Article
Plant Sciences
Mazin Mahjoob Mohamed Mahjoob, Yasir Serag Alnor Gorafi, Nasrein Mohamed Kamal, Yuji Yamasaki, Izzat Sidahmed Ali Tahir, Yoshihiro Matsuoka, Hisashi Tsujimoto
Summary: Aegilops tauschii, the D-genome donor of bread wheat, is a genetic resource with a large diversity of morpho-physiological traits and adaptations to a wide ecological range. Through genome-wide association studies, markers or genes associated with morpho-physiological traits were identified, revealing differences in genetic diversity between different lineages, which are valuable for wheat breeding.
Article
Biotechnology & Applied Microbiology
Siyu Zhang, Pei Du, Xueying Lu, Jiaxin Fang, Jiaqi Wang, Xuejun Chen, Jianyong Chen, Hao Wu, Yang Yang, Hisashi Tsujimoto, Chenggen Chu, Zengjun Qi
Summary: This study investigates the impact of chromosome variations on wheat evolution using synthetic hexaploid wheat (SHW). Analysis of SHW lines using chromosome FISH and SNP marker clustering reveals non-random chromosome variations, indicating the occurrence of similar variations in the early stages of wheat polyploidization and their important role in wheat evolution.
Article
Biotechnology & Applied Microbiology
Rizky Pasthika Kirana, Kumar Gaurav, Sanu Arora, Gerlinde Wiesenberger, Maria Doppler, Sebastian Michel, Simone Zimmerl, Magdalena Matic, Chinedu E. Eze, Mukesh Kumar, Ajla Topuz, Marc Lemmens, Rainer Schuhmacher, Gerhard Adam, Brande B. H. Wulff, Hermann Buerstmayr, Barbara Steiner
Summary: This study investigated the genetic basis of resistance against Fusarium head blight and the mycotoxin deoxynivalenol in Aegilops tauschii, the diploid progenitor of the wheat D subgenome. By analyzing a panel of Ae. tauschii accessions, a uridine diphosphate (UDP)-glucosyltransferase (UGT) gene on chromosome 5D was identified as the causal variant for susceptibility to the mycotoxin. This UGT gene was also found to be involved in DON detoxification in hexaploid wheat. The findings highlight the conservation of this resistance mechanism in wheat and barley.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Ali A. Naz, Annemarie Bungartz, Albrecht Serfling, Mohammad Kamruzzaman, Michael Schneider, Brande B. H. Wulff, Klaus Pillen, Agim Ballvora, Erich-Christian Oerke, Frank Ordon, Jens Leon
Summary: This study investigated the evolutionary and functional divergence of the leaf rust resistance gene Lr21 in diploid and hexaploid wheats through population genetics and high-resolution comparative genomics. The research identified original Lr21 alleles in Aegilops tauschii and discovered a new Lr21-tbk allele in hexaploid wheat cv. Tobak. In addition to seedling resistance, the Lr21 gene was found to confer adult plant field resistance in wheat, with resistance correlated with developmentally dependent variation in Lr21 expression.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
Kumar Gaurav, Sanu Arora, Paula Silva, Javier Sanchez-Martin, Richard Horsnell, Liangliang Gao, Gurcharn S. Brar, Victoria Widrig, W. John Raupp, Narinder Singh, Shuangye Wu, Sandip M. Kale, Catherine Chinoy, Paul Nicholson, Jesus Quiroz-Chavez, James Simmonds, Sadiye Hayta, Mark A. Smedley, Wendy Harwood, Suzannah Pearce, David Gilbert, Ngonidzashe Kangara, Catherine Gardener, Macarena Forner-Martinez, Jiaqian Liu, Guotai Yu, Scott A. Boden, Attilio Pascucci, Sreya Ghosh, Amber N. Hafeez, Tom O'Hara, Joshua Waites, Jitender Cheema, Burkhard Steuernagel, Mehran Patpour, Annemarie Fejer Justesen, Shuyu Liu, Jackie C. Rudd, Raz Avni, Amir Sharon, Barbara Steiner, Rizky Pasthika Kirana, Hermann Buerstmayr, Ali A. Mehrabi, Firuza Y. Nasyrova, Noam Chayut, Oadi Matny, Brian J. Steffenson, Nitika Sandhu, Parveen Chhuneja, Evans Lagudah, Ahmed F. Elkot, Simon Tyrrell, Xingdong Bian, Robert P. Davey, Martin Simonsen, Leif Schauser, Vijay K. Tiwari, H. Randy Kutcher, Pierre Hucl, Aili Li, Deng-Cai Liu, Long Mao, Steven Xu, Gina Brown-Guedira, Justin Faris, Jan Dvorak, Ming-Cheng Luo, Ksenia Krasileva, Thomas Lux, Susanne Artmeier, Klaus F. X. Mayer, Cristobal Uauy, Martin Mascher, Alison R. Bentley, Beat Keller, Jesse Poland, Brande B. H. Wulff
Summary: The study sequenced 242 accessions of Aegilops tauschii and identified a rare lineage contributing to the wheat D subgenome. Through association mapping, genomic regions with candidate genes for disease and pest resistance were identified and successfully transferred into wheat. Exploiting the genomic diversity of the wild progenitor allows for rapid trait discovery and functional genetic validation.
