Review
Engineering, Biomedical
Zhanping Ren, Anicca D. D. Harriot, Devin B. B. Mair, Michael K. K. Chung, Peter H. U. Lee, Deok-Ho Kim
Summary: The growing interest in bioengineering in-vivo-like 3D functional tissues has led to novel approaches to biomanufacturing and expanded applications. Microgravity, as seen in spaceflight, has potential benefits to tissue engineering but cannot be completely replicated on Earth. Bioengineered microphysiological systems provide an attractive research model due to the practical challenges of conducting research in space. This review summarizes published research on the use of real and simulated microgravity to improve biomanufacturing and model human diseases, discussing platforms, applications, and achievements.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Hyeongjin Lee, Carlos Kengla, Han Su Kim, Ickhee Kim, Jae-Gu Cho, Eric Renteria, Kyungsup Shin, Anthony Atala, James J. Yoo, Sang Jin Lee
Summary: Medical imaging and 3D bioprinting can be used to create patient-specific bone scaffolds with complex shapes and controlled inner architectures. This study investigates the effectiveness of a biomimetic approach to scaffold design and demonstrates that the biomimetic scaffold with a dense external layer improves bone regeneration compared to the control scaffold. Histological examination confirms the superior bone regeneration in the biomimetic scaffolds and suggests that patient-specific anatomy can be accurately translated into a 3D bioprinting strategy, leading to clinically relevant constructs.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Annan Chen, Wanying Wang, Zhengyi Mao, Yunhu He, Shiting Chen, Guo Liu, Jin Su, Pei Feng, Yusheng Shi, Chunze Yan, Jian Lu
Summary: Additive manufacturing (AM) has significant advantages in the fabrication of tissue engineering implants. However, many tissue constructs have anisotropic heterogeneous structures, which pose challenges for conventional AM processes. Multimaterial 3D and 4D bioprinting offer a promising solution for constructing multifunctional implants with heterogeneous structures that can better mimic the host microenvironment.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Alberto Pardo, Syeda Mahwish Bakht, Manuel Gomez-Florit, Ramon Rial, Rosa F. Monteiro, Simao P. B. Teixeira, Pablo Taboada, Rui L. Reis, Rui M. A. Domingues, Manuela E. Gomes
Summary: Combining the concepts of magnetically- and matrix-assisted 3D bioprinting, a new strategy has been developed to fabricate anisotropic high-resolution magnetic composites with remote stimulation capabilities, opening up new possibilities for tissue engineering applications.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Cell Biology
Venkata Suresh Venkataiah, Yoshio Yahata, Akira Kitagawa, Masahiko Inagaki, Yusuke Kakiuchi, Masato Nakano, Shigeto Suzuki, Keisuke Handa, Masahiro Saito
Summary: Bone tissue engineering involves combining MSCs with a biocompatible scaffold to aid in bone regeneration and recovery. While promising results have been seen in clinical trials, regenerating large bone defects remains a challenge.
Review
Engineering, Biomedical
Nikolas Di Caprio, Jason A. Burdick
Summary: Cellular spheroids are being explored as building blocks for tissue engineering and as tissue models. Biomaterials are being used to guide spheroid formation and function, and also facilitate the fabrication of tissue constructs. This review provides an overview of recent studies on the role of engineered biomaterials in supporting spheroids in biomedical applications.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Gopinathan Janarthanan, Jung Hyun Kim, Ingul Kim, Chibum Lee, Eun-Jae Chung, Insup Noh
Summary: This study reports the development of a hydrogel bioink prepared from alginate and hyaluronic acid through multiple crosslinking mechanisms. The resulting gels exhibit exceptional biocompatibility and tunable mechanical properties, making them suitable for 3D bioprinting applications in tissue engineering and regenerative medicine.
Review
Chemistry, Physical
Shuyu Zhang, Guoshi Xu, Juan Wu, Xiao Liu, Yong Fan, Jun Chen, Gordon Wallace, Qi Gu
Summary: In recent years, significant advancements have been made in microphysiological constructs and systems (MPCs and MPSs), providing alternatives to animal models for drug discovery and personalized medicine. However, current approaches mainly focus on the in vitro recapitulation of the human anatomical structure and physiological-biochemical indices at a single or a few simple levels.
