Article
Chemistry, Analytical
Lili Zhang, Xiang Zou, Jing Huang, Jie Fan, Xiangjie Sun, Bohan Zhang, Bin Zheng, Chongyuan Guo, Deliang Fu, Lie Yao, Minbiao Ji
Summary: The study shows that label-free coherent nonlinear optical microscopy with combined SRS and SHG can reveal key diagnostic features of both normal and cancerous pancreatic tissues, providing potential for rapid intraoperative diagnosis of pancreatic cancer and predictive value for postoperative pancreatic fistula.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Chenxi Qian, Kun Miao, Li-En Lin, Xinhong Chen, Jiajun Du, Lu Wei
Summary: VISTA is a label-free superresolution method combining sample expansion and vibrational imaging, enabling high-resolution interrogation of protein-rich biological structures in cells and tissues, with a resolution down to 78 nm.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Benedetta Talone, Arianna Bresci, Francesco Manetti, Federico Vernuccio, Alejandro De La Cadena, Chiara Ceconello, Maria Lucia Schiavone, Stefano Mantero, Ciro Menale, Renzo Vanna, Giulio Cerullo, Cristina Sobacchi, Dario Polli
Summary: Bone tissue has a complex microarchitecture and biomolecular composition, which play a crucial role in determining its biomechanical properties. By utilizing multimodal nonlinear optical microscopy, it is possible to accurately characterize and compare normal and pathological bone tissue at biochemical, morphological, and topological levels. This technique has the potential to be applied in preclinical studies and even clinical practice for the analysis of larger tissue sections.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Medicine, General & Internal
Sahar Al-Shareefi, Ali Addie, Lamis Al-Taee
Summary: This study evaluated the changes in caries-invaded dentin using Raman spectroscopy and Vickers microhardness, and found that caries-infected dentin exhibited specific spectral peaks and the peaks in the amide regions varied in different carious lesions. The mineral: matrix peak ratio was correlated to the microhardness number within carious lesions.
Article
Chemistry, Analytical
Tanveer Ahmed Shaik, Enrico Baria, Xinyue Wang, Florian Korinth, Joao L. Lagarto, Christiane Hoeppener, Francesco S. Pavone, Volker Deckert, Juergen Popp, Riccardo Cicchi, Christoph Krafft
Summary: This study investigated the biochemical, structural, and biomechanical effects of genipin (GE) cross-linking on equine pericardium (EP) using optical imaging techniques and atomic force microscopy (AFM). Results showed that GE cross-linking led to a decrease in second-harmonic signal and an increase in autofluorescence, indicating changes in biochemical and structural properties. Additionally, cross-linking increased the Young's modulus of EP.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Pengcheng Wang, Hao Sun, Wei Yang, Yimin Fang
Summary: This paper reviews the application of label-free optical methods for the rapid detection of pathogenic bacteria. These methods have advantages of rapidity, simplicity, and relatively low-cost, and can overcome the limitations of traditional detection techniques, promoting precise therapy initiation.
Article
Chemistry, Analytical
Adam P. Lister, Callum J. Highmore, Niall Hanrahan, James Read, Alasdair P. S. Munro, Samuel Tan, Raymond N. Allan, Saul N. Faust, Jeremy S. Webb, Sumeet Mahajan
Summary: The current methods for diagnosis of infections are complex and time-consuming. This study presents a novel multi-excitation Raman spectroscopy-based method that can detect microbial pathogens using unprocessed clinical samples, providing rapid data for diagnosis. The method uses differential excitation of molecular components in bacterial cells to obtain strain-level distinction. The results demonstrate high accuracy in detecting respiratory pathogens associated with cystic fibrosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Halina Abramczyk, Beata Brozek-Pluska, Monika Kopec, Jakub Surmacki, Maciej Blaszczyk, Maciej Radek
Summary: This study demonstrated the impact of cancerogenesis on the redox status of mitochondrial cytochromes in human breast cancer and brain tumors using Raman spectroscopy and imaging. The correlation between cytochromes Raman bands intensity and malignancy grade was shown. Raman imaging provides additional insight into the biology of astrocytomas and breast ductal invasive cancer for noninvasive grading and differential diagnosis.
