Article
Biochemistry & Molecular Biology
Rossella Cannarella, Francesca Mancuso, Nunziata Barone, Iva Arato, Cinzia Lilli, Catia Bellucci, Marco Musmeci, Giovanni Luca, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: This study evaluated the expression of the follicle-stimulating hormone receptor (FSHR) in human spermatozoa and its effects on sperm function. The results showed that FSHR is expressed in the post-acrosomal segment, neck, midpiece, and tail of human spermatozoa, with no difference in localization between patients and controls. Incubation with FSH increased FSHR expression in patients. Furthermore, FSH incubation had a negative effect on sperm motility and mitochondrial function in both patients and controls, but more severely in controls. FSH also activated the AKT(473) signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Marwa Lahimer, Debbie Montjean, Rosalie Cabry, Severine Capelle, Elodie Lefranc, Veronique Bach, Mounir Ajina, Habib Ben Ali, Hafida Khorsi-Cauet, Moncef Benkhalifa
Summary: It has been found that advanced age negatively affects sperm parameters and DNA integrity, with potential impacts on in vitro fertilization success. This study aimed to understand the association between advanced paternal age and sperm parameters, DNA integrity, and DNA methylation profile. The findings showed that individuals over 40 years old had significantly higher levels of DNA fragmentation (15% versus 9%, p = 0.04) and lower global methylation levels (6% in the young group versus 2% in the old group, p = 0.03). These results emphasize the importance of assessing sperm DNA fragmentation, particularly after the age of 40, in fertility evaluation and treatment.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Costanza Cimini, Marina Ramal-Sanchez, Angela Taraschi, Flavio Della Pelle, Annalisa Scroccarello, Ramses Belda-Perez, Luca Valbonetti, Paola Lanuti, Marco Marchisio, Mario D'Atri, Claudio Ortolani, Stefano Papa, Giulia Capacchietti, Nicola Bernabo, Dario Compagnone, Barbara Barboni
Summary: Nowadays, there is a growing adoption of In Vitro Fertilization (IVF) techniques. A promising strategy is the use of non-physiological materials and naturally derived compounds for advanced sperm preparation methods. The study showed that MoS2/Catechin nanoflakes and catechin (CT) did not have any negative effects on sperm capacitation, while adding a specific concentration of CT increased the fertilizing ability of sperm in an IVF assay. These findings suggest that catechins and new materials obtained using natural or bio compounds could enhance current sperm capacitation strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Fisheries
Olena Shaliutina, Anna Materiienko, Anna Shaliutina-Kolesova, Ievgeniia Gazo
Summary: In recent years, there has been a push to develop new in vitro techniques for ecotoxicology and environmental risk assessment, with fish spermatozoa being considered a promising tool due to their simplicity and availability. However, further studies are needed to establish a clear correlation between in vitro and in vivo toxicities when using fish spermatozoa.
Review
Endocrinology & Metabolism
Peter T. K. Chan, Bernard Robaire
Summary: This review discusses the impact of advanced paternal age on offspring and explores the relationships between advanced paternal age and fertility, perinatal health, cancer risk, genetic diseases, and neurodevelopmental effects. The potential mechanisms underlying the effects of advanced paternal age on germ cells and the current state of reproductive risk management are also presented. The challenges and approaches to address this important clinical dilemma are outlined.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Paula Saez-Espinosa, Laura Robles-Gomez, Leonor Ortega-Lopez, Jon Aizpurua, Maria Jose Gomez-Torres
Summary: This study provides new insights into globozoospermia by evaluating different structural, functional and molecular sperm biomarkers. The findings could have potential implications in improving sperm selection methods for assisted reproductive techniques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Min Zhang, Riccardo Zenezini Chiozzi, David A. Skerrett-Byrne, Tineke Veenendaal, Judith Klumperman, Albert J. R. Heck, Brett Nixon, J. Bernd Helms, Bart M. Gadella, Elizabeth G. Bromfield
Summary: This study provides in-depth characterization of the perinuclear theca (PT) of boar spermatozoa through subcellular fractionation, revealing 1802 identified proteins. The PT was found to be enriched in proteins involved in sperm-egg fusion and egg activation, as well as endoplasmic reticulum (ER) proteins and the ER-stress response. Protein-protein interaction analysis visualized an intricate network of PT protein complexes, including proteasome subunits.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Fisheries
S. Judycka, D. Zarski, M. A. Dietrich, H. Karol, P. Hliwa, M. Blazejewski, A. Ciereszko
Summary: The study focuses on an improved semen cryopreservation protocol for commercial fertilization in European perch, showing high sperm motility with a new extender and KCl supplementation. The enhanced procedure allows for handling prediluted semen and maintaining high fertilization rates for cryopreserved and fresh semen, enabling large-scale fertilization applications.
