Article
Microbiology
Giang Ngo, Melis Girbas, Hannah Schaetzle, Andreas Hammer, Schara Safarian, Maximilian Huebinger, Enrico Schleiff
Summary: This study investigated the function and genomic characteristics of two outer membrane transporters, TpsB1 and TpsB2, in Anabaena sp. strain PCC 7120, revealing their roles in substrate transport and membrane integrity in cyanobacteria. Experimental and bioinformatics analyses suggested a crucial function of these proteins in cyanobacterial physiology.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Multidisciplinary Sciences
Clifford Manyo Ntui, James M. Fleckenstein, Wolf-Dieter Schubert
Summary: Enterotoxigenic Escherichia coli (ETEC) is a diarrhoeal pathogen without a vaccine, but a potential vaccine antigen called EtpA has been identified. EtpA is a conserved secreted adhesin that binds to flagella tips to attach ETEC to the host intestine. The folding structure of EtpA provides a template for extending the protein into the C-terminal domains.
Article
Microbiology
Stephen C. Watts, Louise M. Judd, Rosemary Carzino, Sarath Ranganathan, Kathryn E. Holt
Summary: Respiratory infection during childhood is a key risk factor in early cystic fibrosis lung disease progression. Haemophilus influenzae and Haemophilus parainfluenzae are commonly isolated from the lungs of children with CF, with a high frequency of colonization and antimicrobial resistance. The genetic basis for AMR in these bacteria was identified, and Haemophilus parainfluenzae had a higher prevalence of acquired AMR genes than H. influenzae.
Article
Biochemistry & Molecular Biology
Marufa Nasreen, Remya Purushothaman Nair, Alastair G. McEwan, Ulrike Kappler
Summary: Sulfoxide-damage repair mechanisms are crucial for the virulence of bacterial pathogens. The periplasmic protein MsrAB in the human respiratory pathogen Haemophilus influenzae plays a key role in resisting reactive chlorine species. It also modulates the host immune response. Our study reveals the enzymatic properties of MsrAB, its catalytic activity, and potential substrates. This research sheds light on the maintenance of adhesins and essential metabolic processes in H. influenzae.
Article
Biochemistry & Molecular Biology
Yesenia Osorio-Aguilar, Maria Cristina Gonzalez-Vazquez, Patricia Lozano-Zarain, Ygnacio Martinez-Laguna, Lidia Baylon-Pacheco, Jose Luis Rosales-Encina, Alejandro Carabarin-Lima, Rosa del Carmen Rocha-Gracia
Summary: Enolase proteins can interact with extracellular matrix proteins, such as collagens, to promote the colonization and invasion of pathogens. The study shows that non-typeable H. influenzae enolase strongly binds to type I collagen and has a higher affinity for it than type III collagen. This interaction may enhance the virulence and pathogenicity of H. influenzae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Joel Manyahi, Sabrina J. J. Moyo, Nina Langeland, Bjorn Blomberg
Summary: This study used next generation sequencing to determine the mechanisms of antibiotic resistance among penicillin non-susceptible Streptococcus pneumoniae (PNSP) isolates. The results showed that common resistance genes in PNSP include erm(B) and mef(A)-msr(D) for macrolide resistance, and tet(M) for tetracycline resistance. These resistance genes are associated with the Tn6009 transposon.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Microbiology
Caelin C. Potts, Lorraine D. Rodriguez-Rivera, Adam C. Retchless, Sean A. Buono, Alexander T. Chen, Henju Marjuki, Amy E. Blain, Xin Wang
Summary: Antibiotics play a crucial role in the treatment and prevention of invasive Haemophilus influenzae disease. An antimicrobial resistance survey conducted in 2016 on invasive H. influenzae isolates collected in the United States showed that the majority of the isolates remained susceptible to clinically relevant antibiotics, except for ampicillin.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
A. D. Cox, R. Kuo Lee, M. Ulanova, M. G. Bruce, R. S. W. Tsang
Summary: The workshop organized by PHAC, CIHR, and NRC brought together experts from PAHO and its regional labs in South America, USA, and Canada to discuss the current epidemiology of invasive Haemophilus influenzae disease in the Americas. The event built upon previous recommendations and included updated data.
