Article
Endocrinology & Metabolism
Akane Inoue, Tamotsu Kiyoshima, Keigo Yoshizaki, Chihiro Nakatomi, Mitsushiro Nakatomi, Hayato Ohshima, Masashi Shin, Jing Gao, Kanji Tsuru, Koji Okabe, Ichiro Nakamura, Hiroaki Honda, Miho Matsuda, Ichiro Takahashi, Eijiro Jimi
Summary: p130Cas plays a crucial role in amelogenesis, as its deficiency leads to hypomineralization of enamel, abnormal structure, and impaired functionality.
Article
Biochemistry & Molecular Biology
Ayaka Yanagida, Elena Corujo-Simon, Christopher K. Revell, Preeti Sahu, Giuliano G. Stirparo, Irene M. Aspalter, Alex K. Winkel, Ruby Peters, Henry De Belly, Davide A. D. Cassani, Sarra Achouri, Raphael Blumenfeld, Kristian Franze, Edouard Hannezo, Ewa K. Paluch, Jennifer Nichols, Kevin J. Chalut
Summary: This study investigates the mechanical basis of spatial segregation between the primitive endoderm and the epiblast. It proposes that differential surface fluctuations and cellular fluidity play a crucial role in ensuring physical lineage sorting.
Article
Multidisciplinary Sciences
Nagham Khouri-Farah, Qiuxia Guo, Kerry Morgan, Jihye Shin, James Y. H. Li
Summary: Recent studies using single-cell RNA-sequencing have identified cellular heterogeneity in the developing mammalian cerebellum, but the regulatory logic behind this diversity remains unclear. Researchers integrated single-cell RNA and ATAC analyses to understand the developmental trajectories of cerebellar progenitors and identified potential regulatory elements controlling cell state transitions. Using gene regulatory network analysis, they uncovered the molecular control of a specific progenitor zone and demonstrated its association with a common cerebellar birth defect in humans.
Article
Cell Biology
Eun-Jung Kim, Han Ngoc Mai, Dong-Joon Lee, Ka-Hwa Kim, Seung-Jun Lee, Han-Sung Jung
Summary: In this study, various factors were precisely modulated to facilitate differentiation of hiPSCs into DECs, which are essential for the regeneration of functional teeth. The differentiation protocol provides a practical approach for applying hiPSCs for tooth regeneration, showcasing the potential for future regenerative dentistry applications.
CELL AND TISSUE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Batoul Chouaib, Pierre-Yves Collart-Dutilleul, Nicolas Blanc-Sylvestre, Richard Younes, Csilla Gergely, Cedric Raoul, Frederique Scamps, Frederic Cuisinier, Olivier Romieu
Summary: Stem cell-derived conditioned media show promise as pharmaceutical products for tissue regeneration, with human dental pulp cells (DPCs) conditioned medium enhancing neurite outgrowth in sensory neurons. Frozen storage does not affect activity, and 48 hours of conditioning is optimal. The addition of B-27 supplement enhances the regenerative effect of DPC secretome.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Medicine, Research & Experimental
Tong Yu, Yong Cui, Shuning Xin, Yunmei Fu, Yan Ding, Liying Hao, Hongguang Nie
Summary: Acute lung injury (ALI) is a progressive inflammatory injury, and mesenchymal stem cells (MSCs) can be used as a treatment for ALI. This study explores the protective effects of MSCs secreted keratinocyte growth factor (KGF) on ALI, and investigates the involvement of epithelial sodium channel (ENaC) in alveolar fluid reabsorption. The results show that MSC-conditioned medium (MSC-CM) and KGF can alleviate inflammation-related pulmonary edema in ALI mice, improve proliferation and decrease differentiation of alveolar type II epithelial (ATII) cells, and reverse the inhibited expression of ENaC caused by lipopolysaccharides (LPS). The study suggests that the therapeutic effect of MSC-CM on edematous ALI is closely related to KGF, and KGF may be involved in the proliferation and differentiation of ATII cells and the upregulation of ENaC expression by inhibiting the NF-kappa B signaling pathway.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Christophe Caneparo, Stephane Chabaud, Julie Fradette, Stephane Bolduc
Summary: Growing demand in various industries has led to an increase in in vitro cell cultures. However, the use of fetal bovine serum comes with challenges such as unknown constitution, potential contamination, and limited production. A Serum-Free Medium (SFM) has been developed to address these issues. The study showed that SFM enables cell proliferation and is more suitable for epithelial cells compared to the traditional medium. However, further optimization is needed for fibroblast proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Fabricio Passador-Santos, Catarina Rodrigues Rosa de Oliveira, Lucas Novaes Teixeira, Cecilia Pedroso Turssi, Rui Barbosa de Brito-Junior, Andresa Borges Soares, Nadir Severina de Freitas, Ney Soares de Araujo, Vera Cavalcanti de Araujo
