Article
Chemistry, Multidisciplinary
Prashant Jain, Chethan D. Shanthamurthy, Shani Leviatan Ben-Arye, Sharon Yehuda, Sharvani S. Nandikol, Hirekodathakallu Thulasiram, Vered Padler-Karavani, Raghavendra Kikkeri
Summary: A potential heparan sulfate (HS) ligand was discovered to target several growth factors using specific HS tetrasaccharide ligands. One tetrasaccharide ligand showed strong binding with the VEGF(165) growth factor, and in vitro experiments demonstrated the therapeutic potential of these synthetic HS ligands by inhibiting vascular endothelial cell proliferation, migration, and angiogenesis in the presence of VEGF(165) and the specific tetrasaccharides.
CHEMICAL COMMUNICATIONS
(2021)
Review
Cell Biology
Jian Liu, Lars C. Pedersen
Summary: This article summarizes the recent progress in substrate specificity studies of different 3-O-sulfotransferase isoforms and introduces a newly developed method for analyzing the level of 3-O-sulfated heparan sulfate using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Physiology
Cassandra J. Field, Alexis M. Perez, Taryn Samet, Victoria Ricles, M. Kathryn Iovine, Linda J. Lowe-Krentz
Summary: Tmem184a plays a critical role in the proper formation of blood vessels in zebrafish embryos. It acts synergistically with Vegfr2b to modulate angiogenesis and affects endothelial cell proliferation and VE-cadherin expression.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Imlirenla Pongener, Conor O'Shea, Hannah Wootton, Michael Watkinson, Gavin J. Miller
Summary: This review focuses on the chemical approaches and methodology improvements for the synthesis of heparin and heparan sulfate since 2015, including advances in accessing the pentasaccharide anticoagulant and targets for heparan sulfate synthesis. It also discusses key building block synthesis, oligosaccharide construction, chemical sulfation techniques, and technological improvements to traditional solution-phase synthesis approaches.
Article
Biochemistry & Molecular Biology
Aleen Al Halawani, Lea Abdulkhalek, Suzanne M. Mithieux, Anthony S. Weiss
Summary: The study showed that the pro-angiogenic effects of tropoelastin are mediated indirectly through its action on co-cultured cells, reversing its inhibitory effect on endothelial tube formation. In vitro experiments demonstrated that tropoelastin can mimic its known in vivo pro-angiogenic effects, highlighting its value as an indirect mediator of angiogenesis.
Article
Pharmacology & Pharmacy
Sasikarn Looprasertkul, Amornpun Sereemaspun, Nakarin Kitkumthorn, Kanidta Sooklert, Tewarit Sarachana, Depicha Jindatip
Summary: The study revealed the effects of AuNPs on pericytes, showing inhibition of proliferation, decreased migration, and reduced PDGFR-β mRNA expression. Ultrastructural analysis showed the presence of AuNPs in late endosomes, autolysosomes, and damaged mitochondria in pericytes. In 3D capillary tube formation, AuNPs led to abnormal positioning of pericytes, resulting in incomplete tube formation.
Article
Biochemistry & Molecular Biology
Jesper Bergwik, Amanda Kristiansson, Jorgen Larsson, Simon Ekstrom, Bo Akerstrom, Maria Allhorn
Summary: A1M can bind to heparin and HS, and can be purified from human plasma using heparin affinity chromatography and size exclusion chromatography. The binding strength is inversely proportional to salt concentration and directly proportional to the sulfation degree of heparin and HS.
Article
Biochemistry & Molecular Biology
Angela Boyce, Gary Walsh
Summary: Heparinases are enzymes that selectively cleave heparin and heparan sulfate chains, with potential therapeutic applications in cancer treatment, inhibition of neovascularization, and other biological processes. Their industrial and clinical significance has been underscored by their ability to produce low molecular weight heparin and neutralize heparin in blood.
