Article
Surgery
Siv Fonnes, Tilde Rasmussen, Amanda Brunchmann, Barbara Juliane Holzknecht, Jacob Rosenberg
Summary: This systematic review and meta-analysis aimed to characterize the suspected or confirmed abdominal diseases and surgeries associated with Yersinia infection. The results showed that approximately half of the patients with mesenteric lymphadenitis and terminal ileitis were serologically positive for Yersinia spp. Infections, which may lead to unnecessary surgeries for suspected appendicitis.
JOURNAL OF SURGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jyoti M. Gurung, Ayad A. A. Amer, Shiyun Chen, Andreas Diepold, Matthew S. Francis
Summary: YscX, an important component of the Yersinia type III secretion system, remains poorly understood in terms of its function and mode of action. This study identified an N-terminal secretion domain in YscX that promotes its own secretion and contains essential information for its function. The secretion permissiveness of YscX is closely related to the assembly of an operational secretion system.
MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Laura Blomvall, Kirsti Pelkola, Taru Lienemann, Suvi Lehtoniemi, Leena Pohjola, Maria Fredriksson-Ahomaa
Summary: A Y. pseudotuberculosis outbreak was diagnosed in a male turkey flock in Finland, causing osteomyelitis and lameness. Whole-genome sequencing revealed that all isolates belonged to the same strain, indicating a common source of infection. High rates of carcass condemnation were observed in the slaughterhouse, highlighting the importance of food chain information.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Keke Fei, Hong-Jun Chao, Yangbo Hu, Matthew S. Francis, Shiyun Chen
Summary: The CpxRA two-component regulatory system and Rcs phosphorelay system in Enterobacteriaceae are both involved in maintaining bacterial envelope integrity under stress. This study demonstrates a novel regulatory cascade where CpxR negatively regulates RcsB, impacting the activation of the Ysc-Yop T3SS. Activation of Cpx signaling represses activity of rcsB promoters, while a phosphorylation site mutation in CpxR renders it inactive in regulating output from these promoters.
Article
Plant Sciences
Naama Wagner, Michael Alburquerque, Noa Ecker, Edo Dotan, Ben Zerah, Michelle Mendonca Pena, Neha Potnis, Tal Pupko
Summary: In this study, novel machine-learning algorithms were developed to characterize the secretion signal of Type III effectors in both plant and animal pathogens. A benchmark dataset was curated and the results showed that the novel approach had substantially better classification accuracy compared to previous methodologies. The study also revealed that plant and animal pathogen effectors may have different secretion signals.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Bailey Milne-Davies, Stephan Wimmi, Andreas Diepold
Summary: The type III secretion system is the common core of two bacterial molecular machines: the flagellum and the injectisome. It is characterized by flexibility, adaptivity, and a dynamic nature.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Hanh N. Lam, Tannia Lau, Adam Lentz, Jessica Sherry, Alejandro Cabrera-Cortez, Karen Hug, Annalyse Lalljie, Joanne Engel, R. Scott Lokey, Victoria Auerbuch
Summary: The development of new antibiotics is urgently needed due to the emerging threat of antibiotic-resistant bacteria. A newly discovered isomer, 4EpDN, shows potent inhibitory effects on multiple Gram-negative bacteria's injectisome type III secretion system (T3SS), possibly by preventing complete T3SS assembly.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Review
Biochemistry & Molecular Biology
Amit Meir, Kevin Mace, Yogesh Vegunta, Sunanda M. Williams, Gabriel Waksman
Summary: This review provides an update on recent advances in substrate recruitment and delivery by recruitment platforms associated with Type III, IV, and VI secretion systems in gram-negative bacteria.