NATURE BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ryan Joynson, Gemma Molero, Benedict Coombes, Laura-Jayne Gardiner, Carolina Rivera-Amado, Francisco J. Pinera-Chavez, John R. Evans, Robert T. Furbank, Matthew P. Reynolds, Anthony Hall
Summary: This study surveyed a diverse set of wheat germplasm and demonstrated the value of genomic methods in uncovering hidden genetic variation. The strategic integration of exotic material alleviated the genetic bottleneck in wheat, increasing SNP rate significantly. By utilizing association analysis, candidate genes for traits related to photosynthetic capacity were identified, showing how genomic methods can assist breeding efforts and enhance our understanding of complex traits in wheat.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Agriculture, Multidisciplinary
Haiyan Yu, Xiaotong Guo, Hailan Cui, Jingchao Chen, Xiangju Li
Summary: The tolerance of Triticum aestivum to mesosulfuron-methyl is higher than that of A. tauschii. The absorption and metabolism of mesosulfuron-methyl are different between the two species. The cytochrome P450 monooxygenase inhibitor, malathion, increases the sensitivity of T. aestivum to mesosulfuron-methyl.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agronomy
Maria Ciudad-Mulero, Maria Cruz Matallana-Gonzalez, Maria Jesus Callejo, Jose M. Carrillo, Patricia Morales, Virginia Fernandez-Ruiz
Summary: This study evaluated the mineral composition of different wheat varieties, finding higher concentrations of minerals in bran and whole grain flour, especially potassium and magnesium. The results suggest that some samples meet the conditions for approved health claims, potentially supporting the classification of wheat flour as functional foods.
Article
Plant Sciences
Kari Johnson, Uyen Cao Chu, Geny Anthony, Emily Wu, Ping Che, Todd J. Jones
Summary: Two wheat transformation methods using morphogenic regulator genes, with or without gene excision, have been developed. These methods achieve independent transformation efficiencies of up to 58% and 75%, respectively, and significantly reduce tissue culture duration. The procedures are simplified, less labor-intensive, higher-throughput, and cost-effective.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biodiversity Conservation
Mazin Mahjoob Mohamed Mahjoob, Tai-Shen Chen, Yasir Serag Alnor Gorafi, Yuji Yamasaki, Nasrein Mohamed Kamal, Mostafa Abdelrahman, Hiroyoshi Iwata, Yoshihiro Matsuoka, Izzat Sidahmed Ali Tahir, Hisashi Tsujimoto
Summary: The study aimed to clarify the phylogeny of Ae. tauschii and identify traits that differentiate the three lineages and subspecies, revealing wide morpho-physiological variation but no key traits to discriminate between them. The genetic and morpho-physiological diversity in 293 accessions of Ae. tauschii was analyzed, resulting in allocation of previously unknown accessions to different lineages and subspecies based on SNP analyses.
Article
Agronomy
Haiyan Yu, Juan Yang, Hailan Cui, Adeel Abbas, Shouhui Wei, Xiangju Li
Summary: Aegilops tauschii, a noxious grass weed affecting wheat quality and yield, was found in wheat fields across various provinces in China. Genetic diversity was moderately high with no clear geographic patterns. Structure analysis divided the populations into three groups, with majority of variation occurring within population groups.
Article
Biotechnology & Applied Microbiology
Di-li Lai, Jun Yan, Yu Fan, Yao Li, Jing-jun Ruan, Jun-zhen Wang, Yue Fan, Xiao-bin Cheng, Jian-ping Cheng
Summary: This study conducted a comprehensive bioinformatics analysis of Hsp70 genes in bread wheat, wild emmer wheat, and Aegilops tauschii, identifying differences in gene classification, evolution, and expression between the three species. It was found that Hsp70 genes had undergone purification selection and were highly expressed under stress, providing a basis for further research on their evolution and molecular mechanisms in response to stress. Overall, the study shed light on the importance of Hsp70 in plant development and stress response.
