Article
Biochemistry & Molecular Biology
Manuel Mata, Santiago Peydro, Jose Javier Martin de Llano, Maria Sancho-Tello, Carmen Carda
Summary: Periodontitis is an inflammatory disease that can lead to tooth loss. This study examined the role of surface microtopography in the differentiation of human dental pulp stem cells into cementoid-like secreting cells. The results showed that the cells cultured on dentin surfaces exhibited an odontoblastic phenotype, while cells cultured on cementum surfaces generated a well-organized tissue consisting of secretory cells and dense fibrous connective tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Kunihiro Kajiura, Naoki Umemura, Emika Ohkoshi, Takahisa Ohta, Nobuo Kondoh, Satoshi Kawano
Summary: The study demonstrates that shikonin can induce odontoblastic differentiation of dental pulp stem cells through the involvement of the AKT-mTOR signaling pathway and CD44. While CD44 expression is crucial for odontoblastic differentiation of dental pulp stem cells, the relationship between shikonin-induced expression of DSPP and the AKT-mTOR signaling pathway and CD44 expression remains to be fully elucidated.
CONNECTIVE TISSUE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Muhammad Chair Effendi, Ahmad Taufiq, Boy Muchlis Bachtiar, Endang Winiati Bachtiar, Ellyza Herda
Summary: The study revealed that DPSCs exposed to NMT showed enhanced proliferation and differentiation, demonstrating dentin formation and extracellular matrix formation.
Article
Biochemistry & Molecular Biology
Cristina- Bucchi, Ella Ohlsson, Josep Maria de Anta, Melanie Woelflick, Kerstin Galler, Maria Cristina Manzanares-Cespedes, Matthias Widbiller
Summary: Pluripotent stem cells derived from the amnion show the ability to adhere to dentin and mineralize in vitro, but exhibit unfavorable proliferation behavior and fail to undergo odontoblastic transition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Vellore Kannan Gopinath, Sheela Soumya, V. Yogeshwar Chakrapani, T. S. Sampath Kumar
Summary: This study developed electrospun nanofibrous membranes blending PCL with HA, with PCL10%HA membrane showing the most favorable effects on cell attachment, upregulation of gene expression, and Ca/P ratio compared to PCL and PCL15%HA membranes in inducing differentiation of IDPSCs into odontoblasts.
DENTAL MATERIALS JOURNAL
(2021)
Article
Engineering, Biomedical
Sirui Liu, Jingjing Sun, Shuai Yuan, Yanyu Yang, Yuping Gong, Ying Wang, Runying Guo, Xue Zhang, Yiming Liu, Hongyan Mi, Meiyue Wang, Mengzhe Liu, Rui Li
Summary: The study found that TDM can promote the odontogenic differentiation of human dental pulp stem cells, decrease the expression of GSK3 beta, and activate the Wnt/beta-catenin signaling pathway, thereby promoting the generation of new dentin.
BIOACTIVE MATERIALS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Chatvadee Kornsuthisopon, Jeeranan Manokawinchoke, Opor Sonpoung, Thanaphum Osathanon, Damrong Damrongsri
Summary: IL15 was found to promote osteogenic differentiation in hDPs and participate in Jagged1-induced mineralization. The use of a JAK inhibitor can attenuate IL15-induced mineral deposition and inhibit the effect of Jagged1 on hDP mineral deposition.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Jie Chen, Huaxing Xu, Kun Xia, Shuhua Cheng, Qi Zhang
Summary: In this study, it was found that RvE1 promotes pulp inflammation resolution and dentin regeneration, and positively influences the proliferation, chemotaxis, and differentiation of LPS-stimulated hDPSCs. The effects of RvE1 on DPSCs were mediated at least partially through AKT, ERK, and rS6-associated signaling in the inflammatory microenvironment. RvE1 shows promising application potential in regenerative endodontics.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Physiology
Lisha Zhou, Shuya Zhao, Xianghui Xing
Summary: This review summarizes six major signaling pathways involved in the odontogenic differentiation of dental pulp stem cells (DPSCs) and highlights the influence of various factors on DPSCs through these pathways. By studying the interactions between these signaling pathways, we can gain a better understanding of the mechanisms underlying the regeneration of the pulp-dentin complex.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Chao Wang, Yuejun Wang, Haiyan Wang, Haoqing Yang, Yangyang Cao, DengSheng Xia, Yu Cao, Zhipeng Fan
Summary: The study found that over-expression of SFRP2 significantly enhanced dentinogenesis in DPSCs, promoting dentin regeneration, which may have potential clinical applications.
Article
Cell Biology
Meiling Wu, Xuemei Liu, Zihan Li, Xiaoyao Huang, Hao Guo, Xiaohe Guo, Xiaoxue Yang, Bei Li, Kun Xuan, Yan Jin
Summary: The study demonstrated that SHED aggregate-derived exosomes significantly improved pulp tissue regeneration and angiogenesis by regulating miR-26a and the TGF-beta/SMAD2/3 signaling pathway to enhance vascular formation in SHED and HUVECs.
CELL PROLIFERATION
(2021)
Review
Dentistry, Oral Surgery & Medicine
Minyan Yuan, Xueqing Zheng, Yifan Xue, Zhenru He, Guangtai Song, Yaling Song
Summary: The study aims to investigate a Chinese family with dentin dysplasia type II (DD-II) and summarize mutation hotspots, clinical manifestations, and disease management strategies. A novel pathogenic mutation (c.2035delA) in the DPP region of DSPP was identified, which was cosegregated in the family. The study revealed new clinical features of DD-II and identified a mutation hotspot in the DSPP gene.
Article
Cell Biology
Suman Kanji, Ripon Sarkar, Asmita Pramanik, Sudhir Kshirsagar, Carl J. Greene, Hiranmoy Das
Summary: Dental pulp-derived stem cells (DPSCs) limit osteoclast (OC) differentiation by reducing the expression of key markers, mediating M2 polarization of RAW 264.7 cells. Furthermore, DPSCs up-regulate the OC inhibitory factor osteoprotegerin (OPG) in RAW 264.7 cells and constitutively secrete OPG to limit OC differentiation. Moreover, the inhibition of OC differentiation by DPSCs is similar to the inhibition mediated by OPG through downstream activation of the PI3K-AKT signaling pathway.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Ruoman Xu, Ziyu Zhou, Danle Lin, Lingling Yuan, Siyu Wang, Meng Xu, Yanan Chen, Xiaoli Hu
Summary: Chondroitin sulfate (CS) immobilized in collagen I (Col I) hydrogels was found to induce odontogenic differentiation of dental pulp stem cells (DPSCs) and promote high-quality reparative dentin formation. CS improved DPSCs proliferation and enhanced mineralization activities. In vivo experiments demonstrated that CS-0.1 hydrogel induced higher quality reparative dentin formation compared to mineral trioxide aggregate. Therefore, CS-Col I hydrogel has the potential for high-quality reparative dentin formation in direct pulp capping.
JOURNAL OF ENDODONTICS
(2023)
Article
Biochemistry & Molecular Biology
Lina Yu, Liting Zeng, Zeyu Zhang, Guanxiong Zhu, Zidan Xu, Junyi Xia, Jinlong Weng, Jiang Li, Janak Lal Pathak
Summary: This study found that cannabidiol enhances the proliferation, migration, and osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). CBD also exhibits anti-inflammatory, chemotactic, anti-microbial, and tissue regenerative potentials. These findings suggest the potential application of CBD in dental pulp and bone regeneration based on DPSCs.
