Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 382, Issue 2, Pages 264-268Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.02.148
Keywords
Connexin32; Endothelial cells; Gap junctions; Cell-cell interaction; Vascular inflammation; Blood coagulation; Endothelial functions; Cx32 knock-out mice
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Funding
- Japan Society for the Promotion of Science (JSPS) [16022232, 17390276, 17790207, 18591749, 18659280, 19390262]
- Mie University COE-A Project Fund
- Okasan-Kato Foundation
- Uehara Memorial Foundation
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Endothelial cells (ECs) play many roles in vascular biology, including control of blood pressure, blood clotting, atherosclerosis, angiogenesis, and inflammation. Gap junctions (GJs) are channel-like assemblies of connexin (Cx) family proteins that connect neighboring cells and modulate and synchronize their intracellular environments by the transfer of intracellular mediators. It has been reported that vascular ECs express Cx37, Cx40, and Cx43, but not Cx32. Here, we showed that Cx32 mRNA and protein are expressed in various cultured human ECs. We confirmed Cx32 expression in blood vessel ECs using wild-type and Cx32 knock-out mice. We observed that dye transfer between cultured ECs through gap junctions is suppressed by an anti-Cx32 monoclonal antibody. These findings Suggest that vascular ECs express Cx32, which participates in endothelial gap-junction intercellular communication. (C) 2009 Elsevier Inc. All rights reserved.
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