Article
Agronomy
A. Jeevalatha, C. N. Biju, R. Suseela Bhai
Summary: Foot rot caused by Phytophthora is a major disease of black pepper. Accurate and timely diagnosis of the disease is crucial for its management. Recombinase polymerase amplification (RPA) assay has the advantages of minimal assay time and isothermal conditions, making it a potential tool for detection and differentiation of Phytophthora species infecting black pepper.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Mingzhu Li, Qian Guo, Mengyi Liang, Qing Zhao, Tao Lin, Han Gao, Ayaka Hieno, Koji Kageyama, Xin Zhang, Langjun Cui, Yaping Yan, Yi Qiang
Summary: China has the largest kiwifruit production area in the world, and this study focused on investigating the pathogens associated with root diseases of kiwi trees. Three Phytophthora species were studied, and P. cactorum was found to be the most widely distributed. Soil temperature and pH were found to significantly affect the abundance of P. cactorum and P. cinnamomi. Moreover, a rapid and accurate detection system for P. cactorum was developed using loop-mediated isothermal amplification.
Article
Plant Sciences
Zhiyuan Yin, Nan Wang, Weiwei Duan, Lei Pi, Danyu Shen, Daolong Dou
Summary: The CBM1 family is predominantly found in fungi and oomycetes, playing crucial roles in plant-microbe interactions. PcCBP3, identified in Phytophthora capsici, is an apoplastic effector that induces cell death in plants, suggesting its importance in the virulence of the pathogen.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Multidisciplinary Sciences
Mincai Nie, Yuancheng Zhou, Fengqin Li, Huidan Deng, Mengxi Zhao, Yao Huang, Chaoyuan Jiang, Xiangang Sun, Zhiwen Xu, Ling Zhu
Summary: This article presents a rapid detection method for studying the epidemiology of JEV in pigs in Sichuan province, and finds a high prevalence of JEV GI strains in the area.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Wen Song, Zhiyuan Yin, Xinyu Lu, Danyu Shen, Daolong Dou
Summary: This study found that three plant secondary metabolites (citral, carvacrol, and trans-2-decenal) exhibited strong antimicrobial effects against Phytophthora capsici. Citral at a low concentration restricted P. capsici infection in Nicotiana benthamiana but had no effect on mycelial growth. At a high concentration, citral affected mycelial growth, spore germination, and cell membrane permeability. Additionally, citral inhibited the expression of virulence-related genes in P. capsici and regulated the expression of effector genes during the infection process.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Agronomy
Aaron Barraza, Rosalia Nunez-Pastrana, Abraham Loera-Muro, Thelma Castellanos, Carlos Julian Aguilar-Martinez, Isaac Salvador Sanchez-Sotelo, Maria Goretty Caamal-Chan
Summary: This study investigated the molecular mechanisms underlying the interaction between Phytophthora capsici and Capsicum chinense, a susceptible cultivar. Three elicitors were used to induce early defense in the plants, and the expression of defense-related genes was analyzed. The results showed that MeJA and BABA elicitors reduced disease symptoms and up-regulated genes related to the JA/ET signaling pathway. This suggests that JA signaling plays an important role in the early defense against P. capsici.
Review
Medicine, General & Internal
Kanny Diallo, Vitalis F. Feteh, Lilian Ibe, Martin Antonio, Dominique A. Caugant, Mignon du Plessis, Ala-Eddine Deghmane, Ian M. Feavers, Katya Fernandez, LeAnne M. Fox, Charlene M. C. Rodrigues, Olivier Ronveaux, Muhamed-Kheir Taha, Xin Wang, Angela B. Brueggemann, Martin C. J. Maiden, Odile B. Harrison
Summary: Bacterial meningitis is a significant global cause of morbidity and mortality, and rapid identification of the causative agent is crucial for clinical management and disease prevention. Molecular diagnostic assays have been developed as an alternative to microbiological culture, allowing for faster and more accurate detection of the pathogens causing bacterial meningitis.
