Article
Cell Biology
Akos Farkas, Henning Urlaub, Katherine E. Bohnsack, Blanche Schwappach
Summary: The study reveals the important role of the guided entry of tail-anchored proteins (GET) pathway in lipotoxicity and uncovers the hairpin membrane protein Erg1 as a non-canonical GET pathway client. The GET pathway is particularly important for the acute upregulation of Erg1 under low sterol conditions, and several other proteins anchored to the endoplasmic reticulum via a hairpin are also identified as potential GET clients.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biology
Federico Buonanno, Francesco Trenti, Gabriele Achille, Adriana Vallesi, Graziano Guella, Claudio Ortenzi
Summary: This study investigates the defensive functions adopted by the ciliate Stentor polymorphus against predators in the same aquatic ecosystem. The defensive strategy of this ciliate is primarily chemical, mediated by a mix of sterols.
Article
Cell & Tissue Engineering
Duo Wang, Chang Liu, Huan Liu, Yilei Meng, Fang Lin, Yanqiong Gu, Hanrui Wang, Mengyue Shang, Chang Tong, Agapios Sachinidis, Qilong Ying, Li Li, Luying Peng
Summary: The interaction between ERG1 and Integrin beta 1 in rESCs activates the AKT pathway, leading to cardiomyocyte differentiation through multiple mechanisms including upregulation of beta-catenin and Gata4 expression and inhibition of cell apoptosis via nuclear translocation of nuclear factor-kappa B.
Review
Neurosciences
Francisco G. Sanchez-Conde, Eric N. Jimenez-Vazquez, David S. Auerbach, David K. Jones
Summary: The ERG1 potassium channel, encoded by KCNH2, is not only associated with cardiac electrical excitability but also plays a physiological regulatory role in various parts of the human body, particularly the brain. Understanding the structure, function, and relationship of ERG1 with neuronal diseases has advanced, although there are still key questions that remain unanswered.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Homa Nath Sharma, Jonathan Catrett, Ogechi Destiny Nwokeocha, Melissa Boersma, Michael E. Miller, Audrey Napier, Boakai K. Robertson, Daniel A. Abugri
Summary: Toxoplasma gondii (T. gondii) infection is increasing globally with significant socioeconomic and public health challenges. Current treatments for T. gondii infection are limited in effectiveness, safety, and cost. This study aimed to evaluate the effects of a higher fungi extract on T. gondii growth and its mechanism of action. The extract was found to inhibit T. gondii growth and induce oxidative stress and mitochondrial membrane disruption. The findings suggest that the extract holds promise as a potential source of new compounds for anti-Toxoplasma gondii drugs.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Jawad M. M. Behbehani, Mohammad Irshad, Sheikh Shreaz, Maribasappa Karched
Summary: Oral candidiasis caused by Candida albicans is difficult to manage due to drug resistance. This study found that Capsaicin, an active compound of chili pepper, has potential antifungal activity against Candida species. It also synergistically enhances the antifungal activity of Fluconazole, which may help prevent resistance development and reduce drug dosage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Lan Li, Huali Xue, Yang Bi, Rui Zhang, Carelle Jimdjio Kouasseu, Qili Liu, Mina Nan, Lumei Pu, Dov Prusky
Summary: The study showed that ozone treatment effectively suppressed the growth of Fusarium sulphureum, reduced dry rot development and DAS accumulation. Ozone treatment altered the morphology of the fungus, resulting in degradation of organelles and destruction of cell membrane integrity. Additionally, ozone treatment down-regulated the expression of genes involved in ergosterol and DAS biosynthesis pathways, leading to decreased ergosterol content and DAS accumulation.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tania Jorda, Ana Martinez-Martin, Maria Teresa Martinez-Pastor, Sergi Puig
Summary: The study found that iron bioavailability and specific regulatory factors can modulate susceptibility to terbinafine, providing potential targets for new antifungal treatments.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tania Jorda, Nicolas Rozes, Maria Teresa Martinez-Pastor, Sergi Puig
Summary: Sterol synthesis is a metabolism pathway in eukaryotes which relies on iron. Fungal ergosterol biosynthesis is down-regulated under iron deficiency conditions. This study demonstrates that the iron-regulated mRNA-binding protein Cth2 and iron limitation lead to the down-regulation of three initial enzymatic steps of ergosterol synthesis (ERG1, ERG7 and ERG11) in yeast S. cerevisiae by limiting translation and decreasing mRNA levels of these specific genes.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2023)
Article
Biochemistry & Molecular Biology
Sang Hu Kim, Luke Steere, Yong-Kang Zhang, Cari McGregor, Chris Hahne, Yasheen Zhou, Chunliang Liu, Yan Cai, Haibo Zhou, Xuefei Chen, Emily Puumala, Dustin Duncan, Gerard D. Wright, Tony Liu, Luke Whitesell, Leah E. Cowen
Summary: With the increasing resistance to current agricultural fungicides, there is a need for new antifungals that target unexploited pathways. A novel series of diazaborines with potent activity against fungal plant pathogens has been identified. The mode of action was determined to be through targeting the Erg25 enzyme, which is involved in ergosterol biosynthesis in fungi. The diazaborines showed promising efficacy in models of soybean-rust and grape-rot fungal diseases.
