Editorial Material
Biochemistry & Molecular Biology
Christophe Viret, Mathias Faure
Summary: ATG8 proteins are core components of autophagy, decorating autophagosomes and organelles. Recent studies show that the membrane anchoring of single-membrane associated ATG8 proteins may involve phosphatidylserine conjugation, and their stability depends on ATG4 protease inhibition.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Article
Cell Biology
Tomasz K. Prajsnar, Justyna J. Serba, Bernice M. Dekker, Josie F. Gibson, Samrah Masud, Angeleen Fleming, Simon A. Johnston, Stephen A. Renshaw, Annemarie H. Meijer
Summary: The study reveals that intracellular autophagy in neutrophils may have both beneficial and detrimental effects on hosts infected with Staphylococcus aureus, with different pathways leading to conflicting outcomes.
Article
Food Science & Technology
Aina Ichihara, Hinako Ojima, Kazuyoshi Gotoh, Osamu Matsushita, Susumu Take, Hiroyuki Okada, Akari Watanabe, Kenji Yokota
Summary: This study aimed to explore the association between different antibody reactivities and bacterial genome organization. High-reactive strains showed high gene homology, while inversion around the vacA gene was found in the genome of poorly responsive antigen strains.
Article
Chemistry, Multidisciplinary
Yiqing Zou, Xiaonan Chen, Yingying Sun, Pengyu Li, Mao Xu, Pengchao Fang, Shuqi Zhang, Gang Yuan, Xin Deng, Haiyan Hu
Summary: In this study, fucoidan-coated nanoparticles were developed to target and destroy Helicobacter pylori biofilms, as well as kill the bacteria inside host cells. These nanoparticles not only improved bacterial eradication but also alleviated oxidative stress and reduced gastric mucosal damage.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Multidisciplinary Sciences
Tohid Piri-Gharaghie, Ghazal Ghajari, Shakiba Tolou-Shikhzadeh-Yazdi, Mona Aghassizadeh-Sherbaf, Sahar Khorsand-Dehkordi
Summary: Despite being a serious pathogen, the origin of Helicobacter pylori (H. pylori) remains unknown. Poultry, such as chicken and turkey, is widely consumed as a source of protein worldwide, making it important to ensure sanitary practices in poultry delivery for food. This study examined the distribution of pathogenicity and antimicrobial resistance in H. pylori isolates from poultry meat. The findings highlight the presence of H. pylori in raw poultry samples and the prevalence of antibiotic-resistant genotypes, raising concerns about public health risks associated with consuming raw poultry.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Li Wang, Juan Yi, Xiao-Yang Yin, Jin-Xia Hou, Jing Chen, Bei Xie, Gang Chen, Qun-Feng Wang, Li-Na Wang, Xiao-Yuan Wang, Jing Sun, Lei-Ming Huo, Tuan-Jie Che, Hu-Lai Wei
Summary: The study revealed that Helicobacter pylori's VacA induces autophagy in gastric epithelial cells and promotes mitophagy by damaging mitochondrial membrane potential, leading to PINK1 accumulation and Parkin recruitment.
FRONTIERS IN ONCOLOGY
(2022)
Review
Medical Laboratory Technology
Salika Mehreen Shakir, Faiz Ahmed Shakir, Marc Roger Couturier
Summary: This review provides a summary of the prevalence and microbiology of H. pylori, as well as current diagnostic methods and clinical management strategies. The advantages of molecular methods for diagnosis and predicting drug resistance are discussed. The gold standard for detecting resistance patterns is culture-based diagnostics followed by antimicrobial susceptibility testing.
CLINICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Jinfeng Dai, Jing Zhao, Liqi Mao, Yue Hu, Bin Lv
Summary: The aim of this study was to explore the value of detecting antibiotic-resistant genes in Helicobacter pylori (H. pylori) and to investigate the association between genotype and antibiotic resistance. The results showed that the detection of related mutant genes had a clinical application value in predicting the antibiotic resistance of H. pylori, particularly resistance to clarithromycin and levofloxacin.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Review
Pharmacology & Pharmacy
Romelia Pop, Alexandru-Flaviu Tabaran, Andrei Paul Ungur, Andrada Negoescu, Cornel Catoi
Summary: Helicobacter pylori is a leading bacterial carcinogen and the main cause of gastroduodenal diseases. The high rates of antimicrobial drug resistance pose challenges to current therapies, making the exploration of new treatment options necessary. Silver nanoparticles offer advantages in improving H. pylori therapy and overcoming drug resistance.
