Article
Endocrinology & Metabolism
Xiaoxiao Sun, Linling Xu, Ying Xia, Shuoming Luo, Jian Lin, Yang Xiao, Gan Huang, Xia Li, Zhiguo Xie, Zhiguang Zhou
Summary: This study found that rs3806265 and rs4612666 of the NLRP3 gene are significantly associated with GADA titers in Chinese Han T1D patients.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Immunology
Joseph R. Podojil, Samantha Genardi, Ming-Yi Chiang, Sandeep Kakade, Tobias Neef, Tushar Murthy, Michael T. Boyne, Adam Elhofy, Stephen D. Miller
Summary: Type 1 diabetes is an autoimmune disease that can be effectively inhibited by encapsulating multiple diabetogenic proteins in nanoparticles, which induce regulatory T cells and decrease inflammation in pancreatic islets.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Carina Torn, Xiang Liu, Suna Onengut-Gumuscu, Kevin M. Counts, Jose Leonardo Moreno, Cassandra L. Remedios, Wei-Min Chen, Jonathon LeFaive, Martha D. Butterworth, Beena Akolkar, Jeffrey P. Krischer, Ake Lernmark, Marian Rewers, Jin-Xiong She, Jorma Toppari, Anette-Gabriele Ziegler, Aakrosh Ratan, Albert Smith, William A. Hagopian, Stephen S. Rich, Hemang M. Parikh
Summary: The TEDDY study found that the age at blood sample collection was negatively correlated with telomere length, European children had shorter telomeres than American children, paternal age was positively associated with telomere length, and maternal age, gestational diabetes, and first-degree relative status did not have a significant impact on telomere length. Additionally, children with HLA-DR4/4 or HLA-DR4/X genotypes had longer telomeres. The study also found that telomere length did not significantly affect the risk for islet autoimmunity or type 1 diabetes, but was shorter in high prevalence populations in Finland and Sweden.
SCIENTIFIC REPORTS
(2022)
Article
Endocrinology & Metabolism
Angus G. Jones, Timothy J. McDonald, Beverley M. Shields, William Hagopian, Andrew T. Hattersley
Summary: LADA, diagnosed after age 35, presents clinical features similar to type 2 diabetes but with type 1 diabetes-associated islet autoantibodies. The current definition of LADA identifies a group with features intermediate between typical type 1 and type 2 diabetes, leading to difficulties in classification. Improved approaches are needed to increase test specificity and better understand the detailed pathophysiological components of late-onset autoimmune diabetes.
Article
Medical Laboratory Technology
Feliciana Real-Fernandez, Alessandra Gallo, Francesca Nuti, Lorenzo Altamore, Gloria Giovanna Del Vescovo, Pietro Traldi, Eugenio Ragazzi, Paolo Rovero, Annunziata Lapolla, Anna Maria Papini
Summary: The study investigated the use of antibodies against human glutamic acid decarboxylase (hGAD) peptides for diagnosing latent autoimmune diabetes in adults (LADA) and type 1 diabetes mellitus (T1DM), showing high diagnostic predictive power of IgM antibodies in LADA. Results highlighted the utility of peptides as diagnostic antigens in T1DM and LADA, and emphasized the importance of comparing IgM and IgG-subtype antibodies in the same population.
CLINICA CHIMICA ACTA
(2021)
Article
Endocrinology & Metabolism
Dhekra Al-Zubairi, Molham AL-Habori, Riyadh Saif-Ali
Summary: This study investigated the prevalence and metabolic characteristics of latent autoimmune diabetes in adults (LADA) among Yemeni Type 2 DM patients. The results showed that LADA patients had different characteristics in certain biochemical parameters and a lower prevalence of metabolic syndrome compared to Type 2 DM.
DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY
(2021)
Article
Medicine, General & Internal
Hui-Ju Chen, Yann-Jinn Lee, Chao-Ching Huang, Yuh-Feng Lin, Sung-Tse Li
Summary: This study found that children with type 1 diabetes had lower serum BDNF levels and poorer neurocognitive function, especially those with GAD65-Ab positive diabetes. Poor glycemic control was correlated with worse cognitive performance.
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION
(2021)
Article
Biochemistry & Molecular Biology
Tomislav Bulum, Marijana Vucic Lovrencic, Jadranka Knezevic Cuca, Martina Tomic, Sandra Vuckovic-Rebrina, Lea Duvnjak
Summary: This study aimed to investigate the relationship between three autoantibodies and their combination with anthropometric and metabolic components and microvascular complications in patients with latent autoimmune diabetes in adults (LADA). The results showed that ZnT8 and IA-2 autoantibodies were present in a significant number of LADA patients and associated with clinical and metabolic characteristics resembling classic type 1 diabetes.
Article
Endocrinology & Metabolism
Isilay Taskaldiran, Asli Nar
Summary: This article reports a case of newly developed autoimmune Type 1 diabetes in an adult patient after SARS-CoV-2 infection. The case highlights the importance of preventive measures, such as vaccination, to protect against COVID-19 and its complications.