TRENDS IN MICROBIOLOGY
(2023)
Review
Microbiology
Elizabeth A. Rucks
Summary: Type III secretion systems (T3SS) are utilized by Gram-negative pathogens to deliver effector proteins into target eukaryotic cells for manipulating cell functions and enhancing pathogenesis. This review will discuss the history, biochemical characterization, and function of chlamydial T3SS, as well as the use of heterologous/surrogate models for studying it. The review will also cover the history of chlamydial effectors and recent advances in the field.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Biao Yuan, Athina G. Portaliou, Rinky Parakra, Jochem H. Smit, Jiri Wald, Yichen Li, Bindu Srinivasu, Maria S. Loos, Harveer Singh Dhupar, Dirk Fahrenkamp, Charalampos G. Kalodimos, Franck Duong van Hoa, Thorben Cordes, Spyridoula Karamanou, Thomas C. Marlovits, Anastassios Economou
Summary: Type III protein secretion is common in Gram-negative pathogens, with SctV forming peripheral oligomeric clusters in EPEC and serving as a key receptor for different chaperone/exported protein pairs. The dynamic motions of SctV protomers, modulated by chaperones and ATPase, may allosterically affect the secretion process.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yong-Liang Gan, Li-Yan Yang, Li-Chao Yang, Wan-Lian Li, Xue-Lian Liang, Wei Jiang, Guo-Feng Jiang, Xiao-Hong Hang, Mei Yang, Ji-Liang Tang, Bo-Le Jiang
Summary: The study identified a novel domain at the C-terminal end of HpaB in Xanthomonas campestris pv. campestris that contributes to virulence and hypersensitive response, revealing its involvement in the disassembly of chaperone-effector complex during the secretion of T3Es.
Article
Microbiology
Sukriti Prashar, Miguel Portales Guemes, Poorandai Shivbaran, Eugenia Jimenez Alvarez, Christopher Soha, Samir Nacer, Michael McDonough, Gregory Plano, Julie Torruellas Garcia
Summary: The research aimed to develop a novel disk diffusion assay to evaluate the susceptibility of Yersinia pestis to type III secretion system inhibitors using magnesium oxalate agar; the assay successfully detected inhibition of Y. pestis T3SS and may be useful for screening small molecules targeting bacterial T3SS or testing susceptibility to T3SS-targeting drugs.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Marta Nieckarz, Karolina Jaworska, Adrianna Raczkowska, Katarzyna Brzostek
Summary: This study investigates the role of the transcriptional regulator OmpR in membrane protein expression and secretion in Yersinia enterocolitica. The findings show that OmpR negatively affects the levels of Ysc-Yop proteins, particularly YopD, and represses the expression of yopD. OmpR also has a positive role in the expression of the master regulator of the flagellar-associated T3SS, flhDC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Tao Liu, Zheng-Yang Zhou, Chen-wang Lu, Shang-Jie Bai, Jun Wang, Gao-Xue Wang
Summary: In this study, the researchers found that the T4SS of Yersinia ruckeri SC09 mediated bacterial virulence and immune evasion, possibly through the secretion of molecules such as STIR-1, STIR-2, and STIR-3. These findings contribute to a better understanding of the general rules of bacterial pathogenicity.
Article
Biochemistry & Molecular Biology
Oanh Ho, Per Rogne, Tomas Edgren, Hans Wolf-Watz, Frederic H. Login, Magnus Wolf-Watz
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Cell Biology
Helene H. Jensen, Mikkel R. Holst, Frederic H. Login, Jeanette J. Morgen, Lene N. Nejsum
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Frederic H. Login, Helene H. Jensen, Gitte A. Pedersen, Manuel R. Amieva, Lene N. Nejsum
Article
Biochemistry & Molecular Biology
Frederic H. Login, Helene H. Jensen, Gitte A. Pedersen, Jennifer S. Koffman, Tae-Hwan Kwon, Maddy Parsons, Lene N. Nejsum
Review
Cell Biology
Sarannya Edamana, Frederic H. Login, Soichiro Yamada, Tae-Hwan Kwon, Lene N. Nejsum
Summary: Aquaporins (AQPs) are proteins that facilitate passive transport of water across cellular membranes, playing important roles in physiology such as regulation of body water homeostasis, skin hydration, and fat metabolism. Dysregulation of AQPs is associated with various pathophysiological conditions and major players in multiple cancers, influencing cellular signaling, migration, proliferation, and regulation of junctional proteins involved in cell-cell adhesion.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Cell Biology
H. Login, Johan Palmfeldt, Joleen S. Cheah, Soichiro Yamada, Lene N. Nejsum
Summary: The overexpression of AQP5 in cancers may contribute to carcinogenesis and cancer spread through two independent mechanisms: reduced cell-cell adhesion and increased cell migration capacity.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Cell Biology
Mikkel R. Holst, Louis G. Jensen, Jesse Aaron, Frederic H. Login, Sampavi Rajkumar, Ute Hahn, Lene N. Nejsum
Summary: Regulated vesicle exocytosis, in response to extracellular stimuli, is crucial for processes like short-term urine concentration. In renal collecting ducts, AQP2-containing vesicles fuse with the plasma membrane upon stimulation by arginine vasopressin, enhancing water reabsorption. The 3D organization of these vesicles, which are similar in size to synaptic vesicles and associate with F-actin, was analyzed using 3D superresolution microscopy in a cell culture system.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Physiology
Christina Ernstsen, Frederic H. Login, Anne-Sofie B. Schelde, Jacob R. Therkildsen, Jakob Moller-Jensen, Rikke Norregaard, Helle Praetorius, Lene N. Nejsum
Summary: This study found that the urinary concentration defect in patients with acute pyelonephritis may be related to abnormal cellular localization of AQP2. Pathogenic bacteria can induce membrane targeting of AQP2, and this effect does not depend on an increase in cAMP levels or phosphorylation of the S256 site.
