Article
Biochemistry & Molecular Biology
Alejandro Cenalmor, Elena Pascual, Sergio Gil-Manso, Rafael Correa-Rocha, Jose Ramon Suarez, Isabel Garcia-Alvarez
Summary: Neuroinflammation is a critical factor in the progression of Alzheimer's disease and other neurodegenerative disorders. In this study, a series of isatin derivatives were synthesized and evaluated for their anti-neuroinflammatory potential using lipopolysaccharide activated microglia as a cell model. Compound 10 with N-1 alkylation and compound 20 with chlorination showed the best results in reducing nitric oxide, pro-inflammatory interleukin 6, and tumour necrosis factor alpha release by microglial cells, indicating their potential as lead compounds for developing new neuroprotective agents.
Article
Pharmacology & Pharmacy
Nan Zhang, Ying Bian, Lei Yao
Summary: The essential oils extracted from two gardenia varieties have anti-inflammatory effects on murine alveolar macrophages, inhibiting nitric oxide release and reducing the production of TNF-alpha and PGE2. Components such as linalool and alpha-farnesene in the gardenia flower essential oils have potential as anti-inflammatory agents.
Article
Biochemistry & Molecular Biology
Eslam M. H. Ali, Mohammed S. Abdel-Maksoud, Rasha Mohamed Hassan, Karim I. Mersal, Usama M. Ammar, Choi Se-In, Han He-Soo, Hee-Kwon Kim, Anna Lee, Kyung-Tae Lee, Chang-Hyun Oh
Summary: In this study, drug repurposing strategies were used to reposition a series of inhibitors to target P38α kinase, leading to the identification of compounds with anti-inflammatory activity by effectively inhibiting the production of inflammatory cytokines. The most potent inhibitor, compound 11d, exhibited satisfactory inhibitory activity against the production of PGE2 and NO in LPS-stimulated macrophages.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yue Wang, Ruiwei Ye, Liming Fan, Xin Zhao, Linxue Li, Hao Zheng, Yan Qiu, Xiuxia He, Yiming Lu
Summary: The study demonstrates that an anti-tumor necrosis factor-alpha (TNF-α) peptide, SN1-13, has anti-inflammatory activity by inhibiting TNF-α-induced cytotoxicity and blocking TNF-α-triggered signaling pathways. Molecular docking analysis also suggests that SN1-13 can disrupt the binding between TNF-α and its receptors, TNFR1 and TNFR2. These findings indicate that SN1-13 has potential as a therapeutic peptide for TNF-α-mediated inflammatory diseases.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Analu Rocha Costa, Rodrigo Novais Franca, Izaltina Silva-Jardim, Raner Jose Santana Silva, Jane Lima-Santos, Luiz Carlos Salay, Rodrigo Luis Silva Ribeiro Santos
Summary: This study investigated the colloid phenomena between 3-methyl-5-phenyl-pyrazoline-1-(S-benzyldithiocarbazate) (DTC) and Pluronic F127 through fluorescence measurements, revealing that the DTC-based micellar system is not cytotoxic to Swiss mice peritoneal macrophages and induces nitric oxide (NO) production in these cells.
COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
(2021)
Article
Oncology
Birandra K. Sinha, Erik J. Tokar, Jianying Li, Pierre R. Bushel
Summary: The nitric oxide donor NCX4040 exhibits cytotoxicity towards ovarian tumor cells by generating reactive oxygen species and inducing DNA damage. It also depletes cellular glutathione and induces oxidative stress, resulting in tumor cell death. NCX4040 treatment leads to the induction of oxidative stress genes, inflammatory response genes, DNA damage response genes, and MAP kinase response genes.