ENDOCRINE METABOLIC & IMMUNE DISORDERS-DRUG TARGETS
(2023)
Article
Endocrinology & Metabolism
Barbara Brooks-Worrell, Christiane S. Hampe, Erica G. Hattery, Brenda Palomino, Sahar Z. Zangeneh, Kristina Utzschneider, Steven E. Kahn, Mary E. Larkin, Mary L. Johnson, Kieren J. Mather, Naji Younes, Neda Rasouli, Cyrus Desouza, Robert M. Cohen, Jean Y. Park, Hermes J. Florez, Willy Marcos Valencia, Ali Shojaie, Jerry P. Palmer, Ashok Balasubramanyam
Summary: The prevalence of islet autoimmunity in patients with type 2 diabetes is higher than previously reported. T-cell-mediated autoimmunity is associated with reduced beta-cell function and poorer glycemic control.
Article
Medicine, General & Internal
Quynh Thi Vu Huynh, Minh Thi Tuyet Trinh, Khang Kim Doan, Ban Tran Ho, Szu-Chuan Shen, Tung Huu Trinh, Thanh Hoa Vo, Nguyen Quoc Khanh Le, Ngan Thi Kim Nguyen
Summary: Asian children in Vietnam are being diagnosed with both type 1 and type 2 diabetes, and the presence of islet autoantibodies makes the diagnosis more complicated. A study found that the prevalence of islet cell autoantibodies (ICAs) and glutamic acid decarboxylase 65 autoantibodies (GADAs) in children with type 1 diabetes was not significantly different from those with type 2 diabetes. Older children with type 1 diabetes were more likely to have ICAs or ICAs and GADAs, while a small proportion of young children had GADAs. However, a significant number of older children with type 2 diabetes were positive for GADAs, and all of them were classified as overweight or obese. The study suggests that further investigation is needed to find better biomarkers or an appropriate time to confirm diabetes type.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Eiji Kawasaki
Summary: Anti-islet autoantibodies are crucial markers for immune-mediated type 1 diabetes (T1D) and slowly progressive T1D (SPIDDM) or latent autoimmune diabetes in adults (LADA). They are used for diagnosis, pathological analysis, and prediction of T1D. Different autoantibodies serve different purposes, such as reflecting insulitis or pancreatic & beta-cell destruction. Combinatorial analysis of these autoantibodies helps diagnose immune-mediated T1D, while a lack of autoantibodies is associated with fulminant T1D. Evaluating the epitopes and subclasses of these autoantibodies aids in distinguishing diabetes-associated from non-diabetes-associated autoantibodies and predicting future insulin deficiency. New advancements in autoantibody assays can improve the accuracy of diagnosis and prediction of autoimmune disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Jing Li, Songbai Lin, Chuiwen Deng, Tengda Xu
Summary: The prevalence of GADA in the general adult population in China is low but is associated with a 2.63-4.16-fold increased risk for diabetes. This increased risk is particularly significant in male adults.
DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY
(2021)
Article
Chemistry, Analytical
Lijie Ren, Zhigang Zhang, Xinyuan Zhang, Zhibo Kong, Shaowei Li, Qinjian Zhao
Summary: By utilizing a fluorescence imaging-based high content analysis, this study analyzed the interactions of protein molecules on particle surfaces, demonstrating the feasibility of analyzing subtle conformational changes in antigen epitopes. The method is highly amenable for automation and is an ideal choice for formulation development and stability testing of products.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Multidisciplinary Sciences
Aulanni'am Aulanni'am, Dyah Kinasih Wuragil, Hendra Susanto, Anita Herawati, Yulianto Muji Nugroho, Wahyu Nur Laili Fajri, Perdana Finawati Putri, Susiati Susiati, Jerry Dwi Trijoyo Purnomo, Ahmad Taufiq, Djoko Wahono Soeatmadji
Summary: This study developed a novel rapid test for anti-GAD(65) autoantibodies in type 1 diabetes patients, showing higher sensitivity, specificity, and correlation with HDL levels and age. The test demonstrated potential as a future clinical diagnostic kit for managing type 1 DM patients.