ACS CHEMICAL BIOLOGY
(2022)
Article
Food Science & Technology
Chanin Sillapachaiyaporn, Siriporn Chuchawankul, Sunita Nilkhet, Nuntanat Moungkote, Tewarit Sarachana, Alison T. Ung, Seung Joon Baek, Tewin Tencomnao
Summary: The study revealed that Auricularia polytricha extracts have anti-inflammatory and antioxidative effects against BPA-induced neuroinflammation, regulating the occurrence of neuroinflammation through the NF-kappa B signaling pathway.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Medicinal
Min-Kyoung Shin, Fumito Sasaki, Dae-Won Ki, Nwet Nwet Win, Hiroyuki Morita, Yoshihiro Hayakawa
Summary: This study identified bioactive compounds EPO and ERG from O. gracilioides mycelia, with EPO demonstrating significant anti-cancer effects by targeting NF-B and STAT3 inflammatory pathways in 4T1 breast cancer cells.
JOURNAL OF NATURAL MEDICINES
(2021)
Article
Medicine, Research & Experimental
Siu Wah Wong-Deyrup, Xun Song, Tsz-Wai Ng, Xiu-Bin Liu, Jian-Guo Zeng, Zhi-Xing Qing, Stephen T. Deyrup, Zhen-Dan He, Hong-Jie Zhang
Summary: The ergosterol pathway is a key target for antifungal drugs, and modifying the MIC assay by adding oxidative stressor or antioxidant allows for screening new drugs that target ergosterol biosynthesis.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Jakub Suchodolski, Jakub Muraszko, Przemyslaw Bernat, Anna Krasowska
Summary: Studies have shown that lactate can reduce Candida albicans' resistance to fluconazole by affecting the expression of the ERG11 gene and ergosterol content, leading to the delocalization of the Cdr1 protein and inhibition of efflux.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Agriculture, Multidisciplinary
Shikha Tiwari, Bijendra Kumar Singh, Nawal Kishore Dubey
Summary: This review provides recent information on the toxicology, molecular and enzymatic regulatory pathways of aflatoxins in food systems, as well as the use of green nanoformulations as a sustainable approach towards their management. Understanding the role of different genes and regulatory proteins can lead to novel preventive strategies for aflatoxin detoxification and the development of aflatoxin-resistant food items. Green nanoformulations offer enhanced bio-efficacy to plant-based preservatives, addressing some of their major challenges.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Psychology, Biological
Peter J. Vollbrecht, Cameron W. Nobile, Aaron M. Chadderdon, Emily M. Jutkiewicz, Carrie R. Ferrario
PHYSIOLOGY & BEHAVIOR
(2015)
Article
Endocrinology & Metabolism
Peter J. Vollbrecht, Omar S. Mabrouk, Andrew D. Nelson, Robert T. Kennedy, Carrie R. Ferrario
Article
Behavioral Sciences
Peter J. Vollbrecht, Kathryn M. Nesbitt, Omar S. Mabrouk, Aaron M. Chadderdon, Emily M. Jutkiewicz, Robert T. Kennedy, Carrie R. Ferrario
BEHAVIOURAL BRAIN RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Bradley R. Kraemer, John P. Snow, Peter Vollbrecht, Amrita Pathak, William M. Valentine, Ariel Y. Deutch, Bruce D. Carter
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Peter J. Vollbrecht, Linda D. Simmler, Randy D. Blakely, Ariel Y. Deutch
JOURNAL OF NEUROCHEMISTRY
(2014)
Article
Behavioral Sciences
Y. Alonso-Caraballo, K. J. Hodgson, S. A. Morgan, C. R. Ferrario, P. J. Vollbrecht
BEHAVIOURAL BRAIN RESEARCH
(2019)
Article
Neurosciences
Andrew J. Gall, Peter J. Vollbrecht, Tristan Tobias
EUROPEAN JOURNAL OF NEUROSCIENCE
(2020)
Article
Education, Scientific Disciplines
Peter J. Vollbrecht, Kirsten A. Porter-Stransky, Wendy L. Lackey-Cornelison
ADVANCES IN PHYSIOLOGY EDUCATION
(2020)
Article
Biochemistry & Molecular Biology
Peter J. Vollbrecht, Kathryn M. Nesbitt, Victoria M. Addis, Keenan M. Boulnemour, Daniel A. Micheli, Kendall B. Smith, Darleen A. Sandoval, Robert T. Kennedy, Carrie R. Ferrario
Summary: Obesity is a major health concern in the US, and inherent differences in the function of the brain's motivation centers may contribute to obesity. This study found that there are significant differences in NAc glucose utilization between obesity-prone and obesity-resistant rats, with the former favoring glutamate production over GABA. These differences are related to reduced astrocytic recycling of glutamate in the NAc.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Education, Scientific Disciplines
Kirsten A. Porter-Stransky, Peter J. Vollbrecht, David R. Riddle
Summary: A case-based laboratory event was incorporated into a pre-clerkship psychiatry-based course for second-year medical students to help them integrate foundational neurology, neuroanatomy, and psychiatry concepts by making interdisciplinary connections among these fields through working on different cases at lab stations.
MEDICAL SCIENCE EDUCATOR
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)