Review
Immunology
Anne Sofie Bruun-Sorensen, Sarannya Edamana, Frederic H. Login, Signe Borgquist, Lene N. Nejsum
Summary: Aquaporins, proteins that facilitate passive water transport across cellular membranes, are often overexpressed in various cancers, including pancreatic ductal adenocarcinoma. This overexpression of aquaporins, such as aquaporin-1, aquaporin-3, and aquaporin-5, has been linked to metastasis and poor survival in pancreatic cancer, suggesting their potential as diagnostic markers and therapeutic targets.
Review
Immunology
Malte Bystrup, Frederic H. Login, Sarannya Edamana, Signe Borgquist, Trine Tramm, Tae-Hwan Kwon, Lene N. Nejsum
Summary: Aquaporin-5 (AQP5) is essential in transepithelial water transport and its ectopic overexpression in breast cancer is associated with lymph node metastasis and poor prognosis. In addition to its role in water transport, AQP5 also plays a role in cancer metastasis, migration, and proliferation. Understanding the molecular mechanisms of AQP5 in cancer development and progression is crucial for its potential use as a prognostic biomarker and for the development of targeted interventions for breast cancer treatment.
Review
Immunology
Laura Traberg-Nyborg, Frederic H. Login, Sarannya Edamana, Trine Tramm, Signe Borgquist, Lene N. Nejsum
Summary: The overexpression of aquaporin in breast cancer is associated with poor prognosis and affects cellular processes like migration, angiogenesis, and proliferation, providing potential mechanisms for the progression and metastasis of breast cancer.
Article
Cell Biology
Sarannya Edamana, Frederic H. Login, Andreas Riishede, Vibeke S. Dam, Trine Tramm, Lene N. Nejsum
Summary: Breast carcinomas are associated with increased cell proliferation, migration, altered cellular adhesion, and loss of epithelial polarity. Aquaporin-5 (AQP5) is aberrantly expressed in breast cancer and is linked to metastasis and poor prognosis. AQP5 enhances cancer cell proliferation and migration, reduces cell-cell adhesion, and activates the Ras pathway. This study explores the relationship between AQP5 and the polarity protein Scribble. Results show that high AQP5 expression is inversely related to Scribble levels in breast carcinoma tissue samples. Overexpression of AQP5 in breast cancer cells reduces spheroid size, circularity, and Scribble levels. The effect of AQP5 on Scribble is partially dependent on AQP5-mediated activation of Ras. These findings suggest that AQP5 negatively regulates cellular polarity in breast cancer.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell Biology
Stine Julie Tingskov, Mariagrazia D'Agostino, Frederic H. Login, Grazia Tamma, Lene N. Nejsum, Rikke Norregaard
Summary: In this study, the effect of tamoxifen (TAM) on the expression and localization of aquaporin 3 (AQP3) in collecting ducts was investigated. TAM attenuated the downregulation of AQP3 in a unilateral ureteral obstruction (UUO) model and a lithium-induced nephrogenic diabetes insipidus (NDI) model, and affected the intracellular localization in the collecting ducts. TAM also influenced the expression profile of other basolateral proteins.
Review
Urology & Nephrology
Frederic H. Login, Lene N. Nejsum
Summary: Aquaporins play a crucial role in renal water handling and body water homeostasis. They are also involved in water reabsorption and secretion in other tissues, transport of small molecules and gases, and various cellular processes. Furthermore, the expression of certain AQPs is related to cancer progression and drug response, suggesting their potential application in prognostics and therapeutics.
NATURE REVIEWS NEPHROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Helene H. Jensen, Frederic H. Login, Ji-Young Park, Tae-Hwan Kwon, Lene N. Nejsum
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)