Article
Engineering, Biomedical
Min-Chun Tsai, Shih-Yen Wei, Ling Fang, Ying-Chieh Chen
Summary: A patterning technique called viscous fingering has been developed to bioengineer vascularized-like tissues within minutes. By manipulating the physical properties of the base gel, the spreading and growth direction of embedded cells can be successfully controlled and guided.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Trevor Schmitt, Nathan Katz, Vipuil Kishore
Summary: Chronic wounds affect a significant number of people, with current treatment methods mainly focusing on symptomatic relief. Recent research indicates that utilizing 3D bioprinting to generate microfat-laden collagen constructs may be an effective approach in treating chronic wounds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Senthilkumar Muthusamy, Sathya Kannan, Marcus Lee, Vijayavenkataraman Sanjairaj, Wen Feng Lu, Jerry Y. H. Fuh, Gopu Sriram, Tong Cao
Summary: Bioprinting technology has made significant progress over the past decade, particularly in developing larger and more physiologic tissues. By precisely combining biological scaffold materials and cells in three-dimensional space, bioprinting has the potential to assist in locating endothelial cells in specific spatial positions to promote vessel formation in predefined areas.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Engineering, Biomedical
Guang Yang, Bhushan Mahadik, Ji Young Choi, Justine R. Yu, Trevor Mollot, Bin Jiang, Xiaoming He, John P. Fisher
Summary: The study utilized a microfluidic approach to prepare collagen microbeads loaded with human bone marrow derived mesenchymal stem cells for creating engineered bone grafts. The cell-laden microbeads self-assembled into macro-tissues, enabling precise control over cell spatial distribution at the macroscale, offering flexibility for graft volume and architecture. The approach has the potential to create 3D engineered bone grafts with customized vasculature using modular tissue engineering and 3D printing.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Liliang Ouyang, Jonathan P. Wojciechowski, Jiaqing Tang, Yuzhi Guo, Molly M. Stevens
Summary: This study introduces a method to engineer controlled microporosity in 3D bioprinted hydrogels using microgel-templated porogel (MTP) bioinks. By fabricating templated gelatin microgels with different sizes and mixing them with photo-crosslinkable formulations, the researchers achieved consistent printability and the formation of micropores in situ. This approach provides a facile strategy to compensate for the limited resolution of current bioprinting approaches and engineer precise micropores in 3D printed structures.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Austin Worden, Mark J. Uline, Tarek Shazly, Matt Stern, Jay D. Potts
Summary: Developing tissues have complex three-dimensional organizations of cells and extracellular matrix. Researchers have developed a self-assembling cellularized collagen hydrogel model that can rapidly form cell toroids and is sensitive to the composition of the hydrogel. Differences in toroid formation compared to gels with embedded cells were observed, providing new insights into toroid development.
MICROSCOPY AND MICROANALYSIS
(2022)
Review
Biotechnology & Applied Microbiology
Alexandra Cernencu, Andreea Dinu, Izabela C. Stancu, Adriana Lungu, Horia Iovu
Summary: This review focuses on the application of nanoengineered hydrogels in 3D printing of biomimetic constructs, with particular emphasis on the impact of the spatial distribution of reinforcing agents (0D, 1D, 2D). The relationship between relevant length scales and size effects that influence the final properties of hydrogels for regenerative medicine is systematically analyzed, highlighting the use of naturally-derived nanostructured inks.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biochemical Research Methods
Aaydha C. Vinayaka, Mohsen Golabi, Thi Linh Quyen Than, Anders Wolff, Dang D. Bang
Summary: Invasive non-typhoidal salmonellosis is gaining attention as an emerging disease cluster, particularly among immunocompromised and malnourished children. Point-of-care diagnostics hold promise for use in low infrastructure laboratory settings, but face challenges in dealing with the complexity of blood matrices.
Review
Biophysics
Christian Tronstad, Maryam Amini, Dominik R. Bach, orjan G. Martinsen
Summary: Electrodermal activity (EDA) measurement has been transferred from the laboratory to wearable devices, bringing along new applications and opportunities. Challenges remain for the quality of wearable EDA measurement, but ongoing developments in instrumentation, material technology, and data science may shorten the quality gap between wearable EDA and standardized recordings in the laboratory.
PHYSIOLOGICAL MEASUREMENT
(2022)
Article
Multidisciplinary Sciences
Arianna Toppi, Martin Dufva
Summary: A new method for fabricating microdroplet array (MDA) substrates independently from cleanroom processes was developed. A low-cost in-house built system for photopatterning a negative resist was assembled to produce stable and high-quality MDAs. The feasibility for carrying out digital assays was demonstrated.
Article
Immunology
Than Linh Quyen, Aaydha Chidambara Vinayaka, Mohsen Golabi, Huynh Van Ngoc, Dang Duong Bang, Anders Wolff
Summary: This study developed a new LAMP assay that can eliminate carryover contamination and rapidly detect SARS-CoV-2. The assay can detect the virus as low as 2 copies/μl within 45 minutes and eliminate a significant amount of contaminants. Analysis of clinical samples showed high agreement with rRT-PCR.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Huynh Van Ngoc, Than Linh Quyen, Aaydha Chidambara Vinayaka, Dang Duong Bang, Anders Wolff
Summary: This study developed a fluorescence-based portable detection system for rapid and quantitative detection of the SARS-CoV-2 virus. The system, built on the Arduino platform, demonstrated real-time detection capability and showed excellent agreement with a commercial thermocycler. It has the potential to greatly enhance the practical applicability for on-site screening of the SARS-CoV-2 virus.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shashank Vasudevan, Andrea Dotti, Janko Kajtez, Alberto Martinez-Serrano, Carsten Gundlach, Sandrina Campos Macas, Karin Lauschke, Anne-Marie Vinngaard, Silvia Garcia Lopez, Marta Pereira, Arto Heiskanen, Stephan S. Keller, Jenny Emneus
Summary: This study introduces a novel approach to generate Omni-LOEFs using laser ablation and demonstrates that dopamine detection current recorded from neurons differentiated on Omni-LOEFs upon light-induced stimulation is significantly higher compared to other materials.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Maria Niora, Mathilde Hauge Lerche, Martin Dufva, Kirstine Berg-Sorensen
Summary: Fluorescent nanodiamonds (FNDs) with negative nitrogen-vacancy (NV-) defect centers are ideal probes for biosensing applications and can potentially serve as biomarkers for cell differentiation. The uptake of FNDs by THP-1 monocytes and monocyte-derived M0-macrophages is studied, revealing differing FND-cell interactions and time-dependent uptake. FND uptake allows for distinction between cell subtypes based on phagocytic capacity, and FNDs demonstrate effective cell labeling and have potential for sensing biological processes involving cell differentiation.