Article
Genetics & Heredity
Silvia Barcellos Rosa, Gavin Humphreys, Linda Langille, Harvey Voldeng, Maria Antonia Henriquez, Andrew James Burt, Harpinder Singh Randhawa, Tom Fetch, Colin W. Hiebert, Barbara Blackwell, Taye Zegeye, Allan Cummiskey, Eric Fortier, Pedro Luiz Scheeren, Camila Turra, Brent McCallum
Summary: In the era of climate instability, genetic diversity from Brazilian germplasm can contribute to sustainable and improved wheat production in Canada through increased disease resistance and genetic variability. Evaluating over 100 Brazilian wheat cultivars showed some with good adaptability and superior agronomic performance compared to Canadian checks. Brazilian cultivars exhibited resistance to leaf rust, stem rust, and powdery mildew, as well as Fusarium head blight. They also possessed valuable semi-dwarf genes and were genetically distinct from Canadian wheat.
FRONTIERS IN GENETICS
(2023)
Article
Plant Sciences
Manel Othmeni, Surbhi Grewal, Jack Walker, Cai-yun Yang, Ian P. King, Julie King
Summary: Aegilops tauschii, the D-genome donor of hexaploid wheat, provides genetic variation for the improvement of tetraploid wheat. The introgression of Ae. tauschii into durum wheat requires cytogenetic manipulation and specific markers for screening, but it is challenging.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Gazaldeep Kaur, Vishnu Shukla, Varsha Meena, Anil Kumar, Deepshikha Tyagi, Jagtar Singh, Pramod Kaitheri Kandoth, Shrikant Mantri, Hatem Rouached, Ajay Kumar Pandey
Summary: Iron (Fe) and phosphorus (P) are essential mineral nutrients for plant growth, and their molecular interactions in crops remain unclear. This study analyzed the physiological and molecular responses of hexaploid wheat to individual and combined Fe and P deficiencies, revealing significant gene expression changes and metabolite accumulation, providing insights for developing strategies to enhance crop resilience in limited nutrient conditions.
Article
Biotechnology & Applied Microbiology
M. Asyraf Md Hatta, Sanu Arora, Sreya Ghosh, Oadi Matny, Mark A. Smedley, Guotai Yu, Soma Chakraborty, Dhara Bhatt, Xiaodi Xia, Burkhard Steuernagel, Terese Richardson, Rohit Mago, Evans S. Lagudah, Nicola J. Patron, Michael Ayliffe, Matthew N. Rouse, Wendy A. Harwood, Sambasivam Periyannan, Brian J. Steffenson, Brande B. H. Wulff
Summary: This study evaluated the functionality of cloned wheat Sr genes in conferring effective stem rust resistance in barley, showing that the transgenic barley plants remained susceptible to barley leaf rust pathogen while showing resistance specifically against Pgt. These transgenic plants did not display significant adverse agronomic effects in the absence of disease.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Ming Luo, Liqiong Xie, Soma Chakraborty, Aihua Wang, Oadi Matny, Michelle Jugovich, James A. Kolmer, Terese Richardson, Dhara Bhatt, Mohammad Hoque, Mehran Patpour, Chris Sorensen, Diana Ortiz, Peter Dodds, Burkhard Steuernagel, Brande B. H. Wulff, Narayana M. Upadhyaya, Rohit Mago, Sambasivam Periyannan, Evans Lagudah, Roger Freedman, T. Lynne Reuber, Brian J. Steffenson, Michael Ayliffe
Summary: Through the introduction of a transgene cassette containing five resistance genes into bread wheat, researchers have successfully generated wheat lines with highly resistant to aggressive and highly virulent Pgt isolates from around the world. This approach simplifies breeding efforts and enhances resistance levels, although strategic deployment of gene stacks may be needed to maintain effectiveness against new Pgt isolates.
NATURE BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Tim Hewitt, Marion C. Muller, Istvan Molnar, Martin Mascher, Katerina Holusova, Hana Simkova, Lukas Kunz, Jianping Zhang, Jianbo Li, Dhara Bhatt, Raghvendra Sharma, Seraina Schudel, Guotai Yu, Burkhard Steuernagel, Sambasivam Periyannan, Brande Wulff, Mick Ayliffe, Robert McIntosh, Beat Keller, Evans Lagudah, Peng Zhang
Summary: The discovery of the first powdery mildew resistance gene Pm1a in wheat revealed its location in a distal region of chromosome 7AL suppressing genetic recombination. Through transformation, genome sequencing, and expression studies, the resistance mechanism of Pm1a was successfully identified and characterized.