Article
Medicine, Research & Experimental
Patrick F. Asare, Plinio R. Hurtado, Hai B. Tran, Griffith B. Perkins, Eugene Roscioli, Sandra Hodge
Summary: A common feature of COPD is defective lung macrophage phagocytic capacity. This study investigated the molecular basis by which cigarette smoke extract (CSE) reduces Rubicon expression in macrophages and its relationship with impaired phagocytosis. The results showed that CSE decreases Rubicon through lysosomal degradation, which may lead to dysregulated phagocytosis.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
Review
Infectious Diseases
Mohsen Karbalaei, Amin Talebi Bezmin Abadi, Masoud Keikha
Summary: This study found a significant association between cagA-positive strains of H. pylori and increased resistance to metronidazole. In Western populations, infection with cagA-positive strains may lead to increased resistance to metronidazole, amoxicillin, and levofloxacin. On the other hand, vacA genotypes usually decrease antibiotic resistance in H. pylori, with vacA s1m1 significantly reducing resistance to metronidazole.
BMC INFECTIOUS DISEASES
(2022)
Article
Immunology
Sebastian Reuter, Jonas Raspe, Hendrik Uebner, Alexandros Contoyannis, Eva Pastille, Astrid M. Westendorf, Georgia C. Caso, Timothy L. Cover, Anne Mueller, Christian Taube
Summary: This study evaluated the therapeutic efficacy of Helicobacter pylori-derived VacA in allergic airway inflammation and found that VacA suppressed allergic airway inflammation by inducing regulatory T cells (Tregs). These findings suggest that the immunomodulatory activity of VacA could be potentially used for the prevention and treatment of allergic airway disease.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Julia Juelg, Laura Strohm, Christian Behrends
Summary: Autophagy is a major degradation pathway within cells, contributing to the maintenance of organelle, protein, and metabolite homeostasis as well as innate immunity. In addition to canonical autophagy, there exists a noncanonical form. Studies have shown that dysfunction in autophagy is associated with several human pathologies, including cancer, immune diseases, and neurodegenerative disorders.
MOLECULAR AND CELLULAR BIOLOGY
(2021)
Article
Immunology
Wenhui Chen, Zhen Liu, Ying Zheng, Bo Wei, Jingdong Shi, Baowei Shao, Di Wang
Summary: The study found that selenium is associated with pulmonary tuberculosis infection and may function through proinflammatory and autophagy pathways; In in vivo experiments, selenium was shown to combat MTB by enhancing macrophage autophagy related pathways.
MICROBIAL PATHOGENESIS
(2021)
Article
Cell Biology
Sasikala Muthusamy, Hau-Ming Jan, Ming-Yen Hsieh, Soumik Mondal, Wen-Chun Liu, Yi-An Ko, Wei-Yuan Yang, Kwok-Kong Tony Mong, Guang-Chao Chen, Chun-Hung Lin
Summary: The study revealed that CAG enhances the internalization of H. pylori in host cells, disrupts autophagy flux, and reduces lysosomal biogenesis.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Article
Multidisciplinary Sciences
An N. T. Nguyen, Laura C. Woods, Rebecca Gorrell, Shamitraa Ramanan, Terry Kwok, Michael J. McDonald
Summary: Horizontal gene transfer is vital for microbial evolution, yet we have limited knowledge about the fitness effects and dynamics of these transferred genetic variants. Through evolutionary experiments on laboratory populations of Helicobacter pylori, which naturally take up DNA from their environment, we measured the fitness effects of thousands of transferred genetic variants. Our findings reveal that natural transformation increases the rate of adaptation but comes with a significant genetic load. However, recombination bypasses this cost by enhancing selection efficiency through separating harmful and beneficial genetic variants. Our results demonstrate that adaptation with horizontal gene transfer, which is pervasive in natural microbial populations, is influenced by a combination of selection, recombination, and genetic drift that existing evolutionary models fail to account for.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Nutrition & Dietetics
Yonghui Dong, Xue Wu, Lin Han, Ji Bian, Caian He, Emad El-Omar, Lan Gong, Min Wang
Summary: Cardiovascular disease is a leading global health issue, and anthocyanins have been shown to have antioxidant, anti-inflammatory, and anti-aging effects, providing protection against cardiovascular diseases.