Article
Biochemistry & Molecular Biology
Chara E. S. Spanou, Alexander P. Wohl, Sandra Doherr, Annkatrin Correns, Niklas Sonntag, Steffen Luetke, Matthias Morgelin, Thomas Imhof, Jan M. Gebauer, Ulrich Baumann, Kay Grobe, Manuel Koch, Gerhard Sengle
Summary: Bone morphogenetic proteins (BMPs) play important roles in regulating cellular processes. The molecular requirements for the bioavailability of BMPs in the extracellular matrix (ECM) are not fully understood. This study shows that specific interactions of the BMPs with glycosaminoglycans (GAGs) allow for their spatial concentration in a biologically active conformation. The findings also suggest that the interaction between BMPs and GAGs is specific to certain BMPs and can protect BMPs from inactivation.
Review
Biochemistry & Molecular Biology
Mingjia Yu, Tianji Zhang, Wei Zhang, Qianyun Sun, Hongmei Li, Jin-ping Li
Summary: The infectious disease caused by SARS-CoV-2 has become a major threat with high mortality rates and rapid spread, leading to over 70 million infections and 1.6 million deaths. New strategies are urgently needed for treatment due to the lack of effective therapeutics or widely available vaccines. The use of heparin/HS and their mimetics may serve as potential drugs by competing with cell surface HS to prevent viral adhesion and modulate inflammatory reactions, highlighting the importance of understanding coronavirus invasion mechanisms and HS-protein interactions for potential treatment avenues.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Medicine, Research & Experimental
Simone R. Potje, Tiago J. Costa, Thais F. C. Fraga-Silva, Ronaldo B. Martins, Maira N. Benatti, Carlos E. L. Almado, Keyla S. G. de Sa, Vania L. D. Bonato, Eurico Arruda, Paulo Louzada-Junior, Rene D. R. Oliveira, Dario S. Zamboni, Christiane Becari, Maria Auxiliadora-Martins, Rita C. Tostes
Summary: Plasma from COVID-19 patients promotes glycocalyx shedding and redox imbalance in endothelial cells, and heparin treatment potentially inhibits glycocalyx disruption.
Article
Biochemistry & Molecular Biology
Samuel G. Holmes, Umesh R. Desai
Summary: Although molecular docking remains challenging for glycosaminoglycans (GAGs) due to their flexibility and anionic character, using a semi-rigid docking protocol can better reproduce the native topology of heparin/heparan sulfate (Hp/HS). Rigid and semi-rigid protocols are more accurate in predicting longer chain poses, while the semi-rigid approach is better for 2-4-mer poses. The new semi-rigid protocol, combined with a new computational parameter, shows promising potential for high-throughput screening of GAG sequences and identifying drug targets and drug-like Hp/HS sequences.
Article
Biotechnology & Applied Microbiology
Bryan E. Thacker, Kristen J. Thorne, Colin Cartwright, Jeeyoung Park, Kimberly Glass, Annie Chea, Benjamin P. Kellman, Nathan E. Lewis, Zhenping Wang, Anna Di Nardo, Susan T. Sharfstein, Walter Jeske, Jeanine Walenga, John Hogwood, Elaine Gray, Barbara Mulloy, Jeffrey D. Esko, Charles A. Glass
Summary: Heparin, an essential anticoagulant, is currently sourced from vulnerable animal populations. This study demonstrates the feasibility of producing high anticoagulant potency heparin sulfate through genome engineering in cell culture, providing a potential alternative to animal sources.