Article
Behavioral Sciences
Callum S. McDiarmid, Roger Li, Ariel F. Kahrl, Melissah Rowe, Simon C. Griffith
Summary: Research on sperm is important in various fields such as ecology and evolution. Sperm Sizer, a freely available Java program, can semi-automate the process of measuring sperm length efficiently. The program produces high quality sperm length data and can be used in various species.
BEHAVIORAL ECOLOGY AND SOCIOBIOLOGY
(2021)
Review
Developmental Biology
Nicoletta Tarozzi, Marco Nadalini, Giovanni Coticchio, Carlotta Zaca, Cristina Lagalla, Andrea Borini
Summary: Sperm is crucial for reconstitution of embryonic diploidy and specialized developmental functions. It triggers activation through PLC-zeta after gamete fusion, impacting fertilization and chromosome segregation. Sperm-specific epigenetic factors also contribute to embryonic development and have implications for assisted reproduction treatments.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Nathalie Le Foll, Jean-Christophe Pont, Audrey L'Hostis, Thomas Guilbert, Frederic Bouillaud, Jean-Philippe Wolf, Ahmed Ziyyat
Summary: cFEE improves gamete interaction in humans by enhancing sperm movement parameters and increasing the percentage of hyperactivated sperm. However, it does not significantly impact the analyzed mitochondrial bioenergetic parameters, indicating that it may act through a more efficient use of energy resources.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Developmental Biology
Nicoletta Tarozzi, Marco Nadalini, Giovanni Coticchio, Carlotta Zaca, Cristina Lagalla, Andrea Borini
Summary: Sperm is essential for embryonic development, playing roles in activation, development, chromosome segregation, and more. Sperm's contribution to development goes beyond just fertilization, with implications for embryo health and assisted reproduction treatments.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Victoria Nikitaras, Deirdre Zander-Fox, Nicole O. McPherson
Summary: Research shows that in vitro, the addition of idebenone can reduce ROS concentrations in sperm from older fathers, leading to improved fertilization rates, embryo quality, and implantation rates.
Article
Andrology
Georges Raad, Fadi Fakih, Marwa Bazzi, Vinal Massaad, Estrellita Nasrallah, Zalihe Yarkiner, Youmna Mourad, Danielle Abou Khater, Rita Balech, Christine Saliba, Munevver Serdarogullari, Chadi Fakih
Summary: This study aimed to investigate the effect of Lactobacillus plantarum secretions on sperm motility, morphology, and DNA fragmentation when added to the sperm freezing medium. The results showed that the addition of 10^8 colony-forming units/mL Lactobacillus plantarum secretions did not significantly decrease sperm motility after thawing. Furthermore, the Lactobacillus plantarum secretions were found to protect sperm DNA integrity more effectively in the non-normozoospermia group.
Article
Biochemistry & Molecular Biology
Giulia Traini, Lara Tamburrino, Maria Emanuela Ragosta, Giulia Guarnieri, Annamaria Morelli, Linda Vignozzi, Elisabetta Baldi, Sara Marchiani
Summary: Benzo(a)pyrene (BaP) is a dangerous air pollutant that can have adverse effects on human spermatozoa, including reproductive toxicity. In vitro experiments showed that BaP reduced sperm motility and migration, increased spontaneous acrosome reaction and DNA fragmentation, but had no effects on apoptotic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Fabiana B. Kohlrausch, David L. Keefe
Review
Toxicology
Kaio Cezar Rodrigues Salum, Marcos Cesar Santos Castro, Angela Santos Ferreira Nani, Fabiana Barzotto Kohlrausch
INHALATION TOXICOLOGY
(2020)
Review
Obstetrics & Gynecology
Fabiana B. Kohlrausch, Thalita S. Berteli, Fang Wang, Paula A. Navarro, David L. Keefe
Summary: Maintenance of genome integrity is crucial for mammalian development in germline and preimplantation embryos. L1 retrotransposon plays an important role in germline and early embryo development in mice and humans, and its activation and expression are essential for preimplantation development. Although there have been studies on L1 expression at different stages of development, the developmental consequences of this expression remain poorly understood, and further research is needed to elucidate the relationship between L1 retrotransposition events and genomic instability during germline and early embryo development.
REPRODUCTIVE SCIENCES
(2022)
Article
Genetics & Heredity
Kaio Cezar Rodrigues Salum, Guilherme Orofino de Souza, Gabriella de Medeiros Abreu, Mario Campos Junior, Fabiana Barzotto Kohlrausch, Joao Regis Ivar Carneiro, Jose Firmino Nogueira Neto, Fernanda Cristina C. Mattos Magno, Eliane Lopes Rosado, Lohanna Palhinha, Clarissa Menezes Maya-Monteiro, Giselda Maria Kalil de Cabello, Pedro Hernan Cabello, Patricia Torres Bozza, Veronica Marques Zembrzuski, Ana Carolina Proenca da Fonseca
FRONTIERS IN GENETICS
(2020)
Article
Obstetrics & Gynecology
Fabiana B. Kohlrausch, Fang Wang, Isaac Chamani, David L. Keefe
Summary: This study focuses on the possible relationship between telomere dysfunction and aneuploidy in human early embryo development, discussing the mechanisms of aneuploidy caused by telomere shortening and fusion in human embryos. Research suggests that telomere length plays an important role in the development of the embryo and fetus, and abnormal shortening of telomeres may lead to embryo loss during early human development. Complications of assisted reproductive technology pregnancies may also be linked to telomere shortening.