Article
Biotechnology & Applied Microbiology
Chunhong Chen, Matthias Jost, Bethany Clark, Matthew Martin, Oadi Matny, Brian J. Steffenson, Jerome D. Franckowiak, Martin Mascher, Davinder Singh, Dragan Perovic, Terese Richardson, Sambasivam Periyannan, Evans S. Lagudah, Robert F. Park, Peter M. Dracatos
Summary: Leaf rust, caused by Puccinia hordei, is a severe fungal disease globally impacting barley production. Cloning of the Rph15 resistance gene from a wild barley progenitor and development of a KASP marker provide a new avenue for sustained leaf rust control. The resistance mediated by Rph15 and previously thought to be allelic with Rph16 is actually the same, allowing for efficient gene deployment in combating leaf rust.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Letter
Biochemistry & Molecular Biology
Timothy Hewitt, Jianping Zhang, Li Huang, Narayana Upadhyaya, Jianbo Li, Robert Park, Sami Hoxha, Robert McIntosh, Evans Lagudah, Peng Zhang
Article
Entomology
A. Sabraoui, L. Emebiri, W. Tadesse, F. C. Ogbonnaya, K. El Fakhouri, M. El Bouhssini
Summary: Synthetic hexaploid wheat lines have shown resistance to both Moroccan Hessian fly biotype and Syrian Sunn pest. Ongoing research is focusing on genetic characterization and identification of molecular markers for marker assisted selection using these sources of resistance.
JOURNAL OF APPLIED ENTOMOLOGY
(2021)
Article
Multidisciplinary Sciences
Jianping Zhang, Timothy C. Hewitt, Willem H. P. Boshoff, Ian Dundas, Narayana Upadhyaya, Jianbo Li, Mehran Patpour, Sutha Chandramohan, Zacharias A. Pretorius, Mogens Hovmoller, Wendelin Schnippenkoetter, Robert F. Park, Rohit Mago, Sambasivam Periyannan, Dhara Bhatt, Sami Hoxha, Soma Chakraborty, Ming Luo, Peter Dodds, Burkhard Steuernagel, Brande B. H. Wulff, Michael Ayliffe, Robert A. McIntosh, Peng Zhang, Evans S. Lagudah
Summary: Sr26 and Sr61 are effective stem rust resistance genes encoding two unrelated proteins, isolated from wheat with introgressions from tall wheat grass. They are present on a small non-recombinogenic segment, allowing for gene stacking and potentially enhancing resistance durability.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Kumar Gaurav, Sanu Arora, Paula Silva, Javier Sanchez-Martin, Richard Horsnell, Liangliang Gao, Gurcharn S. Brar, Victoria Widrig, W. John Raupp, Narinder Singh, Shuangye Wu, Sandip M. Kale, Catherine Chinoy, Paul Nicholson, Jesus Quiroz-Chavez, James Simmonds, Sadiye Hayta, Mark A. Smedley, Wendy Harwood, Suzannah Pearce, David Gilbert, Ngonidzashe Kangara, Catherine Gardener, Macarena Forner-Martinez, Jiaqian Liu, Guotai Yu, Scott A. Boden, Attilio Pascucci, Sreya Ghosh, Amber N. Hafeez, Tom O'Hara, Joshua Waites, Jitender Cheema, Burkhard Steuernagel, Mehran Patpour, Annemarie Fejer Justesen, Shuyu Liu, Jackie C. Rudd, Raz Avni, Amir Sharon, Barbara Steiner, Rizky Pasthika Kirana, Hermann Buerstmayr, Ali A. Mehrabi, Firuza Y. Nasyrova, Noam Chayut, Oadi Matny, Brian J. Steffenson, Nitika Sandhu, Parveen Chhuneja, Evans Lagudah, Ahmed F. Elkot, Simon Tyrrell, Xingdong Bian, Robert P. Davey, Martin Simonsen, Leif Schauser, Vijay K. Tiwari, H. Randy Kutcher, Pierre Hucl, Aili Li, Deng-Cai Liu, Long Mao, Steven Xu, Gina Brown-Guedira, Justin Faris, Jan Dvorak, Ming-Cheng Luo, Ksenia Krasileva, Thomas Lux, Susanne Artmeier, Klaus F. X. Mayer, Cristobal Uauy, Martin Mascher, Alison R. Bentley, Beat Keller, Jesse Poland, Brande B. H. Wulff
Summary: The study sequenced 242 accessions of Aegilops tauschii and identified a rare lineage contributing to the wheat D subgenome. Through association mapping, genomic regions with candidate genes for disease and pest resistance were identified and successfully transferred into wheat. Exploiting the genomic diversity of the wild progenitor allows for rapid trait discovery and functional genetic validation.