Article
Biochemistry & Molecular Biology
Wei Cheng, Menglan Lin, Moli Chu, Guixiang Xiang, Jianwen Guo, Yan Jiang, Deyi Guan, Shuilin He
Summary: The study found that using RNA interference technology to target RXLR effector genes can effectively inhibit the infection of Phytophthora capsici, which is of great significance for the prevention and control of Phytophthora blight disease.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Plant Sciences
Alejandro Ayala-Donas, Pedro Gomez, Miguel de Cara-Garcia
Summary: Differential gene expression was found between genotypes, tissues, days post-inoculation, and inoculated/non-inoculated samples. After inoculation, CpPAL and CpChiIV were downregulated in MUCU-16, while CpLPOX and CpDEF were upregulated in M63. CpDEF showed higher overexpression after inoculation, indicating its potential as a marker for biotic stress.
Article
Microbiology
Joung-Ho Lee, Muhammad Irfan Siddique, Jin-Kyung Kwon, Byoung-Cheorl Kang
Summary: Phytophthora capsici is an oomycete pathogen responsible for various diseases in vegetable and legume crops. Genomic analysis of three isolates with different aggressiveness levels revealed differences and accelerated evolution in pathogenicity-related genes. Resequencing showed frequent accumulation of non-synonymous substitutions in these genes, with expansion signals in more aggressive isolates. This study provides insight into genomic features related to the evolution and pathogenicity of P. capsici.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Sherif A. El-Kafrawy, Mai M. El-Daly, Ahmed M. Hassan, Steve M. Harakeh, Thamir A. Alandijany, Esam Azhar
Summary: In this study, a rapid, simple, and sensitive RT-LAMP assay for the detection of SARS-CoV-2 in clinical samples was established. The assay is suitable for point of care detection in public hospitals, medical centers in rural areas, and in transportation hubs.
Article
Chemistry, Multidisciplinary
Cancan Yang, Zhenling Huang, Xiuguo Zhang, Chunyuan Zhu
Summary: Soluble quinone oxidoreductases facilitate electron transfer from NADPH to quinones for detoxification of synthetic compounds. The crystal structure of NADPH-dependent QOR from Phytophthora capsici (Pc) reveals a bimodular architecture with NADPH-binding grooves and substrate-binding pockets. The enzyme functions as a tetramer in solution and exhibits conserved topology among homologous structures, but with varying substrate specificities. PcQOR prefers catalyzing large substrates like 9,10-phenanthrenequinone, potentially aiding in detoxification of harmful chemicals during invasion by P. capsici.
Article
Biochemistry & Molecular Biology
Chengchun Lai, Qiuxia Que, Ruo Pan, Qi Wang, Huiying Gao, Xuefang Guan, Jianmei Che, Gongti Lai
Summary: This study investigated the disease resistance mechanism in Vitis vinifera and Vitis pseudoreticulata plants. The researchers identified the role of a single-stranded DNA- and RNA-binding protein gene called Whirly in disease resistance. Their findings suggested that the transcription of the Whirly gene was influenced by fungal infection and promoter sequences, leading to the regulation of disease resistance genes and enhanced resistance to pathogens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Anna La Torre, Yari De Santis, Valerio Battaglia, Elisa Costantini, Patrizio Pulcini, Alessandro Polito
Summary: This study evaluated the effectiveness of several natural products for the control of Phytophthora infestans and Phytophthora capsici, and found that they can effectively inhibit the growth and spore germination of these pathogens, as well as reduce disease severity. These findings provide important guidance for the sustainable management of plant diseases.
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Chemistry, Analytical
Sishuo Song, Fan Ji, Qingdong Zeng, Xiaoteng Ding, Jiahao Lin, Xinsheng Wang, Yuanhong Xu, Ruijie Deng, Jinghong Li
Summary: Fungicide abuse can lead to the emergence of fungicide-resistant fungal pathogens, posing a threat to agriculture and food safety. In this study, researchers developed iARMS, an isothermal amplification refractory mutation system, which enables rapid and sensitive detection of fungicide-resistant crop fungal pathogens. The iARMS assay showed high specificity and sensitivity, and it was able to detect rare fungicide-resistant isolates.
ANALYTICAL CHEMISTRY
(2023)