Article
Chemistry, Multidisciplinary
Souvik Sarkar, Raju Laishram, Darshana Deb, Subi J. George
Summary: Dynamic supramolecular polymers, with their functional similarities to classical covalent polymers and their adaptive and self-repairing nature reminiscent of biological assemblies, have emerged as highly promising systems for the design of smart soft materials. Recent advancements in mechanistic investigations and novel synthetic strategies have enhanced our ability to control the primary structure of these supramolecular polymers. In this paper, the authors introduce a remarkable advancement in the synthesis of secondary supramolecular polymers, unveiling new topologies and enhanced higher-order structural complexity.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Tianjian Yang, Kyle Benson, Hailin Fu, Tianrui Xue, Ziyuan Song, Hanyi Duan, Hongwei Xia, Ankarao Kalluri, Jie He, Jianjun Cheng, Challa Kumar, Yao Lin
Summary: This study demonstrates the accelerated assembly of synthetic poly(L-glutamic acid) into amyloid-like fibrils catalyzed by cationic silica nanoparticle clusters as artificial nucleation factors. The charge-charge interactions and surface functionalities effectively facilitate the conformational change of macromolecular monomers and control the rates of nucleation for fibril growth. This approach inspires the development of novel materials through biomimetic supramolecular polymerizations.
Review
Biochemistry & Molecular Biology
Maurizio Brunori
Summary: The process of protein folding is a spontaneous reversible process leading from an ensemble of disordered structures to the ordered functional protein. In addition to the native and denatured states, proteins can also populate a third state called amyloid, which is characteristic of incurable neurodegenerative disorders.
Article
Engineering, Chemical
Yue Liang, Wenjuan Wang, Yan Sun, Xiaoyan Dong
Summary: Inhibition of protein misfolding and aggregation is a major challenge in biochemical and biopharmaceutical engineering. This study investigated the coaggregation and seeding interactions between AB40 and AB11-40, as well as the effect of the small molecule inhibitor EGCG on amyloid aggregation. The results showed that AB11-40 participates in the aggregation of AB40 and that EGCG inhibits both AB isoforms mainly through hydrophobic interactions. These findings provide new insights into AB aggregation and have implications for the development of inhibitors against amyloid protein co-assembly.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Ivana Sirangelo, Clara Iannuzzi
Summary: The study provides an overview of the molecular effects induced by glycation on the amyloid aggregation process of protein models associated with misfolding diseases. Glycation plays a key role in protein folding, kinetics of amyloid formation, and amyloid cytotoxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Nabeela Majid, Rizwan Hasan Khan
Summary: Proteins are vital for cellular functions, but variations in environmental conditions can disrupt their stability and lead to aggregation. Under normal conditions, aggregated proteins are removed by a quality control system, but diseased conditions or impaired systems can result in toxicity. Misfolding and aggregation of certain proteins are linked to diseases like Alzheimer's and Parkinson's. Current treatments are symptomatic and lack the ability to target the cause of these diseases. Therefore, more research is needed to develop drugs that address the root cause. This review provides valuable knowledge and strategies for researchers in neuroscience.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Cell Biology
Alessandra Bigi, Roberta Cascella, Cristina Cecchi
Summary: The misfolding and aggregation of a-synuclein is the key feature of synucleinopathies, such as Parkinson's disease and dementia with Lewy bodies. Soluble oligomeric assemblies formed during the early stages of aggregation are toxic to neurons, while fibrillar conformers contribute to the spreading of the pathology. Recent studies have also shown that a-synuclein fibrils release soluble and highly toxic oligomeric species, leading to immediate dysfunction in recipient neurons. This review discusses the mechanisms of cellular dysfunction caused by a-synuclein oligomers and fibrils in synucleinopathies.