Article
Biotechnology & Applied Microbiology
Asli Aybike Dogan, Martin Dufva
Summary: This study introduces a customized hanging insert for cultivating Caco-2 cells on hydrogel membranes under flow conditions. The device consists of an apical channel-like chamber for cell culture with shear force and a basolateral chamber that is mixed when rocked. The experiment investigates the impact of seeding density and flow on the growth of the cells. The results show that low seeding density and flow promote the formation of three-dimensional structures and improve barrier function.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Multidisciplinary Sciences
Martin Dufva
Summary: Out of 2828 screened articles, only 146 contained controls and quantified data for flow cell culture. Analysis of these articles showed that many biomarkers in all cell types were unaffected by flow, while only specific biomarkers responded strongly to flow.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Janko Kajtez, Carmen Radeke, Johan Ulrik Lind, Jenny Emneus
Summary: In the past decade, embedded 3D printing has emerged as a powerful tool for biofabrication of soft tissue constructs. However, the available granular gel support media have lacked the cell-adhesive and cell-instructive functions. To address this, a methodology for the generation of self-healing annealable particle-extracellular matrix (SHAPE) composites has been developed, which allows for both high-fidelity printing and adjustable biofunctional extracellular environment. This study demonstrates the precise biofabrication of human neural constructs using SHAPE composites.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Chemistry, Analytical
Aaydha Chidambara Vinayaka, Van Ngoc Huynh, Than Linh Quyen, Trieu Nguyen, Mohsen Golabi, Mogens Madsen, Dang Duong Bang, Anders Wolff
Summary: Accurate and rapid detection of pathogens in animal-origin foods is crucial for the One Health Action Plan of the EU. We developed a LAMP-based point-of-care device called VETPOD for detecting Salmonella spp. The VETPOD uses a unique cartridge with pyramid-shaped optical structures for light detection, achieving comparable results to the ISO 6579-1 reference method with high sensitivity (96.51%) and specificity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Trieu Nguyen, Aaydha Chidambara Vinayaka, Van Ngoc Huynh, Quyen Than Linh, Sune Zoega Andreasen, Mohsen Golabi, Dang Duong Bang, Jens Kjolseth Moller, Anders Wolff
Summary: This study presents a comprehensive work on the fabrication and clinical validation of a point of care (PoC) device for rapid and onsite detection of SARS-CoV-2. The device, named PATHPOD, can perform the detection of 10 different samples and two controls in less than 50 min, using a real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) reaction on a polymer cartridge. The test has comparable sensitivity and specificity to the current golden standard RT-PCR.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Proceedings Paper
Engineering, Electrical & Electronic
Trieu Nguyen, Aaydha Chidambara Vinayaka, Van Ngoc Huynh, Quyen Than Linh, Sune Zoega Andreasen, Mohsen Golabi, Dang Duong Bang, Jens Kjolseth Moller, Anders Wolff
Summary: Sensitive and rapid on-site detection of SARS-CoV-2 using the PATHPOD device, which employs real-time reverse-transcription loop-mediated isothermal amplification (RT-rLAMP) reaction on a polymer cartridge, has been achieved. The PATHPOD PoC system, consisting of a standalone device and a cartridge, can identify 10 distinct samples and 2 controls in less than 50 minutes. This work presents the fabrication and clinical validation of the PATHPOD PoC system for the first time.
2023 IEEE 36TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, MEMS
(2023)
Proceedings Paper
Computer Science, Artificial Intelligence
Abbas Tariverdi, Kim Mathiassen, Vegard Soyseth, Havard Kalvoy, Ole J. Elle, Jim Torresen, Orjan G. Martinsen, Mats Erling Hovin
Summary: This paper establishes a physics-based simulation framework for steering a magnetically actuated guidewire based on the linear elasticity and dipoles theories. The framework employs a simplified integration scheme based on the finite-volume method to model the guidewire using the linear elasticity theory and forces resulting from the interference of magnetic fields. Simulations illustrate the approach performance on a soft rod, demonstrating its potential for constructing rapid models for continuum manipulators in practice.
2022 8TH INTERNATIONAL CONFERENCE ON CONTROL, DECISION AND INFORMATION TECHNOLOGIES (CODIT'22)
(2022)