Article
Environmental Sciences
Heba O. Mohammed, Eman Ahmed Alaa El-Din, Azza I. Farag
Summary: The study found that the use of electronic cigarettes resulted in marked pathological alterations in the colonic mucosa, which could be attributed to oxidative and inflammatory stresses. However, cessation of exposure led to recovery in the colonic mucosal histological structure, downregulation of inflammatory markers, and restoration of oxidant/antioxidant balance.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Mi-Aie Hwang, Misun Won, Joo-Young Im, Mi-Jung Kang, Dae-Hyuk Kweon, Bo-Kyung Kim
Summary: Our study aimed to investigate the impact of macrophages on the invasive ability of gastric cancer cells and explore its potential mechanism. Through transcriptome analysis, we found that integrin alpha V gene expression increased in AGS cells cocultured with RAW264.7 cells. Additionally, AGS cells cocultured with RAW264.7 cells exhibited increased adhesion and invasion compared to cells cultured alone. This increased invasion was inhibited by knockdown of integrin alpha V.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Shevanuja Theivendran, Zhengying Gu, Jie Tang, Yannan Yang, Hao Song, Yang Yang, Min Zhang, Dan Cheng, Chengzhong Yu
Summary: This study reports the synthesis of a nanoparticle-based NO delivery platform that utilizes tetrasulfide-modified organosilica nanoparticles for effective NO delivery and macrophage activation. The enhanced intracellular delivery of NO resulted in macrophage activation and delayed tumor growth in a breast cancer model.
Article
Biochemistry & Molecular Biology
Amelia Barilli, Giulia Recchia Luciani, Rossana Visigalli, Roberto Sala, Maurizio Soli, Valeria Dall'Asta, Bianca Maria Rotoli
Summary: This study explores the mechanisms underlying the modulation of iNOS expression in human alveolar cells. The researchers found that cytokines secreted by SARS-CoV-2 spike S1-activated macrophages markedly induce iNOS expression, which can be suppressed by the drug baricitinib. These findings are important for understanding the inflammatory response mechanisms in COVID-19 and identifying potential therapeutic drugs.
Article
Biochemical Research Methods
Gazanfar Ahmad, Reyaz Hassan, Neerupma Dhiman, Asif Ali
Summary: This study isolated 3-Acetylmyricadiol, a pentacyclic triterpenoid, from the ethyl acetate bark extract of Myrica esculenta, and evaluated its anti-inflammatory potential. The results showed that 3-Acetylmyricadiol inhibited the production of nitric oxide, IL-6, and TNF-alpha in a dose-dependent manner, suggesting its potential as an effective anti-inflammatory molecule.
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
(2022)
Article
Pharmacology & Pharmacy
Romaissaa Mokdad, Cendrine Seguin, Sylvie Fournel, Benoit Frisch, Beatrice Heurtault, Abdelkader Hadjsadok
Summary: The aim of this study was to formulate liposomes encapsulating highly mineralized thermal waters and investigate their anti-inflammatory effect on stimulated macrophage cells. The results showed that the liposomes loaded with thermal waters had good physical stability and demonstrated anti-inflammatory effects, making them suitable for treating inflammatory skin diseases.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Ahmed K. Aladhami, Christian A. Unger, Shannon L. Ennis, Diego Altomare, Hao Ji, Marion C. Hope, Kandy T. Velazquez, Reilly T. Enos
Summary: Macrophage-derived TNF-alpha does not play a significant role in the development of obesity, but can reduce TNF-alpha gene expression and circulating levels in adipose tissue, as well as modulate specific signaling pathway gene expression.
Article
Medicine, Research & Experimental
Ildernandes Vieira-Alves, Antonielle Rodrigues Pereira Alves, Natalia Muradas Valerio Souza, Tales Leonardo de Melo, Leda Maria de Castro Coimbra Campos, Larisse de Souza Barbosa Lacerda, Celso Martins Queiroz-Junior, Ana Claudia dos Santos Pereira Andrade, Luciola Silva Barcelos, Mauro Martins Teixeira, Vivian Vasconcelos Costa, Steyner F. Cortes, Virginia S. Lemos
Summary: Poor disease outcomes and lethality in beta-coronavirus infections are directly related to endothelial dysfunction. This study investigated the mechanisms underlying vascular dysfunction caused by the betacoronaviruses MHV-3 and SARS-CoV-2. The results showed that MHV-3 infection reduced contractility, blood pressure, and blood flow, leading to death. Similar vascular dysfunction was observed in SARS-CoV-2 infection. These findings highlight the important role of the vascular endothelium and TNF in the pathogenesis and lethality of coronaviruses.
Article
Oncology
Paige C. Arneson-Wissink, Jason D. Doles
Summary: Skeletal muscle wasting is a key driver of negative clinical outcomes and is associated with a range of pathologies including cancer. Cancer cachexia is a multi-factorial syndrome that includes skeletal muscle wasting, but remains understudied. Research suggests that amino acid metabolic disruption is linked to myoblast defects induced by cancer cell conditioned media.
EXPERIMENTAL CELL RESEARCH
(2021)
