Article
Environmental Sciences
Andre C. Pereira, Ana Tenreiro, Monica Cunha
Summary: The combination of FISH and FLOW techniques allows for high-throughput evaluation and sorting of single cells within microbial communities in environmental microbiology, providing a key tool for studying their heterogeneity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Biochemistry & Molecular Biology
Rosana Rodriguez-Casuriaga, Adriana Geisinger
Summary: Flow cytometry has played a crucial role in the study of spermatogenesis in mammals, enabling the analysis of testicular heterogeneity and purification of specific stage spermatogenic cell populations. Through flow cytometry, researchers can gain insights into the molecular basis of spermatogenesis, including characterization of mutant phenotypes, transcriptomics, epigenetics, genome-wide chromatin studies, and the establishment of cell culture systems for research and clinical purposes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Simon Heumos, Sandra Dehn, Konstantin Braeutigam, Marius C. Codrea, Christian M. Schuerch, Ulrich M. Lauer, Sven Nahnsen, Michael Schindler
Summary: This study identified specific cell surface receptor biomarkers for colon and renal cancer through analyzing the surface receptors of tumor cells, with CD106 (VCAM1) identified as a promising novel immunotherapeutic target for renal cancer treatment.
CANCER CELL INTERNATIONAL
(2022)
Article
Cell Biology
Nicola Schanda, Tim Sauer, Alexander Kunz, Angela Huckelhoven-Krauss, Brigitte Neuber, Lei Wang, Mandy Hinkelbein, David Sedloev, Bailin He, Maria-Luisa Schubert, Carsten Muller-Tidow, Michael Schmitt, Anita Schmitt
Summary: CAR-T cells are revolutionizing cancer immunotherapy and monitoring their expansion and persistence in patients is crucial. Flow cytometry and qPCR are effective methods for quality control of CAR-T cell products, with preference for CAR detection reagents with low background staining for monitoring CAR-T cell frequencies in patients.
Article
Immunology
Marisa Flook, Alba Escalera-Balsera, Paulina Rybakowska, Lidia Frejo, Angel Batuecas-Caletrio, Juan C. Amor-Dorado, Andres Soto-Varela, Marta Alarcon-Riquelme, Jose A. Lopez-Escamez
Summary: This study performed mass cytometry and ELISA on peripheral blood samples from Meniere Disease (MD) patients and controls, and found that MD patients can be divided into two clusters based on their cytokine profiles, IgE levels and immune cell populations. The results suggest the existence of an allergic phenotype in some MD patients, which may benefit from personalized IL-4 blockers.
CLINICAL IMMUNOLOGY
(2023)
Article
Biophysics
Armita Salahi, Aditya Rane, Li Xiao, Carlos Honrado, Xudong Li, Li Jin, Nathan S. Swami
Summary: Measurement and modulation of macrophage activation is crucial for addressing chronic back pain and immune rejection. Single-cell methods are needed to quantify the heterogeneity of macrophage activation phenotypes. This study presents a high throughput single-cell impedance cytometry for accurately measuring macrophage activation in a dose and duration dependent manner, without relying on specific signaling pathways.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Jonathan D. Windster, Andrea Sacchetti, Gerben J. Schaaf, Eric M. J. Bindels, Robert M. W. Hofstra, Rene M. H. Wijnen, Cornelius E. J. Sloots, Maria M. Alves
Summary: A selective FACS protocol based on CD56/CD90/CD24 co-expression is developed to enrich and discriminate human ENS cells. Neuronal and glial selective markers are found to be co-expressed by all ENS cells, mainly driven by neuronal fragments. By applying this protocol to single-cell RNA sequencing, high-quality ENS cells are obtained for transcriptome analysis.
Article
Dentistry, Oral Surgery & Medicine
Ofir Ginesin, Yaniv Mayer, Eran Gabay, Daniel Rotenberg, Eli Eliahu Machtei, Benjamin R. R. Coyac, Yotam Bar-On, Hadar Zigdon-Giladi
Summary: This study used flow cytometry to identify, quantify, and characterize leukocyte populations in periodontitis and peri-implantitis. The results showed a high similarity between periodontitis and peri-implantitis in the main immune cell types, with similar proportions of PMNs, T cells, B cells, and macrophages, and low proportions of dendritic and NK cells. Further analysis revealed a higher proportion of CD4-positive cells compared to CD8-positive cells in both diseases.
