Article
Biochemistry & Molecular Biology
Muhammad Azizan Samad, Mohd Zuwairi Saiman, Nazia Abdul Majid, Saiful Anuar Karsani, Jamilah Syafawati Yaacob
Summary: This study found that berberine can decrease telomerase activity and level in HCT 116 cells, which subsequently inhibits the proliferative ability of the cells.
Article
Biology
Alexander N. Malyavko, Olga A. Petrova, Maria Zvereva, Vladimir Polshakov, Olga A. Dontsova
Summary: This study reports the functional characterization of the Rif1 homologue from the methylotrophic thermotolerant budding yeast Hansenula polymorpha DL-1. The study reveals that Rif1 in H. polymorpha suppresses telomerase-dependent telomere elongation through direct DNA binding and association with the Ku heterodimer. It is also found that Rif1 binds Stn1 and promotes its accumulation at telomeres in H. polymorpha.
Article
Biochemistry & Molecular Biology
Neil B. Metcalfe, Mats Olsson
Summary: This review highlights the dynamic processes of ROS production, telomeric DNA damage, and DNA repair, emphasizing the trade-off between energetic efficiency and telomere protection. Mitochondrial features and ATP production efficiency play crucial roles in maintaining DNA integrity and telomere dynamics, with implications for individual variation and adaptation to changing environmental contexts.
Review
Oncology
Peter Lansdorp
Summary: The number of telomere repeats varies greatly between chromosomes, cells, and species. Loss of telomere repeats limits cell proliferation and contributes to aging, but the mechanisms behind these processes are still not fully understood. Further research is needed to investigate the role of factors such as damaged telomeric DNA, replication errors, chromatin structure, and secondary DNA structures in telomere dynamics in different cell types.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Xi Zheng, Felix Wezel, Anca Azoitei, Sabine Meessen, Wenya Wang, Gregoire Najjar, Xue Wang, Johann M. Kraus, Hans A. Kestler, Axel John, Friedemann Zengerling, Christian Bolenz, Cagatay Guenes
Summary: The telomere shortening in BC and RCC patients was significantly shorter compared to patients without malignant disease. The relative telomere length in PBL cells was associated with the survival of BC and RCC patients, making it a potential prognostic marker for these cancers.
Review
Cell Biology
Sarah W. Cai, Titia de Lange
Summary: In this review, the authors discuss the role of the CST-Polα/Primase complex in telomere length regulation. They highlight that this complex not only functions in strand synthesis but also controls telomerase to maintain telomere length. Additionally, they emphasize the importance of this complex in preventing telomeric disorders by replenishing lost sequences and determining telomere length.
GENES & DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Lucy Button, Bryony Rogers, Emily Thomas, Alice Bradfield, Rafah Alnafakh, Josephine Drury, Dharani K. Hapangama
Summary: This study aimed to explore the risk of relapse in endometrial cancer after surgery and found that telomere/telomerase associated proteins may play an important role in prognosis, particularly NHP2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Gustavo Borges, Melanie Criqui, Lea Harrington
Summary: Telomere maintenance is crucial for the integrity of both normal and cancer cells. The instability of telomeres can lead to genome instability in cancer and aging, but cancer cells can partially overcome this instability by increasing telomerase expression.
MOLECULAR ONCOLOGY
(2022)
Review
Cell Biology
Remington E. Hoerr, Katrina Ngo, Katherine L. Friedman
Summary: Telomeres are repetitive sequences located at the ends of eukaryotic chromosomes that protect chromosome ends and facilitate telomere elongation. Telomerase, a ribonucleoprotein enzyme, uses an RNA template to synthesize telomeric DNA at chromosome ends and internal non-telomeric sequences. Telomerase action at double-strand breaks can interfere with DNA repair mechanisms but may prevent nucleolytic resection and chromosome rearrangement through chromosome healing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Daniel I. Sullivan, Fiona M. Bello, Agustin Gil Silva, Kevin M. Redding, Luca Giordano, Angela M. Hinchie, Kelly E. Loughridge, Ana L. Mora, Melanie Konigshoff, Brett A. Kaufman, Michael J. Jurczak, Jonathan K. Alder
Summary: Mitochondria play important roles in cellular metabolism and signaling. Defects in mitochondria caused by genetic or acquired factors can lead to various pathologies, including premature cellular senescence. In this study, we investigated the effects of dysfunctional telomeres on mitochondrial biogenesis and function during senescence. Our results showed that senescent cells had increased mitochondrial respiratory capacity and volume. Even in vivo, hepatocytes with dysfunctional telomeres maintained their mitochondrial respiratory capacity. The upregulation of genes related to mitochondria was observed during senescence in fibroblasts and hepatocytes. These findings suggest that mitochondrial function and activity are preserved in telomere dysfunction-induced senescence.
Article
Biochemistry & Molecular Biology
Hugo Martin, Marie Doumic, Maria Teresa Teixeira, Zhou Xu
Summary: This study provides a mathematical description of the generation-dependent processes underlying non-terminal arrests in telomerase-negative cells. The research highlights differences in the mechanisms and states between non-terminal and terminal senescence arrests. The findings suggest that these two processes contribute to different consequences of senescence at the population level.
CELL AND BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sandor Pentek, Akos Varnagy, Balint Farkas, Peter Mauchart, Krisztina Godony, Timea Varjas, Tamas Koszegi, Peter Kaltenecker, Rita Jakabfi-Csepregi, Kalman Kovacs, Jozsef Bodis, Endre Sulyok
Summary: This study aimed to investigate the relationship between telomere length, telomerase activity, and oxidative DNA damage in IVF patients. The study found that telomere lengths were similar in granulosa cells and follicular fluid, but telomerase activity was significantly reduced and 8-OHdG levels were significantly elevated in follicular fluid. Additionally, high levels of 8-OHdG were associated with negative outcomes in IVF success and clinical pregnancies.
Review
Biochemistry & Molecular Biology
Bibo Li
Summary: Telomere maintenance is crucial for genome stability and regulation of antigenic variation in microbial pathogens. Trypanosomes, such as T. brucei, use a unique DNA polymerase, PolIE, to coordinate the synthesis of telomere G- and C-strands, in contrast to mammalian cells that rely on telomere proteins. Understanding these mechanisms can aid in the development of treatments for diseases caused by these pathogens.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Eric Nguyen, Andrea Richerolle, Julia Sanchez-Bellver, Jacqueline Varennes, Evelyne Segal-Bendirdjian
Summary: This study investigates the effects of ATRA on a subset of breast cancer cell lines and finds that ATRA treatment can inhibit the expression of hTERT, with an inverse relationship to the methylation level of a specific CpG at a region of the hTERT gene. This methylation profile could potentially serve as a biomarker to predict the sensitivity of specific breast cancer subtypes to ATRA-induced hTERT repression.
Review
Biochemistry & Molecular Biology
Semih Can Akincilar, Claire Hian Tzer Chan, Qin Feng Ng, Kerem Fidan, Vinay Tergaonkar
Summary: Telomerase activation is a common feature in cancer, but it does not directly correlate with telomere length. Aberrant expression of shelterin proteins and their release from shortened telomeres can further promote cancer through non-canonical mechanisms.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)