Summary: This study investigates the differentiation of ameloblastic-like cells and the nature of the secreted eosinophilic materials in adenomatoid odontogenic tumors. The differentiation of rosette cells into ameloblastic-like cells is likely due to induction between these cells. Collagen I is secreted by well-differentiated ameloblastic-like cells, while some eosinophilic materials in lace-like areas may be produced by odontogenic cuboidal epithelial or intermediate stratum-like cells.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Fahimeh Safaeinejad, Sareh Asadi, Shiva Ghafghazi, Hassan Niknejad
Summary: Multiple sclerosis is a chronic inflammatory and neurodegenerative disease of the central nervous system, and current treatments focus on anti-inflammatory approaches. Amniotic epithelial stem cells show promise as a potential therapy for multiple sclerosis due to their neuroprotective and neurogenic properties.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Oncology
Jordi Canals, Alfons Navarro, Cristina Vila, Josep M. Canals, Tania Diaz, Melissa Acosta-Plasencia, Corali Cros-Font, Bing Han, Yangyi He, Mariano Monzo
Summary: This study is the first to report that stimuli from the embryonic lung can modulate the malignant phenotype of lung cancer cells, control their growth capacity, and activate their differentiation into myofibroblasts. These findings could lead to new strategies for lung cancer management.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Cell Biology
Saber Miraki, Asrin Rashidi, Omid Banafshi, Masoud Alasvand, Fardin Fathi, Mohammad Ghasem Golmohammadi
Summary: The effects of different culture media on the development stages of embryos were investigated, and it was found that the embryos have different requirements at different stages.
Article
Cell & Tissue Engineering
Selin Onen, Ali Can Atik, Merve Gizer, Sevil Kose, Onder Yaman, Haluk Kulah, Petek Korkusuz
Summary: A new microfluidic device based on bone marrow-derived mesenchymal stem cells has been found to support the maintenance of stem cells and in vitro spermatogenesis, offering potential protection and expansion of the stem cell pool for future fertility preservation in pediatric cancer survivors.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Multidisciplinary Sciences
Yaofeng Wang, Yifan Zhao, Shubin Chen, Xiaoming Chen, Yanmei Zhang, Hong Chen, Yuansong Liao, Jiashu Zhang, Di Wu, Hongxing Chu, Hongying Huang, Caixia Wu, Shijuan Huang, Huichao Xu, Bei Jia, Jie Liu, Bo Feng, Zhonghan Li, Dajiang Qin, Duanqing Pei, Jinglei Cai
Summary: In this study, the spatiotemporal relationships during odontogenesis were explored using single cell RNA sequencing and chromatin assays. The researchers traced cell trajectories and created a cell lineage atlas of developing mouse teeth. They identified specific transcriptome profiles of mesenchymal cells and key gene regulatory networks involved in tooth and bone formation. Additionally, they discovered a novel tissue called Plac8 with the ability to induce tooth regeneration.
Article
Pharmacology & Pharmacy
Lite Ge, Cheng Zhang, Huali Xie, Yi Zhuo, Chengfeng Xun, Ping Chen, Zhiping Hu, Ming Lu
Summary: Protein phosphorylation plays a crucial role in signaling pathways regulating NSC differentiation induced by OECs. The study identified the dynamics of the proteome and phosphoproteome during the early stages of NSC differentiation, highlighting the key role of TGF-beta 3 protein secreted by OECs. These findings contribute to the understanding of the regulatory network for neural lineage commitment of NSCs induced by OECs.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Batoul Chouaib, Frederic Cuisinier, Pierre-Yves Collart-Dutilleul
Summary: Conditioned medium (CM) produced from dental mesenchymal stem cells (DMSC-CM) is emerging as a potential alternative to cell therapy. However, there is a lack of standardized and optimized manufacturing procedures due to variable protocols, leading to inconsistent results.
WORLD JOURNAL OF STEM CELLS
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)