Review
Nutrition & Dietetics
Kailin Li, Yao Xiao, Ji Bian, Lin Han, Caian He, Emad El-Omar, Lan Gong, Min Wang
Summary: Urolithin A (Uro A) is a dietary metabolite with potential health benefits in various diseases. It has been found to improve pancreatic diseases by activating autophagy and inhibiting endoplasmic reticulum stress, resulting in reduced oxidative stress, inflammation, and apoptosis.
Article
Nutrition & Dietetics
Zhixin Luo, Mengqian Li, Qiong Yang, Yuhong Zhang, Fang Liu, Lan Gong, Lin Han, Min Wang
Summary: Ferulic acid (FA) has a protective effect on high-fat diet-induced non-alcoholic fatty liver disease (NAFLD) by activating PPARα to decrease triacylglycerol accumulation and increase energy expenditure.
Article
Multidisciplinary Sciences
Jingjing Jiang, Yuyu Li, Zeyu Sun, Lan Gong, Xuehui Li, Fan Shi, Jian Yao, Yuting Meng, Xiaohua Meng, Qiong Zhang, Yuchong Wang, Xiaoling Su, Hongyan Diao
Summary: This study discovered that lncNSPL is highly expressed in monocytes of patients infected with influenza A virus (IAV). By blocking the interaction between RIG-I and TRIM25, lncNSPL limits the production of antiviral mediators, promoting IAV replication and immune evasion.
Article
Microbiology
Jianing Chen, Ping Zeng, Lan Gong, Xujun Zhang, Zongxin Ling, Kefan Bi, Fan Shi, Kaihang Wang, Qiong Zhang, Jingjing Jiang, Yanhui Zhang, Toshimitsu Uede, Emad M. El-Omar, Hongyan Diao
Summary: The study found that osteopontin (OPN) levels were elevated in individuals with obesity compared to healthy controls, with a positive correlation between OPN levels and body mass index (BMI). OPN exacerbated high-fat diet-induced metabolic disorders and gut dysbiosis. OPN regulated the gut microbiota by decreasing the adhesion of Lactobacillus to intestinal epithelial cells.
Editorial Material
Medicine, General & Internal
Ding Shi, Silvia Turroni, Lan Gong, Wenrui Wu, Howard Chi Ho Yim
FRONTIERS IN MEDICINE
(2022)
Article
Nutrition & Dietetics
Zhixin Luo, Mengqian Li, Jiachuan Yang, Jia Li, Yao Zhang, Fang Liu, Emad El-Omar, Lin Han, Ji Bian, Lan Gong, Min Wang
Summary: This study found that ferulic acid (FA) can reduce high cholesterol levels caused by a high-fat diet. It does so by increasing the excretion of bile acids in the feces. FA activates the enzyme CYP7A1 in the liver, which biosynthesizes bile acids from cholesterol. This new mechanism discovered in the study may be related to the anti-obesity and hypoglycemic effects of FA.
FRONTIERS IN NUTRITION
(2022)
Article
Virology
Fan Zhang, Andrew Gia, Guowei Chen, Lan Gong, Jason Behary, Georgina L. Hold, Amany Zekry, Xubo Tang, Yanni Sun, Emad El-Omar, Xiao-Tao Jiang
Summary: Viruses are abundant and important in ecosystems, and the two methods of whole genome shotgun metagenome and viral-like particle enriched metagenome sequencing are commonly used for comparing viruses in different environments. In this study, both methods were applied to investigate the stool virome in HCC patients and healthy controls, and they both successfully identified altered viral profiles in HCC patients. Ultra-deep sequencing recovered more viruses, and VLPM method can detect RNA viruses. Using both methods would capture different parts of the total virome and identify shared and specific viral signatures.