Article
Multidisciplinary Sciences
Xingchen Chen, Blake T. Riley, Simon J. de Veer, David E. Hoke, Jessica Van Haeften, Darren Leahy, Joakim E. Swedberg, Maria Brattsand, Perry J. Hartfield, Ashley M. Buckle, Jonathan M. Harris
Article
Biochemistry & Molecular Biology
Blake T. Riley, Olga Ilyichova, Simon J. de Veer, Joakim E. Swedberg, Emily Wilson, David E. Hoke, Jonathan M. Harris, Ashley M. Buckle
Article
Chemistry, Analytical
Jiaul Islam, Blake T. Riley, Christian Fercher, Martina L. Jones, Ashley M. Buckle, Christopher B. Howard, Rosalind P. Cox, Toby D. M. Bell, Stephen Mahler, Simon R. Corrie
ANALYTICAL CHEMISTRY
(2019)
Article
Endocrinology & Metabolism
Daniel E. Williams, Sarah N. Le, David E. Hoke, Peter G. Chandler, Monika Gora, Marlena Godlewska, J. Paul Banga, Ashley M. Buckle
Review
Endocrinology & Metabolism
Marlena Godlewska, Damian Gawel, Ashley M. Buckle, J. Paul Banga
HORMONE AND METABOLIC RESEARCH
(2019)
Review
Cell Biology
Peter G. Chandler, Ashley M. Buckle
Article
Biochemical Research Methods
James Fodor, Michael Brand, Rebecca J. Stones, Ashley M. Buckle
BMC BIOINFORMATICS
(2020)
Article
Biochemistry & Molecular Biology
Christopher Szeto, Joseph I. Bloom, Hannah Sloane, Christian A. Lobos, James Fodor, Dhilshan Jayasinghe, Demetra S. M. Chatzileontiadou, Emma J. Grant, Ashley M. Buckle, Stephanie Gras
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Deborah L. Burnett, Peter Schofield, David B. Langley, Jennifer Jackson, Katherine Bourne, Emily Wilson, Benjamin T. Porebski, Ashley M. Buckle, Robert Brink, Christopher C. Goodnow, Daniel Christ
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Peter G. Chandler, Li Lynn Tan, Benjamin T. Porebski, James S. Green, Blake T. Riley, Sebastian S. Broendum, David E. Hoke, Robert J. Falconer, Trent P. Munro, Malcolm Buckle, Colin J. Jackson, Ashley M. Buckle
Summary: The FN3Con monobody is explored as a potential alternative to antibodies for therapeutic applications, due to its extreme stability and binding capabilities to therapeutic targets. Grafting binding loops from Adnectin-anti-VEGFR2 onto the prestabilized FN3Con scaffold resulted in a construct with high affinity binding to VEGFR2, maintaining both stability and activity. This strategy offers promise for the development of monobody domains with desirable biophysical characteristics.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Matthew A. Spence, Matthew D. Mortimer, Ashley M. Buckle, Bui Quang Minh, Colin J. Jackson
Summary: This study used a structural alignment of diverse serpins to generate a comprehensive 6,000-sequence phylogeny, showing extensive diversification of the superfamily into many novel functional clades. Analysis indicated that the hub proteins are ancient and similar due to convergent evolution, rather than horizontal gene transfer as previously speculated. This work clarifies longstanding questions in the evolution of serpins and provides new directions for research in the field of serpin biology.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Sebastian S. Broendum, Daniel E. Williams, Brooke K. Hayes, Felix Kraus, James Fodor, Ben E. Clifton, Anne Geert Volbeda, Jeroen D. C. Codee, Blake T. Riley, Nyssa Drinkwater, Kylie A. Farrow, Kirill Tsyganov, Ryan D. Heselpoth, Daniel C. Nelson, Colin J. Jackson, Ashley M. Buckle, Sheena McGowan
Summary: The study identified the polyrhamnose backbone of Group A streptococcal cell wall as the binding target of PlyC, and characterized the putative rhamnose binding groove of PlyC with critical residues. The interaction between PlyC and the cell wall may be a high avidity one rather than a high-affinity one, contributing to PlyC's remarkable lytic activity.
MOLECULAR MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Xiyuan Bai, Ashley M. Buckle, Eszter K. Vladar, Edward N. Janoff, Reeti Khare, Diane Ordway, David Beckham, Lorelenn B. Fornis, Abraham Majluf-Cruz, Randolph Fugit, Brian M. Freed, Soohyun Kim, Robert A. Sandhaus, Edward D. Chan
Summary: This study found that enoxaparin enhances the inhibition of TMPRSS2 activity by alpha-1-antitrypsin, leading to the inhibition of coronavirus infection. Molecular modeling revealed the specific conformation of AAT and the role of negatively-charged heparin in this process.
SCIENTIFIC REPORTS
(2022)
Correction
Multidisciplinary Sciences
Xiyuan Bai, Ashley M. Buckle, Eszter K. Vladar, Edward N. Janoff, Reeti Khare, Diane Ordway, David Beckham, Lorelenn B. Fornis, Abraham Majluf-Cruz, Randolph V. Fugit, Brian M. Freed, Soohyun Kim, Robert A. Sandhaus, Edward D. Chan
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Meri Ayurini, Peter G. Chandler, Paul D. O'Leary, Ruoxin Wang, David Rudd, Karen D. Milewska, Lara R. Malins, Ashley M. Buckle, Joel F. Hooper
Summary: Cobalt-mediated radical polymerizations (CMRPs) have been achieved through the radical decarboxylation of tetrachlorophthalimide activated esters. This method allows for controlled radical polymerization of activated monomers across a wide temperature range using a single cobalt species, while incorporating polymer end groups derived from simple carboxylic acid derivatives and terminating with an organozinc reagent.