Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 402, Issue 4, Pages 595-601Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.10.059
Keywords
Bitter; Sweet; Taste; Calcium imaging; Gentiobiose; Binding site
Categories
Funding
- Research and Development Program for New Bio-industry Initiatives of Bio-oriented Technology Research Advancement Institution (BRAIN)
- Ministry of Education Culture Sports Science and Technology of Japan [20780089, 22688010, 20688015, 21658046, 22380072, 22300256, 20380183]
- Grants-in-Aid for Scientific Research [22380072, 22300256, 20780089, 22688010, 21658046] Funding Source: KAKEN
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Sweetness and bitterness are key determinants of food acceptance and rejection respectively Sugars such as sucrose and fructose are generally recognized as sweet However not all sugars are sweet and even anomers may have quite different tastes For example gentiobiose is bitter whereas its anomer isomaltose is sweet Despite this unique sensory character the molecular basis of the bitterness of gentiobiose remains to be clarified In this study we used calcium imaging analysis of human embryonic kidney 293T cells that heterologously expressed human taste receptors to demonstrate that gentiobiose activated hTAS2R16 a bitter taste receptor but not hT1R2/hT1R3 a sweet taste receptor In contrast isomaltose activated hT1R2/hT1R3 As a result these anomers elicit different taste sensations Mutational analysis of hTAS2R16 also indicated that gentiobiose and beta-D-glucopyranosides such as salicin share a common binding site of hTAS2R16 (C) 2010 Elsevier Inc All rights reserved
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