Review
Biochemistry & Molecular Biology
Kailin Li, Ji Bian, Yao Xiao, Da Wang, Lin Han, Caian He, Lan Gong, Min Wang
Summary: In recent years, there has been a significant increase in age-related diseases worldwide due to longer life expectancy. The aging pancreas undergoes various morphological and pathological changes, which can predispose individuals to diseases such as diabetes, dyspepsia, pancreatic ductal adenocarcinoma, and pancreatitis. Pancreatic senescence is associated with underlying factors including genetic damage, DNA methylation, endoplasmic reticulum stress, mitochondrial dysfunction, and inflammation. This review article discusses the alterations in morphologies and functions of the aging pancreas, particularly beta-cells, and summarizes the mechanisms of pancreatic senescence to identify potential targets for treating aging-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mingsheng Cai, Bin Xiao, Yuanfang Wang, Kezhen Wang, Wenqi Luo, Jiangqin Fu, Shuai Wang, Shenyu Deng, Bolin Li, Lan Gong, Jiayi Zhong, Li Hu, Lingxia Pan, Liding Wang, Yintao Liu, Chen Huang, Xiaoqing Li, Qiyuan Zeng, Haoran Kang, Linhai Li, Jie Zan, Tao Peng, Haidi Yang, Meili Li
Summary: In this study, it was found that the surface protein gp110 of Epstein-Barr virus (EBV) plays an important role in regulating the host's innate immunity. Gp110 inhibits NF-KB promoter activity and the production of NF-KB-regulated cytokines. It interacts with the transcription factor p65, limiting its activation and nuclear translocation. These findings suggest that gp110 is a vital component for EBV to antagonize the host's innate immune response and contribute to the understanding of EBV infectivity and pathogenesis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Yao Xiao, Kailin Li, Ji Bian, Yao Zhang, Jia Li, Hang Liu, Yingzhi Ye, Lin Han, Lan Gong, Min Wang
Summary: This study investigates the effects and mechanisms of Urolithin A (UA) on cognitive impairment in a mouse model of type 2 diabetes mellitus (T2DM) induced by high-fat diet and streptozotocin. The results show that UA can attenuate cognitive impairment by suppressing endoplasmic reticulum stress and Tau hyperphosphorylation in the brain. UA also reduces oxidative stress, abnormal energy metabolism, and apoptosis in damaged neuronal cells. Atp2a3 is identified as a potential target gene of UA that plays a role in intracellular calcium homeostasis, ER stress, and apoptosis. UA's protective effects against ER stress and apoptosis are abolished by over-expression of Atp2a3. Overall, this study suggests that UA could be a potential therapeutic target for neurodegeneration and stress damage by regulating Atp2a3 expression.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Meeting Abstract
Oncology
Wenwen Zhu, Ningning Zhang, Zeyu Wang, Shuwen Zhang, Xiaofen Yue, Linlin Sun, Jiao Sun, Lan Gong, Emad M. El-Omar, Wei Lu
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Ningning Zhang, Zeyu Wang, Jiayu Lv, Yang Liu, Tian Liu, Shuwen Zhang, Wang Li, Lan Gong, Xiaodong Zhang, Emad El-Omar, Wei Lu
JOURNAL OF HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alexander Bujotzek, Georg Tiefenthaler, Laurent Lariviere, Laura D'Andrea, Elsa A. Marquez, Ina Rudloff, Steven X. Cho, Nadia S. Deen, Wolfgang Richter, Franziska Regenass-Lechner, Alexander Poehler, James C. Whisstock, Jasmin Sydow-Andersen, Xaver Reiser, Sabine Schuster, Jeannette Neubauer, Sebastian Hoepfl, Kirsten Richter, Marcel F. Nold, Claudia A. Nold-Petry, Felix Schumacher, Andrew M. Ellisdon
Summary: This article presents the engineering of the unstable cytokine IL-37 into an anti-inflammatory molecule with excellent therapeutic properties. The use of site-directed mutagenesis, the addition of a non-native disulfide bond, and the engineering of IL-37 as an Fc-fusion protein overcame its poor pharmacokinetic and manufacturing profile. These findings provide a platform for preclinical testing of IL-37 Fc-fusion proteins and can be applied to convert similar short-acting cytokines into therapeutics.
CELL CHEMICAL BIOLOGY
(2022)