4.6 Article

Collision cross sections of high-mannose N-glycans in commonly observed adduct states - identification of gas-phase conformers unique to [M - H](-) ions

Journal

ANALYST
Volume 14, Issue 20, Pages 6799-6803

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c5an01092f

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Funding

  1. Biotechnology and Biological Sciences Research Council (BBSRC) [BB/L017733/1]
  2. Free University Berlin
  3. Fritz Haber Institute of the Max Planck Society
  4. BBSRC [BB/L017733/1] Funding Source: UKRI
  5. Biotechnology and Biological Sciences Research Council [BB/L017733/1] Funding Source: researchfish

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We report collision cross sections (CCS) of high-mannose N-glycans as [M + Na](+), [M + K](+), [M + H](+), [M + Cl](-), [M + H2PO4](-) and [M -H](-) ions, measured by drift tube (DT) ion mobility-mass spectrometry (IM-MS) in helium and nitrogen gases. Further analysis using traveling wave (TW) IM-MS reveal the existence of distinct conformers exclusive to [M -H](-) ions.

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