Review
Biochemistry & Molecular Biology
Miklos Lengyel, Peter Enyedi, Gabor Czirjak
Summary: K-2P subunits form leak potassium channels with constitutive activity, including the mechanosensitive TREK subfamily. These channels open in response to mechanical force, leading to hyperpolarization and inhibition of cellular functions. The TREK subfamily is among the best-understood examples of mammalian ion channels influenced by phospholipid bilayer tension, crucial for regulating membrane potential in various cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Rachael M. Vacassenno, Christine N. Haddad, Robin L. Cooper
Summary: The resting membrane potential of cells is maintained by potassium K2p channels. The function and expression profiles of K2p channel subtypes in the Drosophila genome have not yet been determined. Doxapram depolarizes the muscle and motor neurons, increases the frequency of spontaneous quantal events and evoked excitatory junction potentials, and its effects are paralleled by verapamil. These findings are significant for understanding the function of pharmacological agents that affect K2p channels and their contribution to tissue physiology using the genetically amenable model of Drosophila.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rachael M. Vacassenno, Christine N. Haddad, Robin L. Cooper
Summary: Exposure to bacterial lipopolysaccharides (LPS) hyperpolarizes the membrane potential of Drosophila skeletal muscle rapidly and transiently, but the mechanism responsible for this effect is still unclear. This study investigated the activation of calcium-activated potassium channels (K-Ca) and/or K2p channels by LPS. The results showed that 2-Aminoethyl diphenylborinate (2-APB) induced waves of hyperpolarization even during desensitization of the response to LPS, suggesting that doxapram blocked the acid-sensitive K2p tandem-pore channel subtype known in mammals. Evaluation: 9/10.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Review
Cell Biology
Felix Wiedmann, Norbert Frey, Constanze Schmidt
Summary: K-2P channels play important roles in maintaining resting membrane potential, modulating action potential repolarization, and regulating physiological processes. Dysregulation of K-2P channels in cardiovascular diseases may have therapeutic significance. Channels like K(2P)3.1 (TASK-1) and K(2P)2.1 (TREK-1) are associated with atrial fibrillation and cardiac remodeling, respectively.
Article
Biology
SeCheol Oh, Fabrizio Marinelli, Wenchang Zhou, Jooyeon Lee, Ho Jeong Choi, Min Kim, Jose D. Faraldo-Gomez, Richard K. Hite
Summary: Structures and simulations reveal the unique permeation mechanism of the human lysosomal K+ channel transmembrane protein 175 (TMEM175), which lacks the canonical K+ selectivity filter. Despite the absence of a selectivity filter, TMEM175 is selective for K+ over Na+ due to a narrow hydrophobic constriction and a favorable electrostatic field.
Article
Multidisciplinary Sciences
Linus J. Conrad, Peter Proks, Stephen J. Tucker
Summary: This passage discusses a voltage-dependent gating process in the TREK-2 K+ channel, which can be modulated by small molecule agonists and high internal K+ concentrations. It is found that high ionic-strength can inhibit the channel conductance, while the activatory effect of BL1249 on channel open probability persists under these conditions. The influence of negatively charged activators like BL1249 on permeation is consistent with an electrostatic mechanism, suggesting complexity in their effect on channel gating.
Article
Neurosciences
Xin-Shen Wu, Shobana Subramanian, Yalan Zhang, Bo Shi, Jessica Xia, Tiansheng Li, Xiaoli Guo, Lynda El-Hassar, Klara Szigeti-Buck, Jorge Henao-Mejia, Richard A. Flavell, Tamas L. Horvath, Elizabeth A. Jonas, Leonard K. Kaczmarek, Ling-Gang Wu
Summary: Potassium channels play a crucial role in endocytosis and vesicle mobilization by organizing the F-actin cytoskeleton, in addition to their traditional role in ion conductance. Mutations in potassium channels that impair these non-conducting functions may contribute to various neurological disorders.
Article
Neurosciences
Hirosato Kanda, Koichi Noguchi, Yi Dai
Summary: Saltatory conduction allows fast propagation of action potentials along myelinated nerves by utilizing the node of Ranvier. The K2P channels TREK-1 and TRAAK were found to be highly expressed in the node of Ranvier and play a crucial role in action potential repolarization. In this study, membrane stretch was shown to increase single-channel event numbers and open probability, as well as decrease membrane excitability. Blockage of TREK-1/TRAAK channels reversed these changes, suggesting their activation suppresses neuronal excitability following axonal stretch. These findings indicate that TREK-1/TRAAK channels may prevent ectopic action potential discharge during intense mechanical nerve stretch.
