Article
Cell Biology
Laura Mainz, Mohamed A. F. E. Sarhan, Sabine Roth, Ursula Sauer, Charis Kalogirou, Markus Eckstein, Elena Gerhard-Hartmann, Helen-Desiree Seibert, Hans-Ulrich Voelker, Carol Geppert, Andreas Rosenwald, Martin Eilers, Almut Schulze, Markus Diefenbacher, Mathias T. Rosenfeldt
Summary: The potential therapeutic target of macroautophagy/autophagy for various diseases, including cancer, is mainly based on preclinical mouse studies. This study introduces a shRNA transgenic mouse model that allows simultaneous knockdown of Atg7 in most organs, providing a valuable tool for further understanding of the role of autophagy impairment at different disease stages.
Article
Biochemistry & Molecular Biology
Masaaki Ito, Takashi Yurube, Yutaro Kanda, Yuji Kakiuchi, Yoshiki Takeoka, Toru Takada, Ryosuke Kuroda, Kenichiro Kakutani
Summary: The study demonstrates that autophagy in human disc cells plays a protective role against cellular apoptosis and senescence, rather than affecting extracellular matrix degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Hua Zhu, Yi Zhong, Ran Chen, Lei Wang, Yuntao Li, Zhihong Jian, Lijuan Gu, Xiaoxing Xiong
Summary: This study aimed to investigate the role of autophagy-related gene ATG5 in ischemic stroke and found that ATG5 knockdown could attenuate autophagy-induced ferroptosis, providing a potential novel approach for ischemic stroke treatment.
TRANSLATIONAL STROKE RESEARCH
(2022)
Article
Fisheries
Xuemei Yuan, Jiayun Yao, Lei Huang, Lingyun Lin, Xiaoyi Pan, Jinbiao Jiao, Haiqi Zhang
Summary: The study finds that RNAi-based gene silencing can effectively inhibit the replication of MSRV virus in CO cells and largemouth bass larvae, providing a strategy to diminish the damages of viral disease in largemouth bass aquaculture.
Article
Cell Biology
Mehrdad Alirezaei, Claudia T. Flynn, Selma D. Garcia, Taishi Kimura, J. Lindsay Whitton
Summary: Food restriction in mice increases susceptibility to attenuated enterovirus infection, with TFEB playing a crucial role in regulating this effect. TFEB's rapid increase in expression and activation status under food restriction influences the host's susceptibility to coxsackievirus B3 infection.
Review
Biochemistry & Molecular Biology
Harish Changotra, Sargeet Kaur, Suresh Singh Yadav, Girdhari Lal Gupta, Jyoti Parkash, Ajay Duseja
Summary: Autophagy is a crucial process in maintaining cellular homeostasis, and ATG5 plays a central role in this process. In addition to its role in autophagy, ATG5 also has autophagy-independent functions and is associated with various diseases.
CELL BIOCHEMISTRY AND FUNCTION
(2022)
Article
Biochemistry & Molecular Biology
Ting Sun, Hui Peng, Wenhao Mao, Liwei Ma, Hongyang Liu, Jia Mai, Lin Jiao
Summary: YAP and autophagy interact in PDAC, where YAP activates autophagy and autophagy negatively regulates YAP through degradation. Hyperactivation of YAP disrupts the YAP-autophagy circuit, promoting cancer progression in PDAC.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Liuwei Zhang, Jingyun Wang, Hongyan Cui, Haonan Zheng, Xiaolan Yin, Jiaqi Lin, Yue Wang, Yan Zhao, Haidong Li, Qixian Chen
Summary: Tumors that can escape immune clearance can be effectively treated by a chemotherapeutic nanomedicine that targets the tumors and delivers siRNA payloads to knock down immune suppressive molecules, resulting in the reactivation of anti-tumor immunities. This study demonstrated significant anti-tumor effects in triple-negative breast tumors by using a chemotherapeutic nanomedicine that combines targeted delivery and RNA interference.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Cell Biology
Suna Zhou, Xuequan Wang, Jiapei Ding, Haihua Yang, Youyou Xie
Summary: This study revealed that ATG5 plays a crucial role in regulating EMT and predicting poor prognosis in cervical cancer. The survival rate of cervical cancer patients was influenced by ATG5, and functional experiments confirmed its importance in the EMT process. Analysis of TCGA data and functional enrichment results highlighted the significant association of ATG5 with cancer-related pathways in cervical cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Liuwei Zhang, Jingyun Wang, Hongyan Cui, Haonan Zheng, Xiaolan Yin, Jiaqi Lin, Yue Wang, Yan Zhao, Haidong Li, Qixian Chen
Summary: Targeted therapy, inhibition of immune suppressive molecules, and the use of chemotherapy drugs can improve anti-tumor outcomes.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Cell Biology
Fulong Wang, Einar S. Trosdal, Masroor Ahmad Paddar, Thabata L. A. Duque, Lee Allers, Michal Mudd, Prithvi R. Akepati, Ruheena Javed, Jingyue Jia, Michelle Salemi, Brett Phinney, Vojo Deretic
Summary: ATG5 plays a crucial role in membrane Atg8ylation and has negative effects on tuberculosis in myeloid cells. The absence of ATG5 leads to a lysosomal hypersensitivity phenotype in endolysosomal organelles, partially due to compromised recruitment of ESCRT proteins for lysosomal repair. This finding is of significance for understanding tuberculosis pathogenesis.
Review
Cell Biology
Anna Kotowska-Zimmer, Marianna Pewinska, Marta Olejniczak
Summary: RNA interference (RNAi) technology has been used for studying gene functions and therapeutic approaches, with amiRNAs showing the most complex structures. AmiRNAs can form mature siRNAs through two-step processing in cells, have dynamics similar to endogenous miRNAs, and are considered safer than other RNAi triggers.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2021)
Article
Medicine, General & Internal
Wan-Hsiang Hu, Wen-Chi Yang, Pei-Feng Liu, Ting-Ting Liu, Paul Morgan, Wei-Lun Tsai, Hung-Wei Pan, Cheng-Hsin Lee, Chih-Wen Shu
Summary: Gene mutation and pathogenesis bacteria are highly associated with colorectal cancer (CRC) development and progression. Autophagy, especially the key component ATG5, plays a crucial role in CRC malignancy with implications for both overall and disease-free survival. Further studies on ATG5 may provide insights into its potential as a prognostic biomarker and therapeutic target for CRC patients.
Article
Cell Biology
Mingjun Shi, Sierra Shepard, Zhiyong Zhou, Jenny Maique, Olivia Seli, Orson W. Moe, Ming Chang Hu
Summary: High phosphate contributes to uremic cardiomyopathy by suppressing autophagy activity, while high phosphate and low autophagy independently trigger and additionally amplify cardiac remodeling and dysfunction.
Review
Chemistry, Analytical
Marie Isenmann, Martin James Stoddart, Rainer Schmelzeisen, Christian Gross, Elena Della Bella, Rene Marcel Rothweiler
Summary: Since its discovery in 1989, RNA interference (RNAi) has become a widely used tool for downregulating specific gene expression in molecular biological research. Different small RNAs and delivery methods are available for in vitro cell culture to regulate gene expression by mimicking RNAi-machinery. Understanding their basic mode of action is crucial due to different off-target effects and limitations.