METABOLIC ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Yi-En Liao, Jian Liu, Katelyn Arnold
Summary: Heparan sulfates and heparan sulfate binding proteins play crucial roles in regulating vascular homeostasis and host response in sepsis. Dysregulation of these molecules can exacerbate inflammation and coagulation. Understanding the structure-function relationship between heparan sulfates and heparan sulfate binding proteins is essential for drug development and potential therapeutic interventions in sepsis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lauren A. Gandy, Ashley J. Canning, Huan Lou, Ke Xia, Peng He, Guowei Su, Tina Cairns, Jian Liu, Fuming Zhang, Robert J. Linhardt, Gary Cohen, Chunyu Wang
Summary: This study investigates the interaction between glycoprotein D (gD) and neuronal surface glycan heparan sulfate (HS) from both the protein and glycan perspective. The results reveal that the N-terminal region of gD is not crucial for heparin binding, while the conformational dynamics of the C-terminal region modulate the receptor binding. Additionally, gD shows a strong preference for 6-O-sulfate and the importance of 2-O-sulfation increases in the presence of 6-O-S.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Rio S. Boothello, Aurijit Sarkar, Vy My Tran, Thao Kim Nu Nguyen, Nehru Viji Sankaranarayanan, Akul Y. Mehta, AlHumaidi Alabbas, Spencer Brown, Alessandro Rossi, April C. Joice, Caitlin P. Mencio, Maritza V. Quintero, Balagurunathan Kuberan, Umesh R. Desai
ACS CHEMICAL BIOLOGY
(2015)
Article
Neurosciences
Holly A. Holman, Vy M. Tran, Mausam Kalita, Lynn N. Nguyen, Sailaja Arungundram, Balagurunathan Kuberan, Richard D. Rabbitt
JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
(2016)
Article
Radiology, Nuclear Medicine & Medical Imaging
Kieran Murphy, Gavin Elias, Jim Steppan, Chett Boxley, Kuberan Balagurunathan, Xylophone Victor, Thomas Meaders, Mario Muto
JOURNAL OF VASCULAR AND INTERVENTIONAL RADIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Indu Bhushan, Alhumaidi Alabbas, Balagurunathan Kuberan, Ram B. Gupta, Umesh R. Desai
Article
Neurosciences
Caitlin Mencio, Balagurunathan Kuberan, Franz Goller
JOURNAL OF NEUROPHYSIOLOGY
(2017)
Article
Multidisciplinary Sciences
Jie Shi Chua, Vy M. Tran, Mausam Kalita, Maritza V. Quintero, Orlando Antelope, Geethu Muruganandam, Yukio Saijoh, Balagurunathan Kuberan
Article
Biochemical Research Methods
Diem-Trang Tran, Tian Zhang, Ryan Stutsman, Matthew Might, Umesh R. Desai, Balagurunathan Kuberan
Article
Cardiac & Cardiovascular Systems
Brett Ronald Cutler, Samira Gholami, Jie Shi Chua, Balagurunathan Kuberan, Pon Velayutham Anandh Babu
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Simon J. Foulcer, Courtney M. Nelson, Maritza V. Quintero, Balagurunathan Kuberan, Jonathan Larkin, Maria T. Dours-Zimmermann, Dieter R. Zimmermann, Suneel S. Apte
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Chemistry, Multidisciplinary
Mausam Kalita, Sivasai Balivada, Vimal Paritosh Swarup, Caitlin Mencio, Karthik Raman, Umesh R. Desai, Deryl Troyer, Balagurunathan Kuberan
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2014)
Article
Immunology
Cheryl Collins, Yuan Lui, Ana Mafalda Santos, Bryan A. Ballif, Anisha Mahalya Gogerly-Moragoda, Heather Brouwer, Robin Ross, Kuberan Balagurunathan, Sumana Sharma, Gavin J. Wright, Simon Davis, Ralph C. Budd
JOURNAL OF IMMUNOLOGY
(2019)
Review
Chemistry, Multidisciplinary
Jie Shi Chua, Balagurunathan Kuberan
ACCOUNTS OF CHEMICAL RESEARCH
(2017)
Article
Developmental Biology
Caitlin P. Mencio, Vy My Tran, Maritza V. Quintero, Mamoru Koketsu, Franz Goller, Balagurunathan Kuberan
DEVELOPMENTAL NEUROBIOLOGY
(2017)
Article
Chemistry, Medicinal
Caitlin P. Mencio, Dinesh R. Garud, Yosuke Doi, Yiling Bi, Hariprasad Vankayalapati, Mamoru Koketsu, Balagurunathan Kuberan
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2017)
Article
Chemistry, Medicinal
Yiling Bi, Matthew Might, Hariprasad Vankayalapati, Balagurunathan Kuberan
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2017)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