Review
Pharmacology & Pharmacy
Rosa Scala, Fatima Maqoud, Marina Antonacci, Jacopo Raffaele Dibenedetto, Maria Grazia Perrone, Antonio Scilimati, Karen Castillo, Ramon Latorre, Diana Conte, Said Bendahhou, Domenico Tricarico
Summary: Bisphosphonates are commonly used bone-specific anti-resorptive agents, but their effects on ion channels in musculoskeletal cells are poorly understood. Ion channels are emerging as potential targets for bisphosphonates, particularly the TRPV1 channel in osteoblasts. Further research in this area could provide insights into new therapeutic approaches for skeletal disorders.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ravikumar Reddi, Kimberly Matulef, Erika Riederer, Pierre Moenne-Loccoz, Francis Valiyaveetil
Summary: Regulation of ion conduction through the pore of a K+ channel involves coordinated action of the activation gate and inactivation gate, with the W67F mutant of the KcsA channel showing reduced inactivation and enhanced activation rate. Structural studies provide insights into the allosteric pathway and highlight the importance of ion occupancy at the S2 site for channel inactivation.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shira Burg, Shir Shapiro, Asher Peretz, Elvira Haimov, Boris Redko, Adva Yeheskel, Luba Simhaev, Hamutal Engel, Avi Raveh, Ariel Ben-Bassat, Michael Murninkas, Rotem Polak, Yoni Haitin, Yoram Etzion, Bernard Attali
Summary: Phosphatidylinositol 4-5 bisphosphate (PIP2) potently activates the SK4 channel by docking to the boundary of the CaM-binding domain. An allosteric blocker, BA6b9, inhibits SK4 channels by interacting with specific residues in the linker S4-S5, preventing proper interaction between the Ca2+-CaM N-lobe and the channel linker region. Inhibition of SK4 channels by targeting drugs to the CaM-PIP2-binding domain may serve as a promising anti-arrhythmic therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Venkata Shiva Mandala, Roderick MacKinnon
Summary: Voltage-dependent ion channels regulate the opening of their pores by sensing the membrane voltage. Cryo-electron microscopy was used to visualize the movement of S4 helix in the mammalian Eag voltage-dependent potassium channel, explaining how the voltage sensor locks the pore shut at hyperpolarizing membrane voltages.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemical Research Methods
David McCoull, Emma Ococks, Jonathan M. Large, David C. Tickle, Alistair Mathie, Jeffrey Jerman, Paul D. Wright
Summary: This study aimed to investigate the K2P superfamily using a target class approach to identify novel activators across all subclasses of K2P channels. By developing a common assay platform and screening multiple targets simultaneously, this approach allowed precise modulation of channel function for optimizing activator discovery.
Article
Biochemistry & Molecular Biology
Angel Fenollar, Albert Ros-Lucas, Maria Pia Alberione, Nieves Martinez-Peinado, Miriam Ramirez, Miguel Angel Rosales-Motos, Ling Y. Lee, Julio Alonso-Padilla, Luis Izquierdo
Summary: This study investigates the effects of specific inhibitors on the growth of the malaria parasite Plasmodium falciparum and validates metabolic pathways as potential therapeutic targets. Docking simulations provide insights into the modes of action of these inhibitors. The results highlight GPI biosynthesis and N-glycosylation pathways as promising targets for anti-malarial treatments.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Heng Ma, Xianlong Chen, Shengwei Mo, Yue Zhang, Xinxin Mao, Jingci Chen, Yilin Liu, Wei-Min Tong, Zhaohui Lu, Shuangni Yu, Jie Chen
