Journal
BIOCATALYSIS AND BIOTRANSFORMATION
Volume 27, Issue 2, Pages 124-130Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10242420802583366
Keywords
Geranyl acetate; solvent free; kinetics; silica immobilized lipase; transesterification
Funding
- Department of Biotechnology
- Council of Scientific and Industrial Research
Ask authors/readers for more resources
The objective of the present work was to study the kinetics of the solvent-free synthesis of geranyl acetate by a novel lipase (activity 60 U g-1) made by immobilization of lipase from Rhizopus oligosporous NRRL 5905 on to cross-linked silica gel. Transesterification was performed with vinyl acetate as the acyl donor. Vinyl acetate was used in large excess compared to geraniol, which made the reaction pseudo first order with respect to geraniol and the reaction rate followed Michaelis-Menten kinetics for a single substrate. To obtain the highest yield for geranyl acetate, various relevant physical parameters such as shaking speed, reaction time, enzyme concentration, initial water amount and reaction temperature that influence the activity of lipase were investigated. A maximum molar conversion of 67% was achieved after 48 h of reaction at 30C, at an enzyme concentration of 25% w/v of reaction mixture. Substrate conversion remained constant for five successive cycles; thereafter the conversion dropped by only 11%. Using a pseudo first-order kinetic model for geranyl acetate synthesis in the absence of organic solvents, apparent Km and Vmax values were evaluated as 60 mM and 141 mol g-1 h-1, respectively.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available