NATURE BIOTECHNOLOGY
(2022)
Article
Agronomy
Rohit Mago, Chunhong Chen, Xiaodi Xia, Alex Whan, Kerrie Forrest, Bhoja R. Basnet, Geetha Perera, Sutha Chandramohan, Mandeep Randhawa, Matthew Hayden, Urmil Bansal, Julio Huerta-Espino, Ravi P. Singh, Harbans Bariana, Evans Lagudah
Summary: The adult plant stem rust resistance gene Sr63 was identified in durum wheat Glossy Huguenot and molecular markers were developed for its detection. QTL for stem rust resistance were found on chromosomes 1B and 2A, and combinations of these QTL were effective in reducing stem rust severity.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Agronomy
Naveenkumar Athiyannan, Yunming Long, Houyang Kang, Sutha Chandramohan, Dhara Bhatt, Qijun Zhang, Daryl L. Klindworth, Matthew N. Rouse, Timothy L. Friesen, Robert McIntosh, Peng Zhang, Kerrie Forrest, Mathew Hayden, Mehran Patpour, Mogens S. Hovmoller, Lee T. Hickey, Michael Ayliffe, Xiwen Cai, Evans S. Lagudah, Sambasivam Periyannan, Steven S. Xu
Summary: This study identified resistance genes for wheat stem rust in two accessions of Aegilops tauschii, the diploid progenitor of wheat. These genes are sequence variants of Sr46 and show resistance to multiple races of the stem rust pathogen. The identification of these genes provides valuable resources for improving stem resistance in wheat.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Peipei Zhang, Caixia Lan, Ravi P. Singh, Julio Huerta-Espino, Zaifeng Li, Evans Lagudah, Sridhar Bhavani
Summary: This study identified several genes and loci conferring resistance to leaf rust and stripe rust through genetic and QTL mapping research, including a novel pleiotropic gene conferring resistance to both leaf rust and stripe rust in an Afghan landrace. The discovery of these genes and loci is significant for breeding efforts to develop durable resistance to multiple rust diseases.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Naveenkumar Athiyannan, Peng Zhang, Robert McIntosh, Soma Chakraborty, Timothy Hewitt, Dhara Bhatt, Kerrie Forrest, Narayana Upadhyaya, Burkard Steuernagel, Sanu Arora, Julio Huerta, Mathew Hayden, Brande B. H. Wulff, Michael Ayliffe, Lee T. Hickey, Evans Lagudah, Sambasivam Periyannan
Summary: The stripe rust resistance gene YrAet672 from Aegilopstauschii accession CPI110672 was found to confer effective resistance against multiple pathotypes of the wheat stripe rust pathogen Pucciniastriiformis Westend. f. sp. tritici Erikss. (Pst). The YrAet672 gene was mapped to chromosome 4D and encoded a nucleotide-binding and leucine-rich repeat protein. Furthermore, it was discovered that YrAet672 is the same as two other Pst resistance genes described in Ae.tauschii.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Demei Liu, Chan Yuan, Ravi P. P. Singh, Mandeep S. S. Randhawa, Sridhar Bhavani, Uttam Kumar, Julio Huerta-Espino, Evans Lagudah, Caixia Lan
Summary: Developing wheat varieties with durable resistance is a key objective for breeding programs worldwide. This study identified genetic basis and resistance loci for stripe rust (YR) and leaf rust (LR) in wheat, and developed molecular markers for accelerated breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Ricky J. Milne, Katherine E. Dibley, Jayakumar Bose, Anthony R. Ashton, Peter R. Ryan, Stephen D. Tyerman, Evans S. Lagudah
Summary: A wheat gene that provides partial resistance to multiple fungal diseases has been found to be associated with increased ion transport, which could contribute to disease resistance.
Article
Agronomy
Motonori Tomita, Keiko Nakatsuka, Natsuko Morita, Evans Lagudah, Rudi Appels
Summary: In this study, a NBS-LRR-type disease resistance gene-like cDNA induced by salicylic acid was cloned from rye. Various techniques, including PCR, TA-cloning, and cDNA library screening, were used to analyze the gene sequence in Petkus rye.