OBSTETRICAL & GYNECOLOGICAL SURVEY
(2021)
Review
Endocrinology & Metabolism
Kaio Cezar Rodrigues Salum, Jonatas de Mendonca Rolando, Veronica Marques Zembrzuski, Joao Regis Ivar Carneiro, Cicero Brasileiro Mello, Clarissa Menezes Maya-Monteiro, Patricia Torres Bozza, Fabiana Barzotto Kohlrausch, Ana Carolina Proenca da Fonseca
Summary: Obesity is a complex disease caused by environmental factors and genetic susceptibility, with leptin gene mutations potentially leading to disrupted bodily energy balance and abnormal immune response, but these phenotypes can be partially reversed by exogenous administration of leptin.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Thalita S. Berteli, Fang Wang, Fabiana B. Kohlrausch, Caroline M. Da Luz, Fernanda Oliveira, David L. Keefe, Paula A. Navarro
Summary: Superovulation and in vitro fertilization (IVF) may result in genomic changes, including altered telomere length and activation of retrotransposon LINE-1. This study found that embryos produced through superovulation and IVF had significantly longer telomeres compared to embryos produced naturally. The implications of this enriched telomere length for the health and lifespan of IVF offspring deserve further investigation.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Genetics & Heredity
Thalita S. Berteli, Fang Wang, Wilson McKerrow, Paula A. Navarro, David Fenyo, Jef D. Boeke, Fabiana B. Kohlrausch, David L. Keefe
Summary: This study uses a new sequencing method called scTIPseq to identify novel LINE-1 insertions in human sperm for the first time, demonstrating the feasibility of scTIPseq and identifying new contributors to genetic diversity in the human germline.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Genetics & Heredity
Thalita S. Berteli, Fang Wang, Paula A. Navarro, Fabiana B. Kohlrausch, David L. Keefe
Summary: Unlike other cells, sperm's telomere length increases with age. We found that as paternal age increases, telomere length increases and L1 copy number decreases significantly. Sperm with normal morphology have longer telomeres and normal and abnormal sperms have similar L1 copy numbers.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Respiratory System
Marcos Cesar Santos de Castro, Angela Santos Ferreira Nani, Kaio Cezar Rodrigues Salum, Jonatas de Mendonca Rolando, Julia Fernandes Barbosa dos Santos, Hermano Albuquerque de Castro, Patricia Canto Ribeiro, Walter Costa, Cicero Brasileiro de Mello, Fabiana Barzotto Kohlrausch
Summary: This study evaluated the influence of polymorphisms on the severity of silicosis and found that polymorphisms in the TGFB1 gene and FASLG gene are associated with the severity of silicosis.
JORNAL BRASILEIRO DE PNEUMOLOGIA
(2022)
Article
Reproductive Biology
Fabiana B. Kohlrausch, Fang Wang, Danxia Luo, Rebecca Mahn, David L. Keefe
Summary: The placenta plays a crucial role in supporting the fetus during pregnancy, but it shows signs of aging in late pregnancy, including inflammation and impaired function. This pilot study discovered abnormal chromosome fusion in the placenta of full-term pregnancies. Further research is needed to determine the impact of this finding on placental function and pregnancy outcomes.
REPRODUCTION AND FERTILITY
(2022)
Meeting Abstract
Obstetrics & Gynecology
Thalita S. Berteli, Fang Wang, Fabiana B. Kohlrausch, Caroline M. Da Luz, Fernanda V. Oliveira, David L. Keefe, Paula A. Navarro
FERTILITY AND STERILITY
(2021)
Meeting Abstract
Obstetrics & Gynecology
T. Berteli, F. Wang, W. McKerrow, P. Navarro, D. Fenyo, J. Boeke, F. Kohlrausch, D. Keefe
HUMAN REPRODUCTION
(2021)
Meeting Abstract
Obstetrics & Gynecology
Thalita S. Berteli, Fang Wang, Paula A. Navarro, Fabiana B. Kohlrausch, David L. Keefe
FERTILITY AND STERILITY
(2020)
Meeting Abstract
Obstetrics & Gynecology
Fabiana B. Kohlrausch, Fang Wang, Wilson McKerrow, David Fenyo, Jef D. Boeke, David L. Keefe
FERTILITY AND STERILITY
(2020)
