Article
Neurosciences
Meghyn A. Welch, Leslie-Anne R. Jansen, Deborah J. Baro
Summary: The study found that SUMOylation of Kv4.2 at K579 regulates TC internalization by promoting channel recycling. Additionally, there is a reciprocity between Kv4.2 SUMOylation and the Kv4.2 interactome such that SUMOylation regulates the interactome and the interactome influences the pattern and effect of SUMOylation.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Gastroenterology & Hepatology
Fengru Jiang, Sumei Li, Xiaoyun Wang, Yingzhao Deng, Shuying Peng
Summary: The study reveals the regulatory role of DPP10-AS1/miR-324-3p/CLDN3 axis in pancreatic cancer, suggesting DPP10-AS1 ablation as a therapeutic target against pancreatic cancer.
Article
Endocrinology & Metabolism
Alexandra Efthymiadou, Ivan Gautschi, Miguel Xavier van Bemmelen, Amalia Sertedaki, Aristeidis Giannakopoulos, George Chrousos, Laurent Schild, Dionisios Chrysis
Summary: This study investigates the genetic cause of a mild and transient form of pseudohypoaldosteronism type 1 (PHA1). It is found that all 12 patients with PHA1 carry a homozygous missense mutation p.Phe226Cys in the a subunit of ENaC, which leads to a reduction in ENaC activity and explains the symptoms in these patients.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2023)
Article
Dermatology
Miriam Armanious, Mohn'd AbuHilal
Summary: This study reports and describes clinical and histologic characteristics of 10 cases of gliptin-induced BP. All patients showed significant improvement after withdrawal of gliptin medications and proper medical treatment, with no evidence of relapse after 4 months of follow-up.
JOURNAL OF CUTANEOUS MEDICINE AND SURGERY
(2021)
Article
Cell Biology
Sara A. Kirolos, Richard H. Gomer
Summary: The ability of cells to sense chemical gradients is crucial for various biological processes, but the mechanism of chemorepulsion is poorly understood. This study reveals that Dictyostelium cells secrete a chemorepellent protein called AprA, which inhibits local Ras protein activation through multiple protein pathways, resulting in biased movement of cells away from AprA.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Biochemistry & Molecular Biology
Zahra Setayesh-Mehr, Mahdiye Poorsargol
Summary: The study showed that peptides HL-7 and HL-10 had similar effects to positive controls on glucose uptake and cell proliferation, and increased insulin secretion in a dose-dependent manner. Inhibitory assay results demonstrated competitive behavior of the peptides against DPPIV enzyme activity, indicating their potential in increasing insulin secretion through pancreatic beta cell proliferation and inhibiting DPPIV enzyme.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2021)
Article
Electrochemistry
Emad M. Hussien, Mohamed Rizk, Heba Y. Mohamed, Mona S. Elshahed
Summary: A novel screen printed graphite ion-selective electrode was developed for rapid and accurate determination of linagliptin in pure form and pharmaceutical formulation. The electrode exhibited high selectivity, fast response, and wide pH range. Results showed that the electrode had high precision and accuracy in analysis.
ANALYTICAL & BIOANALYTICAL ELECTROCHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
M. Amin, C. Gragnoli
Summary: Polycystic ovarian syndrome (PCOS) is a complex condition affecting women of reproductive age, associated with increased cardiovascular morbidity and mortality. A GW-linkage and linkage disequilibrium study in genetically homogeneous Italian families with PCOS identified several novel risk variants, genes, and pathways potentially involved in the pathogenesis of PCOS.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2023)
Article
Cell Biology
Kensuke Fujii, Koichi Nakajo, Yoshihiro Egashira, Yasuhiro Yamamoto, Kazuya Kitada, Kohei Taniguchi, Masaru Kawai, Hideki Tomiyama, Koichi Kawakami, Kazuhisa Uchiyama, Fumihito Ono
Editorial Material
Physiology
Yoshihiro Kubo
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2020)
Correction
Cell Biology
Kensuke Fujii, Koichi Nakajo, Yoshihiro Egashira, Yasuhiro Yamamoto, Kazuya Kitada, Kohei Taniguchi, Masaru Kawai, Hideki Tomiyama, Koichi Kawakami, Kazuhisa Uchiyama, Fumihito Ono
Article
Biology
Rizki Tsari Andriani, Yoshihiro Kubo
Summary: The gating of ATP-activated channel P2X2 is not only dependent on ATP concentration, but also membrane voltage. Using voltage-clamp fluorometry technique, this study observed rapid and linearly voltage-dependent fluorescence intensity changes in the TM2 domain of rat P2X2, reflecting structural rearrangements. The interaction between specific residues within the electric field is critical for voltage-dependent gating.
