Journal
BIOCATALYSIS AND BIOTRANSFORMATION
Volume 28, Issue 5-6, Pages 395-402Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/10242422.2010.538949
Keywords
Aeromonas hydrophila; microbial transglycosylation; whole cell biocatalysts; ribavirin; didanosine
Funding
- Universidad Nacional de Quilmes and Secretaria de Ciencia y Tecnica de la Nacion, Argentina
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Microbial transglycosylation is useful as a green alternative in the preparation of purine nucleosides and analogues, especially for those that display pharmacological activities. In a search for new transglycosylation biocatalysts, two Aeromonas hydrophila strains were selected. The substrate specificity of both micro-organisms was studied and, as a result, several nucleoside analogues have been prepared. Among them, ribavirin, a broad spectrum antiviral, and the well-known anti HIV didanosine, were prepared, in 77 and 62% yield using A. hydrophila CECT 4226 and A. hydrophila CECT 4221, respectively. In order to scale-up the processes, the reaction conditions, product purification and biocatalyst preparation were analyzed and optimized.
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