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a highly fatal malignancy due to aberrant protein glycosylation and resistance to death. This study explores the crosstalk between glycosylation and ferroptosis in PDAC, and identifies the role of 4F2hc glycosylation in ferroptosis. Targeting the vulnerabilities of glycosylation and ferroptosis may be a potential therapeutic strategy for PDAC.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Immunology
Andreas Schulte-Mecklenbeck, Stefan Bittner, Petra Ehling, Frank Doering, Erhard Wischmeyer, Johanna Breuer, Alexander M. Herrmann, Heinz Wiendl, Sven G. Meuth, Catharina C. Gross
EUROPEAN JOURNAL OF IMMUNOLOGY
(2015)
Article
Multidisciplinary Sciences
Hariharan Subramanian, Frank Doering, Sina Kollert, Natalia Rukoyatkina, Julia Sturm, Stepan Gambaryan, Angelika Stellzig-Eisenhauer, Philipp Meyer-Marcotty, Martin Eigenthaler, Erhard Wischmeyer
Article
Pharmacology & Pharmacy
Yea Lu Tay, Yi Fan Teah, Yoong Min Chong, Mohd Fadzly Amar Jamil, Sina Kollert, Mohd Ilham Adenan, Habibah Abdul Wahab, Frank Doering, Erhard Wischmeyer, Mei Lan Tan
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Nicole Bobak, Stefan Bittner, Joseph Andronic, Susanne Hartmann, Friederike Muehlpfordt, Tilman Schneider-Hohendorf, Karen Wolf, Carsten Schmelter, Kerstin Goebel, Patrick Meuth, Heiko Zimmermann, Frank Doering, Erhard Wischmeyer, Thomas Budde, Heinz Wiendl, Sven G. Meuth, Vladimir L. Sukhorukov
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2011)
Article
Neurosciences
Maruschka Weber, Angelika Schmitt, Erhard Wischmeyer, Frank Doering
EUROPEAN JOURNAL OF NEUROSCIENCE
(2008)
Article
Biochemistry & Molecular Biology
Ralf Kleene, Claas Cassens, Robert Baehring, Thomas Theis, Mei-Fang Xiao, Alexander Dityatev, Claus Schafer-Nielsen, Frank Doering, Erhard Wischmeyer, Melitta Schachner
JOURNAL OF BIOLOGICAL CHEMISTRY
(2010)
Article
Cell Biology
Ute Renner, Andre Zeug, Andrew Woehler, Marcus Niebert, Alexander Dityatev, Galina Dityateva, Nataliya Gorinski, Daria Guseva, Dalia Abdel-Galil, Matthias Froehlich, Frank Doering, Erhard Wischmeyer, Diethelm W. Richter, Erwin Neher, Evgeni G. Ponimaskin
JOURNAL OF CELL SCIENCE
(2012)
Article
Biochemical Research Methods
Kerstin Goebel, Stefan Bittner, Tobias Ruck, Thomas Budde, Erhard Wischmeyer, Frank Doering, Heinz Wiendl, Sven G. Meuth
JOURNAL OF IMMUNOLOGICAL METHODS
(2011)
Article
Neurosciences
Michaela Eckert, Brigitte Egenberger, Frank Doering, Erhard Wischmeyer
Article
Multidisciplinary Sciences
Nadine Schaefer, Maximilian Friedrich, Morten Egevang Jorgensen, Sina Kollert, Hermann Koepsell, Erhard Wischmeyer, Klaus-Peter Lesch, Dietmar Geiger, Frank Doering
Article
Multidisciplinary Sciences
Sina Kollert, Benjamin Dombert, Frank Doering, Erhard Wischmeyer
SCIENTIFIC REPORTS
(2015)
Article
Biology
Lukas Hofmann, Dorothea Hose, Anne Griesshammer, Robert Blum, Frank Doering, Sulayman Dib-Hajj, Stephen Waxman, Claudia Sommer, Erhard Wischmeyer, Nurcan Uceyler
Article
Pharmacology & Pharmacy
Sina Kollert, Frank Doering, Ulrich Gergs, Erhard Wischmeyer
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2020)
Article
Cell Biology
Tobias Ruck, Stefanie Bock, Steffen Pfeuffer, Christina B. Schroeter, Derya Cengiz, Paul Marciniak, Maren Lindner, Alexander Herrmann, Marie Liebmann, Stjepana Kovac, Lukas Gola, Leoni Rolfes, Marc Pawlitzki, Nils Opel, Tim Hahn, Udo Dannlowski, Thomas Pap, Felix Luessi, Julian A. Schreiber, Bernhard Wuensch, Tanja Kuhlmann, Guiscard Seebohm, Bjoern Tackenberg, Patricia Seja, Frank Doering, Erhard Wischmeyer, Achmet Imam Chasan, Johannes Roth, Luisa Klotz, Gerd Meyer zu Hoerste, Heinz Wiendl, Tobias Marschall, Stefan Floess, Jochen Huehn, Thomas Budde, Tobias Bopp, Stefan Bittner, Sven G. Meuth
Summary: This study reveals how K(2P)18.1 translates TCR signals into thymic T cell fate decisions and Treg development, as well as its role in human Treg development. The findings provide a basis for therapeutic utilization of Treg in several human disorders.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)