Article
Biochemistry & Molecular Biology
Kiichi Hirazawa, Michihiro Tateyama, Yoshihiro Kubo, Takushi Shimomura
Summary: TPCs are voltage-gated cation channels shaped by single polypeptides with two repeats of a canonical 6-transmembrane unit. These channels are regulated by physiological signals such as membrane voltage and PI, with PI binding to the first repeat affecting the movement of the distally located second S4 helix in the second repeat. This indicates a tight interrepeat coupling within TPCs, suggesting a conserved feature among TPC family members to integrate various physiological signals.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Neurosciences
I-Shan Chen, Jodene Eldstrom, David Fedida, Yoshihiro Kubo
Summary: G-protein-gated inwardly rectifying K+ (GIRK; Kir3.x) channels play important roles in controlling cell excitation in various organs. Disease-associated mutations in these channels can lead to loss of K+ selectivity, with the G156S mutation showing a unique ion permeation pathway compared to other mutants.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Editorial Material
Neurosciences
Yoshihiro Kubo
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Multidisciplinary Sciences
Yoshiaki Kise, Go Kasuya, Hiroyuki H. Okamoto, Daichi Yamanouchi, Kan Kobayashi, Tsukasa Kusakizako, Tomohiro Nishizawa, Koichi Nakajo, Osamu Nureki
Summary: Mechanisms of modulation of Kv4 channel complexes by auxiliary subunits are central to the physiological function of channels in the brain and heart. The structures of Kv4.2-KChIP1 and Kv4.2-DPP6S complexes reveal that KChIP1 prevents N-type inactivation and stabilizes the S6 conformation, while DPP6S interacts with the S1 and S2 helices to accelerate voltage-dependent movement. KChIP1 and DPP6S do not directly interact with each other in the ternary complex, suggesting two distinct modes of modulation contribute to evoke A-type currents.
Article
Physiology
Niklas Brake, Adamo S. Mancino, Yuhao Yan, Takushi Shimomura, Yoshihiro Kubo, Anmar Khadra, Derek Bowie
Summary: By combining electrophysiology and kinetic modeling, Brake et al. found that closed-state inactivation plays a significant role in the gating behavior of cardiac, skeletal muscle, and neuronal voltage-gated sodium channels. The study showed that both VSD-III and VSD-IV are necessary for the inactivation of the cardiac sodium channel, Nav1.5, and that all VSDs can modulate steady-state inactivation. The researchers also demonstrated that channel activation is partially determined by the movement of VSD-IV.
JOURNAL OF GENERAL PHYSIOLOGY
(2022)
Article
Physiology
Jiaying Liu, Go Kasuya, Buntaro Zempo, Koichi Nakajo
Summary: The HCN4 channel is crucial for heart rate regulation, and abnormalities can lead to heart diseases. Zebrafish, a suitable animal model for cardiovascular research, has different HCN4 channels compared to humans. This study characterized the physiological properties of zebrafish HCN4 channels and found their significant roles in heart rate regulation and sensitivity to inhibitors.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Takushi Shimomura, Kiichi Hirazawa, Yoshihiro Kubo
Summary: This study reveals that different conformations of DII-S4 determine the gating modes in Two-pore channels (TPCs), with the intermediate state representing the PIP2-gating mode. TPC3 is primarily voltage-dependent, while TPC2 is PIP2-gated and nonvoltage-dependent. Furthermore, the antidepressant desipramine induces voltage dependence in TPC2, and the flavonoid naringenin biases the mode preference from PIP2-gating to desipramine-induced voltage gating.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Go Kasuya, Koichi Nakajo
Summary: In this study, the researchers investigated the interaction between KCNE3 and KCNQ1 voltage sensors in the KCNQ1-KCNE3-calmodulin complex. They found that the distance between the S1 segment and KCNE3 is optimized to achieve constitutive activity. In addition, they identified mutant pairs of KCNQ1 and KCNE3 that partially restored constitutive activity when expressed together.
Editorial Material
Neurosciences
Yoshihiro Kubo, Peter Kohl
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Multidisciplinary Sciences
Shogo Hori, Michihiro Tateyama, Tsuyoshi Shirai, Yoshihiro Kubo, Osamu Saitoh
Summary: The authors discovered that the heat activation threshold of TRPV1 in tailed amphibians is reduced by substitution of two specific amino acids in the N-terminal region, suggesting their role in cool-habitat selection.
NATURE COMMUNICATIONS
(2023)
Review
Biophysics
Koichi Nakajo
BIOPHYSICS AND PHYSICOBIOLOGY
(2019)