NEURAL REGENERATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Francesca Malagrino, Giuliana Fusco, Valeria Pennacchietti, Angelo Toto, Caterina Nardella, Livia Pagano, Alfonso de Simone, Stefano Gianni
Summary: PDZ domains are common protein-protein interaction modules in the human proteome, often present in multiple repeats. PDZD2 is a protein with six PDZ domains, which undergoes proteolytic cleavage to produce sPDZD2, consisting of a tandem of two PDZ domains, namely PDZ5 and PDZ6. The folding pathway of sPDZD2 involves a transient folding intermediate that may be formed from the denaturation of both PDZ5 and PDZ6 domains. Importantly, this intermediate shows increased affinity for the physiological ligand of sPDZD2 compared to the native state. These findings provide an interesting example of a functionally competent misfolded intermediate.
Review
Chemistry, Physical
Jorge Herrera-Ordonez
Summary: This article provides a personal vision and updated critical review on the controversial aspects of conventional emulsion polymerization (EP). It discusses the mechanisms and kinetics of EP and highlights the challenges and improvements needed in understanding particle formation, colloidal stability, and particle size distribution. The review also emphasizes the alternatives to the traditional mathematical description of diffusion-controlled processes.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Priyanka Borah, Airy Sanjeev, Venkata Satish Kumar Mattaparthi
Summary: Parkinson's disease is a common progressive neurodegenerative disorder caused by misfolding and aggregation of alpha-synuclein. Recent research shows that Oleuropein aglycone (OleA) stabilizes the monomeric structure of alpha-synuclein, preventing the formation of toxic aggregates and favoring the growth of stable ones, highlighting a potential therapeutic approach.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Multidisciplinary Sciences
Kimberly Jia Yi Low, Anandalakshmi Venkatraman, Jodhbir S. Mehta, Konstantin Pervushin
Summary: Protein aggregation and deposition in the form of amyloid fibrils is a characteristic feature of amyloid diseases. While most research focuses on inhibiting amyloid formation, it is equally important to understand the disaggregation mechanisms and their potential therapeutic applications. This review compiles the known mechanisms of amyloid disaggregation and explores the use of disaggregases for treating amyloidosis.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Gunasekhar Burra, Mahmoud B. Maina, Louise C. Serpell, Ashwani K. Thakur
Summary: This study used a reproducible solubilisation protocol to generate a homogenous monomeric solution of the yeast prion protein Sup35 N-terminal hepta-peptide sequence GNNQQNY, revealing molecular details of its self-assembly mechanism. The aggregation kinetics data showed that the GNNQQNY sequence follows nucleation-dependent aggregation kinetics with a critical nucleus of size similar to 7 monomers, where the efficiency of nucleation was inversely related to the reaction temperature.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Shweta Devi, Minal Chaturvedi, Siraj Fatima, Smriti Priya
Summary: Physiological conditions and environmental risk factors play significant roles in influencing protein structure and disease development.
Review
Biochemistry & Molecular Biology
Azadeh Beygmoradi, Ahmad Homaei, Roohullah Hemmati, Pedro Fernandes
Summary: Protein folding is a process that determines the three-dimensional structure of a protein. Recombinant protein production can face problems like proteolysis, incorrect folding, and protein aggregation. Identifying proper protein refolding conditions is challenging, and molecular chaperones can help in proper folding. Proper protein folding is essential for post-translational modifications, and incorrect folding can lead to cellular damage. Understanding recombinant protein expression and addressing folding issues are important.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Soumik Ray, Thomas O. Mason, Lars Boyens-Thiele, Azad Farzadfard, Jacob Aunstrup Larsen, Rasmus K. Norrild, Nadin Jahnke, Alexander K. Buell
Summary: Using mass photometry, it is shown that the Parkinson's disease-related protein, α-synuclein, can form dynamic nanoscale clusters and promote amyloid fibril formation. The formation of these nanoclusters is instantaneous and occurs at much wider ranges of solution conditions. Mass photometry is demonstrated to be a promising methodology for detecting and quantifying nanoscale precursors of phase separation.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)