerable improvement in gene transfer and spreading efficiencies. It has the potential for clinical trials in the treatment of malignant solid tumors.
Article
Oncology
Theophilos Tzaridis, Niklas Schaefer, Johannes Weller, Joachim-Peter Steinbach, Uwe Schlegel, Sabine Seidel, Michael Sabel, Peter Hau, Clemens Seidel, Dietmar Krex, Roland Goldbrunner, Joerg-Christian Tonn, Oliver Grauer, Sied Kebir, Matthias Schneider, Christina Schaub, Hartmut Vatter, Christoph Coch, Martin Glas, Rolf Fimmers, Torsten Pietsch, Guido Reifenberger, Ulrich Herrlinger, Joerg Felsberg
Summary: The CeTeG/NOA-09 trial demonstrated a survival benefit for glioblastoma patients with MGMT promoter methylation ratios >2 who received combined CCNU/TMZ therapy. The prognostic value of qMSP ratio, as well as the concordance of MGMT methylation results obtained by different methods, were evaluated. Different qMSP ratio cutoffs may impact treatment efficacy, with lower ratios potentially showing larger benefits from CCNU/TMZ therapy. Acceptable concordance rates were observed among different MGMT methylation assays, allowing for potential consideration of alternative methods for therapeutic decision-making.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Genetics & Heredity
Stefaan W. Van Gool, Jennifer Makalowski, Michael Bitar, Peter Van de Vliet, Volker Schirrmacher, Wilfried Stuecker
Summary: This study analyzed the prognosis of IDH1 wild-type MGMT promoter-unmethylated GBM patients and found that individualized multimodal immunotherapy may help improve the overall survival of patients. Retrospective analysis was performed on 32 patients who met the criteria, and the results showed that the extent of resection had a certain impact on the survival of patients.
GENES AND IMMUNITY
(2022)
Article
Neurosciences
Li-Ya Jiang, Guan-Hao Wang, Jing-Jiao Xu, Xiao-Li Li, Xiao-Yan Lin, Xiang Fang, Hong-Xu Zhang, Mei Feng, Chun-Ming Jiang
Summary: This study reveals the importance of LINC00473 in regulating temozolomide (TMZ) resistance in glioblastoma (GB) and its potential mechanism. By regulating the expression of CEBP alpha and MGMT, LINC00473 promotes the formation of chemoresistance. Furthermore, LINC00473 can transfer chemoresistance to adjacent sensitive cells through exosomes.
Article
Genetics & Heredity
Peng Ren, Jing-Ya Wang, Zhi-Rui Zeng, Nan-Xi Li, Hong-Lei Chen, Xin-Ge Peng, Ujjal K. Bhawal, Wen-Zhi Guo
Summary: In this study, a set of hypoxia-driven genes were identified and a novel gene signature was constructed using RNA-seq data, which showed remarkable prognostic value for glioma patients. These hypoxia-driven genes were also found to be associated with TMZ resistance.
FRONTIERS IN GENETICS
(2022)
Article
Chemistry, Multidisciplinary
Minkyung Kim, Jungju Oh, Youngki Lee, Eun-Hye Lee, Seung Hwan Ko, Ji Hoon Jeong, Chang Hwan Park, Minhyung Lee
Summary: In this study, self-replicating mRNA (Rep-mRNA) was developed using a replicon system to prolong gene expression for the treatment of ischemic stroke. The Rep-mRNA/DA-PEI complex showed higher and longer expression of HO1 compared to other complexes, leading to decreased apoptosis and infarct size in an animal model of ischemic stroke.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Clinical Neurology
Waqar Haque, Elaine Thong, Sara Andrabi, Vivek Verma, E. Brian Butler, Bin S. Teh
Summary: This study is the largest of its kind to examine the prognostic and predictive impact of MGMT methylation (mMGMT) amongst patients with Grade 3 Glioma. The results suggest that mMGMT is prognostic and possibly a predictive biomarker. However, only 5% of patients nationwide with Grade 3 Glioma are tested for MGMT.