Article
Public, Environmental & Occupational Health
Shrijana Shrestha, Meeru Gurung, Puja Amatya, Sanjeev Bijukchhe, Anindya Sekhar Bose, Michael J. Carter, Madhav C. Gautam, Sunaina Gurung, Jason Hinds, Rama Kandasamy, Sarah Kelly, Bibek Khadka, Pratistha Maskey, Yama F. Mujadidi, Peter J. O'Reilly, Bhishma Pokhrel, Rahul Pradhan, Ganesh P. Shah, Sonu Shrestha, Brian Wahl, Katherine L. O'Brien, Maria Deloria Knoll, David R. Murdoch, Dominic F. Kelly, Stephen Thorson, Merryn Voysey, Andrew J. Pollard
Summary: This study investigated the impact of introducing PCV10 vaccine on pneumococcal carriage and disease in children in Nepal. The results showed a reduction in vaccine serotype carriage in both healthy children and children under 2 years with pneumonia. However, there was an increase in carriage of serotypes 19A and 3, emphasizing the importance of continued surveillance.
LANCET GLOBAL HEALTH
(2022)
Article
Multidisciplinary Sciences
James S. Davies, Michael J. Currie, Rachel A. North, Mariafrancesca Scalise, Joshua D. Wright, Jack M. Copping, Daniela M. Remus, Ashutosh Gulati, Dustin R. Morado, Sam A. Jamieson, Michael C. Newton-Vesty, Gayan S. Abeysekera, Subramanian Ramaswamy, Rosmarie Friemann, Soichi Wakatsuki, Jane R. Allison, Cesare Indiveri, David Drew, Peter D. Mace, Renwick C. J. Dobson
Summary: The researchers report the structure of the TRAP transporter and propose a model for substrate transport using an 'elevator-with-an-operator' mechanism.
NATURE COMMUNICATIONS
(2023)
Article
Infectious Diseases
Anneke Steens, Kamelia R. Stanoeva, Mirjam J. Knol, Rob Mariman, Hester E. de Melker, Nina M. van Sorge
Summary: While the incidence of most respiratory-transmitted diseases decreased during the COVID-19 pandemic, there was an increase in invasive disease caused by Haemophilus influenzae b (Hib) in the Netherlands, affecting both vaccinated and unvaccinated age groups. The vaccine effectiveness against Hib invasive disease remained unchanged, with a high effectiveness rate exceeding 90%. Various factors contributing to this rise in invasive disease were discussed.
Article
Infectious Diseases
Abdollah Karimi, Sedigheh Rafiei Tabatabaei, Leila Azimi, Nasim Almasian Tehrani, Fatemeh Fallah, Iman Faghihian
Summary: The death rate from meningitis remains high in certain parts of the world. The specific cause of meningitis needs to be identified in order to determine the appropriate treatment. This study investigated the false-negative results of multiplex RT-PCR for detecting Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis serogroup using two different molecular methods.
CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Julian Trouillon, Ina Attree, Sylvie Elsen
Summary: Two-partner secretion (TPS) systems, also known as Type Vb secretion systems, are important for the translocation of effector proteins in Gram-negative bacteria. They play a crucial role in bacterial pathogenesis and host interactions by secreting different classes of effectors. This review highlights the regulation of TPS systems and the common regulatory mechanisms across different bacterial species. Understanding the context-dependent regulation of TPS systems is essential, as common regulatory cues during infection affect TPS systems across distant species, representing conserved global infection-related regulatory mechanisms.
MOLECULAR MICROBIOLOGY
(2023)
Review
Pediatrics
Lin Yang, YaFei Zhu, GuangSheng Wu
Summary: The purpose of this study is to improve the clinical understanding of acute empyema caused by Haemophilus influenzae by analyzing the clinical data of a child with this condition and conducting a literature review. The study found that comprehensive treatment, including antibiotics, closed pleural drainage, and intrapleural injection of urokinase, effectively controlled the patient's condition.
FRONTIERS IN PEDIATRICS
(2022)
Article
Immunology
Julia W. Angkeow, Daniel R. Monaco, Athena Chen, Thiagarajan Venkataraman, Sahana Jayaraman, Cristian Valencia, Brandon M. Sie, Thomas Liechti, Payam N. Farhadi, Gabriela Funez-dePagnier, Cheryl A. Sherman-Baust, May Q. Wong, Ingo Ruczinski, Patrizio Caturegli, Cynthia L. Sears, Patricia J. Simner, June L. Round, Priya Duggal, Uri Laserson, Theodore S. Steiner, Ranjan Sen, Thomas E. Lloyd, Mario Roederer, Andrew L. Mammen, Randy S. Longman, Lisa G. Rider, H. Benjamin Larman
Summary: Microbial exposures play a crucial role in impacting healthspan through shaping the immune system and microbiota. The use of Phage ImmunoPrecipitation Sequencing (PhIP-Seq) allows for high-throughput and cost-effective detection of exposure and response to microbial protein products. This study demonstrates that PhIP-Seq with the ToxScan library is an effective tool for studying the environmental determinants of health and disease at a cohort scale.