Article
Chemistry, Physical
Yu Tian, Weiqing Xu, Kongshuo Ma, Lili Cong, Yanting Shen, Xiaoxia Han, Chongyang Liang, Lijia Liang, Guohua Qi, Yongdong Jin, Shuping Xu
Summary: A novel surface-enhanced Raman scattering (SERS) strategy, based on evanescent field excitation, is proposed for label-free analysis of membrane proteins in situ. By wrapping metal nanoparticles around extracted cell membranes and utilizing leaky mode of a waveguide, high-quality SERS profiles of membrane proteins are achieved. This method has broader applicability in establishing the Ramanomics of membrane proteins and revealing exact changes during physiological processes.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Spectroscopy
Anna Pieczara, Ewelina Matuszyk, Piotr Szczesniak, Jacek Mlynarski, Malgorzata Baranska
Summary: The role of mitochondria in cellular function, including the production of reactive oxygen species and the regulation of cell death, is significant. This study utilizes Raman microscopy to locate and track functional changes in mitochondria, and explores the potential of using Raman probes for cell labeling to improve specificity and sensitivity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Review
Optics
Natan T. Shaked, Stephen A. Boppart, Lihong V. Wang, Juergen Popp
Summary: This Review provides a comprehensive overview and comparison of different label-free imaging modalities, aiming to facilitate collaboration, technological advancements, and biophysical discoveries, as well as new avenues in clinical detection, diagnosis, and monitoring of diseases.
Article
Chemistry, Inorganic & Nuclear
Roberta Sgammato, Niels Van Brempt, Roy Aerts, Sabine Van Doorslaer, Sylvia Dewilde, Wouter Herrebout, Christian Johannessen
Summary: Protoglobin from Methanosarcina acetivorans (MaPgb) is a dimeric globin belonging to the same lineage as globin-coupled sensors. It has a putative role in scavenging reactive nitrogen and oxygen species. Through various spectroscopic techniques, the unusual reaction of ferric MaPgb with nitrite was revealed. Unlike other globins, nitrite did not induce the formation of a nitriglobin form in MaPgb. Instead, it led to the formation of a stable nitric-oxide ligated ferric form of the protein (MaPgb-NO) in mildly acidic pH. The changes in the optical and vibrational properties of MaPgb upon nitric-oxide ligation provide insights into its gas-binding mechanism.
DALTON TRANSACTIONS
(2023)
Article
Microbiology
Aikaterini Pistiki, Stefan Monecke, Haodong Shen, Oleg Ryabchykov, Thomas W. Bocklitz, Petra Roesch, Ralf Ehricht, Juergen Popp
Summary: Label-free Raman spectra capture the high chemical complexity of bacterial cells. This study highlights the importance of selecting the most suitable Raman approach that can provide spectral features associated with the cell mechanism under investigation. The results show that single-cell analysis with 532 nm excitation is the preferred method for differentiating MRSA from MSSA as it captures the differences in overall biochemical composition. These findings should be considered in future studies aiming to develop label-free Raman spectroscopy as a clinical analytical tool for antimicrobial resistance determination.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra Borek-Dorosz, Anna Pieczara, Krzysztof Czamara, Marta Stojak, Ewelina Matuszyk, Katarzyna Majzner, Krzysztof Brzozowski, Arianna Bresci, Dario Polli, Malgorzata Baranska
Summary: In this study, the functionality of inflamed cells was verified by analyzing their ability to uptake and accumulate exogenous saturated fatty acids. The results showed that the uptake of fatty acids in inflamed cells is dependent on their concentration and type, providing a better understanding of endothelial cell inflammation and its impact on their fat buffering capacity. The microscopic methods used in the analysis showed complementarity in studying fatty acid uptake by endothelial cells from different perspectives.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Materials Science, Multidisciplinary
Basil G. Eleftheriades, Emily E. Storey, Amr S. Helmy
Summary: A new approach to enhance the sensitivity of spontaneous Raman spectroscopy is reported for the first time, through the formation of a thin film via thermophoresis and evaporation at the facet of a hollow-core photonic crystal fiber. This method significantly increases the detection sensitivity by more than 6 orders of magnitude, allowing for the search of trace analytes in solutions with ease.