CLINICAL ORAL INVESTIGATIONS
(2023)
Review
Cell Biology
Zhijun Liu, Francis Ka-Ming Chan
Summary: RIP kinases, including RIPK1 and RIPK3, are key adaptors in inflammatory and cell death signaling pathways. Their activities are tightly regulated by post-translational modifications and environmental factors, which are crucial for proper immune responses and tissue homeostasis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Review
Hematology
Jonathan R. Brestoff
Summary: Contemporary full spectrum or spectral flow cytometry is a recently developed technology that allows for high-dimensional flow cytometric analyses. It gained popularity in research settings and has obtained regulatory approval for in vitro diagnostic use. This review contrasts conventional and spectral flow cytometry and discusses the advantages and preliminary studies of adopting spectral flow cytometry in clinical laboratories.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Article
Immunology
Peicong Ge, Chenglong Liu, Liujia Chan, Yuheng Pang, Hao Li, Qian Zhang, Xun Ye, Jia Wang, Rong Wang, Yan Zhang, Wenjing Wang, Dong Zhang, Jizong Zhao
Summary: This study provides a comprehensive and detailed characterization of the changes in circulating immune cells in patients with intracranial aneurysm (IA). It reveals dysfunction of immunity in IA patients, with dysregulation of CD4(+) T-cell clusters, increased B cells and monocytes, and decreased CD8(+) T cells, DNT cells, and DPT cells. The study also finds enhanced lymphocyte and monocyte immune activation in IA patients, with higher expression of HLA-DR, CXCR3, and CX3CR1.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Kamilah Ryan, Rebecca E. Rose, Drew R. Jones, Peter A. Lopez
Summary: Flow cytometrists have long observed cell-type-specific changes after cell sorting, ranging from minor defects to cell destruction, known as sorter induced cellular stress (SICS). By modifying the fluidic environment of droplet cell sorters, it is possible to rescue the intracellular markers of SICS, which may provide insights into potential electro-physical mechanisms underlying the SICS phenomenon.
Article
Biology
Jana H. Badrani, Allyssa N. Strohm, Yung -An Haung, Taylor A. Doherty
Summary: Innate lymphoid cells (ILCs) are a rare cell population that can be divided into ILC1s, ILC2s, and ILC3s based on gene expression and cytokine production. In models of lung inflammation, ILC2s are activated by alarmins released from the epithelium, leading to increased production and migration of ILC2s within the lung. Flow cytometry is the most reliable method for identifying and characterizing ILCs due to their low abundance and lack of specific surface markers. This protocol utilizes multicolour flow cytometry to identify Lineage-Thy1.2+ ILCs and intracellular cytokine staining to specifically identify ILC2s in the lung.
Article
Biochemistry & Molecular Biology
Andrei S. Rodin, Grigoriy Gogoshin, Seth Hilliard, Lei Wang, Colt Egelston, Russell C. Rockne, Joseph Chao, Peter P. Lee
Summary: This study focuses on predicting clinical responses in cancer immunotherapy by identifying pretreatment biomarkers and analyzing changes in immune networks before and after treatment. By utilizing a novel computational pipeline and systems biology/machine learning techniques, strong signals that may be missed by conventional methods can be detected. Future studies will aim to validate and track immune biomarkers associated with clinical responses identified using this computational pipeline.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Xinxin Chi, Wei Jin, Xue Bai, Xiaohong Zhao, Jing Shao, Jiaqi Li, Qinli Sun, Bing Su, Xiaohu Wang, Xuexian O. Yang, Chen Dong
Summary: The study found that RORα is highly expressed in active UC patients, especially in those non-responsive to anti-TNF treatment. RORα plays a crucial role in promoting IBD development in CD4(+) T cells by regulating T cell infiltration, inhibiting T cell apoptosis, and promoting mTORC1 activation. This indicates that the RORα-mTORC1 axis may be a potential therapeutic target for human patients with IBD.