JOURNAL OF CLINICAL NEUROSCIENCE
(2021)
Article
Oncology
Hiroki Kushiya, Kei Hiraoka, Tomohiro Suzuki, Kazuho Inoko, Akihito Inagaki, Hiroki Niwa, Katsunori Sasaki, Toru Nakamura, Takahiro Tsuchikawa, Toshiaki Shichinohe, Douglas J. J. Jolly, Noriyuki Kasahara, Satoshi Hirano
Summary: This study applies a gene therapy strategy based on retroviral replicating vectors to lung cancer treatment and demonstrates significant therapeutic benefit both in vitro and in vivo. The retroviral vectors efficiently deliver genes to lung cancer cells and effectively kill the cancer cells upon exposure to a prodrug. This novel strategy has potential to address the unmet medical need for more effective ways to treat lung cancer.
Article
Biochemistry & Molecular Biology
Hiroaki Fujino, Emiko Sonoda-Fukuda, Lisa Isoda, Ayane Kawabe, Toru Takarada, Noriyuki Kasahara, Shuji Kubo
Summary: Retroviral replicating vectors (RRVs) have the ability to selectively replicate and introduce prodrug-activating genes into tumor cells, making them potentially beneficial for treating gastric cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nikola Jovanovic, Milica Lazarevic, Vladimir J. Cvetkovic, Vesna Nikolov, Jelena Kostic Peric, Milena Ugrin, Sonja Pavlovic, Tatjana Mitrovic
Summary: A single-institution observational study evaluated the prognostic relevance of the methylation status of MGMT in diffuse gliomas. The study found that younger patients with surgically resected gliomas and temozolomide (TMZ) adjuvant chemotherapy had a better prognosis. The chosen method and cut-off value determination for methylation status affected the results. The study recognized MGMT promoter methylation as a positive prognostic factor, but further verification in a larger population is needed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Sena Ikemoto, Fuminori Sakurai, Sora Tokuoka, Tomoki Yamashita, Kosuke Takayama, Kazuaki Hoshi, Takahiro Okabe, Issei Sumiyoshi, Shinsaku Togo, Kazuhisa Takahashi, Masashi Tachibana, Hiroyuki Mizuguchi
Summary: Circulating tumor cells (CTCs) are present in the blood of cancer patients and are closely correlated with patient prognosis and treatment responses. Therefore, CTCs have been recognized as a novel biomarker for early detection of cancer and monitoring of treatment responses. However, it is crucial to develop an accurate and highly sensitive CTC detection method due to the low abundance of CTCs in patient blood samples. The researchers in this study developed four types of novel GFP-expressing conditionally replicating adenoviruses (rAds) and successfully visualized CTCs in the blood samples of lung cancer patients.
Article
Neurosciences
Chixing Luo, Chuansheng Nie, Yibin Zeng, Kang Qian, Xudong Li, Xuan Wang
Summary: In this study, it was found that LINC01564 upregulates NFE2L2 expression to inhibit ferroptosis, thereby promoting temozolomide resistance in glioma cells.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Medicine, General & Internal
Sixuan Chen, Yue Xu, Meiping Ye, Yang Li, Yu Sun, Jiawei Liang, Jiaming Lu, Zhengge Wang, Zhengyang Zhu, Xin Zhang, Bing Zhang
Summary: This study aimed to predict MGMT promoter methylation in diffuse gliomas using a deep learning approach with MRI radiomics. The T1CE combined with ADC model based on the ROI of the tumor core exhibited the best performance among all models, with the highest average accuracy (0.91) and AUC (0.90).
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Oncology
Connor J. Kinslow, Markus D. Siegelin, Fabio M. Iwamoto, Matthew Gallitto, Alfred I. Neugut, James B. Yu, Simon K. Cheng, Tony J. C. Wang
Summary: This study found that the unmethylated status of the MGMT promoter is associated with poorer survival in patients with IDH-wildtype gliomas, but does not significantly affect the survival of patients with IDH-mutant gliomas.
JOURNAL OF NEURO-ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)