ADVANCED OPTICAL MATERIALS
(2022)
Editorial Material
Hematology
Aaron S. Hess, John R. Hess
Article
Multidisciplinary Sciences
Tsunehisa Yamamoto, Takeo Minamikawa, Yoshinori Harada, Yoshihisa Yamaoka, Hideo Tanaka, Hitoshi Yaku, Tetsuro Takamatsu
SCIENTIFIC REPORTS
(2018)
Article
Chemistry, Physical
Yasuaki Kumamoto, Kentaro Mochizuki, Kosuke Hashimoto, Yoshinori Harada, Hideo Tanaka, Katsumasa Fujita
JOURNAL OF PHYSICAL CHEMISTRY B
(2019)
Article
Computer Science, Information Systems
Go Itami, Osamu Sakai, Yoshinori Harada
Article
Chemistry, Physical
J. Nicholas Taylor, Kentaro Mochizuki, Kosuke Hashimoto, Yasuaki Kumamoto, Yoshinori Harada, Katsumasa Fujita, Tamiki Komatsuzaki
JOURNAL OF PHYSICAL CHEMISTRY B
(2019)
Article
Biochemistry & Molecular Biology
Khalifa Mohammad Helal, James Nicholas Taylor, Harsono Cahyadi, Akira Okajima, Koji Tabata, Yoshito Itoh, Hideo Tanaka, Katsumasa Fujita, Yoshinori Harada, Tamiki Komatsuzaki
Article
Oncology
Midori Morita, Hideo Tanaka, Yasuaki Kumamoto, Akihiro Nakamura, Yoshinori Harada, Takehiro Ogata, Koichi Sakaguchi, Tetsuya Taguchi, Tetsuro Takamatsu
Article
Multidisciplinary Sciences
Tatsuya Matsumoto, Hirohiko Niioka, Yasuaki Kumamoto, Junya Sato, Osamu Inamori, Ryuta Nakao, Yoshinori Harada, Eiichi Konishi, Eigo Otsuji, Hideo Tanaka, Jun Miyake, Tetsuro Takamatsu
SCIENTIFIC REPORTS
(2019)
Article
Oncology
Tatsuya Matsumoto, Yasutoshi Murayama, Hisataka Matsuo, Kengo Okochi, Naotaka Koshiishi, Yoshinori Harada, Hideo Tanaka, Tetsuro Takamatsu, Eigo Otsuji
Article
Chemistry, Multidisciplinary
Yue Wang, Koki Ogasahara, Daisuke Tomihama, Radomir Mysliborski, Masatoshi Ishida, Yongseok Hong, Yusuke Notsuka, Yoshihisa Yamaoka, Tomotaka Murayama, Atsuya Muranaka, Masanobu Uchiyama, Shigeki Mori, Yuhsuke Yasutake, Susumu Fukatsu, Dongho Kim, Hiroyuki Furuta
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Biochemistry & Molecular Biology
Eiji Takahashi, Daisuke Yamaguchi, Yoshihisa Yamaoka
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Optics
Yusuke Notsuka, Makoto Kurihara, Nobuyuki Hashimoto, Yoshinori Harada, Eiji Takahashi, Yoshihisa Yamaoka
Summary: This study integrates adaptive optics into photoacoustic microscopy to improve the lateral resolution and depth discrimination for blood vessels in deep tissues.
Proceedings Paper
Engineering, Biomedical
Yasuaki Kumamoto, Kentaro Mochizuki, Kosuke Hashimoto, Yoshinori Harada, Hideo Tanaka, Katsumasa Fujita
BIOMEDICAL IMAGING AND SENSING CONFERENCE 2020
(2020)
Article
Optics
Takeo Minamikawa, Takashi Masuoka, Takashi Ogura, Kyuki Shibuya, Ryo Oe, Eiji Hase, Yoshiaki Nakajima, Yoshihisa Yamaoka, Takahiko Mizuno, Masatomo Yamagiwa, Yasuhiro Mizutani, Hirotsugu Yamamoto, Tetsuo Iwata, Kaoru Minoshima, Takeshi Yasui
Proceedings Paper
Microscopy
Yoshihisa Yamaoka, Yuka Kimura, Yoshinori Harada, Tetsuro Takamatsu, Eiji Takahashi
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2018
(2018)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)